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acellular nerve basal lamina tube
相关语句
  去细胞神经基膜管
     Acellular nerve basal lamina tube; Basic fibroblast growth factor(bFGF); Schwann cells
     结论 bFGF及肝素与许旺细胞、去细胞神经基膜管构成的复合型组织工程神经桥接体修复神经缺损能提高神经再生质量。
短句来源
  “acellular nerve basal lamina tube”译为未确定词的双语例句
     Result The parameter in group A(including the density of regenerative myelinated fibers, dimeter of myelinated fibers, thickness of myelin sheath, the motor nerve conduction velocity, and muscle wet weight) were not statistically different with group D ( P >0.05). Conclusion The nerve bridge compounded of bFGF heparin, acellular nerve basal lamina tube and Schwann cells can facilitate nerve regeneration. [Key words] Peripheral nerve;
     结果 去细胞基膜管种植许旺细胞并复合bFGF及Hep的桥接体组 (A组 )神经再生及功能指标 (再生有髓神经纤维密度、平均髓鞘厚度、有髓纤维直径、运动神经传导速度、肌肉湿重恢复率 )与自体神经移植 (D组 )比较 ,差异无显著性 (P >0 .0 5 )。
短句来源
  相似匹配句对
     Acellular nerve basal lamina tube; Basic fibroblast growth factor(bFGF); Schwann cells
     结论 bFGF及肝素与许旺细胞、去细胞神经基膜管构成的复合型组织工程神经桥接体修复神经缺损能提高神经再生质量。
短句来源
     An experimental study of repairing nerve gap with acellular basal lamina tubes
     去细胞异体神经基膜管桥接神经缺损的实验研究
短句来源
     REPAIR OF PERIPHERAL NERVE DEFECT BY XENOGENEIC ACELLULAR NERVE BASAL LAMINA SCAFFOLDS
     异种神经基膜管桥接周围神经缺损的初步研究
短句来源
     The morphological study of two kinds of acellular basal Lamina tubes of sciatic nerve
     大鼠坐骨神经去细胞基膜管的组织形态学研究
短句来源
     Experimental study on repairing sciatic nerve defects by two kinds of acellular basal lamina grafts
     大鼠坐骨神经去细胞基膜管异体移植的比较研究
短句来源
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Objective To develop an effective new material for repair of nerve defects. Methods The 4 week pre degenerative and normal sciatic nerve segments of SD rats were extracted with the detergent lysophosphatidylcholine, which resulted in acellular nerve basal lamina tubes, called EDNG (extracted degenerative nerve grafts) and ENG (extracted normal nerve grafts) respectively. The morphology of the acellular nerve basal lamina tubes was revealed...

Objective To develop an effective new material for repair of nerve defects. Methods The 4 week pre degenerative and normal sciatic nerve segments of SD rats were extracted with the detergent lysophosphatidylcholine, which resulted in acellular nerve basal lamina tubes, called EDNG (extracted degenerative nerve grafts) and ENG (extracted normal nerve grafts) respectively. The morphology of the acellular nerve basal lamina tubes was revealed by conventional histological examinations, transmission electron microscopy and immunohistochemistry method. The basal lamina tubes, as allografts, were used to repair a 15 mm gap of sciatic nerve in Wistar rats. In the control group nerve autografts (NAG) was applied. Evaluation included general observation, electromyographic examination, muscle measurement and histological observation of serial sections at 12 weeks after surgery. Results The EDNG had more ideal frame, better resilience than ENG. There was no any cell debris in EDNG, whereas in the inner wall of the tubes immunohistochemical analysis revealed clear positive staining and strong immunoreactivity of laminin under immunoelectron microscopy. After peripheral nerves were repaired, all the animals of the three groups had restoration of function to a certain extent. The result of the NAG group was the best, the EDNG group was the next and the ENG group was the inferior. Conclusion This new material offers a possible solution to repair of a short defect of a peripheral nerve.

目的 探索修复周围神经缺损的新的有效替代材料。方法 将异体的预变性神经和正常神经经溶血卵磷脂裂解液处理后 ,得到一种没有细胞及细胞碎片的、空的神经基膜管 ,将其用来修复大鼠 15mm坐骨神经缺损 ,通过一般观察、肌萎缩测量、电生理检测、连续切片组织学观察和计算机图像分析来评价神经再生。结果 化学抽提的预变性神经和正常神经桥接物组均获得了密集的神经再生和良好的神经功能恢复 ,其中前者效果更为优越。结论 这种材料极有可能成为自体神经的替代材料应用于临床较短的神经缺损的修复

Objective To investigate the effect of constructed artificial nerves compound of bFGF and heparin in repairing rabbits the nerve defects. Method Forty eight adult New Zealand rabbits were random divided into four groups(group A,B,C and D)and the models of injured median nerve of 30mm gap were made. Median nerve defects were repaired in each group. In group A, the artificial nerves composed of acellular nerve...

Objective To investigate the effect of constructed artificial nerves compound of bFGF and heparin in repairing rabbits the nerve defects. Method Forty eight adult New Zealand rabbits were random divided into four groups(group A,B,C and D)and the models of injured median nerve of 30mm gap were made. Median nerve defects were repaired in each group. In group A, the artificial nerves composed of acellular nerve basal lamina tube, neonatal Schwann cells, bFGF and heparin were used to repair the nerve defects. In group B, the bridge consist of basal lamina tube and neonatal Schwann cells were used and group C exogenous bFGF and heparin were trapped in basal lamina tube bridges group D which used autologous nerve grafts served as controls. l month and 3 month postoperatively, the regeneration of the nerve bridges of each group were observed including general observation, histological examination and morphologic analysis, electrophysiological test and muscle wet weight resume rate. Result The parameter in group A(including the density of regenerative myelinated fibers, dimeter of myelinated fibers, thickness of myelin sheath, the motor nerve conduction velocity, and muscle wet weight) were not statistically different with group D ( P >0.05). Conclusion The nerve bridge compounded of bFGF heparin, acellular nerve basal lamina tube and Schwann cells can facilitate nerve regeneration. [Key words] Peripheral nerve; Tissue engineer; Acellular nerve basal lamina tube; Basic fibroblast growth factor(bFGF); Schwann cells

目的 研究碱性成纤维细胞生长因子 (bFGF)和肝素与乳兔许旺细胞、去细胞基膜管、构成的复合型组织工程化外周神经桥接体修复兔正中神经缺损的效果。 方法 新西兰兔 48只 ,建立左侧上臂正中神经 3 0mm缺损模型 ,随机分为 4组 ,分别用去细胞基膜管种植许旺细胞并复合bFGF及肝素 (Hep)的桥接体 (A组 )、去细胞基膜管种植许旺细胞的桥接体 (B组 )、去细胞基膜管复合bFGF及Hep桥接体 (C组 )、自体神经 (D组 )修复神经缺损 ,于术后 1、3个月分别进行大体观察 ,Masson三色染色光镜观察神经再生、神经内胶原纤维形成及血管形成 ,3个月检测各组桥接体运动神经传导速度 ,并行透射电镜检查 ,称量指浅屈肌肌肉湿重 ,观察神经功能恢复。 结果 去细胞基膜管种植许旺细胞并复合bFGF及Hep的桥接体组 (A组 )神经再生及功能指标 (再生有髓神经纤维密度、平均髓鞘厚度、有髓纤维直径、运动神经传导速度、肌肉湿重恢复率 )与自体神经移植 (D组 )比较 ,差异无显著性 (P >0 .0 5 )。 结论 bFGF及肝素与许旺细胞、去细胞神经基膜管构成的复合型组织工程神经桥接体修复神经缺损能提高神经再...

目的 研究碱性成纤维细胞生长因子 (bFGF)和肝素与乳兔许旺细胞、去细胞基膜管、构成的复合型组织工程化外周神经桥接体修复兔正中神经缺损的效果。 方法 新西兰兔 48只 ,建立左侧上臂正中神经 3 0mm缺损模型 ,随机分为 4组 ,分别用去细胞基膜管种植许旺细胞并复合bFGF及肝素 (Hep)的桥接体 (A组 )、去细胞基膜管种植许旺细胞的桥接体 (B组 )、去细胞基膜管复合bFGF及Hep桥接体 (C组 )、自体神经 (D组 )修复神经缺损 ,于术后 1、3个月分别进行大体观察 ,Masson三色染色光镜观察神经再生、神经内胶原纤维形成及血管形成 ,3个月检测各组桥接体运动神经传导速度 ,并行透射电镜检查 ,称量指浅屈肌肌肉湿重 ,观察神经功能恢复。 结果 去细胞基膜管种植许旺细胞并复合bFGF及Hep的桥接体组 (A组 )神经再生及功能指标 (再生有髓神经纤维密度、平均髓鞘厚度、有髓纤维直径、运动神经传导速度、肌肉湿重恢复率 )与自体神经移植 (D组 )比较 ,差异无显著性 (P >0 .0 5 )。 结论 bFGF及肝素与许旺细胞、去细胞神经基膜管构成的复合型组织工程神经桥接体修复神经缺损能提高神经再生质量。

Objective:To investigate the function of bFGF and heparin compounding within nerve bridges in repairing rabbit median nerve defects.Methods:48 adult male New Zealand rabbits were randomly divided into four groups (group A,B,C and D).The models of injured median nerve of 30mm gap were created and repaired by various nerve bridges,which were composed of acellular nerve basal lamina tube,neonatal schwann cells and bFGF and heparin in group A;basal lamina tube and neonatal schwann cells in...

Objective:To investigate the function of bFGF and heparin compounding within nerve bridges in repairing rabbit median nerve defects.Methods:48 adult male New Zealand rabbits were randomly divided into four groups (group A,B,C and D).The models of injured median nerve of 30mm gap were created and repaired by various nerve bridges,which were composed of acellular nerve basal lamina tube,neonatal schwann cells and bFGF and heparin in group A;basal lamina tube and neonatal schwann cells in group B; and exogenous bFGFF,heparin and basal lamina tube bridges in group C.Group D that used autologous nerve grafts served as control.In one and three months after operation,the regeneration,collagen formation and revascularization in each nerve bridge were observed by histological examination,including hematoxylin-eosin staining and Masson’s stains.The electrophysiological test,transmission electron microscopy,morphologic analysis,and muscle wet weight and nerve functional recovery were also carried out and evaluated with in 3 months.Results:The bridges in groupA and group C became well vascularizion and had less collagen fibrosis than that in group B (P<0.01),but no statistic difference comparing with group D(P>0.05).The parameters of group A,including the density of regenerative myelinated fibers,diameter of myelinated fibers,thickness of myelin sheath, the motor nerve conduction velocity and muscle wet weight,were not statistical difference comparing with those of control group (P>0.05).Conclusion:bFGF and heparin could improve nerve regeneration by inducing revascularization of the tissue engineering nerve bridge,enhancing the growth of axons and decreasing the formation of collagen fibrosis.

目的:探讨bFGF和肝素与外周神经桥接体复合后在兔正中神经缺损修复中的作用。方法:成年雄性新西兰兔48只,建立左侧上臂正中神经30mm缺损。随机分为4组,分别用不同神经桥接体修复神经缺损,即A组为去细胞基膜管种植雪旺细胞并复合bFGF及肝素的桥接体、B组为去细胞基膜管种植雪旺细胞的桥接体、C组为去细胞基膜管复合bFGF及Hep桥接体、D组为自体神经作为对照。于术后1月、3月分别取材行HE及Masson’s三色染色,光镜观察神经再生、神经内胶原纤维形成及血管形成;3月检测各组桥接体运动神经传导速度,并行透射电镜检查,称量指浅屈肌肌肉湿重,观察神经功能恢复。结果:复合bFGF及肝素的桥接体组(A组、C组)神经再血管化及神经胶原形成与自体神经差异无统计学意义(P>0.05),与B组比差异有显著统计学意义(P<0.01)。A组神经再生数据(再生有髓神经纤维密度、平均髓鞘厚度、有髓纤维直径及运动神经传导速度、肌肉湿重恢复率)与自体神经移植(D组)比较,差异无统计学意义(P>0.05)。结论:bFGF及肝素用于组织工程神经桥接体修复神经缺损,能提高神经桥接体再血管化水平,减少胶原纤维形成,促进轴突生长有利于神经再生质量的...

目的:探讨bFGF和肝素与外周神经桥接体复合后在兔正中神经缺损修复中的作用。方法:成年雄性新西兰兔48只,建立左侧上臂正中神经30mm缺损。随机分为4组,分别用不同神经桥接体修复神经缺损,即A组为去细胞基膜管种植雪旺细胞并复合bFGF及肝素的桥接体、B组为去细胞基膜管种植雪旺细胞的桥接体、C组为去细胞基膜管复合bFGF及Hep桥接体、D组为自体神经作为对照。于术后1月、3月分别取材行HE及Masson’s三色染色,光镜观察神经再生、神经内胶原纤维形成及血管形成;3月检测各组桥接体运动神经传导速度,并行透射电镜检查,称量指浅屈肌肌肉湿重,观察神经功能恢复。结果:复合bFGF及肝素的桥接体组(A组、C组)神经再血管化及神经胶原形成与自体神经差异无统计学意义(P>0.05),与B组比差异有显著统计学意义(P<0.01)。A组神经再生数据(再生有髓神经纤维密度、平均髓鞘厚度、有髓纤维直径及运动神经传导速度、肌肉湿重恢复率)与自体神经移植(D组)比较,差异无统计学意义(P>0.05)。结论:bFGF及肝素用于组织工程神经桥接体修复神经缺损,能提高神经桥接体再血管化水平,减少胶原纤维形成,促进轴突生长有利于神经再生质量的提高。

 
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