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shiga like toxin type
相关语句
  类志贺氏菌毒素
     Expression and Identification of the Subunit B Genes of Shiga like toxin typeⅡ Variant
     类志贺氏菌毒素Ⅱ型变异体B亚单位基因的表达与鉴定
短句来源
     AMPLIFYING, CLONING AND SEQUENCING OF THE SUBUNITS GENES OF THE SHIGA LIKE TOXIN TYPE Ⅱ VARIANT
     类志贺氏菌毒素型变异体基因的克隆与鉴定
短句来源
  “shiga like toxin type”译为未确定词的双语例句
     Cloning and Expression of the A Subunit Gene of the Shiga like Toxin Type II Variant
     猪水肿病毒素A亚单位基因的克隆及表达的尝试
短句来源
  相似匹配句对
     Progress in Shiga Like Toxin Research(a Review)
     志贺样毒素的研究进展
短句来源
     Marriage is like...
     婚姻如螺丝
短句来源
     I LIKE CHINA
     我喜欢中国
短句来源
     Results There were total 46 strains STEC to produce Shiga-like toxin.
     结果 各种来源产志贺样毒素的大肠杆菌共46株,O157型23株,其余为非O157型.
短句来源
     Preparation and Application of Monoclonal Antibodies against Shiga like Toxin Ⅱ Variant
     志贺氏菌样毒素Ⅱ型变异体单克隆抗体的制备及其应用
短句来源
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  shiga like toxin type
Polymerase chain reaction amplification, cloning and sequencing of variant Escherichia coli Shiga-like toxin type II operons.
      
Cloning and sequencing of a Shiga-like toxin type II variant from Escherichia coli strain responsible for edema disease of swine.
      


Edema disease (ED) of weaning piglets is caused mainly by Verotoxigenic Escherichia coli (VTEC). Fimbriae F18ab and Shiga-like toxin type II variant are two pathogenic factors of VTEC. In this research, five strains of E.coli were isolated from the swine which suffered from edema disease and all of them were testified to be O_(139) serum type.Two of the five strains were detected,which can expressed fimbriae F18ab by using the monoclonal antibody of fimbriae F18ab, and the data...

Edema disease (ED) of weaning piglets is caused mainly by Verotoxigenic Escherichia coli (VTEC). Fimbriae F18ab and Shiga-like toxin type II variant are two pathogenic factors of VTEC. In this research, five strains of E.coli were isolated from the swine which suffered from edema disease and all of them were testified to be O_(139) serum type.Two of the five strains were detected,which can expressed fimbriae F18ab by using the monoclonal antibody of fimbriae F18ab, and the data of PCR also showed that this two stains contain the SLT-IIe genes. Twelve antibiotics were tested and the two strains were highly sensitive to four antibiotics,including norfloxacin, tobramycin, gentamycin and rifampin.

本实验从疑似猪水肿病的病例分离到 5株大肠杆菌 ,O抗原鉴定结果表明所有菌株均为O1 3 9血清型 ;应用 F18ab菌毛单克隆抗体对这 5株大肠杆菌能否表达 F18ab菌毛进行了鉴定 ,结果表明其中 2个菌株能表达 F18ab菌毛 ;利用聚合酶链式反应 ( PCR)对志贺氏菌样毒素 型变异体 ( SL T- e)操纵子基因保守区进行了扩增 ,结果发现在能表达 F 18ab菌毛 2个菌株中可扩增一段特异性序列。以上数据表明这 2株大肠杆菌为致仔猪水肿病大肠杆菌。药敏试验表明这两株菌株均对氟哌酸、妥布霉素、庆大霉素、利福平等抗生素高度敏感

The 298-805 bp and the 818-1 201 bp fragments of A subunit gene of Shiga-like toxin (typeⅡ) variant (SLT-Ⅱe A gene) were amplified by PCR, and the 806-817 bp fragment of the latter fragment was deleted by PCR. The PCR products were cloned into prokaryotic expression vector pGEX-6P-1 and the recombinant plasmid pGEX-A_(de) was constructed. The recombinant plasmid was transformed into E.coli BL21. SDS-PAGE and Western-blotting tests confirmed that the fusion protein named GST-ⅡeA had...

The 298-805 bp and the 818-1 201 bp fragments of A subunit gene of Shiga-like toxin (typeⅡ) variant (SLT-Ⅱe A gene) were amplified by PCR, and the 806-817 bp fragment of the latter fragment was deleted by PCR. The PCR products were cloned into prokaryotic expression vector pGEX-6P-1 and the recombinant plasmid pGEX-A_(de) was constructed. The recombinant plasmid was transformed into E.coli BL21. SDS-PAGE and Western-blotting tests confirmed that the fusion protein named GST-ⅡeA had been expressed in E.coli BL21 from the SLT-ⅡeA gene.

采用PCR方法分别对类志贺氏菌毒素A亚单位基因第 2 98~ 80 5序列、818~ 12 0 1序列进行了扩增 ,使其第 80 6~ 817序列的碱基 (编码 16 7~ 170位氨基酸 )缺失 ,并将两片段连接后克隆至质粒载体pGEX 6P 1中 ,获得了含有重组质粒 pGEX Ade的重组大肠埃希氏菌 ;经SDS PAGE以及使用特异性抗SLT ⅡeA单克隆抗体的Western blotting ,证明该重组大肠埃希氏菌在IPTG诱导条件下可表达SLT ⅡeA。

Shiga-like toxin typeⅡvariant (SLT-Ⅱe) is composed of one A subunit and five B subunits. The A subunit possesses RNA N-glycosidase activity, which specifically removes an adenine in the 28S subunit of eukaryotic rRNA, leading to inactivation of the cellular protein synthesis and disorganization of metabolic functions of the cell. The double points mutated at amino acid residues 167(Glu-Gln) and 170(Arg-Lys) of SLT-IIeA by the technology of SOE (Splicing by overlap extension). The product...

Shiga-like toxin typeⅡvariant (SLT-Ⅱe) is composed of one A subunit and five B subunits. The A subunit possesses RNA N-glycosidase activity, which specifically removes an adenine in the 28S subunit of eukaryotic rRNA, leading to inactivation of the cellular protein synthesis and disorganization of metabolic functions of the cell. The double points mutated at amino acid residues 167(Glu-Gln) and 170(Arg-Lys) of SLT-IIeA by the technology of SOE (Splicing by overlap extension). The product was cloned into prokaryotic expression vector pGEX-6P-1 and resulting recombinant was called pGEX-A_(mu). The recombinant plasmid was transformed into E.coli BL21 and GST-fusion protein (GST-ⅡeA) with respected size was expressed by Western-blotting using monoclonal antibody specific for SLT-ⅡeA.

利用重叠延伸PCR法对志贺样毒素 型变异体A亚单位(SLT- eA)基因进行修饰扩增,将第167位编码Glu的密码子突变为Gln的密码子,第170位编码Arg的密码子突变为编码Lys密码子,并用质粒载体pGEX-6P-1在大肠杆菌BL21中进行融合表达,在不改变其蛋白空间构象的同时降低其毒性。重组SLT- eA突变株pGEX-Amu的大量表达为研究SLT- eA在体内的生物学特性提供依据。

 
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