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super expression
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  超表达
     Bcl-2and bax are important controlling genes for apoptosis. Super expression of bcl-2makes cell survival longer,but super expression of bax makes cell apoptosis.
     bcl-2、bax是细胞凋亡的重要调控基因,bcl鄄2的超表达使细胞生存期延长,而bax的超表达则使细胞凋亡。
短句来源
  超强表达
     Transformation of Apple Using Super Expression Cowpea Trypsin Inhibitor (CpTI) Gene
     超强表达豇豆胰蛋白酶抑制剂基因(CpTI)转化苹果的研究
短句来源
     Cowpea trypsin inhibitor(CpTI)gene in super expression binary vector PECp was transferred to apple cultivar‘Gala’using Agrobacterium mediated leaf disc transformation method.
     通过农杆菌介导法将超强表达载体PECp中的豇豆胰蛋白酶抑制剂基因CpTI转入了苹果品种‘嘎拉’。
短句来源
  “super expression”译为未确定词的双语例句
     Conclusion: NF-KB and P21 proteins are super expression and interact to regulate antiapoptosis, the proliferation and the malignant transformation of leukemic cells.
     结论:白血病细胞中NF-κB及P21蛋白异常高表达,2者偶联活化,共同参与了细胞的恶性转化和增殖。
短句来源
     Red ginseng can restrain the super expression of extracellular matrix(FN、LN) and E-selction.
     (3)红参可抑制细胞外基质成分(FN、LN)过度表达,从而减轻毛细血管基底膜的损伤。 (4)红参可抑制视网膜血管E-选择素的表达,从而抑制白细胞与内皮细胞的粘附,减轻毛细血管内皮的损伤。
短句来源
     Super expression of Fas gene may play a key role in inducing opoptosis of gastric mucosa of H.pylori related chronic gastritis in children.
     Fas基因过度表达可能是 H.py-lori诱导胃粘膜上皮细胞凋亡增加的机制之一 .
短句来源
     Conclusion AAⅠ can injure the renal tubular epithelial cell directly and induce the expression of VEGF,bFGF and TGF-β_1.The early and super expression of VEGF,bFGF and TGF-β_1 in the renal tubular epithelial cells may be the important cause of renal tubular interstitial lesion and interstitial fibrosis.
     结论马兜铃酸可以刺激致纤维化细胞因子VEGF、bFGF和TGF-β在肾小管上皮细胞内表达。 VEGF、bFGF、TGF-β的早期、过度表达可加重马兜铃酸肾病的进展。
短句来源
  相似匹配句对
     The expression of E.
     研究了E.
短句来源
     The expression
     COX-2、VEGF-C mRNA的表达与肿瘤TNM
短句来源
     The Super Car
     V2卡比号超能汽车
短句来源
     Super Animals
     超级动物秀
短句来源
     Super-expression of neurons' bcl-2 gene can prevent neurons from apoptosis.
     eQ基因在宇q经系统也有表达,3和空元对beLZ的过莹彭胡u神经元的凋亡。
短句来源
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AIM To investigate the change of gastric epithelial cell apoptosis and Fas gene in children with H.pylori associated chronic gastritis. METHODS Gastric epithelial cell apoptosis and Fas gene of thirty six chronic gastritis children (19 H.pylori negative and 17 H.pylori positive before and after anti H.pylori treatment) were studied by TUNEL and Envision Immunohistochemical staining. RESULTS Apoptotic and Fas indexes of 19 H.pylori negative children were (2.7±0.8)% and (11.7±9.0)%...

AIM To investigate the change of gastric epithelial cell apoptosis and Fas gene in children with H.pylori associated chronic gastritis. METHODS Gastric epithelial cell apoptosis and Fas gene of thirty six chronic gastritis children (19 H.pylori negative and 17 H.pylori positive before and after anti H.pylori treatment) were studied by TUNEL and Envision Immunohistochemical staining. RESULTS Apoptotic and Fas indexes of 19 H.pylori negative children were (2.7±0.8)% and (11.7±9.0)% respectively; Apoptotic and Fas indexes of 17 H.pylori positive children were (8.5±1.8)% and (31.3±11.9)% respectively. The difference was significant ( P <0.01). Apoptotic and Fas of 15 H.pylori positive gastritis patients were (8.6±2.3)% and (32.5 ±12.7)% before eradication, and were (3.6±1.8)% and (13.3 ±6.4)% after eradication. The apoptotic and Fas index decreased significantly following H.pylori eradication ( P <0.01). CONCLUSION H.pylori infection might enhance gastric mucosa cell apoptosis. Super expression of Fas gene may play a key role in inducing opoptosis of gastric mucosa of H.pylori related chronic gastritis in children.

目的 探讨幽门螺杆菌 (H .pylori)相关性小儿慢性胃炎胃粘膜上皮细胞凋亡和相关基因 Fas的变化 .方法 采用 TUNEL技术和 Envision免疫组化方法检测 19例 H .py-lori阴性慢性胃炎和 17例 H.pylori阳性慢性胃炎患儿 H.pylori清除前后胃粘膜上皮细胞凋亡和相关基因 Fas的变化 .结果  19例 H.pylori阴性小儿胃炎胃粘膜上皮细胞凋亡指数和 Fas指数分别为 (2 .7± 0 .8) %和 (11.7± 9.0 ) % ;17例 H.pylori阳性小儿慢性胃炎胃粘膜凋亡指数和 Fas指数分别为 (8.5± 1.8) %和 (31.3± 1.9) % ;H.pylori阳性组凋亡指数和 Fas指数明显高于 H .pylori阴性组 P<0 .0 1. H .py-lori清除后胃粘膜上皮凋亡指数和 Fas指数均明显减少(P<0 .0 1) ,治疗前凋亡指数和 Fas指数分别为 (8.6± 2 .3) %和 (32 .6± 12 .7) % ;清除 H.pylori后 ,凋亡和 Fas指数分别为 (3.6± 1.8) %和 (13.3± 6 .4) % .结...

目的 探讨幽门螺杆菌 (H .pylori)相关性小儿慢性胃炎胃粘膜上皮细胞凋亡和相关基因 Fas的变化 .方法 采用 TUNEL技术和 Envision免疫组化方法检测 19例 H .py-lori阴性慢性胃炎和 17例 H.pylori阳性慢性胃炎患儿 H.pylori清除前后胃粘膜上皮细胞凋亡和相关基因 Fas的变化 .结果  19例 H.pylori阴性小儿胃炎胃粘膜上皮细胞凋亡指数和 Fas指数分别为 (2 .7± 0 .8) %和 (11.7± 9.0 ) % ;17例 H.pylori阳性小儿慢性胃炎胃粘膜凋亡指数和 Fas指数分别为 (8.5± 1.8) %和 (31.3± 1.9) % ;H.pylori阳性组凋亡指数和 Fas指数明显高于 H .pylori阴性组 P<0 .0 1. H .py-lori清除后胃粘膜上皮凋亡指数和 Fas指数均明显减少(P<0 .0 1) ,治疗前凋亡指数和 Fas指数分别为 (8.6± 2 .3) %和 (32 .6± 12 .7) % ;清除 H.pylori后 ,凋亡和 Fas指数分别为 (3.6± 1.8) %和 (13.3± 6 .4) % .结论  H .pylori感染促进胃粘膜上皮细胞的凋亡 ;Fas基因过度表达可能是 H.py-lori诱导胃粘膜上皮细胞凋亡增加的机制之一 .

Cowpea trypsin inhibitor(CpTI)gene in super expression binary vector PECp was transferred to apple cultivar‘Gala’using Agrobacterium mediated leaf disc transformation method.GUS staining and southern blot confirmed that CpTI gene was inserted into apple genome.

通过农杆菌介导法将超强表达载体PECp中的豇豆胰蛋白酶抑制剂基因CpTI转入了苹果品种‘嘎拉’。GUS检测和Southern杂交结果证明CpTI基因已经整合进苹果基因组

β-1,3-glucanase( BG2 )is one of the pathogensis-related-proteins(PR).Study of these proteins and their related genes is one of the hot points in plant genetic engineering of disease resistance for a long time.In this research,specific primers were designed with the enzyme cleavage site of Spe Ⅰ in its forward one and Not Ⅰ site in the backward according to the BG2 gene sequence.Using this pair of primers, BG2 gene,which was contained in the plasmid of pRTL2,was amplified and confirmed by sequencing the amplified...

β-1,3-glucanase( BG2 )is one of the pathogensis-related-proteins(PR).Study of these proteins and their related genes is one of the hot points in plant genetic engineering of disease resistance for a long time.In this research,specific primers were designed with the enzyme cleavage site of Spe Ⅰ in its forward one and Not Ⅰ site in the backward according to the BG2 gene sequence.Using this pair of primers, BG2 gene,which was contained in the plasmid of pRTL2,was amplified and confirmed by sequencing the amplified fragment inserted into T-easy vector.The positive clone containing BG2 gene was digested with the enzymes of Spe Ⅰ/ Not Ⅰ and then BG2 gene was inserted into the Xba Ⅰ/ Not Ⅰ sites of super expression binary vector pATC940.The reconstructed expression vector named as pATCBG2 was introduced into the wheat of Longfumai10 and Longfumai3 ( Triticum aestivum L.em.Thell) through the particle gun transformation method.The Kanamysin resistant (Km r) transformants were obtained.PCR,Dot-blotting and PCR-Southern hybridization analysis showed that the BG2 gene was integrated into the genome of wheat.Result of pathogen inoculation assay on the transgenic plants showed that the transgenic plants had a higher resistant disease score of 1~2 grade than the control.

利用PCR方法设计引物 ,进行特异扩增 ,在得到的BG2基因片段的两端引入可以与表达载体多克隆位点相匹配的酶切位点 ,将该基因插入高效表达载体质粒pATC94 0中 ,获得表达质粒pATCBG2。通过基因枪转化法 ,将pATCBG2转化优质小麦品种龙辐麦 10、龙辐麦 3号 ,获得抗卡那霉素 (Kanamycin)的再生植株 ,经PCR ,PCR Southern和Dot blotting检测 ,结果表明 ,有 5个转基因植株在以上各项检测中全为阳性 ,证明目的基因已整合入这些转基因小麦基因组中。田间接菌发病检测结果表明 ,转基因植株比对照的抗病性提高 1~ 2级。

 
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