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osteochondral graft
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  osteochondral graft
Step-off edges and tissue interfaces are prevalent in cartilage injury such as after intra-articular fracture and reduction, and in focal defects and surgical repair procedures such as osteochondral graft implantation.
      
Large autogenous osteochondral graft for replacing knee cartilage defect
      
In 60-day (group A) and 90-day (group B) old rabbits a standardized osteochondral graft was taken from the distal articular surface of the femur and replanted immediately.
      
Therefore, an autogenous osteochondral graft is considered a good method in the treatment of knees with moderately sized articular cartilage defects.
      
The TM joint was reconstructed with an osteochondral graft from the second metatarsal head or an ear cartilage graft sutured on top of the fibula.
      
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Objective:To study the new method of osteochondral autografts.Methods:Making 32 white healthy bigear adult rabbits as animal models,by the wedgeshaped with thick layers on osteochondral grafts,to finish unloaded cartilages substituted loaded cartilages.Results:The grafted cartilages can firmly unionize with their recepters,both the space and activity of cartilages being normal,the newly born cartilage tending to be normal.The result of the measurement of tritated thymidin incorporation those no obvious...

Objective:To study the new method of osteochondral autografts.Methods:Making 32 white healthy bigear adult rabbits as animal models,by the wedgeshaped with thick layers on osteochondral grafts,to finish unloaded cartilages substituted loaded cartilages.Results:The grafted cartilages can firmly unionize with their recepters,both the space and activity of cartilages being normal,the newly born cartilage tending to be normal.The result of the measurement of tritated thymidin incorporation those no obvious dedine of cartilage cells activity.Conclusion:Its resurfacing articular cartilage defects with unloaded cartilages substituted loaded cartilages is reliable.Therefore a new method is offered to the material choice of articular cartilage grafts.

目的:探讨自体骨软骨移植取材的新方法。方法 :选用健康成年大耳白兔32只 ,用楔形带厚层骨的骨软骨移植方法 ,取自体非负重区软骨替代负重区软骨进行修复试验。结果 :移植的软骨与受区牢固愈合 ,关节间隙正常 ,关节活动度正常 ,新生软骨为透明软骨。3H -胸腺嘧啶掺入法测试 :软骨细胞活性无明显降低。结论 :自体非负重区软骨替代负重区软骨修复关节软骨缺损疗效肯定 ,在关节软骨移植取材方面提供了新方法。

Objective To discuss the repair methods for the cartilage defects of the knee joint. Methods The cylindrical osteochondral grafts were harvested from the non-weight-bearing area of the knee joint and then transplanted to the cartilage defects in 6 patients with cartilage defects of the knee joint. Results The clinical symptoms of all the patients followed up for 2-24 months disappeared and the joint mobility recovered to normal. A follow-up MRI showed consistent cartilage coverage and the excellent...

Objective To discuss the repair methods for the cartilage defects of the knee joint. Methods The cylindrical osteochondral grafts were harvested from the non-weight-bearing area of the knee joint and then transplanted to the cartilage defects in 6 patients with cartilage defects of the knee joint. Results The clinical symptoms of all the patients followed up for 2-24 months disappeared and the joint mobility recovered to normal. A follow-up MRI showed consistent cartilage coverage and the excellent position of the cylindrical osteochondral grafts. Conclusions The osteochondral autograft is a practical surgical method because it is characterized by less trauma, simple performance and good maintenance of the curvature of the articular surface.

目的 探讨膝关节软骨缺损的修复方法。 方法  6例膝关节软骨缺损患者 ,关节镜下在其非负重区的软骨面上 ,用专用器械凿取圆柱状的骨软骨 ,并移植至软骨缺损部位 ,用于修复缺损。 结果 随访 2~ 2 4个月 ,患者的临床症状消失 ,关节活动度正常 ,MRI显示原软骨缺损区软骨表面平整 ,移植的骨软骨柱位置良好。 结论 自体镶嵌式骨软骨移植术创伤小、操作简单、能保持关节面的曲度 ,是较为实用的手术。

Aim investigate the change of hyaline cartilage cells and marrow stromal cells in the different local microenvironment respectively. Methods Two osteochondral grafts,2.5 mm in diameter and 3.0 mm in deep were obtained from the femural trochlea articular surface of 9 New Zealand white rabbits.The grafts were deep enough into the subchondral cancellous bone,or into the bone marrow plainly,and the autografts were then implanted upside down in site,namely,the articular cartilage faced marrow...

Aim investigate the change of hyaline cartilage cells and marrow stromal cells in the different local microenvironment respectively. Methods Two osteochondral grafts,2.5 mm in diameter and 3.0 mm in deep were obtained from the femural trochlea articular surface of 9 New Zealand white rabbits.The grafts were deep enough into the subchondral cancellous bone,or into the bone marrow plainly,and the autografts were then implanted upside down in site,namely,the articular cartilage faced marrow and managed to keep the surface which had approached bone marrow smooth.The animals were killed at 4,8,12 weeks postoperative respectively.Evaluation included gross appearance of the healed grafts,histological investigation using haematoxylin and erosin and Safranin O staining and with immunohistochemical staining for type II collagen.Results At 4 weeks postoperatively,the surface of inversion was covered with thin fibrous tissue, with the margins clearly defined.At 8 and 12 weeks after operation,a relative smooth articular surface was restored,and the margins of the autografts were not clearly distinguishable.By this time,fibrocartilage was observed at the reparative surfacing of upside down grafts while the original articular cartilage still almost kept their inherent phenotype.Conclusion MSCs with cancellous bone being scaffold fails to repair articular cartilage.Undifferentiated bone marrow derived osteochondral progenitor cells offer a possible alternative to repair cartilage defect if their capacity is enhanced to differentiate into cartilage with the technology of cytokines induction or gene modification.

目的应用关节镜灌洗术、软骨下钻孔术、骨膜或软骨膜移植等方法治疗关节软骨损伤,透明软骨得不到真正的修复。因此以自体或异体软骨细胞、骨髓基质细胞为种子细胞修复关节软骨并观察关节透明软骨细胞和骨髓基质细胞在体内不同微环境中的变化。方法新西兰兔9只,在股骨髁髌骨滑车关节面钻取两个直径2.5mm、深3mm的骨软骨块,将软骨面向下、软骨下骨向上原位倒置,分别于4,8,12周取材,做大体观察和苏木精-伊红(HE)染色、safranin-O染色、II型胶原免疫组化。结果4周时倒置体表面有纤维组织覆盖,原关节软骨细胞形态结构正常。8~12周,倒置体表面有纤维软骨再生,原关节透明软骨基本保持其原有特性。结论以骨松质为支架的骨髓基质细胞未能完全修复关节软骨损伤。修复关节软骨最好采用同一组织来源的透明软骨细胞。

 
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