助手标题  
全文文献 工具书 数字 学术定义 翻译助手 学术趋势 更多
查询帮助
意见反馈
   antibody probe 的翻译结果: 查询用时:0.009秒
图标索引 在分类学科中查询
所有学科
基础医学
更多类别查询

图标索引 历史查询
 

antibody probe
相关语句
  抗体探针
     coli JM109 appeared good antigenicity to the specific antibody probe.
     经用特异性抗体探针检测,证明该片段在大肠杆菌中的表达产物具有良好的抗原性
短句来源
     Screening of Schistosoma Japonicum Adult Worm cDNA Library by Antibody Probe
     用抗体探针筛选日本血吸虫成虫cDNA文库
短句来源
     Preparation of Rabbit Antibody Probe and Qualitative Analysis Specific for Flammulin
     火菇素抗体探针的制备与高灵敏度的火菇素定性技术的建立
短句来源
     It is the first report of obtaining partial sequences of plant disease resistance gene by using antibody probe in the world, which provides a basis of cloning the whole gene encoding API protein.
     在国内外首次利用抗体探针获得了植物抗病蛋白编码基因的部分序列,为进一步克隆全长的API蛋白基因奠定了基础。
短句来源
     The lysatic bacterial cells were separated by SDS_PAGE,the 4A7 monoclonal antibody probe against C. jejuniCA specifically recognized the 39.0kD protein of recombinant expression bacteria on western blots. The lysate derived from expressing 39.0kD proteins E.
     将表达重组子的菌体裂解产物进行SDS_PAGE ,并用免疫印迹对表达的抗原用 4A7单抗体探针进行反应 ,结果与 39kD的表达特异蛋白发生特异性免疫反应。
短句来源
更多       
  “antibody probe”译为未确定词的双语例句
     In vitro synthesis of F protein gene from a Chinese virulent strain of NDV and its antigenicity to specific antibody probe
     我国新城疫强毒株F糖蛋白基因的体外合成及其抗原性研究
短句来源
     Methods:Bone marrow epithelial membrane antigen(EMA) positive cells from patients with breast cancer were measured by immunocytochemistry using an antibody probe.
     方法 :采用上皮膜抗原 ( EMA)单克隆抗体为探针 ,用免疫组化 ABC法检测乳腺癌患者骨髓中 EMA表达阳性细胞。
短句来源
     A cDNA entry library and a cDNA expression library of Alternaria spp. JH505 were constructed with in vitro site-specific recombination technique, and a full-length coding sequence of plant activator protein was obtained by screening the expression library with antibody probe.
     本研究利用体外特异位点重组反应成功地构建了链格孢菌JH505菌株cDNA入门文库和表达文库,并通过免疫筛选从中得到编码植物激活蛋白的核酸序列。
短句来源
     Methods S.japonicum adult worm cDNA expression library was screened with Sj31/32 McAb as a catching antibody probe. The positive clone 312 was introduced as lysogens into E. coli Y1089 and induced by IPTG.
     方法 日本血吸虫 31/32kDa单克隆抗体免疫筛选的阳性克隆 312经IPTG诱导表达 ,表达产物rSj31/ 32加福氏佐剂免疫小鼠后进行攻击感染实验。
短句来源
     Methods:The bone marrow epithelial membrane antigen (EMA) positive cells from patients with lung cancers were measured by immunocytochemical assay by an antibody probe.
     方法 :用免疫组化法检测肺癌患者骨髓上皮膜抗原 (EMA)阳性表达细胞 ,分析其与肺癌病理类型、分期及远处转移的关系。
短句来源
更多       
  相似匹配句对
     2 antibody as the probe.
     2抗体进行Western杂交的结果证明二者均为孵化酶分子。
短句来源
     Synthesis of Novel Monoclonal Antibody Fluorescent Probe Dyes
     新型单克隆抗体荧光探针染料的合成
     antibody drug
     抗体药物
     Probe into "Kiev
     “基辅”号航母探秘
短句来源
     On Probe Into Teaching
     略论探究教学
短句来源
查询“antibody probe”译词为用户自定义的双语例句

    我想查看译文中含有:的双语例句
例句
为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法,我们为您准备了出自英文原文的大量英语例句,供您参考。
  antibody probe
The original proteins and subsequent antibody probe reactions are performed directly on the surface.
      
This technique is modified from that originally published by Cassab and Varner (J Cell Biol 105: 2581-2588, 1987) for the localization of extensin protein in soybean seed using an antibody probe.
      
These characteristics make PG-4 a useful monoclonal antibody probe to identify the total population of proteoglycans in human skin.
      
Rational selection of an antibody probe to detect the heterogeneous collection of CHO-derived rhGM-CSF glycoforms
      
The remaining proteoglycan fragments were detected using a monoclonal antibody probe directed against the chondroitin sulfate (CS) region of the core protein.
      
更多          


A cDNA expression library of adult Schistosoma japonicum (Chinese mainland strain) was constructed in bacteriophage expression vector λgt11. The library was approximately 5×10~5 re- combinants in size,as judged by color selection with IPTG and X-Gal. Pooled sera taken from hu- man individuals chronically infecfed with S. japonicum were used as a catching antibody probe in an ELISA assay for screening the library. Nine positive plaques were obtained from 15000 plaques. Eight crude lysates generated from...

A cDNA expression library of adult Schistosoma japonicum (Chinese mainland strain) was constructed in bacteriophage expression vector λgt11. The library was approximately 5×10~5 re- combinants in size,as judged by color selection with IPTG and X-Gal. Pooled sera taken from hu- man individuals chronically infecfed with S. japonicum were used as a catching antibody probe in an ELISA assay for screening the library. Nine positive plaques were obtained from 15000 plaques. Eight crude lysates generated from λgt11 recombinant lysogen in E. coli Y1089 were an- alyzed by SDS-PAGE and Western blotting. Two clones expressed fusion proteins of> 116kD, which could be recognized by infected human sera in Western blot analysis.

作者构建了一个日本血吸虫(中国大陆株)成虫cDNA表达库,该库的大小含有5×10~5重组体。采用慢性感染血吸虫病人混合血清对重组空斑进行免疫学筛选,已初筛出9个阳性斑,其中8个克隆的溶源表达产物,经SDS-PAGE表明,有2个克隆各表达了分子量大于116kD的融合蛋白,在Western blot分析中均被血吸虫病人血清所识别。

The antigens produced by recombinant λgt11 plaques were transferred to nitrocellulose filters. Clones encoding given antigens were detected with infected rabbit sera(IRS) which were preabsorbed with NC filters as a catching antibody probe. Twenty-three putative positives were obtained from 6×105 plaque forming units(pfu) in the primary screening and were rescreened a second time,eight of them consistently gave good positive signals in subsequent screenings. The results indicated that the immunoscreening...

The antigens produced by recombinant λgt11 plaques were transferred to nitrocellulose filters. Clones encoding given antigens were detected with infected rabbit sera(IRS) which were preabsorbed with NC filters as a catching antibody probe. Twenty-three putative positives were obtained from 6×105 plaque forming units(pfu) in the primary screening and were rescreened a second time,eight of them consistently gave good positive signals in subsequent screenings. The results indicated that the immunoscreening method described here was able to efficiently isolate single cbnes encoding S. japonicum antigens. The method has the advantages of screening libraries efficiently,reliably and specifically. This might open the way to screen genes encoding S. japonicum antigens inducing protective immunity.

本文将重组体噬菌斑的表达抗原转移至硝酸纤维素膜上,用经假筛选法纯化的日本血吸虫感染兔血清(IRS)来检测。结果6×10~5个噬菌斑形成单位(pfu)初筛出23个可能阳性噬菌斑,经再次筛选,其中有8个克隆持续出现阳性。结果表明该免疫筛选能有效地分离单个编码日本血吸虫抗原的克隆,筛选效率高,特异性强,不需同位素标记技术,操作简便,为进一步筛选编码诱导日本血吸虫保护性免疫力的抗原基因打下了基础。

A 1.9kb of F protein gene fragment was amplified from a virulent strain (F 48 E 9) of NDV isolated in China by RT PCR.Recombinants were obtained after the F protein gene fragment was inserted in pUC19 DNA between the Hind Ⅲ and Sal Ⅰ sites and transformed into E.coli JM109.In the identification trials,the same F protein gene fragment could be amplified by PCR and cleaved by Hind Ⅲ and Sal Ⅰ from the recombinant DNA.Using an improved...

A 1.9kb of F protein gene fragment was amplified from a virulent strain (F 48 E 9) of NDV isolated in China by RT PCR.Recombinants were obtained after the F protein gene fragment was inserted in pUC19 DNA between the Hind Ⅲ and Sal Ⅰ sites and transformed into E.coli JM109.In the identification trials,the same F protein gene fragment could be amplified by PCR and cleaved by Hind Ⅲ and Sal Ⅰ from the recombinant DNA.Using an improved method of colony hybridiztion in situ,the positive clones of E.coli JM109 appeared good antigenicity to the specific antibody probe.

将我国新城疫强毒株F48E9在SPF鸡胚增殖后提取RNA,用自行设计的一对寡聚核苷酸引物进行RT-PCR,合成了F糖蛋白基因片段。将此片段与pUC19质粒重组,转化大肠杆菌JM109,从阳性克隆中提取重组质粒,再用PCR法扩增,获得了同样大小的DNA片段。经用特异性抗体探针检测,证明该片段在大肠杆菌中的表达产物具有良好的抗原性

 
<< 更多相关文摘    
图标索引 相关查询

 


 
CNKI小工具
在英文学术搜索中查有关antibody probe的内容
在知识搜索中查有关antibody probe的内容
在数字搜索中查有关antibody probe的内容
在概念知识元中查有关antibody probe的内容
在学术趋势中查有关antibody probe的内容
 
 

CNKI主页设CNKI翻译助手为主页 | 收藏CNKI翻译助手 | 广告服务 | 英文学术搜索
版权图标  2008 CNKI-中国知网
京ICP证040431号 互联网出版许可证 新出网证(京)字008号
北京市公安局海淀分局 备案号:110 1081725
版权图标 2008中国知网(cnki) 中国学术期刊(光盘版)电子杂志社