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flow gel
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  “flow gel”译为未确定词的双语例句
     The renatured THANK was purified to homogeneity with Q Sephadex Fast Flow gel filtration and then desalted by Sephadex G-25 chromatography.
     复性后组分经 Q Sapharose Fast Flow离子交换层析再次纯化 ,最后以 Sep hadex G- 2 5脱盐。
短句来源
     Three components, named ESPS_0, ESPS_ 1.0 and ESPS_ 1.6, were separated from extracellular polysaccharide (ESPS) of Porphyridium sp. by NaCl gradient elution with Q Sepharose Fast Flow gel.
     采用离子交换凝胶QSepharoseFastFlow,以NaCl梯度洗脱,将紫球藻胞外多糖 (ESPS)分离成3个组分ESPS0、ESPS1.0和ESPS1.6,分别占粗多糖总量的44.2%(以质量 计,下同),20.3%和16.4%.
短句来源
     METHOD Fresh frozen plasma was adsorbed successively by domestic DEAE Sepharose Fast Flow gel and Ca 3(PO 4) 2 , and then solvent detergent (S/D) treatment was used.
     方法 以人新鲜冰冻血浆为原料 ,采用SD病毒灭活工艺 ,经国产DEAE SepharoseFastFlow胶批式吸附和Ca3 (PO4) 2 吸附制备凝血因子Ⅸ复合物。
短句来源
  相似匹配句对
     ON FLOW PROPERTIES OF ZIRCONIUM GEL FRACTURING FLUID
     锆冻胶压裂液管流特性的实验研究
短句来源
     FLOW RESISTANCE OF COLLOIDAL DISPERSION GEL IN POROUS MEDIA
     胶态分散凝胶在多孔介质中的流动阻力
短句来源
     the flow is designed.
     进行了系统的流程设计。
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     in traffic flow.
     交通事故导致的交通堵塞等等。
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     gel migration 12;
     凝胶移位12例;
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  flow gel
It was demonstrated that the countercurrent water flow has significant effect on the freezing progress of the phantom gel in comparison with that of non-flow gel.
      
The isolation procedure includes precipitation at low pH, followed by sedimentation at 110,000 g and reverse flow gel chromatography on Sephadex G-200.
      


LDV is used to measure the velocity profiles and near wall velocities fora dilatant flow-gel 107 solution flow in a slit. After some modification in the Poiseuille flow formulas has been made for side-wall effect correction, the results of LDV measurements combined with bulk parameters are utilized to analyse the enhancement evidence and quantities of the flow, The feasibility of using LDV to investigate flow enhancement phenomenon in detail is demonstrated.

用激光多普勒测速法(LDV)测量了一种胀流型流体——107 胶溶液的狭缝流的速度剖面和近壁速度。在对 Poiseuille’s 流的公式作有关侧壁影响的修正后,将 LDV 的测量结果和总体参量测量结果结合,分析了该流动的增强的迹象和数量。表明用 LDV 详细研究流动增强现象是可行的。

Objective: To prepare highly purified THANK protein. Methods: THANK was efficiently expressed in E.coli as inclusion bodies. After bacteria were lysed under ultrasonication, TE buffer,1% TritonX and 2 mol /L urea was used to efficiently extract inclusion bodies. After denaturation with 8 mol/L urea,THANK was partially purified by Sephacryl S-200 chromatography, and then subsequent refolding step was optimized for a maximal recovery of biological active protein. The renatured THANK was purified to homogeneity...

Objective: To prepare highly purified THANK protein. Methods: THANK was efficiently expressed in E.coli as inclusion bodies. After bacteria were lysed under ultrasonication, TE buffer,1% TritonX and 2 mol /L urea was used to efficiently extract inclusion bodies. After denaturation with 8 mol/L urea,THANK was partially purified by Sephacryl S-200 chromatography, and then subsequent refolding step was optimized for a maximal recovery of biological active protein. The renatured THANK was purified to homogeneity with Q Sephadex Fast Flow gel filtration and then desalted by Sephadex G-25 chromatography. Results: The purity of biologically active THANK was above 97% in SDS-PAGE densitometric studies. Conclusion: An effective method of denaturation, renaturation and purification of recombinant THANK is established.

目的 :获得较纯的具有生物学活性的 THANK蛋白。方法 :THANK基因在大肠杆菌中高效表达 ,菌体超声破碎后 ,以洗涤剂反复洗涤包涵体。包涵体经 8mol/L尿素变性溶解后 ,再用 Sephacryl S- 2 0 0凝胶过滤层析初步纯化 ,对重组蛋白浓度、氧化还原剂等复性参数进行优化和选择 ,将蛋白稀释复性。复性后组分经 Q Sapharose Fast Flow离子交换层析再次纯化 ,最后以 Sep hadex G- 2 5脱盐。 结果 :得到了纯度 >97%、具有一定生物学活性的 THANK蛋白。 结论 :THANK蛋白变性、复性及纯化方法的建立 ,为 THANK活性蛋白的制备提供了依据

OBJECTIVE To produce factor Ⅸ(FⅨ) complex concentrates with high purity and low potential thrombogenicity. METHOD Fresh frozen plasma was adsorbed successively by domestic DEAE Sepharose Fast Flow gel and Ca 3(PO 4) 2 , and then solvent detergent (S/D) treatment was used.RESULTS The activity recovery rates of the two adsorption processes were (89.94±1.31)%( n =7)and (82.27±2.18)% ( n =6). The specific activity of the product is (16.28±2.80)IU/mg. The content of the FⅨ was (126.82±11.60) IU/200PE....

OBJECTIVE To produce factor Ⅸ(FⅨ) complex concentrates with high purity and low potential thrombogenicity. METHOD Fresh frozen plasma was adsorbed successively by domestic DEAE Sepharose Fast Flow gel and Ca 3(PO 4) 2 , and then solvent detergent (S/D) treatment was used.RESULTS The activity recovery rates of the two adsorption processes were (89.94±1.31)%( n =7)and (82.27±2.18)% ( n =6). The specific activity of the product is (16.28±2.80)IU/mg. The content of the FⅨ was (126.82±11.60) IU/200PE. CONCLUSION This method is highly practicable, and it can be used in producing high quality Factor Ⅸ complex concentrates in low cost.

目的 制备纯度较高 ,潜在血栓性小的凝血因子Ⅸ (FⅨ )复合物。方法 以人新鲜冰冻血浆为原料 ,采用SD病毒灭活工艺 ,经国产DEAE SepharoseFastFlow胶批式吸附和Ca3 (PO4) 2 吸附制备凝血因子Ⅸ复合物。结果 两步的活性收率分别为 ( 89.94± 1.31) % (n =7)、( 82 .2 7± 2 .18) % (n =6 ) ,制品的FⅨ∶C比活为 ( 16 .2 8± 2 .80 )IU/mg。FIX的量为 ( 12 6 .82±11.6 0 )IU/瓶 ( 2 0 0PE)。结论 本工艺实用性较高 ,可用于制备低成本 ,高质量的凝血因子Ⅸ复合物。

 
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