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  “5 ' deletion”译为未确定词的双语例句
     In the present work, the seed-specific promoter LegA gene is choosen to construct a set of 5' deletion expression vectors named leg0029I, leg0029III, leg0179I, leg0179III.
     本实验选择种子特异性启动子—LegA基因的启动子作为研究对像,构建了5’端系列缺失表达载体分别命名为:leg0029Ⅰ,leg0029Ⅲ,leg0179Ⅰ,leg0179Ⅲ。
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     The 5' deletion analysis of the Osgrp-2 promoter in tobacco transient expression systems revealed the presence of a positive regulatory region (-2290 to -1406) and two negative regulatory regions (-2401/-2291 and -1405/-1022) for the promoter activity.
     在烟草原生质体瞬间表达系统中对其2.4 Kb的启动子序列所做的系列缺失分析表明,该启动子的活性同时受到正调控片段(-2290/-1406)和负调控片段(-2401/-2291, -1405/-1022)的影响。
短句来源
     Analysis of the 5' deletion constructs showed the regulation region of its basic transcription was mainly within - 1520 to - 1431 bp upstream of ATG Two stem-loop structures were within - 1108 to - 1079 and - 891 to - 864 bp relative to ATG It suggests that their protein binding site is between - 1079 to - 897 bp relative to ATG.
     利用DNAMAN软件分析,在-1108至-1079bp和-891至-864bp之间存在着两个茎环结构,推测茎环结合蛋白位点可能位于-1079至-897bp之间,且ATG上游-388至-86bp之间可能存在乙酰苯胺类化合物诱导元件。
短句来源
     Method: The possibility that BMP-2 stimulates Osterix expression had been detected at transcription level by Real-Time Q-PCR. As a first step toward localizing important control elements within the Osterix promoter, 9 different fragment of 5' deletion mutants were amplified by PCR form the BAC clone BAC-RP23-118K10. The mutant promoters were inserted upstream of the luciferase gene in luciferase report vector pGL3 Basic.
     用Real-Time Q-PCR的方法检测出在转录水平上BMP-2能诱导Osterix的表达上调,然后用PCR的方法,从BAC-RP23-118K10克隆中,扩增出一系列不同长度的Osterix启动子片断,将上述片断克隆到Luciferase报告载体pGL3-Basic Vector中,构建出一系列Osterix启动子缺失突变的报告载体。
短句来源
     The OsRac2 promoter 5' deletion plant expression recombinants which 1.7 kb upstream the transcriptional start site were constructed by PCR amplification,and then transformed into tobacco.
     采用PCR扩增的方法,构建了OsRac2基因转录起始位点上游1.7 kb的启动子5′缺失植物表达载体,转化烟草并筛选阳性转基因植株.
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  相似匹配句对
     5. C.
     5.短跗秽蝇C.
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     (5).
     5.脑GABA浓度:(1).
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     p53 deletion connects with chemoresistance of TAX and 5FU.
     p53突变与PDD耐受相关,p53缺失与TAX、5FU耐受相关。
短句来源
     Nobody was detectable of deletion of exon 5 and exon 8 ;
     在32例急性白血病患者中,无PTEN基因第5,第8外显子同时缺失的患者,对照组PTEN基因第5,第8外显子全部存在;
短句来源
     The heterologies include 5' end alternation, deletion, insertion and so on.
     与HSD-1相比,也发现了一些变异情况。 包括核苷酸序列缺失、5′端变异等。
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  deletion
5' deletion and mutation analyses identified two cAMP response element (CRE)-like sequences (CRE1 and CRE2) that were essential for cAMP-induced promoter II activity.
      
We have stably transfected MCF-7 human breast cancer cells with a mammalian expression vector for the exon 5 deletion variant ER.
      
P53mutation was confirmed only in 1 patient with pTaG2 tumor in exon 5(deletion of proline 128).
      
A series of 5' deletion and linker-scan mutagenesis constructs identified two separate enhancer elements.
      
5' deletion of a gbss1 promoter region from wheat leads to changes in tissue and developmental specificities
      
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A procedure of simultaneous calculations for correcting regression coefficients due to deletion and/or addition of observations is developed, which includes the Plackett result as a special case. Considerations with respect to deleting observations in relation to the missing values problem in the factorial experiment lead to a different, yet feasible, approach to the correction problem. That is, with the deleted observations being first estimated, the correct regression coefficients turn up naturally.As...

A procedure of simultaneous calculations for correcting regression coefficients due to deletion and/or addition of observations is developed, which includes the Plackett result as a special case. Considerations with respect to deleting observations in relation to the missing values problem in the factorial experiment lead to a different, yet feasible, approach to the correction problem. That is, with the deleted observations being first estimated, the correct regression coefficients turn up naturally.As for estimation of missing values, the principle of least squares may be worked into a tabular form equivalent in the wide sense to the method of cross-contrasts as was proposed in a previous paper by one of the authors.

本文导出关于舍去或(并)加入数据的回归计算公式(包括了Plackett的结果作为特例)。并在舍去数据方面,结合析因试验中缺数的估计问题,得到回归计算的另一种可行方法——先补缺,后回归。 根据交互对比法与最小二乘法的等效性,按最小二乘法补缺,可表格化为一般意义的交互对比法。

Chromosome abnormalities were studied in a girl with multiple congenital anomalies. Trisomy of the short arm of chromosome 9 (9p +) and distal deletion of the long arm of X-chromosome were found with G banding and C-banding techniques. The clinical features included psychomotor and growth retardation, short stature, moderate microcephaly, hypertelorism and enophtha mos, prominent nose with inverted nostrils, short upper lip, low-set ears, low posterior hairline, anomalies of phalanges and vertebrae, widely-spaced...

Chromosome abnormalities were studied in a girl with multiple congenital anomalies. Trisomy of the short arm of chromosome 9 (9p +) and distal deletion of the long arm of X-chromosome were found with G banding and C-banding techniques. The clinical features included psychomotor and growth retardation, short stature, moderate microcephaly, hypertelorism and enophtha mos, prominent nose with inverted nostrils, short upper lip, low-set ears, low posterior hairline, anomalies of phalanges and vertebrae, widely-spaced hypoplastic nipples, undeveloped breasts, delayed onset of menar-che and dermatoglyphic abnormalities.

本文报告一例多发性先天畸形女子的细胞遗传学研究。用G带和C带技术鉴定为第9号染色体短臂三体(9p+)和一条X染色体长臂远端部分缺失(xq—)。其临床征状为:严重的智力和发育障碍,身材矮小,头略小,两眼距稍宽并内陷,鼻圆,鼻梁高而鼻孔内翻,上唇短,耳位和后发线低下,指骨和脊椎骨异常,两乳头距远,乳房发育差,月经延迟,皮纹异常。

Transposon Tn233 (CH) contains sir sul-resistant genes which are originally located on drug-resistant plasmid DR233 (Tcr Cmr Smr Sur) harbored by Shigella flexneri strain 233.Plasmid E144drd3: :Tn233 (CH) was constructed previously by transposition of Tn233 (CH) from DE233 into R144drd3 (Km1) and was then transferred to E.coli C600/pBK322 (Apr Tcr),so that strain with two coexisting plasmids was constructed.The plasmid DNA prepared from this coexisting strain was used to transform E.coli C600 cells and was selected...

Transposon Tn233 (CH) contains sir sul-resistant genes which are originally located on drug-resistant plasmid DR233 (Tcr Cmr Smr Sur) harbored by Shigella flexneri strain 233.Plasmid E144drd3: :Tn233 (CH) was constructed previously by transposition of Tn233 (CH) from DE233 into R144drd3 (Km1) and was then transferred to E.coli C600/pBK322 (Apr Tcr),so that strain with two coexisting plasmids was constructed.The plasmid DNA prepared from this coexisting strain was used to transform E.coli C600 cells and was selected for Tc resistant transformants on L-broth plate containing 12.5ug Tc per ml.After the transformants had grown on Tc containing plates,E.coli C600/pBR322::Tn233 (CH) strain was selected from these colonies on the same medium containing 12.5ug/ml Sm by replica method.Then plasmid DNA was extracted from E.coli C600/pBR322:: Tn233 (CH) cells.The purified plasmid DNA preparations were digested by restriction endonuclease BamHI,EcoRⅡ,PstI,HindⅢ,PvuII and subjected to electrophoresis through agarose horizontal gel slabs and polyacrylamide gel in Tris-Acetate or Loening bouffer using DNA digested by both BamHⅠ and EcoRI,T5 DNA digested by Hindlll and M13 DNA digested by HaeⅢ as mobility markers.The molecular weight of these DNA fragments were measured.The molecular weight of plasmid pBR322:: Tn233 (CH) calculated by summation of all the bands to be of approximately 15.93×106 daltons,assuming that there is no deletion on pBR322 during the transposition experiment.If we take pBR322 as 2.87X106 daltons,the molecular weight of Tn233 (CH) is 13.06×106 daltons.The electrophoresis results also indicated that the number of substrate site of restriction endonuclease BamHI,EcoRI,PstI,HindⅢ and PvuⅡ on the Tn233 (CH) DNA sequence were 5,9,1,6 and 2 respectively.

转座子Tn233(CH)带有str sul抗性基因,最早是在痢疾杆菌的抗药质粒DR233(Tc~r Cm~r Sm~r Su~r)中发现的。现在通过菌株间的配对,将插入了Tn233(CH)转座子的质粒R144drd3::Tn233(CH)转移到E·coli C600/pBR322(Ap~r、Tc~r)细胞中,组成两种质粒共存的菌株。从此菌株中提取出质粒DNA,用转化方法使它转移到E.coli C600菌株,再从所得到的转化子中用复印方法筛选出Tn233(CH)转座到pBR322质粒的转化子E.coli C600/pBR322::Tn233(CH),然后提出此质粒DNA,经限制性内切酶BamHⅠ、EcoRⅠ、PstⅠ、HindⅢ与PvuⅡ等酶切后,在琼脂糖凝胶平板与聚丙烯酰胺凝胶柱上进行电泳分析,分别以BamHⅠ与EcoRⅠ双重酶解的λDNA、HindⅢ酶解的T5DNA、HaeⅢ酶解的M13 DNA与HaeⅢ酶解的pBR322 DNA作为泳动的标记,计算出质粒酶解片段的分子量,用此方法算出各片段分子量的总和为15.93×10~6道尔顿,此即为所求的pBR322::Tn233(CH)分子量,将此值减去pBR322...

转座子Tn233(CH)带有str sul抗性基因,最早是在痢疾杆菌的抗药质粒DR233(Tc~r Cm~r Sm~r Su~r)中发现的。现在通过菌株间的配对,将插入了Tn233(CH)转座子的质粒R144drd3::Tn233(CH)转移到E·coli C600/pBR322(Ap~r、Tc~r)细胞中,组成两种质粒共存的菌株。从此菌株中提取出质粒DNA,用转化方法使它转移到E.coli C600菌株,再从所得到的转化子中用复印方法筛选出Tn233(CH)转座到pBR322质粒的转化子E.coli C600/pBR322::Tn233(CH),然后提出此质粒DNA,经限制性内切酶BamHⅠ、EcoRⅠ、PstⅠ、HindⅢ与PvuⅡ等酶切后,在琼脂糖凝胶平板与聚丙烯酰胺凝胶柱上进行电泳分析,分别以BamHⅠ与EcoRⅠ双重酶解的λDNA、HindⅢ酶解的T5DNA、HaeⅢ酶解的M13 DNA与HaeⅢ酶解的pBR322 DNA作为泳动的标记,计算出质粒酶解片段的分子量,用此方法算出各片段分子量的总和为15.93×10~6道尔顿,此即为所求的pBR322::Tn233(CH)分子量,将此值减去pBR322的分子置2.87×10~6道尔顿,得到Tn233(CH)的分子量为13.06×10~6道尔顿。电泳结果还表明在Tn233(CH)DNA分子上,BamHⅠ、EcoRⅠ、PstⅠ、HindⅢ与PvuⅡ分别有5、9、1、6、2个切点数。

 
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