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   serine-repeat antigen gene 的翻译结果: 查询用时:0.202秒
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serine-repeat antigen gene
相关语句
  丝氨酸重复抗原基因
     THE EXPRESSION IN E.COLI OF THE SERINE-REPEAT ANTIGEN GENE FROM PLASMODIUM FALCIPARUM.
     恶性疟原虫丝氨酸重复抗原基因在大肠杆菌中的表达
短句来源
  相似匹配句对
     SOLUBILITY STUDIES ON L-SERINE
     L-丝氨酸溶解度的研究
短句来源
     The Studies on Fermentation of L-Serine
     微生物发酵法生产L-丝氨酸的研究
短句来源
     Study on the advance of serine
     丝氨酸的研究进展
短句来源
     SNAKE VENOM SERINE PROTEASES
     蛇毒丝氨酸蛋白酶
短句来源
     Cloning of the glyA gene encoding the serine hydroxymethyltransferase
     丝氨酸羟甲基转移酶基因(glyA)的克隆
短句来源
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The serine repeat antigen gene fragment of the Plasmodium falciparum PFD 3/YN (Yunnan,China) was amplified by polymerase chain reaction. After sequencing,the gene fragment was cloned into pWR450 1 vector for expression of fusion protein with β galactosidase. The recombinant plasmid pWR450 SERA was transformed into the E. cloi TG1 strain.Different concentrations of IPTG were added to different bacteria densities,and the expressions of the β galactosidase SERA fusion proteins in E.coli...

The serine repeat antigen gene fragment of the Plasmodium falciparum PFD 3/YN (Yunnan,China) was amplified by polymerase chain reaction. After sequencing,the gene fragment was cloned into pWR450 1 vector for expression of fusion protein with β galactosidase. The recombinant plasmid pWR450 SERA was transformed into the E. cloi TG1 strain.Different concentrations of IPTG were added to different bacteria densities,and the expressions of the β galactosidase SERA fusion proteins in E.coli were examined. Dot ELISA and Western blot analysis were used to identify the expression products. The results indicated that the recombinant plasmid could be induced to express a 72kDa fusion protein. The fusion protein was expressed at high level when the bactera density reached OD 590 0.8~1.2 and IPTG was added to a final concentration of 1mmol/L. The dot ELISA and Western blot analysis indicated that the expressed fusion protein could react specifically with monoclonal antibody against SERA.

采用PCR方法特异性扩增恶性疟原虫云南株(PFD-3/YN)丝氨酸重复抗原基因片段,经基因序列测定后克隆于pWR450-1融合蛋白表达载体,并转化大肠杆菌TG1。在不同菌体浓度及不同剂量IPTG诱导下检测SERA融合蛋白的表达。采用dot-ELISA及Western-blot分析对表达产物进行鉴定,结果显示SERA基因与pWR450-1重组后在大肠杆菌TG1中表达一72kDa的融合蛋白,当工程菌OD590值为0.8~1.2时,加入终浓度1mmol/LIPTG进行诱导,表达量较高。dot-ELISA和Westernblot分析表明SERA基因表达产物能被抗SERA单克隆抗体所识别。

The serine repeart antigen gene fragment of the plasmodium falciparum PFD-3/YN(Yunan of China) was amplified by polymerase chain reaction.After the gene sequencing,the gene fragment was cloned into pGEX-4T-1 vector for expression of fusion protein with Glutathion S transferase.The recombinant plasmid pGEX SERA was transformed into the E.coli strains TG1.The recombinant plasmid could be induced to express a 45KDa fusion protein in E.coli after serine repeat antigen gene...

The serine repeart antigen gene fragment of the plasmodium falciparum PFD-3/YN(Yunan of China) was amplified by polymerase chain reaction.After the gene sequencing,the gene fragment was cloned into pGEX-4T-1 vector for expression of fusion protein with Glutathion S transferase.The recombinant plasmid pGEX SERA was transformed into the E.coli strains TG1.The recombinant plasmid could be induced to express a 45KDa fusion protein in E.coli after serine repeat antigen gene fragment recombined with pGEX-4T-1 vector.The fusion protein was expressed at high level when the bacterium were culture until the OD 590 reached 0 8~1 0 and added IPTG to a final concentration of 1mmol/L.Using dot ELISA to identify the expression products,the results indicated that expressed fusion protein could react specifically with monoclonal antibody against SERA.

采用 PCR方法特异性扩增恶性疟原虫云南株 (PFD- 3/ YN)丝氨酸重复抗原基因片段 ,经基因序列测定后克隆于 p GEX- 4T- 1融合蛋白表达载体 ,并转化大肠杆菌 TG1。SERA基因与 p GEX- 4T- 1重组后能在大肠杆菌 TG1中表达一 45 KDa的融合蛋白 ,当工程菌 OD5 90 为 0 .8~ 1.0时 ,加入终浓度 1m mol/ L IPTG进行诱导 ,表达量较高。采用 dot- EL ISA对表达产物进行鉴定。结果表明 SERA融合蛋白能被抗 SERA单克隆抗体所识别。

The serine-repeat antigen gene fragment of Plasmodium falciparum FCC-1/HN from the China was amplified by polymerase chain reaction and cloned into M13 bacteriophage. M13-SERA single strand DNAs of the three positively clones was extracted respectively. Then, the nucleotide sequence of the SERA gene fagment, the gene fragment is 453bp and contains high A+T contents (A+T/G+C for 2.6:1), which accorded with the structure gene composition of Plasmodium falciparum. The homology analysis indicated...

The serine-repeat antigen gene fragment of Plasmodium falciparum FCC-1/HN from the China was amplified by polymerase chain reaction and cloned into M13 bacteriophage. M13-SERA single strand DNAs of the three positively clones was extracted respectively. Then, the nucleotide sequence of the SERA gene fagment, the gene fragment is 453bp and contains high A+T contents (A+T/G+C for 2.6:1), which accorded with the structure gene composition of Plasmodium falciparum. The homology analysis indicated that there was only one amino acid difference at the SERA 667 site among Plasmodium falciparum FCC-1/HN (from China), B1, B2, 3 and S16 isolates, there was no difference among Plasmodium falciparum PFD-3/YN, FCR3, FCBR, S14 and S15 isolates. The result indicated that the gene fragment is high conservation in the different isolates. The prediction of the antigenic epitope exhibited that the fragment may be carry antigenic epitopes at N terminal and C terminal.

该文采用PCR方法特异性扩增恶性疟原虫海南株 (FCC - 1 HN)SERA基因片段 ,并将该基因片克隆于M 13噬菌体 ,用双脱氧链末端终止法进行测序 ,应用PCGENE软件对该基因序列进行了分析 ,结果显示该基因片段长度为 45 3bp ,A +T G +C为 2 .6∶1,符合恶性疟原虫结构基因的特点。同源性分析显示该基因在不同虫株间高度保守 ,我国FCC - 1 HN虫株SERA与B1、B2、B3(巴西 )株及S16 (塞内加尔 )株仅一个氨基酸不同 ,与FCR3(刚比亚 )、FCBR(哥伦比亚 )、及S14、S15 (塞内加尔 )等其它虫株SERA基因序列完全一致。抗原表位预测分析显示该多肽N端和C端可能有抗原表位存在

 
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