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   protein fluorescence 的翻译结果: 查询用时:0.204秒
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protein fluorescence
相关语句
  蛋白质荧光
     STUDIES OF QUENCHING OF PROTEIN FLUORESCENCE BY SULPHATED HYPOCRELLIN
     磺化竹红菌素对蛋白质荧光猝灭机理的研究
短句来源
     The experimental results show that the quenching of protein fluorescence by sul-phated Hypocrellin follows the classical Stern-Volmer equation. The variations of otherconditions such as temperatures,viscosities,pH,guanidine-HCl ionic strength were alsoinvestigated.
     结果表明,磺化竹红菌素对多种蛋白质荧光猝灭服从Stern-Volmer曲线,实验观察了温度、粘度、pH值和盐酸胍对荧光猝灭过程的影响。
短句来源
     The effects of pH on the protein fluorescence quenching after interaction with NAR were studied to find that the active functional group in neutral state played key role in the binding process.
     研究了不同pH值条件下药物对蛋白质荧光猝灭的影响,发现药物分子中的没有解离的活性基团在结合过程中发挥着主导作用.
短句来源
  “protein fluorescence”译为未确定词的双语例句
     The results showed that the toxic protein fluorescence intensity is improved with its pH value increase and reaches its maximum at pH equivalent to 11. Adding some organic solvents such as methanol, ethanol, isopropanol result in the emission wavelength of fluorescence of the toxic protein blue shift and its fluorescence intensity increase.
     加入甲醇、乙醇、异丙醇等有机溶剂引起毒蛋白荧光发射波长蓝移和荧光强度增大,至有机溶剂的浓度超过某一阈值后荧光发射波长不再蓝移; 丙酮能完全碎灭毒蛋白CrylAb的荧光,丙烯酸胺能碎灭CrylAb 86%的荧光而KI对毒蛋白天辟灭作用,由此推断 Cry Ah的大部分色氨酸残基位于分子表面富含负电荷的区域中 ;
短句来源
     Measurments of the quenching of the intrinsic protein fluorescence of the lobster muscle glyceraldehyde-3-phosphate dehydrogenase during titration with NAD+ of the native, carboxymethylated and the irradiated enzyme carrying the fluorop hore showed that all 3 forms of the enzyme binding NAD+ with negative cooperativity.
     平衡柱层析法测得每分子龙虾肌羧甲基化甘油醛-3磷酸脱氢酶能结合3.9分子NAD~+,而每分子光照酶则只能结合2分子NAD~+。
短句来源
     The protein fluorescence spectra had no significant change with humidity.
     蛋白紫外荧光光谱随湿度增加未发现有明显变化.
短句来源
     Changes in intrinsic protein fluorescence of yeast GAPDH have been compared with its inactivation during denaturation in GuHCI solutions. The holo-and the apo-enzymes are completely inactivated at GuHCI concentrations less than 0.5M and this is accompanied by marked decrease in flurescence emission intensity and red Shift of the emission maxima at 335 nm suggesting exposure of aromatic residues.
     酵母3-磷酸甘油醛脱氢酶在盐酸胍溶液中的内源荧光及剩余活力的变化结果提示:apo酶及holo酶的活力在胍浓度为0.5M左右可完全丧失. 同时伴有内源荧光强度的下降,光谱宽度的增加和335nm最大发射峰的红移(提示了色氨酸残基的暴露).
短句来源
     Stearylamine and oleylamine. C18 saturated and cis -monounsaturated amines, with positive charges. were found to decline the intensity of the intrinsic protein fluorescence of skeletal muscle sarcoplasmic reticulum (SR) in the absence of ATP.
     在无ATP存在时,带相同正电荷、但饱和度不同的两种胺类两亲物,即C_(18)饱和的硬脂胺与单不饱和的油胺引起肌浆网蛋白内源荧光强度降低.
短句来源
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  相似匹配句对
     Fluorescence of dipyridamole enhanced by protein
     蛋白质对双嘧达莫的荧光增敏作用研究
短句来源
     and the GFP protein was observed with fluorescence.
     荧光显微镜下观察GFP表达情况。
短句来源
     protein.
     protein。
短句来源
     Protein Folding
     蛋白质的折叠
短句来源
     The green fluorescence of E.
     IPTG诱导表达后,在荧光显微镜下可观察到E.
短句来源
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  protein fluorescence
Rates of oxygen consumption and the fade in the protein fluorescence spectrum upon sensitized irradiation were monitored.
      
NADP+ and NADPH, but not isocitrate, cause quenching of protein fluorescence.
      
In the presence of lactate dehydrogenase-X and NAD+, the addition of pyruvate or α-ketovalerate resulted in a time-dependent quenching of protein fluorescence and an increase in absorbance at 325 nm indicating the formation of a ternary complex.
      
Both oxidized and reduced coenzymes induced significant quenching of protein fluorescence.
      
Binding of this aromatic anionic dye causes a quenching of the protein fluorescence and various changes in the spectral properties of the dye.
      
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The UV fluorescence spectra of purple membrane fragments were measured. The fluorescence intensity of protein in the PM samples irradiated with orange light was lower than that of control. The intensities of UV fluorescence of PM and apomembrane were similar, but the quantum yield of UV fluorescence of bacterioopsin was twice as large as that of the natural purple membrane. This result illustrated that the protein fluorescence was possibly quenched by the chromophore of PM. The conversion of the dark-adapted...

The UV fluorescence spectra of purple membrane fragments were measured. The fluorescence intensity of protein in the PM samples irradiated with orange light was lower than that of control. The intensities of UV fluorescence of PM and apomembrane were similar, but the quantum yield of UV fluorescence of bacterioopsin was twice as large as that of the natural purple membrane. This result illustrated that the protein fluorescence was possibly quenched by the chromophore of PM. The conversion of the dark-adapted PM into light-adapted PM was induced by irradiation of UV light at 280nm. The photobleaching of PM was also induced by the UV light in the presence of hydroxylamine. By measuring the action spectrum of photobleaching of PM we have showed that the action spectrum of photobleaching of PM coincided with the absorption spectrum of PM in the UV region.All the results showed that the energy absorbed by protein had transferred to retinal.

以可见光为作用光照射天然紫膜,紫膜蛋白被280nm紫外光激发所发射的荧光强度比对照略有降低.比较天然紫膜、漂白紫膜与菌蛋白三者的紫外荧光强度,前两者无显著变化,但菌蛋白的荧光强度比天然紫膜的荧光强度大2-3倍,表明生色团对蛋白质荧光可能有猝灭作用.用280nm波长光照射紫膜的暗适应形式,可使其转变成光适应形式.若有羟胺存在,以紫外光照射也可使紫膜漂白.光漂白的作用光谱,其紫外部分与紫膜蛋白部分的吸收光谱重合得很好.上述实验证明紫膜蛋白部分吸收的能量可以转移到生色团上,即紫膜存在分子内的能量转移

Currently, methods of utilizing fluorescence in the diagnosis of cancers are roughly divided into two kinds: The method of fluorescence sensibiliza-tion and the method of direct observation of the inherent fluorescence. This paper reported the study of the electronic spectrum of the tissue homogenate of human stomach,and quantitatively investigated the center of luminescence. According to the observation of the inherent fluorescence, an early diagnosis of malignant tumors may be made preliminarily.The tissue...

Currently, methods of utilizing fluorescence in the diagnosis of cancers are roughly divided into two kinds: The method of fluorescence sensibiliza-tion and the method of direct observation of the inherent fluorescence. This paper reported the study of the electronic spectrum of the tissue homogenate of human stomach,and quantitatively investigated the center of luminescence. According to the observation of the inherent fluorescence, an early diagnosis of malignant tumors may be made preliminarily.The tissue homogenate of human stomach has a relatively strong photo-absorption in near ultraviolet and violet areas. The photoatosorption in violet area is the absorption of the purine compound in biologic tissues. It is evident that the electronic spectrum is influenced by the transition of the lea-dins conjugated ρ electrons.It is obvious from the results of the experiment that utilizing ultraviolet light with different wave lengths to excite the gastric homogenate, roughly same integrated protein fluorescence emission peaks were obtained. Comparing this emission peak with that of the tryptophan (348nm), some displacement was found, and this is just the fluorescence characteristic of the protein B. Therefore, it is concluded that under the excitation of ultraviolet light, the photospectrum of aromatic compound is the chief characteristic of that of the gastric homogenate.From the result of the experiment, it is noticed that the emission in red area of the ultraviolet-excited gastric homogenate was basically originated from the transition of the ρ electrons of purine cyclic group of hemoglobin. It possesses distinct common characteristics with ths fluorescence spectrum of the human whole blood, hemoglobin and hematoporphyrin. Therefore, the pho-toemission of gastric homogenate in visible area was chiefly originated from the hemoglobin molecules of the tissues.Once the pathologic lesions developed in tissues, it will inevitably lead to changes in the molecular composition, molecular structure as well as physicochemical environment around the emission center, eausing changes in the state of emission center as a result of changes in inherent fluorescence. In the comparison of the spectrum of inherent fluorescence in normal tissue with that in cancer tissues, it is obvious that there is significant difference between the emission peak of the fluorescence of cancer tissues and that of the normal tissue homogenate.

本文报导了人胃组织匀浆的电子光谱。采用光谱解析法和分子轨道理论分析了匀浆中的发光中心。讨论了人胃组织匀浆的发光特征,并通过与人胃癌组织匀浆荧光光谱的对比分析,观察到了人胃癌组织匀浆的特异光谱。

Measurments of the quenching of the intrinsic protein fluorescence of the lobster muscle glyceraldehyde-3-phosphate dehydrogenase during titration with NAD+ of the native, carboxymethylated and the irradiated enzyme carrying the fluorop hore showed that all 3 forms of the enzyme binding NAD+ with negative cooperativity. Gel permeation studies gave the results that the carboxymethylated enzyme binds 4 molecules of NAD+ per tetrameric enzyme whereas the irradiated enzyme binds only 2. The latter is in accord...

Measurments of the quenching of the intrinsic protein fluorescence of the lobster muscle glyceraldehyde-3-phosphate dehydrogenase during titration with NAD+ of the native, carboxymethylated and the irradiated enzyme carrying the fluorop hore showed that all 3 forms of the enzyme binding NAD+ with negative cooperativity. Gel permeation studies gave the results that the carboxymethylated enzyme binds 4 molecules of NAD+ per tetrameric enzyme whereas the irradiated enzyme binds only 2. The latter is in accord with the results shown in the previous paper that the irradiated lobster enzyme already has 2 molecules of a fluorescent NAD derivative in the NAD+ binding sites of 2 of the subunits. During the entire course of the preparation of the lobster enzyme the pH has been kept below 7, hence the negative cooperativity of the binding of NAD+ to the native as well as to the carboxymethylated enzymes cannot be attributed to slight damage of the enzyme molecule in alightly alkaline medium as has been suggested for the rabbit muscl enzyme.

平衡柱层析法测得每分子龙虾肌羧甲基化甘油醛-3磷酸脱氢酶能结合3.9分子NAD~+,而每分子光照酶则只能结合2分子NAD~+。 由蛋白荧光淬灭法得到,在25℃、pH7.0的磷酸盐缓冲液中,全酶、羧甲基酶及光照酶与NAD~+结合时均呈负协同性。

 
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