助手标题  
全文文献 工具书 数字 学术定义 翻译助手 学术趋势 更多
查询帮助
意见反馈
   rapid electrophoresis 的翻译结果: 查询用时:0.009秒
图标索引 在分类学科中查询
所有学科
临床医学
更多类别查询

图标索引 历史查询
 

rapid electrophoresis
相关语句
  快速电泳
     Methods: Routine blood test and hemoglobin zone quantitative determinatiOn were performed, using full automatic hemocyte analyzer(SYSMEX NE 1500 of the East Asia Company of Japan)for the former, and full automatic rapid electrophoresis(REP) system(HELENA Company of U.S.A) and its pH8.6 agar gel reagent for the latter.
     方法日本东亚公司SYSMEXNE1500全自动血细胞分析仪检测血常规,美国HELENA公司全自动快速电泳系统(REP)及其pH8.6琼脂凝胶试剂作血红蛋白区带定量。
短句来源
     Methods The routine hematological examination was performed with full automatic hemocyte analyzer,the Hb analysis was done with full automatic rapid electrophoresis analysis system (parts of the samples were used for the α1, and β1,Thal genetic analysis),and the serum iron protein was examined with chemiluminescence immunity analyzer.
     方法:用全自动血细胞分析仪检测血常规,全自动快速电泳分析系统作血红蛋白(Hb)分析(部分标本作α、β-Thal基因分析),并用化学发光免疫分析仪检测血清铁蛋白。
短句来源
     Reference Value Assay for a Hundred Healthy Persons'Serum Protein Conpoment by Automatic Rapid Electrophoresis Analyzer
     全自动快速电泳分析系统检测100例健康人血清蛋白组分参考值
短句来源
     Rapid electrophoresis and EcoRI-HindIII digestion analysis showed that most of the recombinant plasmids from the white clones contained insertions with various lengths and 3 out of 4 tested were about 1700,2600 and 1400 bp in length respectively.
     快速电泳检测及酶切分析表明,多数重组子带有插入片段,其中3个重组子的插入片段长度大致为1700 bp、2600 bp和1400 bp。
短句来源
     Methods We use REP automatic rapid electrophoresis system to detect LDH,CK isozymes of 57 patients with dermatomyositis.
     方法 用日本HITACHI 74 7型全自动生化分析仪及美国REP全自动快速电泳系统检测 5 7例皮肌炎患者血清LDH、CK同工酶。
短句来源
更多       
  “rapid electrophoresis”译为未确定词的双语例句
     The positive rate of falciparum malaria blood samples detected by rapid electrophoresis and rapid thin-layer chromatography was 90.00% and 80.00% respectively, vivax malaria was 83.33% and 76.67% respectively as same way.
     检测病人血样的阳性率分别为 90 .0 0 %和 80 .0 0 % ,检测 30份间日疟病人血样的阳性率分别为 83.33%和 76.67% ;
短句来源
     Rapid electrophoresis and restriction endonuclease(BamHl) digestionanalysis showed that the- most of the recombinant plamids from the white clones contained insertions with about 340bp length.
     酶切图谱表明,来自白色菌落的大多数重组质粒含有340bp的插入片段,且克隆到的该片段以正向插入质粒载体。
短句来源
     Rapid electrophoresis of plasmid prepared by alkali method, EcoRI-HindⅢ digestion and in situ hybridization with32P labeled TuMV-RNA showed ihat inserted fragments were with various sizes and complement to TuMV-RNA.
     菌落原位杂交及酶切分析表明,重组质粒中的插入片段大多数为芜菁花叶病毒RNA的互补DNA,长度为500—4000bp。
短句来源
     Methods: A complete blood count (RBC?Hb?MCV?MCH?MCHC?RDW), hemoglobin electrophoresis and erythrocyte osmotic fragility analyses were done in all of samples for each phenotypic thalassemia in the childbearing age. These were successfully done by automatic blood corpuscle F820 analyzer System (CIS Co, Japan ). Rapid Electrophoresis system (Helena Laboratories, U.S.A) and erythrocyte osmotic fragility experiment"a tube method" respectively.
     方法 用日本CIS公司F82 0型血球分析系统、美国Helena公司SPIFE快速自动电泳分析系统及中山医科大红细胞渗透性“一管法”实验分别对经本室分子生物学技术诊断确诊的育龄成人不同基因类型的α地贫、β地贫及 β复合α地贫样品进行红细胞参数 (RBC、Hb、MCV、MCH、MCHC、RDW )、血红蛋白电泳及红细胞脆性分析。
短句来源
  相似匹配句对
     Rapid Determination of Lomefloxacin by Capillary Electrophoresis
     毛细管电泳法快速测定洛美沙星
短句来源
     Agarose Gel Electrophoresis for Rapid Screening of Rotavirus
     琼脂糖凝胶电泳法快速筛选轮状病毒
短句来源
     It is rapid, simple.
     本法出结果快、操作简便。
短句来源
     Rapid dacryocystorhinostomy.
     快速泪囊鼻腔吻合术
短句来源
     Electrophoresis of Modified Lignin
     改性木质素的电泳
短句来源
查询“rapid electrophoresis”译词为用户自定义的双语例句

    我想查看译文中含有:的双语例句
例句
为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法,我们为您准备了出自英文原文的大量英语例句,供您参考。
  rapid electrophoresis
Serum protein electrophoresis was performed with an automated rapid electrophoresis system.
      
Capillary electrophoresis has advanced enormously over the last 10 yr as a tool for DNA sequencing, driven by the human and other major genome projects and by the need for rapid electrophoresis-based DNA diagnostic tests.
      
The resolution of cleavage products by rapid electrophoresis through short denaturing polyacrylamide gels allows the analysis of large DNA fragments.
      


Total RNA of the immature Beijing wild soybean seeds was isolated by Lid precipitation method.Total mRNA was purified by oligo(dT)-cellulose affinity chromatography.The mRNA thus prepared showed enough translation activity when assayed in the rabbit reticulo-cyte cell-free system in vitro.Using the total mRNA as template and oligo(dT)12-18 as primer,the first strand and second strand cDNA were synthesized respectively by AMV reverse tran-scriptase and RNase H-DNA polymerase I.The length of the double stranded...

Total RNA of the immature Beijing wild soybean seeds was isolated by Lid precipitation method.Total mRNA was purified by oligo(dT)-cellulose affinity chromatography.The mRNA thus prepared showed enough translation activity when assayed in the rabbit reticulo-cyte cell-free system in vitro.Using the total mRNA as template and oligo(dT)12-18 as primer,the first strand and second strand cDNA were synthesized respectively by AMV reverse tran-scriptase and RNase H-DNA polymerase I.The length of the double stranded cDNA synthesized was about 200-5000 bp.The ds-cDNA with perfect plum ends was inserted into the Smal site of pUC19 by blunt end ligation.The resultant recombinant molecules were used to transform E.coli strain JM 107,and more than 800 white clones were obtained.Rapid electrophoresis and EcoRI-HindIII digestion analysis showed that most of the recombinant plasmids from the white clones contained insertions with various lengths and 3 out of 4 tested were about 1700,2600 and 1400 bp in length respectively.

用氯化锂沉淀法从野生大豆(G.soja)未成熟种子中制备总RNA,经oligo(dT)-纤维素柱亲和层析,获得总mRNA,在兔网织红细胞体系中表现出一定翻译活性。以总mRNA为模板,oligo(dT)_(12)(?)为引物,反转录酶催化合成第一链cDNA,RNase H-DNA聚合酶Ⅰ协同合成第二链cDNA。双链cDNA的长度大约为200—5000 bp,且不存在发夹结构。将双链cDNA修补后钝端连接到pUC 19质粒的Sma 1位点,转化E.coli JM107,获得800多个白色重组子克隆。快速电泳检测及酶切分析表明,多数重组子带有插入片段,其中3个重组子的插入片段长度大致为1700 bp、2600 bp和1400 bp。

TuMV particles were purified from artificially infected leaves of Brasslca juneaen, and TuMV-RNA was extracted from these particles. The virus thus purified showed typical nucleo-protein absorbent peak under UV light scanning, and the ratio of A260/A280 was 1.21. The RNA thus prepared showed typical ribonucleic acid absorbent peak, and the ratio of A260/A230 was 2.2. The RNA was then used as template and oligo (dT) 12-18 together with the end product of DNase digested calf thymus DNA were used as primer for...

TuMV particles were purified from artificially infected leaves of Brasslca juneaen, and TuMV-RNA was extracted from these particles. The virus thus purified showed typical nucleo-protein absorbent peak under UV light scanning, and the ratio of A260/A280 was 1.21. The RNA thus prepared showed typical ribonucleic acid absorbent peak, and the ratio of A260/A230 was 2.2. The RNA was then used as template and oligo (dT) 12-18 together with the end product of DNase digested calf thymus DNA were used as primer for the synthesis of cDNA. The length of double stranded cDNAs synthesized were distributed continuously with the sizes between 500-4300bp. The ds-cDNA repaired by Klenow fragment was inserted into the Smal site of pUC19 by blunt-end ligation. Then the recombinant molecules were used to transform E. coli strain DH5α. Rapid electrophoresis of plasmid prepared by alkali method, EcoRI-HindⅢ digestion and in situ hybridization with32P labeled TuMV-RNA showed ihat inserted fragments were with various sizes and complement to TuMV-RNA.

从接种芜菁花叶病毒后发病芥菜(Brassica Juneaen)中提取病毒,然后提取其核酸(TuMV-RNA)。以纯化的TuMV-RNA为模板,以Oligo(dT)_(12-18)及小牛胸腺DNA水解物为引物,合成双链cDNA,双链cDNA长度约500—4300bp。将双链cDNA补齐后,钝端连接到pUC19质粒的Smal位点,转化E.coli DH,获得500多个白色克隆。菌落原位杂交及酶切分析表明,重组质粒中的插入片段大多数为芜菁花叶病毒RNA的互补DNA,长度为500—4000bp。

Genomic DNA extracted from beef samples was amplified by the poly-merase chain reaction for identification of beef.The sequence selected for amplificationconsisted of 218 bp lying in the 1.709 satellite DNA of bovine,a pair of syntheticoligonucleotides flanking this sequence were used as primers. The amplified productswere subjected to rapid electrophoresis in an agarose gel and visualized under ultravioletillumination after EB staining. A Hae Ⅲ restriction endonuclease test was simultaneous-ly made for...

Genomic DNA extracted from beef samples was amplified by the poly-merase chain reaction for identification of beef.The sequence selected for amplificationconsisted of 218 bp lying in the 1.709 satellite DNA of bovine,a pair of syntheticoligonucleotides flanking this sequence were used as primers. The amplified productswere subjected to rapid electrophoresis in an agarose gel and visualized under ultravioletillumination after EB staining. A Hae Ⅲ restriction endonuclease test was simultaneous-ly made for verifying the specificity of the PCR amplification. The results showed that aspecific amplification fragment of the expected size was detected and demonstrated thatit was positive for bovine,cow,buffalo and yak meat,but negative for equine,ovine,goat,camel,deer,swine and mouse meat etc. An amount as small as 33.6 fg totalDNA from raw beef samples and 0.324 pg DNA from cooked or autoclaved beef samples could be detected by PCR.

依据牛1.709卫星DNA序列设计了1对引物,建立了PCR鉴定生、熟牛肉的方法。应用本方法特异地扩增出预期的牛218bpDNA片段,其检测敏感度对生牛肉达33.6fgDNA,对熟牛肉和高压牛肉为0.32pg。运用该引物均可扩增出水牛、牦牛、奶牛、黄牛肉单一的相同大小的DNA条带,而对马、山羊、绵羊、骆驼、鹿、猪等15种动物肉的DNA扩增则呈阴性。扩增片段经HaeⅢ酶切分析确认,所得129、79、10bp片段与微机分析结果一致。利用本法对103份生、熟牛肉及其制品进行鉴定,检出率为100%。对各种样品检测,均可在6h内完成。

 
<< 更多相关文摘    
图标索引 相关查询

 


 
CNKI小工具
在英文学术搜索中查有关rapid electrophoresis的内容
在知识搜索中查有关rapid electrophoresis的内容
在数字搜索中查有关rapid electrophoresis的内容
在概念知识元中查有关rapid electrophoresis的内容
在学术趋势中查有关rapid electrophoresis的内容
 
 

CNKI主页设CNKI翻译助手为主页 | 收藏CNKI翻译助手 | 广告服务 | 英文学术搜索
版权图标  2008 CNKI-中国知网
京ICP证040431号 互联网出版许可证 新出网证(京)字008号
北京市公安局海淀分局 备案号:110 1081725
版权图标 2008中国知网(cnki) 中国学术期刊(光盘版)电子杂志社