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reference standard
相关语句
  参考标准
    Objective To study the determination of biological activity of α_1-AT with chromogenic substrate and its influential factors using fresh pooled normal human plasma as reference standard.
    目的研究用发色底物法测定α1-AT生物活性时,以正常人混合血浆为参考标准的血浆稀释度范围和测定的影响因素。
短句来源
    Conclusion:Disc height ≤3 mm may be used as reference standard for the diagnosis of the foraminal stenosis in lower lumbar spine.
    结论下腰椎间盘高度≤3mm可以作为椎间孔狭窄诊断的参考标准
短句来源
    The activity of t-PA was measured by the fibrin plate method as compared to the urokinase reference standard.
    t-PA活性测定以尿激酶(UK)为参考标准
短句来源
    Objective To determine the existence of LipL41 gene in 15 Chinese reference standard strains belonging to 15 serogroups of Leptospira interrogans and 2 international standard strains belonging to 2 serogroups of Leptospira biflexa for understanding physical expression of the gene in the strains, construct prokaryotic expression system of the gene and identify immunity of the expression products.
    目的 确定我国 15群 15株问号钩端螺旋体 (简称钩体 )参考标准株和 2群 2株双曲钩体国际标准株携带LipL4 1基因情况 ,构建该基因的原核表达系统 ,鉴定表达产物的免疫原性。
短句来源
  参考标准
    Objective To study the determination of biological activity of α_1-AT with chromogenic substrate and its influential factors using fresh pooled normal human plasma as reference standard.
    目的研究用发色底物法测定α1-AT生物活性时,以正常人混合血浆为参考标准的血浆稀释度范围和测定的影响因素。
短句来源
    Conclusion:Disc height ≤3 mm may be used as reference standard for the diagnosis of the foraminal stenosis in lower lumbar spine.
    结论下腰椎间盘高度≤3mm可以作为椎间孔狭窄诊断的参考标准
短句来源
    The activity of t-PA was measured by the fibrin plate method as compared to the urokinase reference standard.
    t-PA活性测定以尿激酶(UK)为参考标准
短句来源
    Objective To determine the existence of LipL41 gene in 15 Chinese reference standard strains belonging to 15 serogroups of Leptospira interrogans and 2 international standard strains belonging to 2 serogroups of Leptospira biflexa for understanding physical expression of the gene in the strains, construct prokaryotic expression system of the gene and identify immunity of the expression products.
    目的 确定我国 15群 15株问号钩端螺旋体 (简称钩体 )参考标准株和 2群 2株双曲钩体国际标准株携带LipL4 1基因情况 ,构建该基因的原核表达系统 ,鉴定表达产物的免疫原性。
短句来源
  “reference standard”译为未确定词的双语例句
    According to the cDNA sequences of IRP2 and TfR in NCBI Gene Bank,the primers were designed and β-actin was used for internal competitive reference standard,and then RT-PCR was performed.
    按照 5′端相同 ,3′端互补的原则 ,根据NCBIGeneBank中IRPs 2和TfR蛋白受体的cDNA序列设计引物 ,以 β actin为内参对照 ,做RT PCR。
短句来源
    Method Total mRNA was extracted from both the sample of mouse normal liver tissue and subcutaneously transplanted hepatic carcinoma (H_ 22 cells) with Trizol method, then cDNA was retro-transcripted (RT). β-actin was used as internal reference standard. H-2 (at 387bp) and β-actin (at 550bp) were amplified simultaneously with multiple PCR.
    方法:各组织标本均采用Trizol法提取mRNA,引入细胞管家基因β-actin作为内参照物,通过RT法获取cDNA,多重PCR法同时扩增387bp的H-2抗原和550bp的β-actin。
短句来源
    Conclusion The experiment proved thatα1-AT biological activity was determined using fresh pooled human plasma as reference standard,the method is stable and reliable. Except sodium citrate,all of the materials used in the assay did not influence the determination ofα1-AT activity.
    结论以正常人混合血浆为参考标准品,用酶标仪测定α1-AT的生物活性,快速、准确,方法稳定可靠,α1-AT制备过程中常用的几种物质,除枸橼酸钠外,对其活性测定均无影响。
短句来源
    Studies on Standardization of the First National Reference Standard for Recombinant DNA-derived Human Growth Hormone(rhGH)
    首次DNA重组人生长激素(rhGH)国家标准品的研制
短句来源
    For defining the quality of experimental animals and offering a reference standard for choice the experimental animals, the latent Pneumocystis infection in rat , mice and rabbit were investigated. To compare the latent infection rate among different kinds of animals.
    本文用病原学和PCR方法对普通级实验动物肺孢子菌隐性感染情况进行了调查,对比了不同品系普通级实验动物肺孢子菌隐性感染率,并且对病原学染色法和PCR方法在检测肺孢子菌隐性感染的敏感性进行了探讨。
短句来源
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  reference standard
The described variant of the indentation method requires the preliminary calibration against a reference (standard) glass; i.e., it is a relative method.
      
The obtained values of the parameters can be used in practice as reference standard.
      
The procedure accuracy was evaluated by determining germanium in a reference standard sample of steel after preliminary separation by solvent extraction.
      
IUPAC Recommendations for Reference Standard Samples in pH Measurements
      
WPC 80, sodium caseinate and their hydrolysates were high-quality proteins and had a surplus of essential amino acids compared with the FAO/WHO/UNU (1985) reference standard.
      
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Porcine tissue plasminogen activator (t-PA) was extracted from delipidized heart tissue with pH 4.2, 0.45 mol/L potassium acetate buffer. The t-P A in the crude fraction prepared from the acid tissue extract by precipitation with ammonium sulphate was adsorbed on a fibrin-sepharose column and then eluted from the column with 2M KSCN. The t-PA was finally purified by Sephadex G-150 gel filtration. The final product has a specific activity of 39 038.5 IU/mg and a molecular weight of 67 000 as measured by SDS-polyacrylamide...

Porcine tissue plasminogen activator (t-PA) was extracted from delipidized heart tissue with pH 4.2, 0.45 mol/L potassium acetate buffer. The t-P A in the crude fraction prepared from the acid tissue extract by precipitation with ammonium sulphate was adsorbed on a fibrin-sepharose column and then eluted from the column with 2M KSCN. The t-PA was finally purified by Sephadex G-150 gel filtration. The final product has a specific activity of 39 038.5 IU/mg and a molecular weight of 67 000 as measured by SDS-polyacrylamide gel electrophoresis. The application of the protease inhibitor aprotinin and 6-aminohexanoio acid in the purification procedure abolished the conversion of one-chain t-PA to two-chain t-PA. The activity of t-PA was measured by the fibrin plate method as compared to the urokinase reference standard.

取猪心组织用丙酮去脂,pH4.2、0.45mol/L醋酸钾缓冲液抽提组织型纤溶酶原激活剂(t-PA)。抽提液经硫酸铵分段盐析,然后用纤维蛋白-Sepharose柱吸附t-PA,2mol/L硫氰酸钾洗脱的t-PA溶液经Sephadex G-150凝胶过滤,得到纯的t-PA。其比活性为39038.5 IU/mg,SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)鉴定其分子量为67000。提纯过程中应用了蛋白酶抑制剂(aprotinin)以及6-氨基己酸,以抑制单链t-PA转变为双链t-PA。t-PA活性测定以尿激酶(UK)为参考标准。

The strain P3 of inactivated Japanese encephalitis virus cultured in Vero cells was concentrated by ultrafiltration and treated by protamine sulfate, then ultracentrifuged with 40%sucrose bed at 27500rpm for 6 hours. The precipitate was collected and used for the preparation of JE vaccine. The protien content of the JE vaccine was 0.012~0. 035 mg/ml, and the vaccine titer after 8-folds dilution was stiIl higher than that of the reference standards of Japanese and Chinese JE vaccines. The residual DNA and...

The strain P3 of inactivated Japanese encephalitis virus cultured in Vero cells was concentrated by ultrafiltration and treated by protamine sulfate, then ultracentrifuged with 40%sucrose bed at 27500rpm for 6 hours. The precipitate was collected and used for the preparation of JE vaccine. The protien content of the JE vaccine was 0.012~0. 035 mg/ml, and the vaccine titer after 8-folds dilution was stiIl higher than that of the reference standards of Japanese and Chinese JE vaccines. The residual DNA and calf serum in vaccine were <100pg/0. 5ml and <1μg/ml, respectively.

经Vero细胞培养的P3株乙脑灭活病毒,以超滤浓缩和硫酸色精蛋白处理后,40%蔗糖垫层在27500r/min离心6小时,收集沉淀制成提纯乙脑疫苗。其蛋白含量在0.012~0.035mg/ml,稀释8倍后其疫苗效价仍能超过日本提纯鼠脑乙脑疫苗参考标准和我国乙脑疫苗参考标准。Vero细胞残余DNA<100pg/0.5ml,残余牛血清<1μg/ml。

A new method to prepare proline thiohydantoin which is required as reference standard for development of C terminal sequencing is reported.Proline thiohydantoin was prepared using a straightforward method involving reaction of proline with trimethylsilylisothiocyanate(TMS ITC).The product was characterized by amino acid analysis,UV spectrum,mass spectrometry and NMR.Different reaction conditions were investigated and the chemical mechanism scheme of proline thiohydantoin is presented.The yield of proline...

A new method to prepare proline thiohydantoin which is required as reference standard for development of C terminal sequencing is reported.Proline thiohydantoin was prepared using a straightforward method involving reaction of proline with trimethylsilylisothiocyanate(TMS ITC).The product was characterized by amino acid analysis,UV spectrum,mass spectrometry and NMR.Different reaction conditions were investigated and the chemical mechanism scheme of proline thiohydantoin is presented.The yield of proline thiohydantoin reached up to 96%.

采用L-型脯氨酸(L-Pro)作为原料,三甲基硅烷异硫氰酸酯(TMS-ITC)作为偶联试剂制备脯氨酸乙内酰硫脲(TH-Pro).产物经反相HPLC分离纯化,并通过氨基酸组成分析,紫外光谱扫描,质谱和核磁共振等方法鉴定.反应产率高达96%.

 
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