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plant lectin genes
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  “plant lectin genes”译为未确定词的双语例句
     The sequence from Wan82-178 show that the cloned fragment is 999bp. Like most plant lectin genes, there is no intron in the amplified sequence.
     根据大豆凝集素基因设计特异性引物,从大豆材料皖82-178(抗)和通山薄皮黄豆甲(感)中获得凝集素基因。
短句来源
     The sequence data showed that the cloned fragment is 781 bp. Like most plant lectin genes,there is no intron in the amplified sequence. The open reading frame of the fragment codes a 23 kD polypeptide which contains 227 amino acid residues and there is an signalsequence with 28 amino acid residues at its NH 2 terminus.
     序列分析表明,克隆到的基因片段大小为781bp,没有内含子,编码1条长227个氨基酸、分子量约23kD的肽链,其中N-端28个氨基酸是信号肽。
短句来源
     Like most plant lectin genes, there is no intron in the amplified sequence. The open reading frame of the fragment codes a 23kD polypeptide which contains 227 amino acid residues and there is a signal sequence with 28 amino acid residues at its NH 2 terminus.
     序列分析表明 ,克隆到的基因片段大小为 781bp,没有内含子 ,编码一条长 2 2 7个氨基酸、分子量约 2 3k D的肽链 ,其中 N-端 2 8个氨基酸是信号肽。
短句来源
  相似匹配句对
     THE PHYSIOLOGICAL ROLE OF PLANT LECTIN IN PLANT
     植物凝集素在植物体内的生理作用
短句来源
     Study on the Molecular Biology of Plant Lectin
     植物凝集素的分子生物学研究
短句来源
     PLANT POLYPHENOLS
     植物多酚(英文)
短句来源
     Plant Phototropism
     植物的向光性
短句来源
     SYSTEMATIC INVESTIGATION ON,PLANT GENE POL YMORPHISM(Ⅱ)MOLECULAR EVOLUTION OF LECTIN GENES
     植物基因多态性的系统分类学研究(Ⅱ)凝集素基因的分子进化
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By the aid of Polymerase Chain Reaction,the sequence coding for rice germ lectin (RGL) was isolated and cloned into the Sma Ⅰ site of pBluescript SK (+).The sequence data showed that the cloned fragment is 781 bp.Like most plant lectin genes,there is no intron in the amplified sequence.The open reading frame of the fragment codes a 23 kD polypeptide which contains 227 amino acid residues and there is an signalsequence with 28 amino acid residues at its NH 2 terminus.The amplified fragment is highly...

By the aid of Polymerase Chain Reaction,the sequence coding for rice germ lectin (RGL) was isolated and cloned into the Sma Ⅰ site of pBluescript SK (+).The sequence data showed that the cloned fragment is 781 bp.Like most plant lectin genes,there is no intron in the amplified sequence.The open reading frame of the fragment codes a 23 kD polypeptide which contains 227 amino acid residues and there is an signalsequence with 28 amino acid residues at its NH 2 terminus.The amplified fragment is highly homologous to the published cDNA sequence with similarity of 99.74%,having two base changes at 167 and 563 but without changing amino acid.The fragment was then expressed in E.coli strain BL21 (DE3) by inserting it into the expression vetor pRSET C.The expressed protein account for about 5% of the total soluble proteins.The establishment of this expression system provides an ideal model for further studies of its biological activities in vitro ,especially its defensive functions to pests and diseases and could be taken as a useful gene for plant genetic engineering.

以水稻基因组DNA为模板,以特异引物经聚合酶链式反应方法扩增出稻胚凝集素基因并克隆到E.coli质粒pBluescriptSK(+)的SmaⅠ位点。序列分析表明,克隆到的基因片段大小为781bp,没有内含子,编码1条长227个氨基酸、分子量约23kD的肽链,其中N-端28个氨基酸是信号肽。与报道的稻胚凝集素cDNA序列进行顺序同源性比较,发现它们之间有很高的同源性(99.74%),其编码区第167和563位各有1个碱基突变,但两者均为同义突变,并未造成氨基酸序列的变化。回收插入片段克隆到表达载体pRSET-C中,在大肠杆菌BL21(DE3)中得到表达,SDS-PAGE电泳图谱扫描结果表明,融合蛋白表达量约占菌体总蛋白的5%。鉴于凝集素可能具有抗病虫害的能力,稻胚凝集素基因的克隆及其在原核系统中的成功表达有助于我们进一步揭示稻胚凝集素在植物防御以及其他生理活动中的功能,并为植物抗病虫基因工程提供有用的素材

By the aid of polymerase chain reaction, the sequence coding for lectin from common wild rice was isolated and cloned into the SmaI site of pBluescript SK(+).The sequence data showed that the cloned fragment is 781bp. Like most plant lectin genes, there is no intron in the amplified sequence. The open reading frame of the fragment codes a 23kD polypeptide which contains 227 amino acid residues and there is a signal sequence with 28 amino acid residues at its NH 2 terminus.The amplified fragment is highly...

By the aid of polymerase chain reaction, the sequence coding for lectin from common wild rice was isolated and cloned into the SmaI site of pBluescript SK(+).The sequence data showed that the cloned fragment is 781bp. Like most plant lectin genes, there is no intron in the amplified sequence. The open reading frame of the fragment codes a 23kD polypeptide which contains 227 amino acid residues and there is a signal sequence with 28 amino acid residues at its NH 2 terminus.The amplified fragment is highly homologous to the overseas published cDNA sequence with an identity of 99.48%, having three base changes at 163,189 and 585 but without changing amino acids. The establishment of this expression system provides an ideal model for further studies of its biological activities in vitro, especially its defensive functions to pests and diseases, and could provide a useful material for plant genetic engineering.

以我国普通野生稻基因组 DNA为模板 ,以特异引物经聚合酶链式反应方法扩增出凝集素基因并克隆到 E.coli质粒 p Bluescript SK( +)的 Sma I位点。序列分析表明 ,克隆到的基因片段大小为 781bp,没有内含子 ,编码一条长 2 2 7个氨基酸、分子量约 2 3k D的肽链 ,其中 N-端 2 8个氨基酸是信号肽。与报道的栽培稻凝集素 c DNA序列进行顺序同源性比较 ,发现他们之间有很高的同源性 ( 99.48% ) ,其非编码区第 32位碱基缺失 ,编码区第 163、189和 5 85位各有一个碱基突变 ,但三者均为同义突变 ,并未造成氨基酸序列的变化。鉴于凝集素可能具有抗病、虫害的能力 ,普通野生稻凝集素基因的克隆及序列分析有助于进一步揭示水稻凝集素在植物防御以及其它生理活动中的功能 ,并为植物抗病虫基因工程提供有用的素材

In this paper,the progress of genetically modified organism crops (GMO) was firstly introduced in brief,then recent advancement of research on anti insect GMO were reviewed,especially for GMO with B.t. toxins genes,proteinase inhibitor genes,plant lectin genes,and other anti insect genes.In addition,the phenomenon of gene silencing in anti insect GMO was also discussed.Lastly,the prospect of anti insect GMO development was foreseen.

简要介绍了转基因作物 (GMO)的发展概况 ,对近年来国内外抗虫 GMO在转 B.t.毒素蛋白GMO;转蛋白酶抑制剂 GMO;转植物凝集素 GMO;其他类型抗虫 GMO;抗虫的基因沉默与失活现象等 5个方面的研究进展进行了论述。最后 ,对抗虫 GMO的发展前景进行了展望。

 
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