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The upper 8 centimeters of the great saphenous vein were studied on 102 sides (84male, 18 female) of adult Chinese cadavers. The chief results were as follows: 1. The surface projection of the sapheno-femoral junction is variable. It lies at avariable distance below the point which is situated opposite the junction of the lateraltwo thirds and medial third of the line joining the anterior superior iliac spine to thepubic tubercle. The mean value of the distance between this point and the sapheno-femoral junction...

The upper 8 centimeters of the great saphenous vein were studied on 102 sides (84male, 18 female) of adult Chinese cadavers. The chief results were as follows: 1. The surface projection of the sapheno-femoral junction is variable. It lies at avariable distance below the point which is situated opposite the junction of the lateraltwo thirds and medial third of the line joining the anterior superior iliac spine to thepubic tubercle. The mean value of the distance between this point and the sapheno-femoral junction is 3.84±0.07 cm (min. 2.1 cm, max. 5.5 cm). 2. Duplication of the great saphenous vein is rare. It is found on 6 sides only(5.88±2.33%) in our material. 3. There is a close relation between the terminal part of the great saphenous veinand the superficial external pudendal artery. In 65.63±4.85% of the cases, this arterypasses behind the terminal part of the vein. 4. The upper part of the great saphenous vein is joined by lateral, medial, or bothaccessory saphenous veins in 86 sides (84.31±3.60%), among which the lateral accessorysaphenous vein occurs most frequently (61.61±5.24%). 5. The junction between the deep external pudendal vein and the great saphenousvein lies within the fossa ovalies in 25 sides (24.51±4.26%). 6. The patterns of the tributaries of the great saphenous vein may be classifiedinto 4 types and 11 subtypes, according to the number of veins and the manner of theircombination. Type II_2, in which two tributaries are combined, occurs more often thanany other types (38.23±4.81%). In the point of view of practical application, the pat-terns of the tributaries may be classified into different types, according to the numberof the direct opening on the upper part of the great saphenous vein, among which the3 tributary and 4 tributary types are the highest in percentage, being 34.31±4.70% and33.33±4.67% respectively. 7. The position of the tributaries which open into the upper part of the greatsaphenous vein is variable. In the majority of sides (91.18±2.80%), the position lieswithin the uppermost 4 centimeters of the great saphenous vein (min. 0.3 cm, max.7.2 cm). The surgical significance is briefly discussed.

大隐静脉上段的形态特点对大隐静脉高位结扎手术具有重要的意义。本文观察了52具(102例)不同性别成年下肢,得到下列主要结果: 1.大隐静脉全部经卵圆窝汇入股静脉,根据它汇入股静脉的部位不同,可把它分为三个类型,其中第Ⅲ型最多占61.76±4.81%。 2.隐股结合点的体表投影是在经髂前上棘与耻骨结节连线外侧2/3与内侧1/3交界点下方平均为3.89±0.07厘米、隐胶结合点的内外位置变化较小。 3.绝大多数个体(95.96±1.95%)的阴部外浅动脉与大隐静脉上端发生紧密的紧邻关系,其中约半数(65.63±4.85%)经静脉上端的后方。 4.双隐静脉是一种较少见的变异,我们仅观察到6例(5.88±2.33%)。 5.大隐静脉上段除接纳三个浅属支外并通常也接纳内、外侧副隐静脉(84.31±3.60%),其中外侧副隐静脉出现率是61.63±5.24%,内侧副隐静脉出现率为16.28±3.98%,内、外侧副隐静脉同时出现率为22.09±4.47%。 6.在观察的全部材料中有24.51±4.26%个体的阴部外深静脉任卵圆窝内汇入大隐静脉末端的内侧面,因此易为手术者所忽略。 7.大隐静脉上段属支根据参加联合的数目和联合...

大隐静脉上段的形态特点对大隐静脉高位结扎手术具有重要的意义。本文观察了52具(102例)不同性别成年下肢,得到下列主要结果: 1.大隐静脉全部经卵圆窝汇入股静脉,根据它汇入股静脉的部位不同,可把它分为三个类型,其中第Ⅲ型最多占61.76±4.81%。 2.隐股结合点的体表投影是在经髂前上棘与耻骨结节连线外侧2/3与内侧1/3交界点下方平均为3.89±0.07厘米、隐胶结合点的内外位置变化较小。 3.绝大多数个体(95.96±1.95%)的阴部外浅动脉与大隐静脉上端发生紧密的紧邻关系,其中约半数(65.63±4.85%)经静脉上端的后方。 4.双隐静脉是一种较少见的变异,我们仅观察到6例(5.88±2.33%)。 5.大隐静脉上段除接纳三个浅属支外并通常也接纳内、外侧副隐静脉(84.31±3.60%),其中外侧副隐静脉出现率是61.63±5.24%,内侧副隐静脉出现率为16.28±3.98%,内、外侧副隐静脉同时出现率为22.09±4.47%。 6.在观察的全部材料中有24.51±4.26%个体的阴部外深静脉任卵圆窝内汇入大隐静脉末端的内侧面,因此易为手术者所忽略。 7.大隐静脉上段属支根据参加联合的数目和联合的形式得出四个类型和11个分型,本材料中以第Ⅱ型最多,为总数的38.23±4.81%,其次是第Ⅰ型,占总数的29.41±4.51%。同时考虑实际应用根据注入属支数目来分型,其中以三支型和四支型最多,分别为总数的34.31±4.70%、33.33±4.67%,其次是二支型占24.51±4.26%,六支型最少占0.98±0.68%。 8.大隐静脉上段的绝大多数属支(91.18±2.80%)是在隐股结合点下方4厘米以内汇入大隐静脉。本文曾在湖南省医药卫生学会1962年年会解剖学分会上宣读,并加以补充和修改。

In order to determine the extent and degree of chloroquine resistance of falciparum malaria in our country, we have prepared both the drug-coated microplates and freeze-dried medium for the in vitro microtechnique. Each microplate has 40 wells, four horizontal rows with 10 wells each, wells 1 and 10 were untreated to serve as controls and wells 2-9 coated with 1.0, 2.0, 4.0, 5.7, 8.0, 16.0, 32.0 and 64.0 pmol of chloroquine diphosphate. The freeze-dried medium contained RPMI 1640, HEPES buffer and 15% type AB...

In order to determine the extent and degree of chloroquine resistance of falciparum malaria in our country, we have prepared both the drug-coated microplates and freeze-dried medium for the in vitro microtechnique. Each microplate has 40 wells, four horizontal rows with 10 wells each, wells 1 and 10 were untreated to serve as controls and wells 2-9 coated with 1.0, 2.0, 4.0, 5.7, 8.0, 16.0, 32.0 and 64.0 pmol of chloroquine diphosphate. The freeze-dried medium contained RPMI 1640, HEPES buffer and 15% type AB human serum. Prior to use, it was stored under refrigeration. 0.9ml of 0.21% sodium bicarbonate solution was injected into a vial with freeze-dried medium and the latter dissolved by shaking, and then 0.1ml of patient's blood added, from which an aliquot of 50μl of the mixture being placed in each well. P. falciparum kept in continuous culture for more than a year was used for laboratory assessment of reliability of the freeze-dried medium and microplates. The results obtained showed that the freeze-dried medium stored for one year at 4℃ could still support the growth of malaria parasites while those at 36℃ for 5 days and at 26℃ for 10 days failed. Field investigation was carried out in some parts of Hainan Island, South Yunnan, west Guangxi, South Guizhou and East Anhui. The results showed that the home-made plates were equally effective as those provided by WHO. The application of the freeze-dried medium in the assessment of drug-resistant, malaria furnished the test with a good readability and proved to be suitable for field use. It is clear that the home-made freeze-dried medium might be an important tool in the surveillance of chloroquine resistant malaria.(This investigation received the financial support of the UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases, SWG on the Chemotherapy of Malaria, Project ID No:800214)

体外微量法测定恶性疟原虫对氯喹的敏感性,具有简便、快速和准确等优点,但要有预制的涂氯喳板及培养基才能用于现场调查。世界卫生组织自1979年起试制这种板和冰冻干燥培养基,但后者至今尚无供应。 为了尽快摸清我国抗氯喹恶性疟的分布情况,我们于1979年底仿制涂氯喹板获得初步成功后,1980年又着手研制冻干培养基,几经改进,制成的冻干培养基在4℃保存下有效期已达1年以上,可在加液后立即使用,不需过滤灭菌等手续,所制涂氯喹板的药效曲线与世界卫生组织的完全一致。且增加了一个对照井及一个药物剂量,有利于预先观察疟原虫生长情况及测定较高抗性的病例。1981年7~10月间经粤、桂、滇、黔、皖等省区试用,效果满意。

Immunomicrospheres prepared by covalent binding of sheep anti-rabbit IgG to car-boxylated polystyrene latex was used as solid phase second antibody in radioimmunoassay (RIA). It was compared with another solid phase second antibody "Immunosorbent" in the determination of LH, FSH and PEL. "Immunosorbent" and classical liquid phase double antibody RIA Kits for LH, FSH and PRL were supplied by WHO.It was found that in all these determinations the results obtained by solid phase methods and liquid phase method were...

Immunomicrospheres prepared by covalent binding of sheep anti-rabbit IgG to car-boxylated polystyrene latex was used as solid phase second antibody in radioimmunoassay (RIA). It was compared with another solid phase second antibody "Immunosorbent" in the determination of LH, FSH and PEL. "Immunosorbent" and classical liquid phase double antibody RIA Kits for LH, FSH and PRL were supplied by WHO.It was found that in all these determinations the results obtained by solid phase methods and liquid phase method were closely correlated, the coefficients of correlation were 0.97-0.99. The assays were more simple and precise by using solid phase second antibody, and Immunomicrospere was even better than "Immunosorbent" in some respects.

共价交联羊抗兔IgG到羧化聚苯乙烯胶乳上而制得的免疫微球,在放射免疫测定中被用作固相第二抗体。在 LH、FSH和PRL的测定中将免疫微球和另一个固相第二抗体“Immunosorbent”作了比较。“Immunosorbent”和LH、FSH、PRL放免液相双抗体法测定药盒均由世界卫生组织供应。在所有这些测定中,用固相法和液相法所获得的结果呈密切相关,相关系数在0.97~0.99之间。使用固相第二抗体可使测定更为精确、简便,而免疫微球在某些方面的效果比“Immunosorbent”更好些。

 
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