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the condition optimizes
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The present investigation describes an in vitro system detecting specific antibody forming cells from human peripheral bloodPeripheral nonT lymphocytes from 23 healthy volunteers were primed with sheep red blood cells(SRBC) in the presence of PWM for 5 days before a modified PFC assay was conductedThe condition optimized in the study confirmed the specificity in this system and a better result can be easily achieved than the conventional method without complicated procedureThe system may be clinically practicable and can be used for the research in human suject...

The present investigation describes an in vitro system detecting specific antibody forming cells from human peripheral bloodPeripheral nonT lymphocytes from 23 healthy volunteers were primed with sheep red blood cells(SRBC) in the presence of PWM for 5 days before a modified PFC assay was conductedThe condition optimized in the study confirmed the specificity in this system and a better result can be easily achieved than the conventional method without complicated procedureThe system may be clinically practicable and can be used for the research in human suject

采用以绵羊红细胞(SRBC)为免疫原,两次诱导并伴有pwm作用的免疫培养方法,对23例健康人外周血淋巴细胞进行体外诱导,并以空斑细胞(plaque-formingcel,PFC)技术检测培养中产生的特异性抗体形成细胞(antibody-producingcel),结果表明:该条件下的实验组产生的PFC数最高,细胞活性好。以兔RBC代替SRBC的特异性溶血试验中,几乎无PFC产生,证实SRBC诱导PFC的特异性及本实验体外免疫培养方法的可行性。此外,本文采用的改良PFC单电子层的检测方法,操作简便,便于临床应用,可作为研究人体免疫细胞功能的手段之一。

Based on the gray system theory, a novel method of gray relationship martrix analysis for multi-index orthogonally designed experiments was developed. This paper give its algorithm, which was realized by a compiled program. The method was applied in the condition optimizing process of TNT red water's catalyzed oxidation. The results showed that this method is useful and convenient.

采用灰色关联矩阵分析理论,建立了多指标正交试验的灰色关联分析法,给出了其算法并编制了程序。将其应用到TNT红水处理工艺中催化氧化条件的优化中,取得了较好的应用效果。

 
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