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osteogenic markers
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  成骨标志蛋白
     Objective To explore the effects of rhBMP-2(recombinant human bone morphogenetic protein-2) poly- butylcyanoacrylate nanoparticles sustained release system on protein expression of osteogenic markers in bone mesenchymal stem cells(BMSCs).
     目的探讨rhBMP-2聚氰基丙烯酸正丁酯纳米微球缓释系统作用BMSCs后对成骨标志蛋白表达的影响。
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  “osteogenic markers”译为未确定词的双语例句
     Effects of rhBMP-2 polybutylcyanoacrylate nanoparticles on expression of osteogenic markers in cultured bone mesenchymal stem cells
     rhBMP-2聚氰基丙烯酸正丁酯纳米微球缓释系统对骨髓间充质干细胞影响的实验研究
短句来源
     These cells were cultured in the osteogenic medium for two weeks, and real-time RT-PCR was employed to detect the expressions of osteogenic markers before and after osteogenic differention. Von Kossa staining was performed to evaluate the mineral deposition after osteogenic differentiation for two weeks.
     体外向成骨诱导Flkl~+间充质干细胞两周,利用Real-time RT-PCR分别检测成骨诱导1周和2周时成骨指标,如碱性磷酸酶(alkaline phosphatase,ALP)、成骨蛋白(osteopontin,OPN)、骨钙蛋白(osteocalcin,OC)、Cbfal(Core-bindingfactors)/Runx2的表达,用Von-Kossa染色法测定成骨诱导2周后钙质沉积。
短句来源
     Osteogenic markers including alkaline phosphatase (ALP), collagen I and the expression of osteocalcin were detected.
     检测诱导后细胞成骨细胞标志物碱性磷酸酶、I型胶原和骨钙素的表达。
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  相似匹配句对
     were its chromosome markers.
     等为其标志染色体,未显示有完整Y染色体存在。
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     Osteogenic markers including alkaline phosphatase (ALP), collagen I and the expression of osteocalcin were detected.
     检测诱导后细胞成骨细胞标志物碱性磷酸酶、I型胶原和骨钙素的表达。
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     Discourse Markers and Metacognition
     话语标记语与元认知
短句来源
     The osteogenic differentiation markers were determined by para position Nitrobenzene phosphoric acid (pNPP) and Alizarin Red S staining.
     用对硝基苯磷酸(pNPP)法和茜素红S染色定量法对BMSCs分化为成骨细胞的标志物进行测定。
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     Osteogenic growth peptide and osteoporosis
     骨生长肽与骨质疏松症
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  osteogenic markers
Parallel in vitro experiments using goat and rat MSCs corroborated in vivo data by demonstrating the expression of chondrogenic and osteogenic markers by biochemical and mRNA analyses.
      
PCR analysis showed significant increases in the gene expression of the osteogenic markers Runx2, alkaline phosphatase, and osteopontin in the heparin sulfate group compared with controls.
      
Importantly, the released HS contributed to improved wound healing over a 3-month period as determined by microcomputed tomography (μCT) scanning, histology, histomorphometry, and PCR for osteogenic markers.
      
In murine C2C12 cell line, rhBMP-2 induced an increase in the transcription of Smads and of osteogenic markers Runx2/Cbfa1 and Osterix, measured by semi-quantitative RT-PCR.
      
Sections were stained histochemically with Goldner's Trichrome stain and immuno-histochemically using human-specific antibodies against known osteogenic markers.
      
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AIM: To explore the proliferative characteristics of umbilical cord blood(UCB) and bone marrow monocytes(MNC) in the course of cultivation in vitro, and the adherent cells were induced to differentiate into osteoblast like cells. METHODS: The bone marrow MNC were separated from UCB and divided into 3 groups according to the differences of the medium: the Mesencult TM serum free medium, CellGRO medium+100 mL/L fetal bovine blood serum and DMEM/F12 medium+100 mL/L FBS. The adherent cells were gained by cultivated...

AIM: To explore the proliferative characteristics of umbilical cord blood(UCB) and bone marrow monocytes(MNC) in the course of cultivation in vitro, and the adherent cells were induced to differentiate into osteoblast like cells. METHODS: The bone marrow MNC were separated from UCB and divided into 3 groups according to the differences of the medium: the Mesencult TM serum free medium, CellGRO medium+100 mL/L fetal bovine blood serum and DMEM/F12 medium+100 mL/L FBS. The adherent cells were gained by cultivated in vitro. The different proliferative patterns in different mediums were observed. Dexamethasone, sodium β glycerophosphate and ascorbic acid were added into the culture medium to induce osteogenic differentiation. Osteogenic markers including alkaline phosphatase (ALP), collagen I and the expression of osteocalcin were detected. RESULTS: More adherent cells could be obtained with fetal bovine serum contained medium from both UCB and bone marrow MNC. The form of bone marrow MNC adherent cells was uniform and showed the shape of fibroblast, which was not found in UCB adherent cells but mixed with amounts of round shape cells. The adherent cells acquired from bone marrow could be passaged 10 times with a good behavior of proliferation. The adherent cells acquired from UCB could be passaged at most twice with decreasing cell total number and fibroblast like cell number. After cultured in the osteogenic differentiation medium, ALP, collagen Ⅰand the expression of osteocalcin were all positive in the cells from UCB and bone marrow. CONCLUSION: Typical mesenchymal stem cells could be acquired from adult bone marrow and induced into osteoblast in vitro. Adherent cells could be obtained from UCB cultured in vitro, but no typical mesenchymal stem cell like cell could be found. However, after ostegenic differentiation induction, although the morphological characters not similar to osteoblast, the osteogenic markers were all positively detected.

目的:探讨脐血和骨髓单个核细胞(monocytes,MNC)体外培养过程中的生长特性及诱导贴壁细胞向成骨细胞分化。方法:分离脐血和骨髓MNC,按培养基不同分成3组:分别为MesencultTM无血清培养基。CellGRO培养基+100mL/L胎牛血清。DMEM/F12培养基+100mL/LFBS。体外培养以获得贴壁细胞。观察不同培养条件下细胞扩增的效果。获得的贴壁细胞培养基中加入地塞米松、抗坏血酸和β-甘油磷酸,诱导细胞向成骨细胞分化。检测诱导后细胞成骨细胞标志物碱性磷酸酶、I型胶原和骨钙素的表达。结果:脐血和骨髓MNC在含有血清的培养基中可以获得更多的贴壁细胞。骨髓MNC贴壁细胞形态均一,呈典型的成纤维细胞样,而脐血贴壁细胞未获得典型的成纤维细胞样细胞,混有大量的圆形细胞。骨髓中获得的细胞可以持续传代10代以上仍保持良好的增殖状态;脐血贴壁细胞最多可以传代2次,细胞总数和纤维样细胞均逐代减少。诱导后的脐血和骨髓细胞均呈碱性磷酸酶阳性,免疫组化染色显示I型胶原和骨钙素表达阳性。结论:骨髓MNC体外培养可以获得典型的间充质干细胞且可以持续多代扩增,向成骨细胞诱导后,形态和标志物检测都呈典型的成骨细胞样。脐血MN...

目的:探讨脐血和骨髓单个核细胞(monocytes,MNC)体外培养过程中的生长特性及诱导贴壁细胞向成骨细胞分化。方法:分离脐血和骨髓MNC,按培养基不同分成3组:分别为MesencultTM无血清培养基。CellGRO培养基+100mL/L胎牛血清。DMEM/F12培养基+100mL/LFBS。体外培养以获得贴壁细胞。观察不同培养条件下细胞扩增的效果。获得的贴壁细胞培养基中加入地塞米松、抗坏血酸和β-甘油磷酸,诱导细胞向成骨细胞分化。检测诱导后细胞成骨细胞标志物碱性磷酸酶、I型胶原和骨钙素的表达。结果:脐血和骨髓MNC在含有血清的培养基中可以获得更多的贴壁细胞。骨髓MNC贴壁细胞形态均一,呈典型的成纤维细胞样,而脐血贴壁细胞未获得典型的成纤维细胞样细胞,混有大量的圆形细胞。骨髓中获得的细胞可以持续传代10代以上仍保持良好的增殖状态;脐血贴壁细胞最多可以传代2次,细胞总数和纤维样细胞均逐代减少。诱导后的脐血和骨髓细胞均呈碱性磷酸酶阳性,免疫组化染色显示I型胶原和骨钙素表达阳性。结论:骨髓MNC体外培养可以获得典型的间充质干细胞且可以持续多代扩增,向成骨细胞诱导后,形态和标志物检测都呈典型的成骨细胞样。脐血MNC体外培养可获得贴壁细胞,但不呈间充质干细胞样,向成骨细胞诱导后,虽形态与成骨细胞不相似,但成骨细胞

Objective To explore the effects of rhBMP-2(recombinant human bone morphogenetic protein-2) poly- butylcyanoacrylate nanoparticles sustained release system on protein expression of osteogenic markers in bone mesenchymal stem cells(BMSCs). Methods Immunocytochemical staining method and image analysis techniques were used to observe the effect on expression of osteocalcin,bone sialoprotein, osteopontin and typeⅠcollagen in rat BMSCs by adding rhBMP-2 polybutylcyanoacrylate nanoparticles or rhBMP-2 only....

Objective To explore the effects of rhBMP-2(recombinant human bone morphogenetic protein-2) poly- butylcyanoacrylate nanoparticles sustained release system on protein expression of osteogenic markers in bone mesenchymal stem cells(BMSCs). Methods Immunocytochemical staining method and image analysis techniques were used to observe the effect on expression of osteocalcin,bone sialoprotein, osteopontin and typeⅠcollagen in rat BMSCs by adding rhBMP-2 polybutylcyanoacrylate nanoparticles or rhBMP-2 only. Results The effect of rhBMP-2 polybutylcyanoacrylate particles on the expression of osteocalcin,bone sialoprotein and typeⅠcollagen were much stronger than those of rhBMP-2. Conclusion The rhBMP-2 polybutylcyanoacrylate nanoparticle sustained release system has stronger effect on differentiating the BMSCs into osteoblasts than rhBMP-2.

目的探讨rhBMP-2聚氰基丙烯酸正丁酯纳米微球缓释系统作用BMSCs后对成骨标志蛋白表达的影响。方法采用免疫细胞化学染色及图像分析方法,观察rhBMP-2纳米微球作用后对细胞中骨钙素、骨涎蛋白及Ⅰ型胶原表达的影响,并与单纯rhBMP-2作用细胞后的结果进行比较。结果rhBMP-2纳米微球作用后细胞中骨钙素、骨涎蛋白及Ⅰ型胶原的表达均明显强于单纯rhBMP-2作用后的效果。结论rhBMP-2纳米微球缓释系统促进BMSCs向成骨方向分化的作用强于单纯rhBMP-2的作用。

ObjectiveTo explore environmental conditions under which bone marrow stromal cells could be induced into osteogenic phenotype.MethodsRat bone marrow stromal cells were isolated and proliferated in vitro, and the 3rd passage was divided into the group A (control group), group B (cells cultured in the medium containing dexamethasone, β-glycerol disodium phosphate salt hydrate, vitamin C and active form of vitamin D_3), and group C (on the bases of group B, the cells were cultured additionally with fracture hematoma...

ObjectiveTo explore environmental conditions under which bone marrow stromal cells could be induced into osteogenic phenotype.MethodsRat bone marrow stromal cells were isolated and proliferated in vitro, and the 3rd passage was divided into the group A (control group), group B (cells cultured in the medium containing dexamethasone, β-glycerol disodium phosphate salt hydrate, vitamin C and active form of vitamin D_3), and group C (on the bases of group B, the cells were cultured additionally with fracture hematoma extract). On the post-induction day 5, 8, and 11, the morphological changes were observed and the osteogenic markers such as alkaline phosphotase (ALP), collagen type Ⅰ (Col Ⅰ) and osteocalcin (OCN) were assayed with immunohistochemical staining, the calcification was manifested with von Kossa staining.ResultsIn the group A, no evident osteogenic effects had been observed. In the group B, on 5th day post-induction, some bone marrow stromal cells underwent a morphological change, and mild expression of ALP and Col Ⅰ was observed but with no calcification effect. On 8th day post-induction, the ratio of morphologically changed cells increased, and the expression of ALP and Col Ⅰ increased still with no evident calcification. On 11th day post-induction, anti-OCN staining was positive and the calcium nodes were showed by von Kossa staining. The phenotype changes in the group C were similar to group B, but were more evident.ConclusionBone marrow stromal cells can be induced into osteogenic phenotype in vitro with small molecular inducers. Fracture hematoma extract can enhance this effect thus might be used as an addictive in osteogeneration.

目的探讨骨髓基质细胞分化为成骨表型的诱导条件。方法体外扩增大鼠骨髓基质细胞,将第3代细胞分为A组(对照组)、B组(加入地塞米松、β-甘油磷酸钠、维生素C、1,25-二羟维生素D3的诱导组)和C组(在B组基础上加大鼠长骨骨折血肿浸出液诱导组),于诱导后第5、8、11天观察细胞形态变化,用免疫组织化学方法检测诱导细胞的成骨样细胞标志分子(碱性磷酸酶、Ⅰ型胶原、骨钙素),并用von Kossa法检测培养物钙化情况。结果诱导大鼠骨髓基质细胞经小分子诱导培养5d后,部分发生形态学变化,碱性磷酸酶和I型胶原呈弱阳性表达,无钙质沉积;诱导8d后,发生形态学变化的细胞增多,呈集落生长,碱性磷酸酶和I型胶原表达增强,钙质沉积仍不明显;11d后,骨钙素开始表达,且出现钙质沉积。C组细胞表型变化与B组类似,但发生变化的细胞和钙质沉积的数量更多。结论骨髓基质细胞在体外可以被小分子物质诱导为成骨表型;骨折血肿浸出液能够加强这一作用,可作为一种诱导成骨的添加成分。

 
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