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butanol extraction
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  正丁醇萃取
     Using big hole absorbing resin,the production of tea saponin from 120 mL extract was 10.98 g and tea saponin content was 91 7%,while by n butanol extraction of the same extract the production of tea saponin was 5 86 g and tea saponin content was 78 0%.
     结果表明 ,取等量茶皂素提取液 12 0mL ,大孔树脂法得茶皂素产量 10 98g ,茶皂素质量分数 91 7% ,正丁醇萃取法产量 5 86g ,茶皂素质量分数78.0 %
短句来源
     After butanol extraction and separation on the silica gel column, the purity of the product Rd is about 90% and the yield is 34%.
     经饱和正丁醇萃取,硅胶柱分离,酶解产物Rd纯度可达90%,得率为34%。
短句来源
     After butanol extraction and separation on the silica gel column, the purity of the product Rh2 is about 90% and the yield is 32%.
     经饱和正丁醇萃取,硅胶柱分离,酶解产物Rh_2纯度可达90%,得率为32%。 人参皂苷-β-葡萄糖苷酶能水解20(S)-Rg3和20(R)-Rg3分别生成20(S)-Rh_2和20(R)-Rh_2,水解速度大体相同。
短句来源
     The result showed that butanol was the most effective agent for active component recovery at pH3. From the butanol extraction a purified sample, S227-4, was isolated by silica gel column chromatography and thin-layer chromatography .
     对正丁醇萃取相经过两次硅胶柱层析及薄层层析分离得到具有抗耐药菌活性的纯化样品S227-4。
短句来源
     The butanol extraction is an effective method of isolation of swainsonine from the solution of Metarhizium anisopliae.
     正丁醇萃取法是从发酵液中提取苦马豆素的有效方法。
短句来源
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  正丁醇抽提
     Alkaline PhoPhatases (ALP) were extracted and purified from chicken skeleton, liver and intestine with butanol extraction and chromatography on DEAE-cellulose (DE-52) and concanavalin ASepharose 4B. Skeleton and liver tissues ALPs were separated with polyacrylamide gradient gel electrophoresis into two components, with molecular weights of 154000 and 353000(skeleton), 187000 and 353000(liver).
     用正丁醇抽提、DEAE纤维素(DE—52)和Con·A—Sepharase 4B柱层析等方法从鸡的骨、肝和肠组织中提取碱性磷酸酶(ALP)。 提纯倍数分别为41倍(骨)、391倍(肝)和130倍(肠)。
短句来源
     The intestinal alkaline phosphatases (I-ALP)and liver alkaline phosphatases (L-ALP) have been extracted from the rabbit and partially purified by butanol extraction and chromatography on a DEAE-cellulose column eluted with linear gradient of sodium chloride.
     作者应用正丁醇抽提和DEAE-纤维素离子交换、NaCl线性梯度洗脱方法从家兔肝脏和小肠粘膜组织中提取出肝型碱性磷酸酶(L-ALP)和小肠型碱性磷酸酶(I-ALP).
短句来源
     The alkaline phosphatase was purified from the female adults of Ericerus pela (Chavannes) through homogenation, n butanol extraction, ammonium sulfate fractionation and Sephadex G 150 column gel filtration. The purification attained to 16 83 folds and the specific activity was 136 65 U/mg.
     白蜡虫Ericeruspela雌成虫经匀浆 ,正丁醇抽提 ,硫酸铵分段盐析 ,SephadexG 150凝胶过滤等步骤 ,得到比活力为 136 65U mg蛋白酶制品。
短句来源
     Methods Human DAF was purified from human erythrocyte ghost by trypsin digestion, butanol extraction, sequential chromatography on DE32 and immunoaffinity chromatography.
     方法 经胰蛋白酶消化、正丁醇抽提及DE3 2离子交换色谱和抗体亲和层析等步骤从人红细胞膜影中分离纯化人红细胞膜DAF。
短句来源
     ObjectiveTo purify decay accelerating factor (DAF) wi th biological activity from human erythrocyte membraneMethodHu man DAF was purified from human erythrocyte ghost by trypsinization, followed by butanol extraction and sequential chromatography on DE32 and Sepharose 6B. The purity of purified protein and its reactivity with antibody to human DAF was inv estigated by SDS PAGE and Wes tern blotting.
     目的 获得纯化并具有生物学活性的人红细胞膜衰变加速因子。 方法 经胰蛋白酶消化、正丁醇抽提及DE32和Sepharose 6B色谱分离等步骤从人红细胞膜影中分离纯化人红细胞膜衰变加速因子 (DAF)。
短句来源
  “butanol extraction”译为未确定词的双语例句
     (3)Butanol extraction were(0.9~3.2)mg/per capsule.
     ③正丁酸浸出物含量为09~32m g/粒;
短句来源
     Study on the Chemical Constituents of the Butanol Extraction of Cyclocarya paliurus(Batal.) Iljinsk
     青钱柳正丁醇部位化学成分研究
短句来源
     Production of Liposomal Crude Butanol Extraction(CBE)-based Vaccine and Its Antitumor Effect in Vivo
     宫颈癌U_(14)细胞正丁醇提取物脂质体瘤苗的制备及其抑瘤效应观察
短句来源
     Cellular and Humoral Immune Responses to Cervix Carcinoma in Mice Induced by Liposomal Crude Butanol Extraction(CBE)-based Vaccine
     U_(14)-CBE脂质体瘤苗诱导的细胞和体液免疫应答
短句来源
     The crude butanol extraction of U14 (U14-CBE) were prepared by 2.5% 1-butanol, and negative liposomes made up of PC, PG and Chol (7:1:2,molar ratio) were producted. The liposomal vaccine encapsulating U14-CBE was prepared by a combination of the thin-film hydration method, ultrasonication and extrusion through polycarbonate filters with a pore size of 220nm.
     正丁醇法提取U14细胞表面抗原(CBE),以PC,PG,Chol制成阴离子脂质体,运用薄膜水合法和超声粉碎、过膜相结合的方法,将CBE包裹入阴离子脂质体制成CBE-脂质体瘤苗。
短句来源
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  butanol extraction
Low selectivity for lincomycin in butanol extraction process leads to relatively higher content of impurities.
      
B(α)P was the positive control for the in vitro metabolism, and two adduct enrichment procedures: nuclease P1 digestion and butanol extraction.
      
Enrichment using butanol extraction gave three adducts unique to Sencor-DNA.
      
It is anticipated that noncytolytic butanol extraction will continue to prove a powerful approach for the isolation and characterization of a variety of cell-surface antigens.
      
The peptide was purified by ammonium sulfate precipitation, butanol extraction, followed by cation-exchange chromatography and reversed-phase chromatography.
      
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This paper presented a procedure of n-butanol extraction and air-blowing to produce a asphallic pitch by residuum of Gudao Oil. The pitch obtained is very similar with natural rock asphalt in chemical compsition and physical properties.Trial production and coating test showed that it can be a substitute of natural rock asphalt to produce lacquer product. The technological conditions of solvent extraction, air-blowing and the data of comparative analyses of the product are given.

本文论述了以孤岛减压渣油为原料,用正丁醇溶剂抽提和氧化的方法,制得的石油硬沥青,在物理性质及化学组成上均与天然岩沥青相似,并经制漆和涂敷试验表明,完全可以代替昂贵的天然岩沥青制造烘漆。文中给出了抽提和氧化等工艺条件及产品分析比较数据。

Two different forms of alkaline phosphatase ( AKPⅡ & AKP Ⅱ) were isolated and purified from Sinonovacula constricta by n-butanol extraction, ammonium sulfate fractionation, DEAE-cel-lulose chromatography and gel. filtration with Sephadex-G-200. Their molecular weights were drtermcned to be 420,000 and 130,000 daltons respectively. The two purified enzymes were all fund to be homogencous by polyacrylamide gel electrophoresis.Denaturatioa and inactivation kinetics of AKPⅠ& AKPⅡby guanidine have been studied....

Two different forms of alkaline phosphatase ( AKPⅡ & AKP Ⅱ) were isolated and purified from Sinonovacula constricta by n-butanol extraction, ammonium sulfate fractionation, DEAE-cel-lulose chromatography and gel. filtration with Sephadex-G-200. Their molecular weights were drtermcned to be 420,000 and 130,000 daltons respectively. The two purified enzymes were all fund to be homogencous by polyacrylamide gel electrophoresis.Denaturatioa and inactivation kinetics of AKPⅠ& AKPⅡby guanidine have been studied. Rate constants of denaturation of AKPⅠ& AKPⅡin the presence of 0.5M, 2.0M and 4.0M gnanidine hydrochloride were measured by fluorescence method to be 3.0×10-3sec-1, 6.4×10-3sec-1, 10. 0×10-3sec-1 and 5.0×10-3 sec-1, 6.5×10-3sec-1, 9.2×10-3sec-1, respectively. In 0.5M guanidine, both AKPⅠ& AKPⅡ maintained 100% residual activity. In 2.0M guanidine, both the inactivation processes of AKPⅠand AKPⅡconsist of twofirst order reactions (fast and slow reoctions)with rate constants of 2.5×10-3 sec-1 and 0.68×10-3sec-1 for AKPⅠ, and 2.1×10-3sec-1 and 0.41×10-3sec-1for AKPⅡ. In 4.0M guanidine, AKPⅠ& AKPⅡlost their activities entirely. The results obtained are discussed.

缢蛏(Sinonovacula constricta)是一种海产重要经济贝类。本文报告从缢蛏中提取了分子量为420,000和130,000二种不同形式的碱性磷酸酶(简称APKⅠ和AKPⅡ),经不同浓度盐酸胍处理时酶的变性与失活的动力学过程。以荧光光谱为监测手段,测得AKPⅠ和AKPⅡ在0.5M、2.0、M、4.0M胍中的变性速度常数分别为0.0030秒~(-1)、0.0064秒~(-1)、0.0100秒~(-1)和0.0050秒~(-1)、0.0065秒~(-1)、0.0092秒~(-1);在0.5M胍中,AKPⅠ和AKPⅡ均保持100%的剩余活力;在2.0M胍中,AKPⅠ和AKPⅡ的失活均为快和慢二个一级的的过程,其失活速度常数分别为0.0025秒~(-1)(快)、0.00068秒~(-1)(慢)和0.0021秒~(-1)(快)、0.00041秒~(-1)(慢)。在4.0M胍中,AKPⅠ和AKPⅡ的活力完全丧失。本文中就这些结果进行了讨论。

Alkaline PhoPhatases (ALP) were extracted and purified from chicken skeleton, liver and intestine with butanol extraction and chromatography on DEAE-cellulose (DE-52) and concanavalin ASepharose 4B. Skeleton and liver tissues ALPs were separated with polyacrylamide gradient gel electrophoresis into two components, with molecular weights of 154000 and 353000(skeleton), 187000 and 353000(liver). Intestine ALP was electrophoretically separated into three components with molecular weights of 123000, 235000...

Alkaline PhoPhatases (ALP) were extracted and purified from chicken skeleton, liver and intestine with butanol extraction and chromatography on DEAE-cellulose (DE-52) and concanavalin ASepharose 4B. Skeleton and liver tissues ALPs were separated with polyacrylamide gradient gel electrophoresis into two components, with molecular weights of 154000 and 353000(skeleton), 187000 and 353000(liver). Intestine ALP was electrophoretically separated into three components with molecular weights of 123000, 235000 and 327000. The tissues ALPs were separated into two distinct peaks with DE-52 chromatography. The main peak was further purified with Con. A-Sepharose 4B affinity chromatography, then the properties were studied. The Km values of the skeleton, liver and intestine enzymes for the hydrolysis of disodium phenyl orthophosphate were 0.532 mmol/L, 0.452mmol/L and 0.472mmol/L,respectively. The tissue ALPs were different in thermal stability. The bone and liver ALPs by urea at 4mol/L decreased by 100% and 95%, respectively. At the same concentration the intestine enzyme decreased by 47%. Levamisole (2mmol/L) inhibited the enzymes from bone and liver by more than 50%, whereas the enzymes from intestine was inhibited by less than 20%. In contrast, L-phenylalanine inhibited intestine ALP to a much greater extent than the skeleton and liver ALPs.

用正丁醇抽提、DEAE纤维素(DE—52)和Con·A—Sepharase 4B柱层析等方法从鸡的骨、肝和肠组织中提取碱性磷酸酶(ALP)。提纯倍数分别为41倍(骨)、391倍(肝)和130倍(肠)。将粗提的ALP进行聚丙烯酰胺梯度凝胶电泳,骨、肝ALP均出现两条带,其分于量为154 000和353 000(骨),187 000和353 000(肝);肠ALP有3个组分,分子量为123 000、235 000和327 000。骨、肝、肠的ATP经DE—52柱层析均得到两个酶峰,其中总活性较大的峰(主峰)进行Con·A—Sepharose 4B柱层析后,其ALP对磷酸苯二钠的km值分别为0.532mM(骨)、0.452mM(肝)和0.472mM(肠)。骨、肝ALP对热敏感,而肠ALP则比较耐热,56℃作用9min,酶活性降低98.8%(骨)、95%(肝)和59.3%(肠)。尿素可抑制骨和肝ALP,肠ALP对尿素不大敏感。4 mol/L尿素对骨ALP活性抑制100%,对肝ALP 95%,对肠ALP仅为47%。2 mmol/L左旋咪唑能抑制骨ALP活性的57.1%、肝ALP的57.7%、肠ALP的15.7%。20 ...

用正丁醇抽提、DEAE纤维素(DE—52)和Con·A—Sepharase 4B柱层析等方法从鸡的骨、肝和肠组织中提取碱性磷酸酶(ALP)。提纯倍数分别为41倍(骨)、391倍(肝)和130倍(肠)。将粗提的ALP进行聚丙烯酰胺梯度凝胶电泳,骨、肝ALP均出现两条带,其分于量为154 000和353 000(骨),187 000和353 000(肝);肠ALP有3个组分,分子量为123 000、235 000和327 000。骨、肝、肠的ATP经DE—52柱层析均得到两个酶峰,其中总活性较大的峰(主峰)进行Con·A—Sepharose 4B柱层析后,其ALP对磷酸苯二钠的km值分别为0.532mM(骨)、0.452mM(肝)和0.472mM(肠)。骨、肝ALP对热敏感,而肠ALP则比较耐热,56℃作用9min,酶活性降低98.8%(骨)、95%(肝)和59.3%(肠)。尿素可抑制骨和肝ALP,肠ALP对尿素不大敏感。4 mol/L尿素对骨ALP活性抑制100%,对肝ALP 95%,对肠ALP仅为47%。2 mmol/L左旋咪唑能抑制骨ALP活性的57.1%、肝ALP的57.7%、肠ALP的15.7%。20 mmol/L L—苯丙氨酸能抑制肝ALP活性的12%、骨ALP的18.5%、肠ALP的57%。低浓度L—苯丙氨酸(1~15mmol/L)对骨ALP几乎没有抑制作用,而对肝ALP有一定抑制作用。上述结果表明,鸡的骨、肝和肠ALP可分为两型:一型为肠ALP,另一型为骨和肝ALP。

 
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