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acetate metabolism
相关语句
  乙酸代谢
     Cultivation and Foreign Gene Expression in Escherichia coli Mutant with Improved Acetate Metabolism
     大肠杆菌乙酸代谢突变株的培养和外源基因表达
短句来源
     It revealed that the integral VHb and acetate metabolism pathway (Pta Ack) block improved the growth of host strain under low dissolved oxygen conditions, enhanced the recombinant proteins production and reduced the accumulation of acetate harmful to bacterial growth.
     整合型vhb的表达及乙酸代谢途径 (Pta Ack)的缺陷 ,改善了宿主在贫氧条件下的生长 ,且促进了重组蛋白的表达。
短句来源
  乙酸盐代谢
     Studies on activities of PTA, AK, ICL and MS enzymes involved in the acetate metabolism of Corynebactenum glutamicum
     谷氨酸棒状杆菌乙酸盐代谢PTA、AK、ICL和MS酶活性研究
短句来源
     The four enzymes of acetate kinase (AK), phosphotransacetylase (PTA), isocitrate lyase (ICL) and malate systhase (MS) which are involved in the acetate metabolism of Corynebacterium glu-tamicum have been developed in their assay methods. The enzyme activities of AK, PTA, ICL and MS are analyzed comparatively while C- glutamicum grows on glucose or/and on acetate.
     从谷氨酸棒状杆菌乙酸盐代谢中涉及的磷酸转乙酰酶PTA、乙酸盐激酶AK、异柠檬酸裂解酶ICL和苹果酸合成酶MS 4种酶入手,建立了一套稳定的酶活性测定方法,并对这些酶在葡萄糖和乙酸盐不同碳源代谢中的酶活性特征进行了比较分析,发现碳代谢中存在葡萄糖效应;
短句来源
  “acetate metabolism”译为未确定词的双语例句
     A Study on the Anaerobic Acetate Metabolism in the Biological Phosphorus Removal Process
     生物除磷过程中乙酸盐厌氧代谢机理的研究
短句来源
     In this study, the anaerobic acetate metabolism was investigated in the biological phosphorus removal process.
     本文研究了生物除磷过程中乙酸盐厌氧代谢机理。
短句来源
  相似匹配句对
     Clinical application of ~(11)C-acetate PET metabolism imaging
     PET代谢显像剂~(11)C-乙酸盐在临床中的应用
短句来源
     METABOLISM OF FARREROL
     杜鹃素的体内代谢
短句来源
     Metabolism of curcumin
     姜黄素的代谢研究
短句来源
     Isolation identification and studies on metabolism of bacteria degrading polyvinyl acetate.
     聚乙酸乙烯酯降解菌的分离、鉴定及降解特性
短句来源
     Synthesis of Methenolone Acetate
     美替诺龙醋酸酯的合成
短句来源
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  acetate metabolism
Plasma total cholesterol and total triglyceride levels significantly correlated with changes in acetate metabolism (R2 = 0.7768 and R2 = 0.4787, respectively) and with changes in glucose metabolism (R2 = 0.6067 and R2 = 0.4506, respectively).
      
Acetate metabolism was not affected by NH4Cl, thus indicating that the tricarboxylic acid cycle was unchanged.
      
Comparison of linoleate, palmitate and acetate metabolism in rat ventral prostate
      
Phosphotransacetylase (Pta) [EC 2.3.1.8] plays a major role in acetate metabolism by catalyzing the reversible transfer of the acetyl group between coenzyme A (CoA) and orthophosphate: CH3COSCoA+HPO
      
Mevalonate was accumulated, indicating a redirection of acetate metabolism by the expressed enzyme.
      
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In this study, the anaerobic acetate metabolism was investigated in the biological phosphorus removal process. The experimental results demonstrate: under the anaerobic condition the poly-B-hydroxy butyrate content and the phosphorus release of the microorganism in the activated sludge increase with its acetate uptake linearly respectively.

本文研究了生物除磷过程中乙酸盐厌氧代谢机理。结果表明,在厌氧条件下,活性污泥的聚-β-羟基丁酸盐含量,无机磷酸盐释放量分别随乙酸盐吸收量的增加而呈线性关系上升。

A novel engineered strain G830 adoptable to high cell density fermentation by integrating bacterial hemoglobin vhb (Vitreoscilla hemoglobin gene) into thr operon in the chromosome of PA1 blocking Pta Ack metabolic pathway through the homologous recombination between the homologous fragments of integrated plasmid and that of chromosome. The engineered strain G830 was characterized by phenotype observation, PCR, thr mutant, acetate acid detection, Western blotting and VHb activity assays. In high...

A novel engineered strain G830 adoptable to high cell density fermentation by integrating bacterial hemoglobin vhb (Vitreoscilla hemoglobin gene) into thr operon in the chromosome of PA1 blocking Pta Ack metabolic pathway through the homologous recombination between the homologous fragments of integrated plasmid and that of chromosome. The engineered strain G830 was characterized by phenotype observation, PCR, thr mutant, acetate acid detection, Western blotting and VHb activity assays. In high dentity fermentation, the cellular respiration, energy metabolism, highest bacterial density and dry bacteria weight of the G830 strain were markedly better than control strains PA1 and BL21. The expression of recombinant prolyl endopeptidase (PEP) in G830 and PA1 under the above condition was high and stable. Their growth situation and fermentation parameters were similar with their parental strains without plasmids, and resided plasmids maintained stably in those strains. It revealed that the integral VHb and acetate metabolism pathway (Pta Ack) block improved the growth of host strain under low dissolved oxygen conditions, enhanced the recombinant proteins production and reduced the accumulation of acetate harmful to bacterial growth. In conclusion, the novel engineering strain G830 was adoptable to high cell density fermentation.

运用PCR方法 ,从磷酸乙酰转移酶 (Pta) 乙酸激酶 (Ack)代谢途径缺失菌株E .coliPA1染色体上 ,扩增出天冬氨酸激酶 I 高丝氨酸脱氢酶 I(thrA)和高丝氨酸激酶 (thrB)基因部分序列 ,构建了整合型重组质粒 pVHb Kan ;应用染色体 质粒同源重组的方法 ,将透明颤菌血红蛋白 (Vitreoscillahaemoglobin ,VHb)基因整合到大肠杆菌PA1染色体上的thr操纵子 ,构建了新型整合工程菌G830。在高密度发酵条件下 ,G830的细胞呼吸强度、能量代谢、最高菌密度和细胞干重 ,均明显优于对照菌株PA1和BL2 1;重组蛋白脯氨酰内肽酶在G830和PA1中获得稳定高表达 ;重组菌生长状况及发酵指标均与空宿主菌基本一致且表达质粒能维持较好的稳定性。整合型vhb的表达及乙酸代谢途径 (Pta Ack)的缺陷 ,改善了宿主在贫氧条件下的生长 ,且促进了重组蛋白的表达。该工程菌具有良好的氧耐受力 ,且乙酸积累得到大幅度降低 ,可作为适于高密度发酵的基因工程菌

To develop the blue white colony screening with promoter test vector in Corynebacterium glutamicum , two vectors pROH7 and pROHO were constructed and transformed into Corynebacterium glutamicum ATCC 14752. They carry a tetracyclin resistance determinant and the promoterless β galactosidase gene as a reporter gene behind two different promoter regions of the aceA gene of C. glutamicum , which encodes the enzyme isocitrate lyase, one of the key enzymes of acetate metabolism. Results were...

To develop the blue white colony screening with promoter test vector in Corynebacterium glutamicum , two vectors pROH7 and pROHO were constructed and transformed into Corynebacterium glutamicum ATCC 14752. They carry a tetracyclin resistance determinant and the promoterless β galactosidase gene as a reporter gene behind two different promoter regions of the aceA gene of C. glutamicum , which encodes the enzyme isocitrate lyase, one of the key enzymes of acetate metabolism. Results were shown that white colonies grew on the minimum medium containing glucose, whearas blue colonies grew on the minimum medium containing acetate; The colour intensity varied from pale blue in colonies harbouring the test vector pROH7 to dark blue in colonies harbouring the test vector pROHO. The colour of the colonies with the test vectors pROH7 or pROHO reflects the regulatory state of the cells. Two vectors pROH7 and pROHO were shown to be functional in the identification of C. glutamicum clones with altered regulation of the acetate metabolism.

在谷氨酸棒状杆菌乙酸盐代谢调控关键酶—异柠檬酸裂解酶 ace A基因两段启动子区域后面组装了无启动子的β-半乳糖苷酶报道基因 ,并且带上四环素抗性标记基因构建了两个检测质粒 p ROH7和 p ROHO.通过该两个质粒转化了谷氨酸棒状杆菌野生型菌株 Corynebacterium glutamicum ACTT14 75 2 ,分别获得了含有两个检测质粒的两种谷氨酸棒状杆菌 ,由此建立了谷氨酸棒状杆菌在乙酸盐和葡萄糖基质上的两个蓝白生长筛选系统 .结果显示当两种菌株生长在含有葡萄糖的基本培养基上时呈现白色菌落 ,而生长在含有乙酸盐的基本培养基上时呈现蓝色菌落 ;其中菌落颜色还随菌株中检测质粒p ROH7和 p ROHO的不同而呈现淡蓝色和深蓝色的变化 .含有检测质粒 p ROH7和 p ROHO菌落的颜色变化反映了细胞在葡萄糖和乙酸盐不同和碳源上的调控状态 ,该两个质粒在鉴定乙酸盐代谢中的调控变化方面是有积极意义

 
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