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copper-binding domains
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  铜离子结合区
     [Results] When 1-4 copper-binding domains of ATP7B gene were deleted one by one, the trafficking of ATP7B delayed or diminished.
     【结果】ATP7B基因1-4铜离子结合区逐个缺失后,ATP7B蛋白定向移动减慢至消失;
短句来源
     [Conclusion] The function of six copper-binding domains of ATP7B gene is different. The 1-4 domains induce trafficking of ATP7B within the cell, while the 5-6 copper-binding domains are directly involved in copper transport.
     【结论】ATP7B基因6个铜离子结合区功能不同,其中1-4结合区主要根据铜浓度诱导ATP7B蛋白细胞内定向移动,而5-6结合区则直接参与铜离子细胞外转运。
短句来源
     [Objective] The deletion mutant of copper-binding domains of ATP7B gene was constructed in order to investigate its function in hepatocytes of toxic milk mouse.
     【目的】构建ATP7B基因铜离子结合区缺失突变体,研究其在Tx小鼠肝细胞中的表达及功能。
短句来源
     When 5-6 copper-binding domains were deleted, copper transport stopped.
     5-6铜离子结合区缺失,细胞铜转运功能停滞。
短句来源
  “copper-binding domains”译为未确定词的双语例句
     The conserved region was 595 bp in length, which encoded 195 amino acids. The deduced amino acid sequence contained putative copper-binding domains characteristic of known PPOs, it had a significant homology to known PPO sequence from grape(Vitis vinifera ), tomato(Lycopersicon esculentum ), potato(Solanum tuberosum )and broad bean(Vicia faba ), were 71.3%, 80.8%, 79.8%, and 70.6%, respectively.
     PPO保守区之间的cDNA片段,测序表明该片段含595bp,编码195个氨基酸,推导的氨基酸序列包含了植物PPO的2个保守的铜结合区域,与葡萄(Vitisvinifera )、番茄(Lycopersiconesculentum)、马铃薯(Solanumtuberosum)和蚕豆(Viciafaba )PPO保守区的同源性分别为71.3%、80.8%、79.8%、70.6%。
短句来源
  相似匹配句对
     COPPER
     铜
短句来源
     They are DNA binding domains.
     它们的C末端分别含有SAP和SANTDNA结合结构域。
短句来源
     When 5-6 copper-binding domains were deleted, copper transport stopped.
     5-6铜离子结合区缺失,细胞铜转运功能停滞。
短句来源
     Study on Binding Eqilibrium of Gelatin with Copper
     明胶与Cu~(2+)的结合平衡研究
短句来源
     Progress in Research on DNA-Binding Protein Domains
     DNA结合蛋白结构域的研究进展
短句来源
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  copper-binding domains
The inferred yellow-poplar laccase gene products were highly related to one another (79-91% at the amino acid level) and showed significant similarity to other blue copper oxidases, especially with respect to the copper-binding domains.
      
A high degree of sequence conservation was found with the copper-binding domains of the 59 kDa tomato PPO as well as hemocyanins and tyrosinases from a wide diversity of taxa.
      
Alignments of the predicted amino acid sequences highlighted regions of variable sequence flanked by the highly conserved copper-binding domains that characterize members of this enzyme family.
      
The Atx1 copper chaperone binds Cu(I) and interacts directly with the copper-binding domains of a P-type ATPase copper transporter, its physiological partner.
      


Objective: To investigate the pathogenesis of Wilson disease (WD) by detecting its gene products encoded by WD gene (ATP7B). Methods Patients diagnosed as WD were analyzed by SDS-PAGE in conjunction with Western blot. Two antibodies were used, which are specially against the sixth copper binding domain (Anti-CuBD) and ATP binding domain (Anti-ABD) of WD protein. Results The WD proteins were not expressed in two patients when using antiCuBD, and poorly expressed when using anti-ABD. Conclusions WD...

Objective: To investigate the pathogenesis of Wilson disease (WD) by detecting its gene products encoded by WD gene (ATP7B). Methods Patients diagnosed as WD were analyzed by SDS-PAGE in conjunction with Western blot. Two antibodies were used, which are specially against the sixth copper binding domain (Anti-CuBD) and ATP binding domain (Anti-ABD) of WD protein. Results The WD proteins were not expressed in two patients when using antiCuBD, and poorly expressed when using anti-ABD. Conclusions WD is highly heterogeneous in clinical manifestations and inheritance pattern. Two WD patients might simultaneously have exon 5 mutations and exon 8 mutations. The study of WD gene products would probe into the pathogenesis of WD.

目的 对肝豆状核变性(WD)基因编码产物WD蛋白进行检测,探讨WD的发病机制。方法 应用Western-blot蛋白印迹技术对诊断为WD的患者进行WD蛋白的研究。结果 发现患者WD蛋白在肝内表达缺失或者含量改变。结论 WD患者可能同时存在有WD基因exons和exon5的突变,直接检测WD基因产物为进一步研究WD的病理机制奠定了基础。

The sweet potato (Ipomoea batatas L.) polyphenol oxidase (PPO) cDNA was cloned and sequenced. Firstly the conserved region of sweet potato PPO gene was cloned and sequenced through PCR using a pair of degenerate primers designed on the basis of analysis of cloned PPO gene. The conserved region was 595 bp in length, which encoded 195 amino acids. The deduced amino acid sequence contained putative copper-binding domains characteristic of known PPOs, it had a significant homology to known PPO sequence from...

The sweet potato (Ipomoea batatas L.) polyphenol oxidase (PPO) cDNA was cloned and sequenced. Firstly the conserved region of sweet potato PPO gene was cloned and sequenced through PCR using a pair of degenerate primers designed on the basis of analysis of cloned PPO gene. The conserved region was 595 bp in length, which encoded 195 amino acids. The deduced amino acid sequence contained putative copper-binding domains characteristic of known PPOs, it had a significant homology to known PPO sequence from grape(Vitis vinifera ), tomato(Lycopersicon esculentum ), potato(Solanum tuberosum )and broad bean(Vicia faba ), were 71.3%, 80.8%, 79.8%, and 70.6%, respectively. Then gene-specific primers designed on the basis of sequence of the conserved region of sweet potato PPO cDNA were used to amplify cDNA clones by 3′ rapid amplification of cDNA ends (3′ RACE) and 5′ rapid amplification of cDNA ends (5′ RACE). The results showed that the complete cDNA was 1 984 bp in length, including 16 bp of 5′ untranslated region,202 bp of 3′ untranslated region which contained a polyadenylation singal, AATAAA, and a 17 bp polyadenylation tail. The coding region encoded a peptide of 588 amino acid residues. The deduced amino acid sequence had a significant homology to the PPO sequence from grape, tomato, potato and broad bean, were 49%, 48%, 47% and 49%, respectively.

植物多酚氧化酶(polyphenoloxidase,PPO)是一类核编码的铜金属酶,含有2个保守的铜结合区域(CuA和CuB),N端和C端部分缺乏明显的同源性。根据植物PPO的结构特点,从2个保守的铜结合区域的氨基酸序列设计一对简并引物,扩增了甘薯(IpomoeabatatasL.)PPO保守区之间的cDNA片段,测序表明该片段含595bp,编码195个氨基酸,推导的氨基酸序列包含了植物PPO的2个保守的铜结合区域,与葡萄(Vitisvinifera )、番茄(Lycopersiconesculentum)、马铃薯(Solanumtuberosum)和蚕豆(Viciafaba )PPO保守区的同源性分别为71.3%、80.8%、79.8%、70.6%。在此基础上设计引物通过3'RACE和5'RACE的方法获得了甘薯PPOcDNA的3'端和5'端的片段。最后根据保守区的cDNA片段、3'RACE片段和5'RACE片段的序列信息进行拼接,推导出甘薯PPOcDNA的全部序列信息。结果表明:甘薯PPOcDNA全长含1984bp,有完整的阅读框,编码588个氨基酸。另外5'非翻译区16bp,3'非翻译区202bp,其中...

植物多酚氧化酶(polyphenoloxidase,PPO)是一类核编码的铜金属酶,含有2个保守的铜结合区域(CuA和CuB),N端和C端部分缺乏明显的同源性。根据植物PPO的结构特点,从2个保守的铜结合区域的氨基酸序列设计一对简并引物,扩增了甘薯(IpomoeabatatasL.)PPO保守区之间的cDNA片段,测序表明该片段含595bp,编码195个氨基酸,推导的氨基酸序列包含了植物PPO的2个保守的铜结合区域,与葡萄(Vitisvinifera )、番茄(Lycopersiconesculentum)、马铃薯(Solanumtuberosum)和蚕豆(Viciafaba )PPO保守区的同源性分别为71.3%、80.8%、79.8%、70.6%。在此基础上设计引物通过3'RACE和5'RACE的方法获得了甘薯PPOcDNA的3'端和5'端的片段。最后根据保守区的cDNA片段、3'RACE片段和5'RACE片段的序列信息进行拼接,推导出甘薯PPOcDNA的全部序列信息。结果表明:甘薯PPOcDNA全长含1984bp,有完整的阅读框,编码588个氨基酸。另外5'非翻译区16bp,3'非翻译区202bp,其中包括1个多聚腺苷酸化信号AATAAA,以及1个含17个腺苷酸的ploy(A)尾。由甘薯PPOcDNA推导的氨基酸序列与由葡萄、番茄、马铃薯和蚕豆PPOcDNA推导的氨基酸序列进行比较,它们之间存在较明显的同源性,同源性分别为49%、48%、47%和49%。

[Objective] The deletion mutant of copper-binding domains of ATP7B gene was constructed in order to investigate its function in hepatocytes of toxic milk mouse. [ Methods] The deletion mutant expression vectors of ATP7B gene were constructed by means of long distance PCR and plas-mid-recombination. The hepatocytes of toxic milk mouse were transfected by recombinant plasmid mediated by liposome, cultured in high and low copper concentration. The trafficking of mutant ATP7B and its ability to transport copper...

[Objective] The deletion mutant of copper-binding domains of ATP7B gene was constructed in order to investigate its function in hepatocytes of toxic milk mouse. [ Methods] The deletion mutant expression vectors of ATP7B gene were constructed by means of long distance PCR and plas-mid-recombination. The hepatocytes of toxic milk mouse were transfected by recombinant plasmid mediated by liposome, cultured in high and low copper concentration. The trafficking of mutant ATP7B and its ability to transport copper were observed. [Results] When 1-4 copper-binding domains of ATP7B gene were deleted one by one, the trafficking of ATP7B delayed or diminished. When 5-6 copper-binding domains were deleted, copper transport stopped. [Conclusion] The function of six copper-binding domains of ATP7B gene is different. The 1-4 domains induce trafficking of ATP7B within the cell, while the 5-6 copper-binding domains are directly involved in copper transport.

【目的】构建ATP7B基因铜离子结合区缺失突变体,研究其在Tx小鼠肝细胞中的表达及功能。【方法】利用长片断PCR和质粒重组技术构建不同铜离子结合区的缺失体,脂质体介导转染Tx小鼠肝脏细胞,然后进行高铜、低铜孵育,观察ATP7B突变蛋白在细胞内的定向移动及对铜离子转运功能的影响。【结果】ATP7B基因1-4铜离子结合区逐个缺失后,ATP7B蛋白定向移动减慢至消失;5-6铜离子结合区缺失,细胞铜转运功能停滞。【结论】ATP7B基因6个铜离子结合区功能不同,其中1-4结合区主要根据铜浓度诱导ATP7B蛋白细胞内定向移动,而5-6结合区则直接参与铜离子细胞外转运。

 
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