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telomeric restriction fragment
相关语句
  端粒限制性片段长度
     TELOMERASE ACTIVITY AND TELOMERIC RESTRICTION FRAGMENT IN GASTRIC TUMOR AND PREMALIGNANT LESIONS:DETERMINATION AND ITS SIGNIFICANCE
     胃癌及癌前组织中端粒酶活性及端粒限制性片段长度的检测及其临床意义
短句来源
  端粒状态
     Analysis of telomeric restriction fragment in gastric cancer and its premalignant lesions
     胃癌及癌前组织端粒状态的分析
短句来源
  “telomeric restriction fragment”译为未确定词的双语例句
     Analysis on genetic instability and telomeric restriction fragment length of gastric cancer in vitro
     胃癌粘膜基因不稳与染色体端粒长度改变的研究
短句来源
     EFFECTS OF EXOGENOUS hTERT TRANSFECTION ON TELOMERIC RESTRICTION FRAGMENT,TELOMERASE ACTIVITY AND ITS SUBUNITS EXPRESSION OF HUMAN EMBROYNIC FIBROBLASTS
     hTERT基因转染对人胚胎成纤维细胞端粒长度、端粒酶活性及其亚单位的影响
短句来源
     Changes of telomeric restriction fragment in colorectal carcinoma.
     大肠癌端粒长度变化的研究
短句来源
     Telomerase activity (TA) was detected with telomeric repeat amplification protocol(TRAP) assay and the mean length telomeric restriction fragment (TRF) with Southern blot.
     采用TRAP法检测了176例不同病变胃粘膜组织端粒酶活性(TA),同时采用Southern杂交技术检测了35例胃癌及其相应的19例癌旁和13例手术切缘组织端粒限制性片段(TRF)长度。
短句来源
     In order to explore the effects of exogenous human telomerase reverse transcriptase (hTERT/hTRT/hEST2) on telomeric restriction fragment (TRF), telomerase activity and its subunits expression in human embryonic fibroblasts (hEFs), hTERT sense eukaryotic expression vector pIRES2 EGFP hTERT was constructed with DNA recombinant technique and then transfected into primary hEFs by Lipofectin method.
     为了探讨外源性人端粒酶蛋白催化亚单位 (hTERT)基因转染对人胚胎成纤维细胞 (hEFs)端粒长度、端粒酶活性及其亚单位的影响 ,采用基因重组技术构建hTERT全长cDNA正义荧光真核表达载体 ,并采用脂质体法将正义重组质粒pIRES2 EGFP hTERT及空载质粒pIRES2 EGFP分别转染原代培养hEFs,检测转染细胞端粒长度、端粒酶活性及端粒酶亚单位的变化。
短句来源
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  相似匹配句对
     Changes of telomeric restriction fragment in colorectal carcinoma.
     大肠癌端粒长度变化的研究
短句来源
     Analysis of telomeric restriction fragment in gastric cancer and its premalignant lesions
     胃癌及癌前组织端粒状态的分析
短句来源
     Analysis on genetic instability and telomeric restriction fragment length of gastric cancer in vitro
     胃癌粘膜基因不稳与染色体端粒长度改变的研究
短句来源
     Objective To explore the role of telomeric restriction fragment(TRF) in the progress of colorectal tumorigenesis.
     目的检测大肠癌、癌旁组织和正常大肠粘膜组织端粒长度变化,探讨其在大肠癌发生发展中的临床意义。
短句来源
     TELOMERASE ACTIVITY AND TELOMERIC RESTRICTION FRAGMENT IN GASTRIC TUMOR AND PREMALIGNANT LESIONS:DETERMINATION AND ITS SIGNIFICANCE
     胃癌及癌前组织中端粒酶活性及端粒限制性片段长度的检测及其临床意义
短句来源
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  telomeric restriction fragment
Telomeric restriction fragment (TRF) lengths, as an indicator of telomere length, were determined for different tissues by Southern hybridization analysis.
      
Comparative analysis of telomeric restriction fragment lengths in different tissues of Ginkgo biloba trees of different age
      
By comparing telomeric restriction fragment (TRF) lengths at different developmental stages from embryos to seedlings, a fluctuant tendency towards variation was found in these samples.
      
Dynamic changes of telomeric restriction fragment (TRF) lengths in cells during the developmental process from embryos to seedli
      
Moreover, a new telomeric restriction fragment is present in some hybrids.
      


A recent study demonstrated that telomere reduction could be observed during multi stage astrocarcinogenesis,but the length of telomeric restriction fragments (TRFs)was maintained relatively stable in primary and recurrent glioblastomas.To elucidate possible existence of telomere repair mechanism(s) in those tumor cells,telomerase activities in the protein extracts of seven human and one rat glioblastoma cell lines as well as in normal adult rat brain were analyzed with telomeric repeat amplification...

A recent study demonstrated that telomere reduction could be observed during multi stage astrocarcinogenesis,but the length of telomeric restriction fragments (TRFs)was maintained relatively stable in primary and recurrent glioblastomas.To elucidate possible existence of telomere repair mechanism(s) in those tumor cells,telomerase activities in the protein extracts of seven human and one rat glioblastoma cell lines as well as in normal adult rat brain were analyzed with telomeric repeat amplification protocol (TRAP)with or without RNase (DNase free)treatment.It was revealed that after being electrophoresized in polyacrylamide gel,telomeric PCR fragments could be detected in all eight glioma samples but neither in their normal counterpart nor in the same eight samples pre treated with DNase free RNase,which confirmed the specificity of the TRAP results and suggested that the PCR amplification in TRAP reaction solutions were mediated,at least largely,by telomerase.In conclusion,the data show a generality of telomerase activities in glioblastomas and implicate a potential biological and/or pharmaceutical approach for the treatment of glioblastomas by downregulation of telomerase activities.

端粒缩短见于星形细胞瘤发展过程中,但其长度在胶质母细胞瘤/细胞系相对稳定,提示胶质瘤细胞内存在端粒修复机制的可能性.为证实此点,利用端粒重复片段扩增技术(TRAP),对8株人/大鼠多形胶质母细胞系的蛋白提取液中端粒酶活性加以测定.结果显示:8例胶质瘤样本的反应液均可见端粒PCR扩增片段;用无DNase的RNase事先处理蛋白提取液,可明显降低或消除PCR产物的出现,说明TRAP反应中的PCR扩增是在端粒酶的介导下进行而非DNA污染或其它端粒修复因子所致.从而不但建立起检测人癌细胞内端粒酶活性的可靠方法,也为针对端粒酶的胶质母细胞瘤生物/药物治疗提供了实验依据.

The lengths of telomeric restriction fragments(TRF) in 16 cases with early gastric cancer, 52 with advanced gastric cancer, 26 with intestinal metaplasia, 67 with atypical hyperplasia and adjacent normal tissues were analysed by hybridization of nucleic acids directly in agarose gels. Significant reductions in mean telomeric lengths were observed in all gastric carcinomas and precancerosis excluding mild atypical hyperplasia tissues when compared to normal gastric tissues.15 TRF reduced and 6 TRF...

The lengths of telomeric restriction fragments(TRF) in 16 cases with early gastric cancer, 52 with advanced gastric cancer, 26 with intestinal metaplasia, 67 with atypical hyperplasia and adjacent normal tissues were analysed by hybridization of nucleic acids directly in agarose gels. Significant reductions in mean telomeric lengths were observed in all gastric carcinomas and precancerosis excluding mild atypical hyperplasia tissues when compared to normal gastric tissues.15 TRF reduced and 6 TRF elongated in all cancer tissues, and 22 TRF deleted and 12 TRF prolonged in all precancerosis tissues. Alterations of TRF length were in line with cancer stage and precancerosis grade. The results indicated that telomeric abnormal status may be one of important biologic markers of gastric precancerosis toward malignant transformation and cancerous progression.

为观察胃癌及癌前病变组织中的端粒状态及其与该类肿瘤发生发展的关系,以琼脂糖DNA直接杂交技术检测16例早期胃癌、52例进展期胃癌、26例胃粘膜肠化及67例异型增生组织中端粒限制性片段(TRF)的长度。发现胃癌及癌前病变组织中的平均TRF长度均比相应正常胃组织缩短。其中胃癌有15例TRF缩短,6例TRF延长,癌前病变中有22例TRF缩短,12例TRF延长。且TRF的这种变化与胃癌的阶段及癌前病变的程度相一致。结果表明,端粒长度改变可能是反映胃粘膜细胞癌变及胃癌进展的一个重要生物学标志

Telomerase activity (TA) was detected with telomeric repeat amplification protocol(TRAP) assay and the mean length telomeric restriction fragment (TRF) with Southern blot. The results showed that TA vlues in chronic atrophy gastritis (CAG), intestinal metaplasia(IM), dysplasia (Dys) and gastric cancer (GC) were 24 6%(14/17), 38 9%(7/18), 37 5%(3/8) and 92 3%(60/65) respectively, those were higher than that in normal tissues (N) ( P <0 05 0 01). TA was significantly higher in group of GC than...

Telomerase activity (TA) was detected with telomeric repeat amplification protocol(TRAP) assay and the mean length telomeric restriction fragment (TRF) with Southern blot. The results showed that TA vlues in chronic atrophy gastritis (CAG), intestinal metaplasia(IM), dysplasia (Dys) and gastric cancer (GC) were 24 6%(14/17), 38 9%(7/18), 37 5%(3/8) and 92 3%(60/65) respectively, those were higher than that in normal tissues (N) ( P <0 05 0 01). TA was significantly higher in group of GC than in groups of CAG, IM and Dys ( P <0 01). Frequency and levels of TA were not associated with any clinicopathological parameters. The mean TRF length in tumor smaller, same or longer than that in the corresponding adjacent tissues was 57 1%(20/35),34 3%(12/35) or 7 6%(3/35) respectively. The mean TRF lengths from groups N, CAG, IM, Dys to GC decreased in order, and significantly longer in the groups of N, CAG than in the group of G ( P <0 01). The mean TRF length was not related with the patients' gender, tumor size, differentiation, clinical stage and the levels of TA, but with patients' age. TA detected not only in GC tissues also in some precancerous lesions and diseases indicate that it maybe play a crucial role in the development of GC. The mean TRF length might not be an accurate biomarker in cellular immortality.

采用TRAP法检测了176例不同病变胃粘膜组织端粒酶活性(TA),同时采用Southern杂交技术检测了35例胃癌及其相应的19例癌旁和13例手术切缘组织端粒限制性片段(TRF)长度。结果显示:①176例不同胃粘膜病变中,慢性萎缩性胃炎(CAG)、肠上皮化生(IM)、异型增生(Dys)及胃癌(GC)TA阳性检出率分别为24.6%、38.5%、37.5%及92.3%,而正常组织(N)未检出TA,明显低于以上各组(P<0.01~0.05),癌组织TA阳性率亦明显高于CAG、IM及Dys组(P<0.01),TA阳性检出率与患者临床病理指标无明显相关性;②TRF长度随N→CAG→IM→Dys→GC有逐渐缩短的趋势,但仅N及CAG组较GC组明显延长(P<0.01),与癌旁和(或)手术切缘组织比较,肿瘤组织TRF缩短者占20(57.1%),无变化和延长者占15(42.9%),二者比较差异无显著性意义(P>0.05),TRF长度与性别、肿瘤大小、分化程度、临床分期及TA无明显相关性,但与年龄有一定的关系。TA不仅在胃癌组织中可以检测到,而且在胃粘膜癌前病变或疾病中亦有表达,提示TA的检测在胃粘膜癌变的早期诊断和预测方面具?

 
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