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clone and growth
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  “clone and growth”译为未确定词的双语例句
     ③ Mononuclear cells were cultured in high glucose DMEM and low glucose DMEM (containing 0.1 volume fraction fetal calf serum), and others were the same, the number of cell clone was counted 72 hours later. The number of cell clone and growth state were compared in different media.
     ③分别用高糖和低糖DMEM培养液(含体积分数0.1的胎牛血清)培养单个核细胞,其他条件相同,从72h后对所形成的细胞克隆进行计数,对两种培养基形成的细胞克隆和生长状况进行比较。
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This paper sums up the results obtained from more-than-ten-year studies of the fertilizer practice through nutrient diagnosis(leaf analysis)of rubber trees. It falls into three parts: the time for sampling, the critical values for nutrient diagnosis and the effect of trial application on plantations,Investigations on the variation of leaf nutrient contents in different Stowing stages of rubber trees in South China showed that less fluctuation occurred in the contents of nitrogen, phosphorus, potassium, calcium...

This paper sums up the results obtained from more-than-ten-year studies of the fertilizer practice through nutrient diagnosis(leaf analysis)of rubber trees. It falls into three parts: the time for sampling, the critical values for nutrient diagnosis and the effect of trial application on plantations,Investigations on the variation of leaf nutrient contents in different Stowing stages of rubber trees in South China showed that less fluctuation occurred in the contents of nitrogen, phosphorus, potassium, calcium and magnesium in the lower shaded leaves during the period from July to September, which is therefore dasirable for sampling to make leaf analysis and determine the fertilizer requirements, because the nutrient analysis of leaves collected in this period is more accurate.Studies on leaf nutrient eontents of and fertilizer effect on the rubber trees differing in clone and growth on different soil types under different managements brought about the following critical values for healthy, normal-yielding rubber trees: N 3.2-3.4%, P 0.21-0.23%, K 0.9-1.1%, Ca 0.8-1.0%, Mg 0.35-0.45%. This method tends to result in accurate diagnosis of the nutrient status of rubber trees. Fertilization based on the diagnosis can enhance the effect of fertilizers, thereby increasing the yield by 10-20%. The results of field experiments proved that this practice is effective and suitable for rubber enltivation in South China.

本文总结了十多年来橡胶树营养诊断(叶片分析)指导施肥技术的研究结果,包括叶片采样时间、营养诊断指标和生产上试用的效果。结合我国橡胶树生长情况,研究不同时期叶片养分含量的差异后,确认低部位荫蔽叶片的氮、磷、钾、钙、镁养分含量的差异在7—9月间较小,与施肥的关系表现显著。故以7—9月为叶片分析诊断橡胶树需肥量的适宜采样时间。在此期间采样,诊断的准确性高。研究了不同土壤类型上不同品种、不同生长类型、不同管理下的橡胶树叶片养分含量和施肥效应后,得出生长健康、产量正常的橡胶树叶片营养指标是:氮3.2~3.4%,磷0.21~0.23%,钾0.9~1.1%,钙0.6~1.0%,镁0.35~0.45%。应用此项技术,可以确切地诊断胶树营养状况,据此对症施肥,可以提高肥效,增加产量10~20%。经过田间试验应用的结果,这是符合我国情况的有效技术。

The distributions of female cone, male cone and seed cone in the crown of Chinese fir ( Cunningbania laceolata Lamb. Hook.) were investigated in seed orchard by growth age section. The results show that significant differences about these characteristic values existed among clones and growth age sections. On the other hand, the setting ratio varies greatly with clones, but not with growth age sections. The clones of low output accounted for over 50% of all clones in seed...

The distributions of female cone, male cone and seed cone in the crown of Chinese fir ( Cunningbania laceolata Lamb. Hook.) were investigated in seed orchard by growth age section. The results show that significant differences about these characteristic values existed among clones and growth age sections. On the other hand, the setting ratio varies greatly with clones, but not with growth age sections. The clones of low output accounted for over 50% of all clones in seed orchard and the distributions of female cone, male cone and seed cone in the crown display regular changes, but are greatly different from that in normal forest land.

根据杉木树冠各部位的年龄大小,将杉木树冠划分为若干年龄段。通过对各年龄段♀、♂球花(果)数的调查分析,结果表明:杉木种子园中各无性系♀、♂球花(果)数以及座果率的差异都极其显著,而且低产无性系占一半以上;不同年龄段上的♀、♂球花(果)数的差异也达到极其显著水平并呈规律性变化,但各年龄段的座果率基本一致。

AIM: To observe the influence of differential conditions on human umbilical cord blood derived mesenchymalstem cells. METHODS: The experiment was conducted at the Department of Histology and Embryology, Capital Medical University from October 2004 to October 2005. ①Human umbilical cord blood was anticoagulated by sodium citrate and heparin sodium. Mononuclear cells isolated from human umbilical cord blood by density gradient centrifugation were counted and stained with trypan blue. Number of the mononuclear...

AIM: To observe the influence of differential conditions on human umbilical cord blood derived mesenchymalstem cells. METHODS: The experiment was conducted at the Department of Histology and Embryology, Capital Medical University from October 2004 to October 2005. ①Human umbilical cord blood was anticoagulated by sodium citrate and heparin sodium. Mononuclear cells isolated from human umbilical cord blood by density gradient centrifugation were counted and stained with trypan blue. Number of the mononuclear cells was compared. ② Mononuclear cells were cultured with three different density 1×109 L-1, 2×109 L-1 and 5×109 L-1. Morphology characteristics of human umbilical cord blood derived mesenchymal stem cells were observed by inverted microscope. Flow cytometry was used to detect immune phenotype of this kind of cell. ③ Mononuclear cells were cultured in high glucose DMEM and low glucose DMEM (containing 0.1 volume fraction fetal calf serum), and others were the same, the number of cell clone was counted 72 hours later. The number of cell clone and growth state were compared in different media. RESULTS: ① Number of mononuclear cells of human umbilical cord blood that anticoagulated by different anticoagulants and the cell growth: Number of Mononuclear cells that anticoagulated by heparin sodium was more and the cell growth was significantly better than those anticoagulated by sodium citrate (P < 0.05). ②Adherence growth state of human umbilical cord blood derived mesenchymalstem cells in different density and the immunophenotype: A mass of adhered cells were observed in 5×109 L-1 cultured but not in low cells density culture. Seventy-two hours later mononuclear cells began to adhere to the wall and form cell clone. Ten days later fusiform cells appeared, and during culture cells formed fusiform gradually. This type of cells were confirmed that it is non-hematopoietic mesenchymal stem cells by flow cytometry. ③Number of cell clone formed in two culture medium and the growth state: Number of cell clone in low glucose DMEM had significant difference as compared with that in high glucose DMEM (P < 0.05) 10 days later. CONCLUSION: ①The heparin sodium, as preserving fluid of human umbilical cord blood, can obtain a mass of mononuclear cells, and it is helpful for the culture of human umbilical cord blood derived mesenchymal stem cells. ②Adherence of human umbilical cord blood derived mesenchymal stem cells are enhanced in the density of 5×109 L-1. ③Low glucose DMEM, as a medium of human umbilical cord blood derived mesenchymal stem cells, is helpful to form and maintain cell clone, keep the characteristic of stem cell for long time.

目的:观察不同条件对人脐血间充质干细胞培养的影响。方法:实验于2004-10/2005-10在首都医科大学组织学胚胎学教研室实验室完成。①用复方枸橼酸血液保存液和肝素钠两种不同的抗凝血剂保存的人脐带血,经密度梯度离心法分离新鲜脐带血,获取的单个核细胞,经椎虫蓝染色进行活细胞计数,比较所获得的单个核细胞数。②用不同密度1×109L-1,2×109L-1,5×109L-1接种单个核细胞,倒置显微镜下观察细胞形态。用流式细胞仪检测所培养的细胞免疫表型。③分别用高糖和低糖DMEM培养液(含体积分数0.1的胎牛血清)培养单个核细胞,其他条件相同,从72h后对所形成的细胞克隆进行计数,对两种培养基形成的细胞克隆和生长状况进行比较。结果:①不同抗凝血剂抗凝的人脐带血所获得的单个核细胞数及细胞生长状态:用肝素钠抗凝血剂保存的人脐带血所获得的单个核细胞数和细胞生长状态明显优于用复方枸橼酸血液保存液保存的人脐带血(P<0.05)。②不同接种密度人脐血间充质干细胞的贴壁生长状态及人脐血间充质干细胞的免疫表型:较低密度接种细胞并不能形成大量贴壁细胞,而以5×109L-1密度接种,72h后单个核细胞开始贴壁并开始形成细胞克隆,在10d...

目的:观察不同条件对人脐血间充质干细胞培养的影响。方法:实验于2004-10/2005-10在首都医科大学组织学胚胎学教研室实验室完成。①用复方枸橼酸血液保存液和肝素钠两种不同的抗凝血剂保存的人脐带血,经密度梯度离心法分离新鲜脐带血,获取的单个核细胞,经椎虫蓝染色进行活细胞计数,比较所获得的单个核细胞数。②用不同密度1×109L-1,2×109L-1,5×109L-1接种单个核细胞,倒置显微镜下观察细胞形态。用流式细胞仪检测所培养的细胞免疫表型。③分别用高糖和低糖DMEM培养液(含体积分数0.1的胎牛血清)培养单个核细胞,其他条件相同,从72h后对所形成的细胞克隆进行计数,对两种培养基形成的细胞克隆和生长状况进行比较。结果:①不同抗凝血剂抗凝的人脐带血所获得的单个核细胞数及细胞生长状态:用肝素钠抗凝血剂保存的人脐带血所获得的单个核细胞数和细胞生长状态明显优于用复方枸橼酸血液保存液保存的人脐带血(P<0.05)。②不同接种密度人脐血间充质干细胞的贴壁生长状态及人脐血间充质干细胞的免疫表型:较低密度接种细胞并不能形成大量贴壁细胞,而以5×109L-1密度接种,72h后单个核细胞开始贴壁并开始形成细胞克隆,在10d后出现梭形细胞,培养过程中细胞形态逐渐形成均一梭形。用流式细胞仪检测所培养的细胞为非造血间充质干细胞。③两种培养基形成的细胞克隆数和生长状况:在10d以后,用低糖DMEM组培养的单个核细胞形成的细胞克隆数与高糖DMEM组相比较差异有显著性意义(P<0.05)。结论:①肝素钠作为人脐带血保存液能获得大量的单个核细胞,有利于人脐血间充质干细胞的培养。②5×109L-1细胞接种密度有利于人脐血间充质干细胞贴壁生长。③用低糖DMEM作为培养人脐血间充质干细胞的培养基有利于细胞克隆的形成和维持,对保持人脐血间充质干细胞特性有良好作用。

 
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