助手标题  
全文文献 工具书 数字 学术定义 翻译助手 学术趋势 更多
查询帮助
意见反馈
   human fetal tissue 的翻译结果: 查询用时:0.01秒
图标索引 在分类学科中查询
所有学科
基础医学
更多类别查询

图标索引 历史查询
 

human fetal tissue
相关语句
  “human fetal tissue”译为未确定词的双语例句
     Distribution of Human New Gene-era in Human Fetal Tissue:An Immunohistochemical Study
     人类新基因era基因组织分布的免疫组织化学研究
短句来源
     the expression levels of M-CSF and M-CSF-R in HCC tissues were significantly higher than that of human fetal tissue (P<0.05, P<0.05) and normal liver tissue (P<0.01, P<0.01).
     肝癌患者外周血单个核细胞中M-CSF/M-CSF-R表达水平较高,提示肝癌患者M-CSF的高表达与炎症反应有关。
短句来源
     Objective To explore a new oval cell marker by examining the expression of nestin in human fetal hepatic stem cells in various stages during human fetal development. Methods Human fetal tissue samples of various developmental stages were obtained and fixed for preparation of paraffin sections.
     目的采用免疫组织化学方法标记不同发育时期人胚胎肝干细胞中巢蛋白(Nestin)的表达,探讨肝干细胞新的表面标志蛋白。
短句来源
  相似匹配句对
     EPITHELIUM OF HUMAN FETAL ENDOMETRIUM
     人胎儿子宫内膜的上皮细胞
短句来源
     human;
     human ;
短句来源
     ULTRASTRUCTURE OF HUMAN FETAL EPIDIDYMIDES
     对胎儿附睾超微结构的加龄研究
短句来源
     INVESTIGATION ON HUMAN FETAL PANCREAS TISSUE CULTURE
     人胎胰腺组织细胞培养的研究
短句来源
     Expression of human annexin V in different fetal tissues
     人膜联蛋白在胎儿不同组织中的表达
短句来源
查询“human fetal tissue”译词为用户自定义的双语例句

    我想查看译文中含有:的双语例句
例句
为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法,我们为您准备了出自英文原文的大量英语例句,供您参考。
  human fetal tissue
Role of human fetal tissue transplantations in rheumatoid arthritis
      
Changes in the hormonal profile after human fetal tissue transplantation are studied in ovariectomized women of reproductive age.
      
Autonomic reactions in patients undergoing total oophorectomy and their changes after human fetal tissue transplant
      
The possibility of using the delayed-type hypersensitivity reaction to extract of human fetal tissue for the diagnosis and monitoring of treatment efficacy in cancer patients is demonstrated.
      
Breast examinations carried out 6-8 months after transplantation of human fetal tissue showed no deterioration of any kind.
      
更多          


Objective: To observe the distribution of human new gene-era in human fetal different tissues by using expressed protein of h-era and h-era C-terminal domain in E.coli and prepared polycloning antiserum against human era. Method: Gene clone, PCR, protein expressing, purifying, Western-blot identify and immunohistochemistry staining were used. Human era (h-era) cDNA, h-era and C-terminal domain gene were amplified by PCR using a plasmid containing h-era c-DNA as a template, E. colt TAP106 transformed with the...

Objective: To observe the distribution of human new gene-era in human fetal different tissues by using expressed protein of h-era and h-era C-terminal domain in E.coli and prepared polycloning antiserum against human era. Method: Gene clone, PCR, protein expressing, purifying, Western-blot identify and immunohistochemistry staining were used. Human era (h-era) cDNA, h-era and C-terminal domain gene were amplified by PCR using a plasmid containing h-era c-DNA as a template, E. colt TAP106 transformed with the recombinant plasmid pDH-h-era was introduced at 42 C to express h-era C-terminal domain protein. Antiserum against h-era was prepared by immunizing rabbit with h-era C-terminal domain protein purified by SDS-PAGE. E. colt BL21 (DE3) transformed with the recombinant plasmid pRSET-C-h-era was induced with IPTG to express (His) 6-h-era fusion protein that was applied for Western-blot analysis to identify the antiserum. Finally using the characteristic antibody to Immunohistochemical stain human fetal tissue. Results: The expressed h-era C-terminal domain and (his) 6-h-era fusion protein were about 40% and 80% of total bacteria protein respectively. The new hera protein had different expressing level by using the purifying characteristic polycloning antibody in fetal different tissues in our study. Conclusion f The h-era C-terminal domain protein and (His) 6-h-era fusion protein were highly expressed in E.coli. and the rabbit antiserum against h-era was successfully prepared and characterized. The h-era protein might be a new functional protein and it could express in most normal human fetal tissues.

目的:为了研究人era基因这一人类新基因表达产物的组织分布情况.方法:根据人era序列设计并合成扩增引物,用PCR法从pUC19-hera质粒中扩增人era基因(h-era)全长cDNA和h-ERAC端的结构域基因,并分别克隆到(His)6融合表达载体pRSET-C和非融合表达载体pDH中,诱导表达(HIS)6-h-ERA 融合蛋白和 h-ERAC端结构域蛋白.用 h-ERAC端结构域蛋白免疫新西兰白兔,制备兔抗 h-ERA抗血清.用 Western-blot对兔抗h-ERA 抗血清.进行了鉴定,并成功地制备了兔抗h-ERA血清.利用在大肠杆菌中高表达、纯化的(His)6-h-ERA融合蛋白,对已制备的兔抗人 ERA抗血清进行进一步鉴定纯化获得抗 h-ERA的特异性多抗;再对 4月~5月人胎儿心、肝、肺、脾、肾等脏器和胃、小肠、睾丸、胸腺、胰脏及胆囊进行免疫组织化学染色.结果:在人类4月~5个月胎儿心、肝、肺、脾、肾脏等脏器和胃、小肠、睾丸中均有免疫反应阳性产物出现.胰脏、心、肺脏等是高表达,脾脏、胃、小肠是中等表达,睾丸、肝脏、肾脏呈低表达,胆囊、胸腺无免疫阳性表达产物出现.结论:人 ERA在人类正常胎儿不...

目的:为了研究人era基因这一人类新基因表达产物的组织分布情况.方法:根据人era序列设计并合成扩增引物,用PCR法从pUC19-hera质粒中扩增人era基因(h-era)全长cDNA和h-ERAC端的结构域基因,并分别克隆到(His)6融合表达载体pRSET-C和非融合表达载体pDH中,诱导表达(HIS)6-h-ERA 融合蛋白和 h-ERAC端结构域蛋白.用 h-ERAC端结构域蛋白免疫新西兰白兔,制备兔抗 h-ERA抗血清.用 Western-blot对兔抗h-ERA 抗血清.进行了鉴定,并成功地制备了兔抗h-ERA血清.利用在大肠杆菌中高表达、纯化的(His)6-h-ERA融合蛋白,对已制备的兔抗人 ERA抗血清进行进一步鉴定纯化获得抗 h-ERA的特异性多抗;再对 4月~5月人胎儿心、肝、肺、脾、肾等脏器和胃、小肠、睾丸、胸腺、胰脏及胆囊进行免疫组织化学染色.结果:在人类4月~5个月胎儿心、肝、肺、脾、肾脏等脏器和胃、小肠、睾丸中均有免疫反应阳性产物出现.胰脏、心、肺脏等是高表达,脾脏、胃、小肠是中等表达,睾丸、肝脏、肾脏呈低表达,胆囊、胸腺无免疫阳性表达产物出现.结论:人 ERA在人类正常胎儿不同组织中?

Objective To explore a new oval cell marker by examining the expression of nestin in human fetal hepatic stem cells in various stages during human fetal development. Methods Human fetal tissue samples of various developmental stages were obtained and fixed for preparation of paraffin sections. The expression of nestin, generally recognized as a specific marker for neural precursor cells, in fetal hepatic stem cells was detected using immunocytochemical staining (ABC method)....

Objective To explore a new oval cell marker by examining the expression of nestin in human fetal hepatic stem cells in various stages during human fetal development. Methods Human fetal tissue samples of various developmental stages were obtained and fixed for preparation of paraffin sections. The expression of nestin, generally recognized as a specific marker for neural precursor cells, in fetal hepatic stem cells was detected using immunocytochemical staining (ABC method). Results Nestin-positive oval cells, characterized to be hepatic stem cells, were found in the limiting plate, which arranged tightly in monolayer to form ductules that encapsulated the early-stage portal area in a sheath-like fashion, and also partially encapsulated the primary portal area. With the development of the secondary portal areas, the nestin-positive oval cells gradually centered around the Hering canal. In addition, some nestin-positive mononuclear cells were found scattering in the hepatic cords and sinusoids, especially in the mesenchymal tissue of the portal areas. Conclusion Nestin-positive oval cells exist in human fetal liver and their origin and function await further investigation.

目的采用免疫组织化学方法标记不同发育时期人胚胎肝干细胞中巢蛋白(Nestin)的表达,探讨肝干细胞新的表面标志蛋白。方法取材不同发育时期胎儿肝脏,固定并制成石蜡切片,ABC法标记神经前体细胞的特异性表面标记Nestin在胎肝干细胞中的表达。结果汇管区周边界板处卵圆样干细胞表达Nestin阳性,Nestin阳性细胞呈单层紧密排列成管,呈鞘样包绕着早期汇管区,部分包绕着初级汇管区,随着次级汇管区的成熟,Nestin阳性卵圆样干细胞逐渐局限于赫令管周围;此外胚胎发育不同时期可见Nestin阳性单个核样细胞散布在肝实质内。结论肝脏内有Nestin表达阳性的干细胞存在,其功能、来源需要进一步深入研究。

 
图标索引 相关查询

 


 
CNKI小工具
在英文学术搜索中查有关human fetal tissue的内容
在知识搜索中查有关human fetal tissue的内容
在数字搜索中查有关human fetal tissue的内容
在概念知识元中查有关human fetal tissue的内容
在学术趋势中查有关human fetal tissue的内容
 
 

CNKI主页设CNKI翻译助手为主页 | 收藏CNKI翻译助手 | 广告服务 | 英文学术搜索
版权图标  2008 CNKI-中国知网
京ICP证040431号 互联网出版许可证 新出网证(京)字008号
北京市公安局海淀分局 备案号:110 1081725
版权图标 2008中国知网(cnki) 中国学术期刊(光盘版)电子杂志社