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   feeder cells 的翻译结果: 查询用时:0.182秒
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feeder cells
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  饲养细胞
     The best cultural condition is :Mitomycin C(25μg/ml) as a feeder cell inhibitor,0.5 or 1 NK cell cultured with feeder cells in medium containing rhIL-2 200u/ml,PHA 10μg/ml and 10%LCM.
     以丝裂霉素C(25μg/ml)作为饲养细胞抑制剂,以rhIL-2 200u/ml、PHA 10μg/ml及10%LCM培养,可获得较多的克隆和细胞数。
短句来源
     Me-thods Balb/c mice were immunized with EspA-Stx2B and the spleen cells of unimmunized Balb/c mice were used as feeder cells.
     方法以融合蛋白EspA-Stx2B作为抗原,免疫Balb/c小鼠,以未免疫的Balb/c小鼠脾细胞为饲养细胞;
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     Methods Mononuclear cells extracted from mouse spleen were treated with immunomagnetic bead for the isolation of CD3-/CD16+,CD56+ cells. After verified with flowcytometry,these NK cells were cultured with mice splenic cells(irradiated with 20Gy 60 Co gamma ray)as feeder cells and rhIL-2 as induction factor for 3 rounds(5 days each round).
     方法从小鼠脾脏提出的单个核细胞,应用免疫磁珠的方法分离CD3-/CD16+/CD56+细胞,并应用流式细胞仪鉴定后以总剂量为20Gy60Coγ射线照射后的小鼠脾细胞为饲养细胞,以重组人白介素-2(rhIL-2)为诱导因子,在体外进行NK细胞的扩增培养。
短句来源
     Objective To select an appropriate dosage of irradiation with 60 Co for preparation of feeder cells.
     目的 :选择合适的60 钴 (60 Co)照射剂量制备饲养细胞
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     Mouse Embryonic Stem Cells Derived Feeder Cells Support the Growth of Themselves
     胚胎干细胞来源的饲养细胞支持自体胚胎干细胞生长
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  饲细胞
     Recovering from frozen,the cells with feeder cells had higher proliferating power than cells without feeder cells had (t=3.007,P<0.01).
     兔角膜缘干细胞冻存复苏后平均CFE比较,有饲细胞组明显高于无饲细胞组(t=3.007,P<0.01)。
短句来源
     Limbal stem cells with feeder cells grew as clones. The mean CFE of limbal stem cells in the group with feeder cells was more than that in the group without feeder cells (t=2.982,P<0.01).
     与饲细胞共同培养的兔角膜缘干细胞形成细胞克隆团,细胞的平均CFE比较:有饲细胞组角膜缘干细胞明显高于无饲细胞组(t=2.982,P<0.01)。
短句来源
     Methods Treated with Mitomycin C, hFLP became feeder cells.
     方法 人胚肺成纤维细胞系细胞,经丝裂霉素C处理成饲细胞
短句来源
     When having co-cultivated with hFLP feeder cells for 5 passages, there was no caducity in corneal epithelia and endothelia.
     角膜上皮和内皮细胞原代培养5~7天,细胞密集,经人胚肺饲细胞共培养5代,细胞无衰老现象。
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     After feeder cells were discarded by 0.04% EDTA, the cultivated limbal stem cells were examined with immunofluorescence staining of PCNA.
     用EDTA去除饲细胞,间接免疫荧光染色检测角膜缘干细胞克隆团表达增殖细胞核抗原(PCNA)情况。
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  “feeder cells”译为未确定词的双语例句
     Studies on Differential Expression of Wnt Genes in Feeder Cells and Human Embryonic Stem Cells during Culture hES Cells in Vitro and RNAi for Wnt9a; Cloning and Priliminary Functional Study of Novel Genes Cymg1 and Rcet1 Related to Spermatozoa Development
     人类胚胎干细胞体外培养过程中Wnt基因在饲养层和人类胚胎干细胞中的差异表达及Wnt9a的RNAi研究;生精相关新基因Cymg1和Rcet1的克隆及初步功能研究
短句来源
     Results Stromal cells from human AGM region significantly supported proliferation of the TNC,CD34~+ cells,CD34~+ CD38~- cells and CFC,when compared with hFT and controls without feeder cells(P<0.05).
     结果在未加外源性造血生长因子的条件下,5株人 AGM 区基质细胞 hAGMS1~S5对制造血细胞总数、CD34~+及 CD34~+CD38~-细胞、造血细胞集落均有不同程度的扩增及维持作用,与无饲养层组、hFT 细胞组比较差异有统计学意义(P<0.05)。
短句来源
     ESCS at 0 pasages highly express Oct-4 (+++),middlely express CD71、PCNA、K19 (++),notexpress P63, and integrin-β1. The Oct-4 expression of ESCS decreased during subcultures in vitro ,and coculture with feeder cells is need to maintain the expression of Oct-4. ESCS were express K19、P63、PCNA、CD71、β1-integrin、α6-integrin.
     结果表明原代ESCs高表达 Oct-4,中度表达CD71、PCNA、K19,不表达β1-integrin、p63。 Oct-4的表达随传代次数增加而下降,且需有滋养层细胞共培养维持;
短句来源
     So far, however, most ES cell lines used in genetic manipulation have been derived from the 129/Sv strain. In this paper, we have established ES cell lines from blastocysts of the CD-1, C57BL/6J and 129/Sv×C57BL/6J mice besides 129/Sv mouse with a fibroblast cell as feeder cells, and supplemented with 1,000 unit/mL LIF in the culture medium.
     本研究通过传统的成纤维细胞饲养层法,从CD-1、129/Sv、C57BL/6J和129/Sv×C57BL/6J四种不同遗传背景的小鼠中分离得到12个ES细胞系,而从KM小鼠没有得到ES细胞系。
短句来源
     Objective To research the preparation of Swiss-3T3 cells with mitomycin C as feeder cells,examine the cloning and proliferation power of rabbit limbal stem cells.
     目的探讨丝裂霉素C处理Swiss3T3成纤维细胞作为饲细胞的条件,了解兔角膜缘干细胞克隆化培养的增殖状况。
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  feeder cells
Much attention is paid to finding the most optimal conditions for ESC cultivation, mainly to the development of cultivation techniques excluding animal feeder cells and other components of animal origin.
      
Both observations and simulation show that an extremely intense growth of the cloud was associated with its merge with feeder cells.
      
The seeding of feeder cells caused a change in the direction of the cloud movement.
      
Thus the inner cell masses of blastocysts obtained from trisomic embryos were placed on feeder cells and cultured for seven additional days, following which the resulting cell colonies were examined for chromosome content.
      
Methods for producing and cloning B-cell hybridomas using ORIGEN? Hybridoma Cloning Factor as a replacement for feeder cells are described.
      
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At first, we chose Syrian hamster embryocells and NIH/3T3 cells as target cells forstudying morphological transformation andcomparing their transforming results.Thererults showed that both kinds of cellsappea red the morphological alterationwithin 2 weeks after treatment by MCA1μg/ml or MCA 0.1 μg/m1 + TPA 0.1 μg/ml.There was no spontaneous transformationin Syrian hamster embryo cells;but therewas,in NIH/3T3 cells.We have comparedthe transforming efficiencies of positivegroups with those of negative groups inNIH/3T3...

At first, we chose Syrian hamster embryocells and NIH/3T3 cells as target cells forstudying morphological transformation andcomparing their transforming results.Thererults showed that both kinds of cellsappea red the morphological alterationwithin 2 weeks after treatment by MCA1μg/ml or MCA 0.1 μg/m1 + TPA 0.1 μg/ml.There was no spontaneous transformationin Syrian hamster embryo cells;but therewas,in NIH/3T3 cells.We have comparedthe transforming efficiencies of positivegroups with those of negative groups inNIH/3T3 cells by x~2 test, P<0.01 or P<0.05.So we suggest that NIH/3T3 cells shouldalso be used as target cells if there is nosource of Syrian hamster embryo cells.Then,we used rat embryo cells orNIH/3T3 cells as feeder layers preparedby irradiation of ~(60)Co (5000 rad) or treatmentof mitomycin C(4μg/ml 37℃,2h).Theresults of the studies showed that therewas little difference in morphologicaltranforming efficiency or clonal efficiencybetween both kinds of treatment forthe feeder cells.So we are able to studywith this method even if we have nosource of ~(60)Co or rat embryo cells.We have also compared clonal trans-forming test with transforming foci test ofNIH/3T3 cells.The former is in short-termand has spontaneous transformation;thelatter is in long-term, but its techniqueis simple.So the laboratories can chooseeither of these tests according to theirown conditions.

本文系统地研究了用哺乳动物细胞作体外短期转化试验检测致癌物和促癌物,并对几种实验条件和方法作了比较。结果表明SHE细胞和NIH/3T3细胞均为较理想的靶细胞;丝裂霉素C处理或~(60)Co照射的NIH/3T3 细胞分别作饲养层均可获得理想的检测效果。

Rat tracheal epithelial cell ( RTE) grows well in vitro under the support of 3T3 feeder cell layer. Exposure of nickel compounds results in the development of enhanced growth variant co lonies of different size and morphologies. The inducibilities of these compounds were ranked as following:Ni3S2, NiS C, NiS A, NiCl2, NiSO4, and dosely concurred with the results of animal carcingenic test. It seems that the RTE could be used as an in vitro. model for carcinogenesis studies of respiratory tract tumour...

Rat tracheal epithelial cell ( RTE) grows well in vitro under the support of 3T3 feeder cell layer. Exposure of nickel compounds results in the development of enhanced growth variant co lonies of different size and morphologies. The inducibilities of these compounds were ranked as following:Ni3S2, NiS C, NiS A, NiCl2, NiSO4, and dosely concurred with the results of animal carcingenic test. It seems that the RTE could be used as an in vitro. model for carcinogenesis studies of respiratory tract tumour and also as a quantitative system for chemical carcinogen detection.

原代RTE细胞在3 T3滋养细胞支持下,在体外培养条件下可生长。在接受MNNG与多种镍化合物作用后,可出现恶性转化。镍化合物诱发恶性转化的能力强弱依次为Ni_3S_2、Nis(C)、NiS(A)、NiCl_2和NiSO_4,与动物诱癌试验结果一致。该系统可用于研究化学物质诱发呼吸道肿瘤的致癌过程,亦可用于定量检测化学致癌物。

We established a semisolid colony culture assay for acute lymphoblastic leukemic cells in vitro. The basic principle of this technique is identical to that used successfully for normal progenitors and acute myeloblastic leukemic cells except that this technique requires feeder cells and low O_2 tension (5%). Bone marrow mononuclear cells depleted of T cells were cultured with media conditioned by T cells in the presence of phytohemagglutinin and irradiated nonadherent mononuclear cells,...

We established a semisolid colony culture assay for acute lymphoblastic leukemic cells in vitro. The basic principle of this technique is identical to that used successfully for normal progenitors and acute myeloblastic leukemic cells except that this technique requires feeder cells and low O_2 tension (5%). Bone marrow mononuclear cells depleted of T cells were cultured with media conditioned by T cells in the presence of phytohemagglutinin and irradiated nonadherent mononuclear cells, Cells in the colonies had a characteristic pattern, with same cytochemical staining and abnormal karyotype as the original leukemic marrow. The results suggest that the secondary plating efficiency as tested in recloning studies of the primary blast colonies is predictive of the clinical outcome. The assay appears to help detection of small number residual leukemic cells according to the colony pattern. We consider this technique will be valuable in predicting the sensitivity of chemotherapy and in evaluating the quality of purged marrow for autologous marrow transplantation.

本文采用PHA-TCM及饲养细胞作为刺激条件,并在低氧环境下,建立了急淋L-CFU体外半固体培养方法。22例急性淋巴细胞白血病和恶性淋巴瘤患儿骨髓细胞培养,成功率为91%。集落数与接种细胞数呈线性关系。通过事先去除成熟T淋巴细胞。并根据集落细胞形态、细胞化学染色、染色体及集落细胞传代培养等检查,证明集落细胞属急淋细胞性质。本组试验提示第二代集落得率与临床预后可能有一定关系,同时通过集落培养可协助临床鉴别诊断,并可检测骨髓细胞中白血病细胞残存,为开展自身骨髓移植和体外药敏试验提供一个临床试验的手段。

 
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