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cre gene
相关语句
  cre基因
     For specific expressing Cre recombinase in central nerve system(CNS),a transgenic construct (pGFAP-Cre-hGH),containing the β-globin insulators,1.8 kb of glial fibrillary acidic protein gene(GFAP)5′end regulation region,Cre gene and polyA of human growth hormone gene(hGH) was generated,in which the 5′end regulation region of GFAP was isolated from a 129sv mouse genomic DNA library with PCR-screening.
     利用从 12 9sv小鼠基因组文库克隆得到的 1.8kb的胶质细胞原纤维酸性蛋白 (GFAP)基因的 5′端调控序列 ,构建了含有 2个 β 珠蛋白绝缘子、GFAP 5′端调控区、Cre基因和人生长激素基因 (hGH)polyA的转基因载体pGFAP Cre hGH。
短句来源
     A chloroacetanilide-induced promoter(In5-2) was used to control the expression of Cre gene in this system.
     在该系统中,Cre基因在玉米乙酰苯胺类化合物诱导启动子(In5-2)的控制下表达。
短句来源
     Expression of cre Gene in Escherichia coli and Bioassay Its Expression Product
     cre基因在大肠杆菌中的表达及表达蛋白活性的检测
短句来源
     The Cre gene was cloned from lysogen BM25.8 by PCR. The Cre gene was then inserted in between the CaMV35S-35S double promtor and Nos terminator of PBI525 plasmid after sequencing. The expression box was cut down and inserted into plasmid pBINPLUS to obtain the final fertility-restoring expression vector pBINPLUSCre.
     PCR方法从溶原菌BM25.8基因组DNA扩增出Cre基因,克隆测序验证无误后插入PBI525 CaMV35S-35S双启动子和Nos终止子之间,再将表达盒切下插入pBINPLUS质粒相应位点,得到恢复基因植物表达载体pBINPLUSCre。
短句来源
     The Cre gene was cloned from lysogen BM 25.8 with the PCR procedure. The Cre gene was then inserted in between the CaMV 35 S-35 S double promoter and nos terminator of PBI 525 plasmid after sequencing. The expression box was cut down and inserted into plasmid pBINPLUS to obtain the final fertility-restoring gene expression vector pBINPLUSCre.
     PCR方法从溶原菌BM25.8基因组DNA扩增出Cre基因,克隆测序验证无误后插入PBI525CaMV35S-35S双启动子和Nos终止子之间,再将表达盒切下插入pBINPLUS质粒相应位点,得到恢复基因植物表达载体pBINPLUSCre。
短句来源
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  “cre gene”译为未确定词的双语例句
     A chloroacetanilide-induced promoter (In5-2) was used to control the expression of Cre gene in this system.
     在该系统中,乙酰苯胺诱导启动子(In5-2)控制Cre基因的表达。
短句来源
     11 founder mice carrying the Cre gene were identified by PCR and Southern blot.
     经PCR和Southern检测鉴定出11只在基因组上整合有Cre重组酶基因的小鼠。
短句来源
     This plasmid was transferred into Escherichia coli BL21(DE3). The Cre gene was overexpressed and then induced by IPTG.
     将此质粒导入大肠杆菌BL21(DE3)中进行IPTG诱导的Cre基因的高效表达.
短句来源
     Most of pollen in T1 generation transgenic lines with barnase and Cre gene were sterile after heat shock treatment.
     同时整合Cre重组酶基因和Barnase基因的部分T_1代转基因植株经过热激诱导后大部分花粉败育。
短句来源
     The binary vector containing Cre gene under control of Gmhspl7.5C promoter and a blocking fragment franked by two loxp sites between promoter Osg6B and barnase gene was constructed.
     构建了包含大豆热激启动子Gmhsp17.5C调控Cre重组酶基因,及水稻花药绒毡层特异性启动子Osg6B与Barnase基因之间带有阻断序列(两侧是loxp位点)的双元载体。
短句来源
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  相似匹配句对
     The integration of Cre recombinase gene was detected using PCR.
     利用PCR检测子代小鼠Cre重组酶整合情况。
短句来源
     Cre gene was linked to the intron of human growth factor gene.
     同时 ,在Cre基因 3′端添加了含内含子的 3′端人生长激素基因。
短句来源
     As to the RYR gene.
     长白猪群中,未发现有该基因存在。
短句来源
     -casein gene.
     -酪蛋白基因5'侧序列。
短句来源
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  cre gene
In 20% of the regenerants bar gene excision was due to the expression of stably integrated cre gene, whereas in 14% of plants site-specific recombination was a consequence of transient cre expression.
      
The plant intron-containing cre gene, creINT, was configured in such a way that self-excision generated an intact hygromycin resistance selectable marker gene.
      
To study the impact of different DNA configurations on the stability of transgene expression, a variant of the cre gene was developed.
      
Spatial and temporal expression of the Cre gene under the control of the MMTV-LTR in different lines of transgenic mice
      
Homozygous VCAMflox/flox mice were mated to transgenic lines of mice expressing the cre gene under control of the murine platelet endothelial cell adhesion molecule-1 (PECAM-1) promoter.
      
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To generate the transgenic mice expressing cyclization recombination enzyme, the recombinant gene, in which the coding region of cre gene is drived by the promoter of mouse Mx gene, was microinjected into pronuclei of fertilized mouse eggs. Founders of transgenic mice harbouring the recombinant gene were screened by polymerase chain reaction (PCR) at genomic DNA level and confirmed by Southern blot. One line of Mx-cre transgenic mice was obtained. Then, the Mx-cre transgenic...

To generate the transgenic mice expressing cyclization recombination enzyme, the recombinant gene, in which the coding region of cre gene is drived by the promoter of mouse Mx gene, was microinjected into pronuclei of fertilized mouse eggs. Founders of transgenic mice harbouring the recombinant gene were screened by polymerase chain reaction (PCR) at genomic DNA level and confirmed by Southern blot. One line of Mx-cre transgenic mice was obtained. Then, the Mx-cre transgenic mouse line was cultured and propagated.

将携带MX启动子调控的Cre基因真核表达载体pMx-cre线性化后,通过受精卵显微注射途径制备转基因小鼠。共注射99个卵,产仔20只,利用PCR对小鼠进行筛选,以基因组Southern blot确证,最后得到一个阳性的小鼠品系,进而将其保种和扩大繁殖。

The Cre recombinase and its activity in C57-TgN(Mx-Cre) transgenic mice is studied by polymerase chain reaction (PCR), Western blot, immunohistochemistry, immunogold electron microscopy and Southern blot. C57-TgN(Mx-Cre) transgenic mice harbouring cre gene in genomic DNA is demonstrated by PCR, and these mice which are induced by INF- αlb could express Cre recombinase, which is confirmed by Western blot. With immunohistochemistry, we find that the Cre recombinase expresses in hepatocyte...

The Cre recombinase and its activity in C57-TgN(Mx-Cre) transgenic mice is studied by polymerase chain reaction (PCR), Western blot, immunohistochemistry, immunogold electron microscopy and Southern blot. C57-TgN(Mx-Cre) transgenic mice harbouring cre gene in genomic DNA is demonstrated by PCR, and these mice which are induced by INF- αlb could express Cre recombinase, which is confirmed by Western blot. With immunohistochemistry, we find that the Cre recombinase expresses in hepatocyte cytoplasm and nuclear of C57-TgN(Mx-Cre) transgenic mice. Cre recombinase expressed in hepatocyte cytoplasm and nuclear is further confirmed by immunogold electon microscopy. And it is supported that the Cre recombinase which is created from C57-TgN(Mx-Cre) transgenic mice induced by INF-αld b can direct DNA recombination reaction in vitro. All evidence leads us supporting the view that the Cre recombinase expressed in C57- TgN(Mx-Cre) transgenic mice has activity. Thus we find a method to detect the activity of Cre recombinase in vitro.

利用PCR、Western blot、免疫组化、免疫金标电镜、Southern blot从DNA水平、蛋白水平分析干扰素诱导后Mx-Cre转基因小鼠肝组织中Cre重组酶的表达及其表达产物的活性。在对Mx-cre转基因小鼠基因组中整合有cre基因进行确定后,通过干扰素诱导Mx-cre转基因小鼠表达Cre重组酶,结果表明转基因小鼠肝细胞核和细胞质中均有Cre重组酶的表达,并在超微水平进一步证实。将含表达的Cre重组酶的肝细胞核抽提液加入到带有loxP位点的DNA中进行重组,分析证明Mx-Cre转基因小鼠表达的Cre重组酶具有重组活性,从而建立了体外检测Mx-Cre转基因小鼠表达的Cre重组酶活性的方法。

Objective: To observe the multipotent differentiation ability of bone marrow cells in irradiated syngeneic recipient mice. Methods: Marrow cells isolated from male C57-TgN (Mx-Cre) mice were transplanted intravenously into irradiated syngeneic female mice to reconstruct their marrow system. The expressions of 2 markers of donor cells, sry and Mx-cre gene, were detected by PCR and immunohistochemistry in several organs of recipient mice. Results: Both sry and Mx-cre were positive by PCR detection...

Objective: To observe the multipotent differentiation ability of bone marrow cells in irradiated syngeneic recipient mice. Methods: Marrow cells isolated from male C57-TgN (Mx-Cre) mice were transplanted intravenously into irradiated syngeneic female mice to reconstruct their marrow system. The expressions of 2 markers of donor cells, sry and Mx-cre gene, were detected by PCR and immunohistochemistry in several organs of recipient mice. Results: Both sry and Mx-cre were positive by PCR detection in liver, kidney, skin and spleen of recipient mice. Immunohistochemistry outcomings were coincident with those of PCR. Conclusion: The results above suggest that marrow cells can not only reconstruct the hematopoiesis system of irradiated syngeneic mice, but also show multipotency of differntiating into histocytes of liver, kidney, skin and spleen.

目的:探讨骨髓细胞在受致死剂量照射的受体鼠体内的多向分化潜能。方法:将C57-TgN(Mx-cre)雄性小鼠的骨髓细胞经尾静脉植入受致死剂量照射的雌性受体鼠,重建受体鼠骨髓,然后分别用PCR和免疫组化分析检测受体鼠的多个器官组织中sry及Mx-cre基因的分布及表达情况。结果:PCR和免疫组化分析结果均显示,供体细胞的两个标志sry及Mx-cre在受体鼠的肝脏、肾脏、皮肤、脾脏等多个器官中都有表达。结论:骨髓细胞不仅可以重建受致死剂量照射受体鼠的造血系统,而且具有分化为肝脏、肾脏、皮肤、脾脏等组织细胞类型的多向潜能性。

 
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