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viral vector
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  病毒载体
     ③Eighteen hours after tumor cell inoculation into nude mice, the subcutaneous MG-2F11 tumors were infiltrated by more macrophages than those of the MG-VV (U251MG cell only transfected with viral vector) tumors.
     ③接种瘤细胞到裸小鼠皮下18 h后,侵入MG-2F11肿瘤的巨噬细胞明显多于侵入MG-VV(仅转染病毒载体的U251MG细胞)肿瘤的巨噬细胞;
短句来源
     Construction of Esptcin-Bars viral vector with hepatitis B virus pre C/C gene and itsexpression in HepG2 cells
     HBV前C/C基因EB病毒载体的构建及在HepG2细胞中的表达
短句来源
     To investigate the non viral vector mediating human coagulation factor Ⅷ gene expression in mouse 32D cell line, a recombinant plasmid vector, pRC/RSV hFⅧBDcDNA, was constructed by cloning B domain deleted (△760aa-1639aa) human factor Ⅷ cDNA (hFⅧBDcDNA) into plasmid vector, pRC/RSV. The plasmid RC/RSV hFⅧBDcDNA was then transfected by means of SuperFect Transfection Reagent into mouse 32D cell line.
     为了观察非病毒载体 pRC/RSV介导的人凝血因子Ⅷ基因在小鼠 32D细胞系中的表达 ,将B结构域缺失(△ 76 0aa - 16 39aa)的人FⅧcDNA(hFⅧBDcDNA)亚克隆至质粒载体pRC/RSV ,构建重组质粒载体 pRC/RSV hFⅧBDcDNA。
短句来源
     Results :h N G Fc D N Awas cloned correctly into retro viral vector p L X S N. Recombinant retroviral vector p L N G F S N was constructed .
     结果:h N G Fc D N A已正确地克隆到逆转录病毒载体p L X S N 中,而构建成重组逆转录病毒载体p L N G F S N。
短句来源
     A clone containing signal sequence of prM,coding sequences o f prM,E,NS1 and NS2a proteins of J apanese encephalitis virus(JEV)GSS strain was constructed. Along with that,a recombinant vaccinia-JEV vi rus NTVA2427(E/L)2 JEV containing the target genes mentioned above w as also constructed by homologous recombination of the clone with a viral vector of non-replicative va ccinia virus(NTV).
     克隆流行性乙型脑炎(乙脑)病毒野毒(JEV)GSS株前膜蛋白信号序列、前膜蛋白(prM)、包膜蛋白(E)、非结构蛋白-1(NS1)和非结构蛋白NS2a的编码基因,并与非复制型痘苗病毒载体NTV进行同源重组,构建了乙脑病毒非复制型重组痘苗病毒疫苗株NTVA(E/L)JEV。
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  “viral vector”译为未确定词的双语例句
     Construction of an adeno-associated viral vector serotype 2/1 containing human interleukin-10 and its expression in donor liver
     人白介素10重组腺相关病毒血清型2/1杂合载体的构建及转导供肝的效果
短句来源
     AIM: To construct an adeno-associated viral vector sero-type 2/1 (AAV2/1) containing human interteukin-10 (hIL-10) gene and to observe its expression in donor liver.
     目的:构建含人白介素10(hIL-10)的腺相关病毒血清型2/1杂合载体(AAV2/1),并观察其在供肝移植物中的表达.
短句来源
     Part one: Experimental study of breast cancer treatment with recombinant EGFR antisense adenovirusHuman EGFR cDNA fragment was subcloned in the opposite orientation to the cytomegaloviral promoter and inserted into a E1/E3-deleted type 5 adeno viral vector to obtain AdE5 construct which expresses EGFR antisense RNA.
     一、EGFR反义重组腺病毒治疗乳腺癌的动物实验研究人EGFR的cDNA片段被反向亚克隆入E1/E3缺失的5型腺病毒载体中而得到AdE5,EGFR反义RNA的表达由CMV启动子控制;
短句来源
     The SRSV antigenic protein was expressed in the broad bean plants by Potyvirus vector pClYVV/CP/W. The full-length sequence of 1605 bp for SRSV was inserted between nuclear inclusion b(NIb) and coat protein(CP) of infectious viral vector pClYVV/CP/W derived from clover yellow vein virus(ClYVV) of potyvirus. The recombinant plasmids were constructed and named pClYVV-NV1.6,and then they were mechanically inoculated in broad bean seedlings.
     将引起人类腹泻主要病原物小球状病毒(Small round structured virus;SRSV)编码抗原蛋白基因的全长序列1605bp,导入来自三叶草黄脉病毒(Clover yellow vein virus;ClYVV)侵染性全长cDNA克隆的侵染性植物病毒表达载体pClYVV/CP/W基因组的NIb/CP基因之间,构建了重组病毒克隆pClYVV-NV1.6。
短句来源
     Effect of Hypoxia on Expression of Human VEGF_(165) Gene Transferred by Recombinant Adeno-associated Viral Vector2 in Rat Cardiomyocytes in Vitro
     低氧对rAAV-2介导的hVEGF165基因在大鼠心肌细胞中表达的影响
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  相似匹配句对
     Gene Therapy by Viral Vector
     运用病毒载体实现基因治疗
短句来源
     The Viral Vector System of Gene Therapy
     基因治疗的病毒载体系统
短句来源
     INTELLIGENT VECTOR
     智能向量
短句来源
     puro vector.
     puro上。
短句来源
     Athletes and Viral Myocarditis
     运动员与病毒性心肌炎
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  viral vector
Additionally, baculovirus ability to transduce target cells was compared to that of an adenoviral vector, a well characterized and widely used viral vector.
      
The BLV E was inserted into a non-viral vector, pLacZ, in order to determine if packaging of the non-viral vector RNA would occur.
      
A viral vector was constructed from pEALSR2 by creating artificial protease processing sites by duplicating the Q/G protease cleavage site between 42KP and Vp25.
      
benthamiana was transformed with a nontranslatable portion of a TMV viral vector including part of the replicase gene, the movement protein gene, a gene for green fluorescent protein (GFP), and the coat protein gene.
      
The evolution of this region and its potential use in the development of a viral vector system are discussed.
      
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Neuroblastoma is one of the most common pediatric solid tumors. In approximately 50% of clinically advanced cases N-myc oncogene is amplified. Nerve gro-wth factor (NGF)is capable to induce differentiation of some neuroblastoma cell lines. But the cerl lines with amplified N-myc usually failed to respond to NGF. It may be due to the shortage of NGF receptors in these cell lines. The amplification of N-myc oncogene may also be responsible. We introduced the recombinant NGF receptor gene into the human neuroblastoma...

Neuroblastoma is one of the most common pediatric solid tumors. In approximately 50% of clinically advanced cases N-myc oncogene is amplified. Nerve gro-wth factor (NGF)is capable to induce differentiation of some neuroblastoma cell lines. But the cerl lines with amplified N-myc usually failed to respond to NGF. It may be due to the shortage of NGF receptors in these cell lines. The amplification of N-myc oncogene may also be responsible. We introduced the recombinant NGF receptor gene into the human neuroblastoma cell line (IMR-32),which was lack of NGF receptor and had N-myc amplification. The trans-formant line (IMR-32/NGFR) expressed NGF receptor both in mRNA and receptor protein levels. The parent line (IMR-32) and the control transformant line (IMR-32/NEO) , obtained by infection with empty viral vector were all negative for NGFR expression in mRNA and receptor protein levels as shown by Northern Blotting and Flow Cytometry using fluorescent labeled monoclonal antibodies against NGFR. After NGF treatment, transformant cell line (IMR-32/NGFR) did not show significant change in morphology. The expression of neurofilament light chain mRNA only increased slightly. No significant alteration was found in the expression of "N-myc and K-ras oncogenes among IMR-32, IMR-32/NEO and IMR-32/NGFR before and after NGF treatment. These results indicate the amplification of N-myc oncogene may still limit NGF responce in this cell line even after the restoration of NGF receptor.

神经母细胞瘤是儿童期的常见恶性肿瘤之一。临床上,半数以上神经母细胞瘤有明显的N-myc癌基因扩增。神经生长因子(NGF)可使某些神经母细胞瘤细胞分化成神经元样的细胞。但对有N-myc扩增的神经母细胞瘤则可能因NGF受体缺乏而无明显促分化反应。本研究运用重组DNA技术将人NGF受体基因重组到有N-myc扩增,且NGF受体表达很低的神经母细胞瘤系(IMR-32)中,经克隆化培养、筛选,建立了稳定的细胞系—IMR-32/NGFR和对照细胞系IMR-32/NEO。经用抗NGFR的单克隆抗体检测用Flow cytometry技术证实IMR-32/NGFR系中有明显的NGF受体表达,而其母系IMR-32和空病毒对照系IMR-32/NEO则无表达迹象,说明NGF受体表达在IMR-32/NGFR系中是特异的,并能同抗NGFR单克隆抗体特异结合。用Northern B10ting技术亦测得IMR-32/NGFR中NGFR的mRN A明显表达。而其母系IMR-32和对照系IMR-32/NEO则无明显表达。这说明IMR-32/NGFR系中NGFR在mRNA水平上亦是特异的。N-myc和K-ras癌基因在IMR-32、IMR-32/...

神经母细胞瘤是儿童期的常见恶性肿瘤之一。临床上,半数以上神经母细胞瘤有明显的N-myc癌基因扩增。神经生长因子(NGF)可使某些神经母细胞瘤细胞分化成神经元样的细胞。但对有N-myc扩增的神经母细胞瘤则可能因NGF受体缺乏而无明显促分化反应。本研究运用重组DNA技术将人NGF受体基因重组到有N-myc扩增,且NGF受体表达很低的神经母细胞瘤系(IMR-32)中,经克隆化培养、筛选,建立了稳定的细胞系—IMR-32/NGFR和对照细胞系IMR-32/NEO。经用抗NGFR的单克隆抗体检测用Flow cytometry技术证实IMR-32/NGFR系中有明显的NGF受体表达,而其母系IMR-32和空病毒对照系IMR-32/NEO则无表达迹象,说明NGF受体表达在IMR-32/NGFR系中是特异的,并能同抗NGFR单克隆抗体特异结合。用Northern B10ting技术亦测得IMR-32/NGFR中NGFR的mRN A明显表达。而其母系IMR-32和对照系IMR-32/NEO则无明显表达。这说明IMR-32/NGFR系中NGFR在mRNA水平上亦是特异的。N-myc和K-ras癌基因在IMR-32、IMR-32/NEO、IMR-32/NGFR三系中无明显变化。在NGF处理后,形态上三系均无明显的分化迹象。但IMR-32/NGFR在神经原纤维轻链的表达上轻度增高。c-fos癌基因的表达见于所有三个细胞系,IMR-32/NGFR略强些。这些说明,在恢复了NGFR基因和表达之后,对N-myc和K-ra

Human leukocyte differentiation antigens CD4 and CD19 were transfected into Cos cell line by electroporation. Indirect immunofluorescence and APAAP immunocytochemical staining shows that transient expression and high efficiency transformation of the two genes were obtained,we used pNTK-EL-2R,a retro-viral vector containing the human IL-2 receptor gene (CD25),to transfect amphotropic and ecotropic virus packing cell lines, subsequently .cell-free culture supernatant was used to infect murine fibroblast...

Human leukocyte differentiation antigens CD4 and CD19 were transfected into Cos cell line by electroporation. Indirect immunofluorescence and APAAP immunocytochemical staining shows that transient expression and high efficiency transformation of the two genes were obtained,we used pNTK-EL-2R,a retro-viral vector containing the human IL-2 receptor gene (CD25),to transfect amphotropic and ecotropic virus packing cell lines, subsequently .cell-free culture supernatant was used to infect murine fibroblast NTH3T3.. The liter of virus generated from virus-producing cells about 3. 3X 10s CPU/ml. IL-2R mRNA expression of transfected cells was assayed with Northern blot. Expression of the neo gene in retraviral vector was shown by PCR. These date demonstrate efficient transfer and expression of human IL-2R gene. The research provide a new approach for study of molecular structure and function of antigens,and of idendification and screening of McAbs.

本文应用电转移法将CD4、CD19抗原基因转入Cos7细胞获得高效转移和暂短表达,检测96h抗原阳性频率分别为3.7×10~(-3)和2.6×10~(-3)。将含有人IL-2RcDNA(CD25)的逆转录病毒载体pN-TK-IL-2R转染PA317和2病毒包装细胞系,收集病毒上清感染NIH3T3细胞;病毒滴度可至3.3×10~5CFU/ml,细胞传代40次间接免疫荧光及APAAP法均可见阳性转染细胞;RNA点杂交检测转染细胞有IL-2R基因mRNA转录产物表达。应用PCR方法扩增出逆转录病毒载体上选择标记基因(Neo),结果表明已获得稳定表达人IL-2R基因的转染细胞株。上述基因的转移和表达为有关抗原功能研究、鉴定及筛选相应单克隆抗体提供了有效的途径。

In order to discovery and study new cDNA of human gene, a DXFD_(52)-related human hepatocyte cDNA(DE)is sequenced partially with T7 DNA polymerase, Then we search the EMBL(European Molecular Biology Laberato-ry) for homology to the sequenced parts of DE. we can not find any homologous human gene or DNA (or cDNA)frag-ment in the library. So we consider that DE may be a new human gene cDNA fragment. We also insert DE into the retro-viral vector pLXSN successfully.which makes it possible to study the function...

In order to discovery and study new cDNA of human gene, a DXFD_(52)-related human hepatocyte cDNA(DE)is sequenced partially with T7 DNA polymerase, Then we search the EMBL(European Molecular Biology Laberato-ry) for homology to the sequenced parts of DE. we can not find any homologous human gene or DNA (or cDNA)frag-ment in the library. So we consider that DE may be a new human gene cDNA fragment. We also insert DE into the retro-viral vector pLXSN successfully.which makes it possible to study the function of the cDNA.

为了寻找和研究新的人类基因cDNA,本实验以T7DNA聚合酶对—DXFD_(52)相关人肝细胞cDNA(DE)进行了分段部分测序,并将所测各部分序列分别在EMBL(欧洲分子生物学库)中进行核酸同源性检索,结果在库中没有找到任何具有同源性的人类基因或DNA(或cDNA)片段。因此,我们初步认为DE为一新的人类基因cDNA片段。同时为初步探讨DE的功能,我们还成功地将DE构建于反转录病毒载体pLXSN上。

 
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