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institute of pediatrics
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  儿科研究所
     METHODS: Totally 1 106 children (763 boys and 343 girls) with hyperkinetic syndrome, who were treated in the Department of Psychology and Intelligence, Children's Hospital affiliated to Capital Institute of Pediatrics between October 2001 and October 2004, were divided into 3, 4, 5 and 6-year-old group (n=220, 319, 280 and 287 respectively).
     方法:以2001-10/2004-10就诊于首都儿科研究所附属儿童医院心理智力门诊的多动儿童1106人为观察对象,男763人,女343人。 按照年龄3,4,5,6岁分组,每组人数分别为220,319,280,287人。
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     METHODS: The experiment was carried out in the Institute of Pediatrics, Renmin Hospital, Wuhan University from August to November 2005. The 56 BALB/ c mice were randomly divided into 3 groups: Group A was normal control group with 8 mice (did not received any dispose).
     方法:实验于2005-08/2005-11在武汉大学人民医院儿科研究所进行。 将56只健康BALB/c小鼠随机分为3组:正常组:8只小鼠不做处理;
短句来源
     Methods During the influenza outbreak in Beijing area of 1998-1999, specimens were collected from the Capital Institute of Pediatrics and the Beijing Epidemic Prevention Station.
     方法 在 1998年冬北京地区流感病毒暴发流行期间 ,于首都儿科研究所附属医院门诊及北京市防疫站采集标本进行病毒分离、鉴定 ,并对其中 3株病毒的血凝素重链区 (HA1)进行了核苷酸和氨基酸序列分析。
短句来源
     Totally 710 serum specimens collected from non-respiratory infection patients visited the Outpatient Departments of Children′s Hospital affiliated to Capital Institute of Pediatrics and Xuanwu Hospital, Beijing from April 1996 to March 1997 were tested for specific IgG antibody against hMPV N protein.
     随后对1996年4月至1997年3月自首都儿科研究所附属儿童医院和北京宣武医院采集的门诊非呼吸道感染患者及正常儿童的体检血清710例进行hMPVN蛋白特异性IgG抗体检测。
短句来源
     SETTING: Department of Endocrinology, Children's Hospital Affiliated to Shanghai Jiaotong UniversityMATERIALS: The experiment was performed in Institute of Pediatrics of Children's Hospital Affiliated to Shanghai Fudan University from October 2001 to April 2002, in which, 24 SD white rats were employed, of purebred, female and clean grade.
     单位:上海交通大学附属儿童医院内分泌科。 材料:实验于2001-10/2002-04在上海复旦大学附属儿科医院儿科研究所完成。
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  “institute of pediatrics”译为未确定词的双语例句
     SETTING and PARTICIPANTS:A total of 79 infants(39 females and 40 males,mean age was 39.3 weeks old,mean mass was 3 516 g) were recruited from Xinmi Maternal and Child Health Hospital of Henan from May 1999 to December 2000.The samples of plasma were measured in the Beijing Medical University and Capital Institute of Pediatrics to detect the content of vitamin K in plasma and the level of protein induced by vitamin K absence,respectively.
     地点和对象:由河南省妇幼保健院负责收集1999-05/2000-12在新密市妇幼保健院出生的79例(男40例,女39例;平均胎龄39.3周;平均体质量3516g)婴儿血浆,送至北京医科大学和首都儿研所分别检测血浆中维生素K含量和维生素K缺乏诱导蛋白的水平。
短句来源
     Methods Eighty five specimens of nasopharyngeal aspirates from infants with bronchiolitis admitted to the Affiliated Children′s Hospital of the Capital Institute of Pediatrics during the period of the winter of 1995 through the spring of 1997 were cultured for viruses, and RSV antigen in the nasopharyngeal cells was also detected, followed by subgroup identification of RSV positive isolates by using monoclonal antibodies and indirect immunofluorescent technique.
     方法(1)对1995年冬~1997年春住院的85例临床拟诊为毛细支气管炎的患儿进行鼻咽分泌物病毒培养、抗原检测及RSV阳性分离株的单克隆抗体间接免疫荧光法亚型鉴定;
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  相似匹配句对
     ON INSTITUTE
     论学会
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     Institute Transformation
     改革正未有穷期——山东省冶金科学研究院深化改革纪实之二
短句来源
     Suggestions on the Reform of C. E. T in P. E. Institute
     我院大学英语教学改革的思考与建议
短句来源
     On the Industrilization of Adult Institute
     成人高校产业化初探
短句来源
     The application of fructooligosaccharides in pediatrics
     低聚果糖在儿科中的应用
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  institute of pediatrics
The Institute of Pediatrics, Niloufer Hospital Hyderabad
      
The study cohort consisted of 47 patients with idiopathic MPGN Type I treated at the National Institute of Pediatrics, Mexico City, between 1971 and 2001.
      
Central nervous system atypical teratoid rhabdoid tumor: experience at the National Institute of Pediatrics, Mexico City
      
The analysis of 65 medulloblastomas in children treated at the National Institute of Pediatrics, Mexico City, between 1971 and 1986 is reported.
      
The National Institute of Pediatrics in Mexico City is struggling to reverse escalating cancer mortality among children aged 4 to 15.
      


This survey covers 1774 infants and children with acute gastroenteritis (diarrhea) in 17 provinces and cities of China.Stool specimens collected from 1774 children with diarrhea,from 98 children without diarrhea,from 135 babies without diarrhea,from 36 adults with diarrhea,and from 37 normal adults were examined by polyacrylamide gel electrophoresis (PAGE),ELISA,EM,and virus isolation.Results obatained from PAGE showed that human rotavirus RNA was detected in 691 (39%) of the 1774 stool specirnens examined;adenovirus...

This survey covers 1774 infants and children with acute gastroenteritis (diarrhea) in 17 provinces and cities of China.Stool specimens collected from 1774 children with diarrhea,from 98 children without diarrhea,from 135 babies without diarrhea,from 36 adults with diarrhea,and from 37 normal adults were examined by polyacrylamide gel electrophoresis (PAGE),ELISA,EM,and virus isolation.Results obatained from PAGE showed that human rotavirus RNA was detected in 691 (39%) of the 1774 stool specirnens examined;adenovirus DNA was detected in 26 (1.5%);Reovirus RNA was detected in 4 (0.2%):No virus was detected in the other stool specimens.According to the genome profile on PAGE,688 (99.6%) among the 691 rotavirus RNA positive belonged to group A virus,and the other 3 (0.4%) belonged to group C virus.According to the different migration of 10,11 segments on PAGE,the RNA pattern of group A rotavirus could be divided into two subpatterns:Long and short patterns.The 446 positive were in the long pattern,while the 236 positive were in the short patterns.Among the rest there were 6 appeared to be mixed types,and the remained one was uncertained.The positive rate of rotavirus,Adenovirus,and Reovirus varied not only different years,seasons.and months,but also with age of the patients,and cities,where the patients live.Results obtained from EM showed that Rotavirus was detected in 37 (10.6%) out of 349 stool specimens examined,while adenovirus in 15 (4.3%),coronavirus-like in 3 (0.9%),small round virus in 3(0.9%),and virus crystalloid in 1 (0.3%).By using different cell line in virus isolation,such as HEK,Hep-2,and HeLa,enteroviruses were isolated from 15 (6.9%) out of 216 stool specimens,and adenoviruses were isolated in 7 (3.2%).(Capital Institute of Pediatrics 100020)(Institute of Basic Medical Science,CAMS,Beijing 100005)(Institute of Virology,Chinese Academy of Preventive Medicine,Beijing 100052)

对全国17省市收集的1774例腹泻患儿,98例非腹泻儿和135例正常新生儿,以及36例腹泻和37例无腹泻成人粪标本,用不同方法研究其病毒病原。用RNA-PAGE法查1774份患儿标本,轮状病毒RNA阳性率为39%,腺病毒为1.5%,呼肠病毒为0.2%,非腹泻儿、正常新生儿以及成年人粪标本均阴性。在所检出的轮状病毒中A组病毒占99.6%,C组病毒占0.4%。A组病毒中RNA长型占64.8%,短型占34.3%,长、短混合型占0.87%。上述各病毒不同年、季度、城市、患儿不同年龄组分布不一。电镜检查的349份标本中,轮状病毒检出率为10.6%,腺病毒为4.3%,类冠状病毒0.9%,小圆病毒0.9%,病毒副结晶0.3%,同时检查同时期的24份非腹泻儿及19份成人腹泻标本阴性。行病毒分离的216份标本中,肠道病毒检出率为6.9%,已鉴定的病毒为ECHO病毒。

Objective To understand the etiological and clinical characteristics of bronchiolitis caused by respiratory syncytial virus (RSV) subgroups.Methods Eighty five specimens of nasopharyngeal aspirates from infants with bronchiolitis admitted to the Affiliated Children′s Hospital of the Capital Institute of Pediatrics during the period of the winter of 1995 through the spring of 1997 were cultured for viruses, and RSV antigen in the nasopharyngeal cells was also detected, followed by subgroup identification...

Objective To understand the etiological and clinical characteristics of bronchiolitis caused by respiratory syncytial virus (RSV) subgroups.Methods Eighty five specimens of nasopharyngeal aspirates from infants with bronchiolitis admitted to the Affiliated Children′s Hospital of the Capital Institute of Pediatrics during the period of the winter of 1995 through the spring of 1997 were cultured for viruses, and RSV antigen in the nasopharyngeal cells was also detected, followed by subgroup identification of RSV positive isolates by using monoclonal antibodies and indirect immunofluorescent technique. The clinical data of the patients diagnosed etiologically as RSV subgroup infection were analyzed.Results Twenty two RSV isolates were obtained and the positive rate was 26%; the 22 isolates were all identified as RSV subgroup A. The young infants with RSV subgroup A bronchiolitis (1~6 month of age) accounted for 82% and the male: female ratio was 4.5∶1. The patients presented a low grade fever (<38℃) and an early wheezing (2 days right after the onset) accounted for 54% and 64%, respectively. The radiological findings were patches in both lungs (77%) and hyperinflation (64%), etc. Assessed by the Lowell score system, 8 out of 22 cases were considered as severe cases.Conclusions The subtype of RSV caused bronchiolitis in infants hospitalized in our institute from the winter of 1995 through the spring of 1997 was subtype A. These data showed that the RSV subgroup A mainly attacked young infants, and a low grade fever, an early wheezing, etc. were the main characteristics of the infection.

目的了解呼吸道合胞病毒(RSV)的亚型感染情况及其所致毛细支气管炎的临床特征。方法(1)对1995年冬~1997年春住院的85例临床拟诊为毛细支气管炎的患儿进行鼻咽分泌物病毒培养、抗原检测及RSV阳性分离株的单克隆抗体间接免疫荧光法亚型鉴定;(2)分析亚型感染病例的临床资料。结果(1)共培养出RSV2株,阳性率为26%,经鉴定全部为A亚型株;(2)22例A亚型RSV毛细支气管炎中幼婴(1~6月龄)占82%,男∶女=45∶1;发热小于38℃(54%),起病2天后出现喘憋(64%),胸部X线表现为两肺野片状阴影和肺气肿影者分别占7%和64%。经临床Lowel评分,8例评为重症。结论(1)1995年冬~1997年春住院RSV毛细支气管炎的抗原亚型是A亚型;(2)A亚型RSV毛细支气管炎临床特征主要有:幼婴为多,低热和喘憋出现较早等。

Objective To determine the epidemiologic patterns of subgroups A and B and genotypes of respriatory syncytial viruses(RSV)during two uncontinuously epidemic years from 1990 to 1991 and 1997 to 1998 in Beijing. Methods Nasopharyngeal secretion(NPS) samples of RSV positive or RSV isolates tested by indirect immunofluorescenct (IIF)assay were classified into subgroups A and B.Also, isolate of RSV were divided into at least six different lineages, designated NP1-6,by restriction mapping of the N gene. Np1,3...

Objective To determine the epidemiologic patterns of subgroups A and B and genotypes of respriatory syncytial viruses(RSV)during two uncontinuously epidemic years from 1990 to 1991 and 1997 to 1998 in Beijing. Methods Nasopharyngeal secretion(NPS) samples of RSV positive or RSV isolates tested by indirect immunofluorescenct (IIF)assay were classified into subgroups A and B.Also, isolate of RSV were divided into at least six different lineages, designated NP1-6,by restriction mapping of the N gene. Np1,3 and 6 were given by subgroup B isolates, while NP2,4 and 5 were given by subgroup A isolates. Strains of subgroup A were further subdivided into six lineages SHL1-6 on the basis of the SH gene sequence. SH lineages were closely related to each other and to NP1-6. Strains of SHL1,3, and 4 were closely related and belonged to NP2, SHL2 and 6 to NP4 and SHL5 to NP5. Results Of 145 RSV NPS samples from the winter 1997 to the spring 1998, 83(57.2%)were subgroup B strains, 62(42.8%)were subgroup A strains, the rate of occurrence of subgroup A to B strains was about 1∶1.3. Two of 10 isolates of 1997-1998 offered by viral department of research institute of Pediatrics were subgroup A strains, whereas 8 were subgroup B strains, the rate of occurrence of subgroup A to B strains was 1∶4. Eight subgroup A strains of 10 isolates from the 1990-1991 epidemic were dominant, the proportion of subgroup A to B strains was 4∶1. of 10 RSV isolates in 1997-1998, all 2 subgroup A strains gave N gene fragment restriction pattern NP4, and fell into SH lineage SHL2, whereas 8 subgroup B strains all belonged to NP3. All 8 subgroup A isolates from the 1990-1991 epidemic gave pattern NP4, and fell into SHL2, while 2 subgroup B strains all belonged to NP3. The classification of subgroups A and B deduced from NP patterns was corresponded to the definition of these subgroups by monoclonal antibodies. Conclusions These observations confirmed that subgroups A and B or multiple lineages of RSV co-circulated in Beijng, but different genomic types predominated each year. Moreover, very similar viruses were isolated up to more than 5 years ago, indicating that despite apparent diversity of the subgroup A strains, the separate lineages might be relatively stable.

目的 了解北京地区 1990~ 1991年和 1997~ 1998年两个非连续的流行年中呼吸道合胞病毒(RSV)A、B亚型和基因型的流行情况。方法 用间接免疫荧光法 (IIF)检测RSV阳性鼻咽分泌物 (NPS)标本或RSV分离株 ,划分A、B亚型。根据N基因片段的限制性酶切图型将RSV分离株分成至少 6个基因型NP1- 6NP1,3和 6属于B亚型 ,NP2 4和 5属于A亚型。根据SH基因片段的核苷酸序列将A亚型分离株进一步划分为SH基因型SHL1- 6。SHL之间关系密切 ,并与NP有关。SHL1,3和 4紧密相关 ,属于NP2 ;SHL2和 6属于NP4;SHL5属于NP5。结果  1997冬季~1998年春季 145份RSVNPS标本中 ,83份 (5 7 2 % )为B亚型 ,6 2份 (4 2 8% )为A亚型 ,A∶B为 1∶1 3。1997~ 1998流行年所获的 10株RSV分离株中 ,2株为A亚型 ,8株为B亚型 ,A∶B为 1∶4。 1990~1991流行年所获的 10株毒株中 ,8株为A亚型 ,2株为B亚型 ,A∶B为 4∶1。1997~ 1998流行年分离到的 10株RSV分离株...

目的 了解北京地区 1990~ 1991年和 1997~ 1998年两个非连续的流行年中呼吸道合胞病毒(RSV)A、B亚型和基因型的流行情况。方法 用间接免疫荧光法 (IIF)检测RSV阳性鼻咽分泌物 (NPS)标本或RSV分离株 ,划分A、B亚型。根据N基因片段的限制性酶切图型将RSV分离株分成至少 6个基因型NP1- 6NP1,3和 6属于B亚型 ,NP2 4和 5属于A亚型。根据SH基因片段的核苷酸序列将A亚型分离株进一步划分为SH基因型SHL1- 6。SHL之间关系密切 ,并与NP有关。SHL1,3和 4紧密相关 ,属于NP2 ;SHL2和 6属于NP4;SHL5属于NP5。结果  1997冬季~1998年春季 145份RSVNPS标本中 ,83份 (5 7 2 % )为B亚型 ,6 2份 (4 2 8% )为A亚型 ,A∶B为 1∶1 3。1997~ 1998流行年所获的 10株RSV分离株中 ,2株为A亚型 ,8株为B亚型 ,A∶B为 1∶4。 1990~1991流行年所获的 10株毒株中 ,8株为A亚型 ,2株为B亚型 ,A∶B为 4∶1。1997~ 1998流行年分离到的 10株RSV分离株中 ,2株A亚型均属NP4,SHL2 ;而 2株B亚型均为NP3。根据NP推导出的A、B亚型划分与单克隆抗体定义的亚型一致。结论 北京地区RSVA、B亚型或多个基因型可同时流行 ,但每个流行年的优势基因型可不同。相距 5年仍可分离到相同的毒株 ,表明尽管A亚型毒株之间存在明显的差异性 ,一些基因型仍

 
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