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cofactor     
相关语句
  辅因子
     min-1. mg-1. Co2+, Ni2+, Cd2+ could partially replace Mn2+ as a metal cofactor, theactivitieswith these ions were 17%, 14% and 11 % of the controlwith Mn2+, respectively.
     Co2+、Ni2+、Cd2+可部分代替Mn2+作为金属辅因子,活力分别为对照的17%,14%和11%。
短句来源
     With androsterone as the substrate and NAD + and thio NAD + as the cofactor, the optimum pH for the enzymatic reaction at 25℃ was 10 5 and 9 4 respectively.
     2 5℃以雄酮为底物 ,以NAD+ 和thio NAD+ 为辅因子时 ,融合蛋白参与酶促反应的最适pH分别为 1 0 5和 9 4。
短句来源
     Configuration Simulation of the Fe-Mo Cofactor in Nitrogenase——Application of Molecular Mechanicsin Molecular Model Design
     固氮酶Fe-Mo辅因子空间构型模拟——分子力学在分子模型设计中的应用
短句来源
     Methods Tissue factor pathway inhibitor (TFPI ),thrombomodulin(TM)and protein C (PC)were measured by ELISA, antithrombin Ⅲ (ATⅢ)and heparin cofactor Ⅱ(HCⅡ) by colorimetric assay.
     方法 组织因子途径抑制物 (TFPI)、凝血酶调节蛋白 (TM )和蛋白C(PC)采用ELISA法测定抗原浓度 ,抗凝血酶Ⅲ(ATⅢ )和肝素辅因子Ⅱ (HCⅡ )采用发色底物法测定活性量。
短句来源
     Conclusion: As the cofactor of HOXA10, the regular expression of Meis1 in human normal endometrium plays animportant role in HOXA10 mediated embryonic implantation.
     结论:Meis1作为HOXA10的辅因子,在人正常子宫内膜中规律性的表达,可能对HOXA10介导的胚胎着床发挥着重要作用。
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  余因子
     Study on Extraction of Stress-Induced Interface Traps in MOSFETs by Linear Cofactor Differernce Subthreshold Voltage Peak Technique
     线性余因子差分亚阈电压峰技术测量电应力诱生MOSFET界面陷阱的研究
短句来源
     The cofactor system and a criterion on common factor with given arbitrary degree
     余因子系与公因子分次判别准则
短句来源
     This paper gives a fast algorithm based on the concepts of cofactor, Shannon expansion and unate function to recursively and quickly judge if a given cube array of logical function is a tautology.
     本文给出一种高效的永真式判定算法,该算法利用余因子、Shannon展开式和单边函数,对逻辑函数的多维体列阵进行快速有效的递归判定。
短句来源
     A new experimental technique namely the linear cofactor difference sub-threshold voltage method is presented in this paper for extraction of the MOSFET interface traps induced by the gate oxide stress test and verified on N-MOSFETs.
     本文提出了用线性余因子差分亚阈电压峰测量电应力诱生MOSFET界面陷阱的新技术并进行了实验验证 .
短句来源
  余子式
     The Features and Application of the Sum of the Cofactor of a Determinant
     代数余子式之和的性质及应用
短句来源
     THE k ORDER COMPOUND ADJOINT MATRIX AND k ORDER COFACTOR MATRIX
     k阶复合伴随矩阵与k阶余子式矩阵
短句来源
     Generalization of the Cramer Rule with the Algebraic Cofactor
     用代数余子式推广cramer法则
短句来源
     Calculation of the Eigenvector of a Square Matrix by the Expanding Cofactor
     计算方阵特征向量余子式展开法
短句来源
     The basic idea of the algorithm is that both first-order and second-order cofactors can be obtained by computing the first-order cofactor of the augmented indefinite-admittance matrix or finding all the directed trees in the augmented diagraph.
     这个算法的基本思想是通过计算增广不定导纳矩阵的一阶余子式或找增广伴随有向图的全部有向树来获得所需要的一阶和二阶余子式
短句来源
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  协同因子
     Isolation,purification and identification of cofactor β_2-GPI
     协同因子β_2-GPI的分离、纯化和鉴定
短句来源
     APC,in the presence of cofactor called Protein S(PS), exerts an anticoagulant action.
     在一种叫做蛋白S(PS)的APC协同因子存在时,APC发挥抗凝活性;
短句来源
     These results suggest that MK-447 may selectively enhance the prostaglandin I2 (PGI2) level by acting as a tryptophan-like cofactor of PG endoperoxide synthetase.
     研究结果提示,MK-447很可能是通过作为前列腺素酯氧酶色氨酸样协同因子,从而发挥选择性增加PGI_2生成的作用。
短句来源
     ON MOLYBDENUM COFACTOR GENE LOCI OF VERTICILLIUM DAHLIAE
     大丽轮枝菌的含钼协同因子基因位点
短句来源
     Subsequent studies have demonstrated that eIF5A is more than an initiator of protein translation. The finding that eIF5A is a cellular cofactor of HIV-1 Rev and HTLV-1 Rex transactivator proteins in mRNA export suggests that it can have additional activities.
     随后的研究进一步发现,eIF5A还是HIV-1反式激活蛋白Rev发挥作用所必需的胞内协同因子,并参与mRNA的胞核外运,表明它还有其它的功能。
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      cofactor
    Stability of Glucose 6-Phosphate Dehydrogenase Complexed with Its Substrate or Cofactor in Aqueous and Micellar Environment
          
    The review also summarizes recent data on plasminogen activator from the vampire bat Desmodus rotundus and a role of fibrin as its cofactor.
          
    Pyridoxal 5"-Phosphate as a Catalytic and Conformational Cofactor of Muscle Glycogen Phosphorylase b
          
    This review summarizes data on structure of muscle glycogen phosphorylase b and the role of the cofactor pyridoxal 5"-phosphate in catalysis and stabilizing the native conformation of the enzyme.
          
    The affinity to the cofactor thiamine diphosphate and to xylulose-5-phosphate (donor substrate) did not change for the low molecular weight complex, while the time of binding to calcium increased.
          
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    It was discovered by Mehler in 1951 that in the presence of excess catalase and ethanol, oxygen is consumed by the chloroplast preparation upon illumination and a stoichiometrical amount of CH_3CHO is formed at the same time. This reaction is generally referred as"the Mehler reaction" and is a variation of the Hill reaction with molecular oxygen as oxidant. Recent investigations have shown that in the Vit. K or FMN-mediated photophosphorylation, ATP formation is accompanied by ~(18)O exchange. Hence, an intimate...

    It was discovered by Mehler in 1951 that in the presence of excess catalase and ethanol, oxygen is consumed by the chloroplast preparation upon illumination and a stoichiometrical amount of CH_3CHO is formed at the same time. This reaction is generally referred as"the Mehler reaction" and is a variation of the Hill reaction with molecular oxygen as oxidant. Recent investigations have shown that in the Vit. K or FMN-mediated photophosphorylation, ATP formation is accompanied by ~(18)O exchange. Hence, an intimate connection may exist between these two separately discovered reactions of isolated chloroplasts.In the present paper the activities of the Mehler reaction and photophosphorylation of isolated wheat-seedling chloroplasts were simultaneously determined. The principal results were as follows:(1) Among the catalytic cofactors of photophosphorylation, Vit. K and FMN do, whereas PMS does not, enhance the Mehler reaction(Table 1).(2) A parallelism is found between the rates of ATP and CH_3CHO formation in the presence of Vit. K or FMN and also in the absence of added cofactor. The P/O value thus obtained is twice that of simultaneously determined K_3Fe(CN)_6 system and amounts to 2 when the coupling is complete(Table 2, 3).(3) The effects of pH, NH_4~+ and aging of chloroplasts on the Vit. K and on K_3Fe(CN)_6 systems are similar with respect to electron transport and phosphorylation(Fig. 1).(4) Under aerobic condition the P/O value remains practically unchanged upon varying the concentration of the cofactor(Fig. 2).The results of the present investigation showed that the same processes are involved in the Mehler reaction and photophosphorylation mediated by Vit. K or FMN. That the Vit. K or FMN mediated photophosphorylation is actually non-cyclic (?)n nature is conclusively proved. These cofactors are in fact"Hill oxidants" reversibly oxidized by molecular oxygen. Their possible physiological role in the photosynthetic process was briefly discussed.

    我們同时測定了小麦离体叶綠体的Mehler反应与光合磷酸化作用,結果指出: 1.光合磷酸化輔助因素Vit.K及FMN促进Mehler反应,PMS无影响。2.离体叶綠体不加輔助因素或加入Vit.K或FMN的情况下,ATP与CH_3CHO的形成量有一定的数量关系。由此算出的P/O值約为同时測定的K_3Fe(CN)_6系統的P/O值的二倍。在偶联得完全的制剂中,P/O值达2。3.无論对电子传递还是磷酸化作用,pH、解联剂(NH_4~+)及叶綠体保存时間对Vit.K及K_3Fe(CN)_6系統的影响均有一致的趋势。4.在有氧条件下,P/O值与輔助因素的浓度无关。本文結果指出了Mehler反应与FMN或Vit.K导致的光合磷酸化实际上发生在同一个过程中,这样就进一步肯定了Vit.K与FMN导致的光合磷酸化都是属于非循环方式。对它們在生理上的可能作用也作了簡短的討論。

    Three different homogentisate oxidases have been isolated from the liver extracts of rate by starch electrophoresis. Similar results were obtained with relatively purified enzymes prepared according to a modification of the method described by Tokuyama.The electrophoretic mobility of homogentisate oxidase I surpasses that of serum albumin. The Michaelis constant of this enzyme has the value of 1.4×10~(-3)M for homogentisic acid. In the absence of ferrous-ion this enzyme preparation completely losses its activity....

    Three different homogentisate oxidases have been isolated from the liver extracts of rate by starch electrophoresis. Similar results were obtained with relatively purified enzymes prepared according to a modification of the method described by Tokuyama.The electrophoretic mobility of homogentisate oxidase I surpasses that of serum albumin. The Michaelis constant of this enzyme has the value of 1.4×10~(-3)M for homogentisic acid. In the absence of ferrous-ion this enzyme preparation completely losses its activity. It may thus be suggested that Fe~(++) is a necessary cofactor for this enzyme. Oxidation of homogentisic acid by this enzyme is also inhibited by high concentrations of the substrate (6.6×10~(-3)M).Of the other two oxidases, II shifts only slightly towards the anode, while III migrates with a mobility almost equal to that of I, but towards the cathode. Their Michaelis constants both have the value of 5.0×10~(-4)M, being extremely close to the value of 4.0×10~(-4)M obtained by Tokuyama for beef liver homogentisate oxidase.Apart from significant differences in the values obtained for their K_m values, oxidase III differs from I also in an other respect-lack of requirement for Fe~(++) for enzymatic activity.The nature of the reactions which occur in the assay system in the absence of enzyme is also discussed.

    大白鼠肝脏尿黑酸氧化酶经丙酮,硫酸铵等步骤处理,再经过平板淀粉电泳的分离,此活力可以提高1000倍。利用平板淀粉电泳技术,由大白鼠肝脏中分离出三个具有尿黑酸氧化酶活力的酶蛋白。其中一个在电泳过程中移向正极,该部分酶制剂对反应系统中亚铁离子的要求比较严格,对尿黑酸的米氏常数等于1.4×10~(-3)M,高浓度底物有明显的抑制作用。第二个尿黑酸氧化酶亦趋向正极,但速度较慢,活力较低。第三个尿黑酸氧化酶的电泳位置接近负极端点,即使在没有任何辅因子参加的系统中亦具有一定水平的酶活力。后两个尿黑酸氧化酶对底物的米氏常数均为5.0×10~(-4)M,高浓度底物没有抑制现象发生。证明三个尿黑酸氧化酶催化同一个反应,而彼此的性质有区别,是一个具有多种分子形式的同工酶。本文对尿黑酸的自氧化以及影响自氧化的各种因子进行了分析讨论。

    Under weak illumination the photophosphorylation activity of sweet potato leaf cell preparation (LOP) was shown to be less influenced by the "light intensity effect" (Fig.1). It serves as an excellent experimental material in investigating the efficiency of photophosphorylation.The action spectra of both cyclic (endogenous cofactor and PMS) and non-cyclic (K_3Fe(CN)_6 and NADP) photophosphorylation catalyzed by sweet potato LCP within the orange-red region of the visible light were determined. In all the...

    Under weak illumination the photophosphorylation activity of sweet potato leaf cell preparation (LOP) was shown to be less influenced by the "light intensity effect" (Fig.1). It serves as an excellent experimental material in investigating the efficiency of photophosphorylation.The action spectra of both cyclic (endogenous cofactor and PMS) and non-cyclic (K_3Fe(CN)_6 and NADP) photophosphorylation catalyzed by sweet potato LCP within the orange-red region of the visible light were determined. In all the systems studied,relatively uniform efficiencies were observed between 620-680 mμ (Table 1 and Fig. 2).But as the wave length of the activating light exceeded 680mμ, the relative efficiencies of the non-cyclic photophosphorylation systems decreased abruptly, exhibiting a "red drop" phenomenon similar to those observed in photosynthesis and Hill reaction (Fig.2). In the case of cyclic photophosphorylation, relative efficiency at 700mμ light was nearly the same or a little higher than those under shorter wave lengths. When the wave length of the activating light went farther into the far-red region, the relative efficiency of cyclic photophosphorylation also dropped rapidly (Fig. 2).Both cyclic and non-cyclic photophosphorylation showed two-lights(640 and 708mμ)enhancement effect under aerobic conditions (Table 2). In H_2 atmosphere the efficiency of cyclic photophosphorylation at 708mμ light was raised and the two-lights enhancement effect disappeared (Table 4). It was concluded that the appearance of the "red drop" phenomenon and the two-lights enhancement effect in the cyclic photophosphorylating systems under aerobic conditions was due to the autooxidation of a part of the reduced cyclic cofactors. A second light reaction activated by shorter wave length light thus became necessary in order to reduce the oxidized intermediate and to enable the electron transport chain in full operation.The results of the present investigation substantiates the contention that two separate photochemical reactions, activated by different pigment systems, are present in the photosynthetic machinery of the chloroplast.

    我们利用光强效应较不显著的甘薯叶细胞制剂为材料,测定了循环(内源、PMS)及非循环光合磷酸化[K_3Fe(CN)_6,NADP~+]在橙—红光区段的作用光谱及双光增益效应,所得的主要结果如下: (1)在620—680mμ范围内,各系统作用光谱的起伏均很小,没有出现显著的高峰或低谷。(2)当波长超过680mμ时,非循环光合磷酸化系统的效率大降,出现与光合作用及希尔反应中相似的“红降”现象。以640mμ光与708mμ光同时照射,见到双光增益效应的存在。(3)循环光合磷酸化在700mμ光照下,相对效率与短波长光下相近或稍高。波长再向上移则效率也迅速降低。在有氧条件下,708mμ光下的效率也受同时加照的640mμ光的增益,但在无氧条件下则不显示增益效应。作者认为由于部分循环光合磷酸化辅助因子的自动氧化,远红光下放氧反应受阻,影响了光氧化物的还原及重复使用,因而使相对效率降低。本文结果从光合磷酸化的角度,支持叶绿体中存在着两个独立的、有不同色素参与的光化学反应。

     
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