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functional gene
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  功能基因
     The article expounds the structure of the human Y chromosome and it's main functional gene that correlates with spermatogenesis,chiefly discuss the deletion of each gene in AZF region such as USP9Y,RBM,DAZ,CDY with spermatogenic failure.
     现就人类Y染色体的结构及其与精子生成相关的主要功能基因的研究进展作一评述,重点对AZF区的USP9Y、RBM、DAZ、CDY等基因的缺失与精子生成障碍的关系进行了探讨。
短句来源
     Polymerase chain reaction-restricition fragment length polymorphism (RFLPs) was used to analyze the allele frequencies and genetype distribution of DRD2 gene TaqIA polymorphism, DRD3 functional gene Ser9Gly polymorphism, 5-HT2A gene A-1438G and T102C polymorphism.
     应用聚合酶链反应-限制性片段长度多态性法分析两组多巴胺D2受体基因TaqIA多态性、多巴胺D3受体功能基因Ser9Gly多态性、5-羟色胺2A受体基因A-1438G、T102C多态性位点的等位基因频率和基因型分布。
短句来源
     Functional gene fragments were chosen in wild and cultivated soybean with geographical representative for the study of the genetic patterns and the rule of its change during domestication process on DNA sequence level to learn the molecular process of soybean domestication and the molecular mechanism of soybean origin and evolution.
     本文选择有代表性的野生大豆和栽培大豆为材料,以大豆功能基因片段为研究对象,在DNA序列水平上研究野生大豆和栽培大豆的群体遗传结构及其在栽培驯化过程中的演变规律。 旨在进一步了解大豆栽培驯化的分子过程,为揭示大豆起源与进化的分子机理提供必要的研究基础。
短句来源
     Screening the functional gene cDc42 - like protein from adult clonorchis sinensis with bio - information methods.
     应用生物信息学方法筛选华支睾吸虫功能基因-Cdc42样蛋白
短句来源
     2.Through determining the sequences of special fragment and analyzing sequence homology of reported chiB gene, the results showed, the cloned fragment is a functional gene of about 1500bp, its homology was up to 99% with the chiB gene sequence of Serratia marcescens X15208 and AB015997, 87% with Serratia liquefaciens AF399871, but it had lower homology with the chiB gene of other different bacteria. It elementarily indicated that the fragment was chitinase B gene of Serratia marcescens;
     2. 通过特异片段的测序和与报道的 chiB 基因的序列同源性分析,结果显示,该克隆片段是阅读框为 1500bp 的功能基因,与 Serratia marcescens X15208、AB015997 的 chiB 基因序列的同源性均达到了 99%,与 Serratia liquefaciens AF399871chiB 基因的同源性达 87 %,而与其它不同菌种的 chiB 基因同源性较低,初步表明该片段是粘质沙雷氏菌几丁质酶 chiB 基因;
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  功能性基因
     The functional gene fragments producing CTX-M-14, 1.8 kb, 2.4 kb and 3.1 kb in size, were cloned by shotgun cloning experiment. DNA sequencing and it was shown that CTX-M-14 gene was located on transferable gene elements transposon of the plasmid.
     采用鸟枪法克隆到 1.8kb、2 .4kb、3.1kb产CTX M 14的功能性基因片段 ,序列分析显示其定位在耐药质粒上转座子基因结构中 ;
短句来源
     Experimental study on herpes simplex virus 1 functional gene latency in cornea
     单纯疱疹病毒1型功能性基因在角膜内潜伏感染的实验研究
短句来源
     Objective To investigate the structural characteristic of functional gene fragment coding CTX-M-14-type extended-spectrum beta-lactamases (ESBLs).
     目的 研究产CTX M 14型超广谱 β 内酰胺酶 (ESBLs)功能性基因片段的结构特征。
短句来源
     This study provides the scientific proof to the feasibility of functional gene therapy of complications such as ischemia-reperfusion injury, rejection and infection after rat lung transplantation.
     本实验为进一步开展大鼠肺移植术后缺血 -再灌注损伤、免疫排斥反应和感染的功能性基因治疗实验研究的可行性提供了依据
短句来源
     The results lay the fundations of functional gene transfer to lung cancer cells and further to the gene therapy of lung cancer.
     本实验为阳离子脂质体介导肺癌细胞功能性基因的转移及进一步的肺癌基因治疗打下了基础。
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  “functional gene”译为未确定词的双语例句
     And human and mouse ALR cDNA have been cloned thereafter. Meanwhile scERV1 cDNA has been cloned from yeast and found ERV1 is a functional gene related to mitochondrial function and phosphorylation.
     与此同时,从酵母细胞中克隆了与线粒体氧化———磷酸化功能密切相关的ERV1基因,然后克隆了人的ERV1同源基因,从功能上证实人ERV1基因可以替代酵母细胞ERV1的功能,拯救酵母细胞ERV1基因突变。
短句来源
     Conclusion:It was suggested that obtained VH gene was potentially functional gene of the McAb COC183B2 against human ovarian carcinoma
     结论:该VH基因为抗人卵巢癌单抗COC183B2功能性重链可变区基因。
短句来源
     Meanwhile Bioinformatics has played an important role in the study of functional gene identification, illness diagnosis, gene expression profiling and protein function.
     同时,随着人类基因组计划进一步的快速发展,生物信息学在人类疾病与功能基因的发现与识别、基因与蛋白质的表达与功能研究方面都发挥着关键的作用。
短句来源
     Thereinto, the MT-lg and MT-1K are reported at the first time as a functional gene.
     在本文MT-1g和MT-1K第一次被鉴定为有功能的基因。
短句来源
     Detection at molecular level and anti-disease identification of the T1, T2 and T3 transgenic wheats with Bcl and Rip genes indicated that segregation ratios of nptⅡ selective gene and functional gene Rip were consistent with Mendel’s law,but functional gene Bcl was not in T1 generation.
     对转Bcl、Rip基因小麦的T1、T2和T3代植株进行了分子检测和抗病性鉴定,并根据检测结果对其遗传行为做了探讨与分析。 T1代检测结果表明,选择标记基因nptⅡ的分离符合孟德尔遗传规律;
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  functional gene
With the example of yeast genes, context organization was compared for functional gene regions (promoter, 5"-UTR, 3"-UTR) and tested for association with the level of gene expression.
      
Alternative ways take advantage of new extrachromosomal vector systems (pETE, pETR) and the functional gene inactivation test.
      
A full-size functional gene encoding the human ribosomal protein S21 was cloned and characterized.
      
The fertilized sea urchin eggs and dissociated embryonic cells at the blastula stage were treated with plasmids containing both the functional gene gal4 and the gene devoid of the regions encoding the activator domain.
      
The transfection of embryonic sea urchin eggs with the functional gene led to cell dedifferentiation and formation of tumor-like structures in the embryos or increased number of embryonic cells in culture.
      
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A cDNA library of rice (Oryza sativa ssp. indica cv. “Guanglu' ai 4”) etiolated shoot was constructed using Lambda ZAPⅡ vector. After analysing the partial sequences of 100 randomly selected clones and database comparisons to rice and other plants, 13% cDNA clones could be identified and 12% cDNAs had high degree of sequence similarity to partial sequence from rice or other species, whose function is still unknown. The remaining 75% cDNAs showed little or no similarity to genes in the database and might...

A cDNA library of rice (Oryza sativa ssp. indica cv. “Guanglu' ai 4”) etiolated shoot was constructed using Lambda ZAPⅡ vector. After analysing the partial sequences of 100 randomly selected clones and database comparisons to rice and other plants, 13% cDNA clones could be identified and 12% cDNAs had high degree of sequence similarity to partial sequence from rice or other species, whose function is still unknown. The remaining 75% cDNAs showed little or no similarity to genes in the database and might represent novel genes. It demonstrates the suitability of this library for large scale sequencing from which more information of functional genes will result.

以水稻 (Oryza sativa)“广陆矮 4号”黄化苗为材料 ,用 λZAP 为载体构建了 c DNA文库。对随机挑取的 1 0 0个 c DNA进行部分顺序测定后 ,与水稻和其它植物已知的基因结构作同源性比较分析 ,表明1 3%的 c DNA克隆可以确定功能 ,1 2 %的 c DNA克隆与其它植物中未知功能的基因片段有同源性 ,其余75%的克隆则表现出极少相似性或无任何同源性 ,从中可能发现新的基因。结果表明这个 c DNA文库适宜于进行大量顺序分析。由此将会获得更多功能性基因的信息。

In this report,an enkaryotic cell expression vector which contains the cDNA encoding an inducible nitric oxide synthase(iNOS) from RAW264.7 macrophages was designed and constructed using the molecular biologic procedures.The plasmid pBluscript Ⅱ KS(+)iNOS was digested with double enzymes,Hinc Ⅱ and Not Ⅰ. A 3973bp DNAfragment carrying the iNOS gene was purified from agarose electrophoresis gels and ligated to the polylinker sites betweem Hind Ⅲ and Not Ⅰ in the pRc/CMV,an enkaryotic expression vector in directional...

In this report,an enkaryotic cell expression vector which contains the cDNA encoding an inducible nitric oxide synthase(iNOS) from RAW264.7 macrophages was designed and constructed using the molecular biologic procedures.The plasmid pBluscript Ⅱ KS(+)iNOS was digested with double enzymes,Hinc Ⅱ and Not Ⅰ. A 3973bp DNAfragment carrying the iNOS gene was purified from agarose electrophoresis gels and ligated to the polylinker sites betweem Hind Ⅲ and Not Ⅰ in the pRc/CMV,an enkaryotic expression vector in directional cloning.2 out of 14 transformants,clone2 # and clone8 #,were identified with the cleavage of EcoR Ⅰ and Sal Ⅰ to be a recombinant plasmid pCMV iNOS.The restriction analysis of pCMV iNOS with Not Ⅰ or Kpn Ⅰ+Sal Ⅰ revealed that the position and size of cDNA insertion were consistent with the sequence of prediction.Furthermore,the plasmid pCMV iNOS was analyzed by restriction endonuleases such as EcoR Ⅴ+Xba Ⅰ,EcoRⅤ+Sca Ⅰ,BamH Ⅰ+Xba Ⅰor BamH Ⅰ+Bgl Ⅱ.It was verified that iNOS gene was inserted into pRc/CMV in sense orientation.PCR analysis was carried on using the pCMV iNOS plasmid DNA as a template and the specific primers corresponding to cDNA sequence of iNOS,The result demonstrated that amplification product was a 451bp fragment,thus indicating the presence of iNOS coding sequence in the recombinant vector.The characterization of the expression plasmid displays that the plasmid pCMV iNOS carries the cDNA seqence that may encode the overall 3432 nucleotide open reading frame of iNOS,but delete the partial 5' un translated regions,which should be useful for the functional gene expression at high levels.lts structure also includes a cytomegalovirus promoter,a simian virus 40 polyadenylation site and a neomycin phosphotransferase(neo)selectable marker so that it may be generally available for the use to transfect into a variety of mammaliam cells.Construction of plasmid pCMV iNOS will be extremely valuable,and provide a novel thought and strategy to study the gene regulation of enkaryotic expression of iNOS and clarify the molecular mechanisms by which NO/iNOS may regulate the biological functions in nervous,cardiovascular and immune system.

采用分子克隆技术,设计并构建来源RAW264.7巨噬细胞iNOS基因cDNA的真核表达载体。以HincⅡ+NotⅠ双酶解pKSiNOS,电泳回收3973bp编码iNOS的cDNA序列,克隆入真核表达载体pRc/CMV的HindⅢ和NotⅠ位点。用EcoRⅠ和SalⅠ酶切鉴定,初步筛选2#和8#克隆为重组子pCMViNOS。经NotⅠ和KpnI+Sa1Ⅰ酶切结果表明,重组克隆外源iNOS基因插入位点和片段大小正确。以EcoRV+XbaⅠ、EcoRV+ScaⅠ、BamHⅠ+XbaⅠ或BamHⅠ+BglⅡ双酶切图谱分析,均证明iNOS基因正向插入真核表达载体。用iNOS基因特异性引物,PCR分析质粒pCMViNOS,扩增出451bp片段,提示重组质粒含有iNOS基因序列。pCMViNOS特点是携有iNOS的cDNA序列,包括编码iNOS开放阅读框架,但删除5'端部分非编码区,有利于高效表达。还含有巨细胞病毒强启动子、ploy(A)加接信号和neo标志基因,具有转染多种哺乳类细胞通用性。因此,重组表达质粒pCMViNOS的构建可望为研究iNOS基因真核表达调控,阐明NO/iNOS调节神经、心血管和免疫系统的分子机理?

Objective: To amplify and sequence the heavy chain variable region (VH ) gene of monoclonal antibody (McAb) COC183B2 against human ovarian carcinoma. Methods: The VH gene of mouse McAb COC183B2 against human ovarian carcinoma was amplified by RTPCR . The PCR product was then cloned into PUC19 vector. The recombinants were sequenced by Sanger's method. The VH gene was compared with Gene Bank and published mouse VH genes. Results: It was proved that a fulllength VH gene was 354 bp, the VH gene was...

Objective: To amplify and sequence the heavy chain variable region (VH ) gene of monoclonal antibody (McAb) COC183B2 against human ovarian carcinoma. Methods: The VH gene of mouse McAb COC183B2 against human ovarian carcinoma was amplified by RTPCR . The PCR product was then cloned into PUC19 vector. The recombinants were sequenced by Sanger's method. The VH gene was compared with Gene Bank and published mouse VH genes. Results: It was proved that a fulllength VH gene was 354 bp, the VH gene was a member of mouse Ig heavy chain subgroup miscellaneous and originated from rearrangement of VH、 Dsp2.5 and MUSJH4 gene. The VH gene sequence was registered by Gene Bank(accession No. AF045023). Conclusion:It was suggested that obtained VH gene was potentially functional gene of the McAb COC183B2 against human ovarian carcinoma

目的:分离抗人卵巢癌单克隆抗体COC183B2重链可变区(VH)基因并测定其序列。方法:用反转录PCR扩增抗人卵巢癌单抗COC183B2VH基因,将其克隆入PUC19载体,重组子用Sanger’s双脱氧链终止法测定序列,将序列与GeneBank及已发表的抗体序列比较。结果:VH基因全长354bp,属鼠免疫球蛋白重链混杂亚类(subgroupmiscelaneous),由种系基因的VH,Dsp2.5及MUSJH4重排而来。该VH基因序列已被GeneBank收录(accessionNoAF045023)。结论:该VH基因为抗人卵巢癌单抗COC183B2功能性重链可变区基因。

 
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