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functional gene
相关语句
  功能基因
    Construction and Functional Gene Screening of cDNA Library of Dunaliella salina
    盐生杜氏藻(Dunaliella salina)cDNA文库构建及功能基因筛选(英文)
短句来源
    Comparative Analysis of Internal Repeating Segments in Proteins of Source Functional Gene with Derivate Retropseudogene
    反转录假基因和其对应的源功能基因的蛋白质内部重复片段的比较
短句来源
    2.Through determining the sequences of special fragment and analyzing sequence homology of reported chiB gene, the results showed, the cloned fragment is a functional gene of about 1500bp, its homology was up to 99% with the chiB gene sequence of Serratia marcescens X15208 and AB015997, 87% with Serratia liquefaciens AF399871, but it had lower homology with the chiB gene of other different bacteria. It elementarily indicated that the fragment was chitinase B gene of Serratia marcescens;
    2. 通过特异片段的测序和与报道的 chiB 基因的序列同源性分析,结果显示,该克隆片段是阅读框为 1500bp 的功能基因,与 Serratia marcescens X15208、AB015997 的 chiB 基因序列的同源性均达到了 99%,与 Serratia liquefaciens AF399871chiB 基因的同源性达 87 %,而与其它不同菌种的 chiB 基因同源性较低,初步表明该片段是粘质沙雷氏菌几丁质酶 chiB 基因;
短句来源
    Two kinds of IgM cDNA was cloned from bovine spleen total RNA. Sequencing the cDNAclones show that the last exon of JH gene (accession No.AY149283) JH6 is a functional gene encoding part ofCDR3 and whole FR4 region of bovine IgM.
    提取牛脾脏总RNA,RT-PCR扩增IgMcDNA,测序结果显示:序列号为AY149283的JH基因中第六外显子JH6是一个功能基因,它可以编码牛IgM的部分CDR3区和完整的FR4区,从2种IgMcDNA克隆的测序结果可以看出,其恒定区序列分别与序列号为U63637和AY230207的2种抗体重链恒定区μ基因(Cμ)cDNA序列相同.
短句来源
    Soil microbial ecological process and microbial functional gene diversity.
    土壤微生物生态过程与微生物功能基因多样性
短句来源
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  “functional gene”译为未确定词的双语例句
    Thereinto, the MT-lg and MT-1K are reported at the first time as a functional gene.
    在本文MT-1g和MT-1K第一次被鉴定为有功能的基因。
短句来源
    The PCR product was cut by two restriction enzymes, purified and cloned into the vector pGEX-4T-1. The engineering strains were incubated at 37 until OD600 ,0.6-0.8, then IPTG was added to reach the concentration of 0.75mmol/L to induce the fusion expression of the functional gene at 37 for 7 h.
    将该序列连接到pGEX-4T-1表达载体上构成重组质粒pGEX-4T-1-MP,将该质粒转化到E. coli BL21 (DE3) 37℃在LB培养基中培养,至OD600=0.6-0.8,加入浓度为0.75mM的IPTG诱导表达。
短句来源
    Conclusion:It was suggested that obtained VH gene was potentially functional gene of the McAb COC183B2 against human ovarian carcinoma
    结论:该VH基因为抗人卵巢癌单抗COC183B2功能性重链可变区基因。
短句来源
    [Conclusion] The obtained V L gene was a potentially functional gene of anti idiotypic monoclonal antibody NP30 of Schistosoma japonicum .
    [结论 ]该VL 基因为日本血吸虫单克隆抗独特型抗体NP30轻链可变区基因。
短句来源
    And human and mouse ALR cDNA have been cloned thereafter. Meanwhile scERV1 cDNA has been cloned from yeast and found ERV1 is a functional gene related to mitochondrial function and phosphorylation.
    与此同时,从酵母细胞中克隆了与线粒体氧化———磷酸化功能密切相关的ERV1基因,然后克隆了人的ERV1同源基因,从功能上证实人ERV1基因可以替代酵母细胞ERV1的功能,拯救酵母细胞ERV1基因突变。
短句来源
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  functional gene
With the example of yeast genes, context organization was compared for functional gene regions (promoter, 5"-UTR, 3"-UTR) and tested for association with the level of gene expression.
      
Alternative ways take advantage of new extrachromosomal vector systems (pETE, pETR) and the functional gene inactivation test.
      
A full-size functional gene encoding the human ribosomal protein S21 was cloned and characterized.
      
The fertilized sea urchin eggs and dissociated embryonic cells at the blastula stage were treated with plasmids containing both the functional gene gal4 and the gene devoid of the regions encoding the activator domain.
      
The transfection of embryonic sea urchin eggs with the functional gene led to cell dedifferentiation and formation of tumor-like structures in the embryos or increased number of embryonic cells in culture.
      
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A cDNA library of rice (Oryza sativa ssp. indica cv. “Guanglu' ai 4”) etiolated shoot was constructed using Lambda ZAPⅡ vector. After analysing the partial sequences of 100 randomly selected clones and database comparisons to rice and other plants, 13% cDNA clones could be identified and 12% cDNAs had high degree of sequence similarity to partial sequence from rice or other species, whose function is still unknown. The remaining 75% cDNAs showed little or no similarity to genes in the database and might...

A cDNA library of rice (Oryza sativa ssp. indica cv. “Guanglu' ai 4”) etiolated shoot was constructed using Lambda ZAPⅡ vector. After analysing the partial sequences of 100 randomly selected clones and database comparisons to rice and other plants, 13% cDNA clones could be identified and 12% cDNAs had high degree of sequence similarity to partial sequence from rice or other species, whose function is still unknown. The remaining 75% cDNAs showed little or no similarity to genes in the database and might represent novel genes. It demonstrates the suitability of this library for large scale sequencing from which more information of functional genes will result.

以水稻 (Oryza sativa)“广陆矮 4号”黄化苗为材料 ,用 λZAP 为载体构建了 c DNA文库。对随机挑取的 1 0 0个 c DNA进行部分顺序测定后 ,与水稻和其它植物已知的基因结构作同源性比较分析 ,表明1 3%的 c DNA克隆可以确定功能 ,1 2 %的 c DNA克隆与其它植物中未知功能的基因片段有同源性 ,其余75%的克隆则表现出极少相似性或无任何同源性 ,从中可能发现新的基因。结果表明这个 c DNA文库适宜于进行大量顺序分析。由此将会获得更多功能性基因的信息。

In this report,an enkaryotic cell expression vector which contains the cDNA encoding an inducible nitric oxide synthase(iNOS) from RAW264.7 macrophages was designed and constructed using the molecular biologic procedures.The plasmid pBluscript Ⅱ KS(+)iNOS was digested with double enzymes,Hinc Ⅱ and Not Ⅰ. A 3973bp DNAfragment carrying the iNOS gene was purified from agarose electrophoresis gels and ligated to the polylinker sites betweem Hind Ⅲ and Not Ⅰ in the pRc/CMV,an enkaryotic expression vector in directional...

In this report,an enkaryotic cell expression vector which contains the cDNA encoding an inducible nitric oxide synthase(iNOS) from RAW264.7 macrophages was designed and constructed using the molecular biologic procedures.The plasmid pBluscript Ⅱ KS(+)iNOS was digested with double enzymes,Hinc Ⅱ and Not Ⅰ. A 3973bp DNAfragment carrying the iNOS gene was purified from agarose electrophoresis gels and ligated to the polylinker sites betweem Hind Ⅲ and Not Ⅰ in the pRc/CMV,an enkaryotic expression vector in directional cloning.2 out of 14 transformants,clone2 # and clone8 #,were identified with the cleavage of EcoR Ⅰ and Sal Ⅰ to be a recombinant plasmid pCMV iNOS.The restriction analysis of pCMV iNOS with Not Ⅰ or Kpn Ⅰ+Sal Ⅰ revealed that the position and size of cDNA insertion were consistent with the sequence of prediction.Furthermore,the plasmid pCMV iNOS was analyzed by restriction endonuleases such as EcoR Ⅴ+Xba Ⅰ,EcoRⅤ+Sca Ⅰ,BamH Ⅰ+Xba Ⅰor BamH Ⅰ+Bgl Ⅱ.It was verified that iNOS gene was inserted into pRc/CMV in sense orientation.PCR analysis was carried on using the pCMV iNOS plasmid DNA as a template and the specific primers corresponding to cDNA sequence of iNOS,The result demonstrated that amplification product was a 451bp fragment,thus indicating the presence of iNOS coding sequence in the recombinant vector.The characterization of the expression plasmid displays that the plasmid pCMV iNOS carries the cDNA seqence that may encode the overall 3432 nucleotide open reading frame of iNOS,but delete the partial 5' un translated regions,which should be useful for the functional gene expression at high levels.lts structure also includes a cytomegalovirus promoter,a simian virus 40 polyadenylation site and a neomycin phosphotransferase(neo)selectable marker so that it may be generally available for the use to transfect into a variety of mammaliam cells.Construction of plasmid pCMV iNOS will be extremely valuable,and provide a novel thought and strategy to study the gene regulation of enkaryotic expression of iNOS and clarify the molecular mechanisms by which NO/iNOS may regulate the biological functions in nervous,cardiovascular and immune system.

采用分子克隆技术,设计并构建来源RAW264.7巨噬细胞iNOS基因cDNA的真核表达载体。以HincⅡ+NotⅠ双酶解pKSiNOS,电泳回收3973bp编码iNOS的cDNA序列,克隆入真核表达载体pRc/CMV的HindⅢ和NotⅠ位点。用EcoRⅠ和SalⅠ酶切鉴定,初步筛选2#和8#克隆为重组子pCMViNOS。经NotⅠ和KpnI+Sa1Ⅰ酶切结果表明,重组克隆外源iNOS基因插入位点和片段大小正确。以EcoRV+XbaⅠ、EcoRV+ScaⅠ、BamHⅠ+XbaⅠ或BamHⅠ+BglⅡ双酶切图谱分析,均证明iNOS基因正向插入真核表达载体。用iNOS基因特异性引物,PCR分析质粒pCMViNOS,扩增出451bp片段,提示重组质粒含有iNOS基因序列。pCMViNOS特点是携有iNOS的cDNA序列,包括编码iNOS开放阅读框架,但删除5'端部分非编码区,有利于高效表达。还含有巨细胞病毒强启动子、ploy(A)加接信号和neo标志基因,具有转染多种哺乳类细胞通用性。因此,重组表达质粒pCMViNOS的构建可望为研究iNOS基因真核表达调控,阐明NO/iNOS调节神经、心血管和免疫系统的分子机理?

Objective: To amplify and sequence the heavy chain variable region (VH ) gene of monoclonal antibody (McAb) COC183B2 against human ovarian carcinoma. Methods: The VH gene of mouse McAb COC183B2 against human ovarian carcinoma was amplified by RTPCR . The PCR product was then cloned into PUC19 vector. The recombinants were sequenced by Sanger's method. The VH gene was compared with Gene Bank and published mouse VH genes. Results: It was proved that a fulllength VH gene was 354 bp, the VH gene was...

Objective: To amplify and sequence the heavy chain variable region (VH ) gene of monoclonal antibody (McAb) COC183B2 against human ovarian carcinoma. Methods: The VH gene of mouse McAb COC183B2 against human ovarian carcinoma was amplified by RTPCR . The PCR product was then cloned into PUC19 vector. The recombinants were sequenced by Sanger's method. The VH gene was compared with Gene Bank and published mouse VH genes. Results: It was proved that a fulllength VH gene was 354 bp, the VH gene was a member of mouse Ig heavy chain subgroup miscellaneous and originated from rearrangement of VH、 Dsp2.5 and MUSJH4 gene. The VH gene sequence was registered by Gene Bank(accession No. AF045023). Conclusion:It was suggested that obtained VH gene was potentially functional gene of the McAb COC183B2 against human ovarian carcinoma

目的:分离抗人卵巢癌单克隆抗体COC183B2重链可变区(VH)基因并测定其序列。方法:用反转录PCR扩增抗人卵巢癌单抗COC183B2VH基因,将其克隆入PUC19载体,重组子用Sanger’s双脱氧链终止法测定序列,将序列与GeneBank及已发表的抗体序列比较。结果:VH基因全长354bp,属鼠免疫球蛋白重链混杂亚类(subgroupmiscelaneous),由种系基因的VH,Dsp2.5及MUSJH4重排而来。该VH基因序列已被GeneBank收录(accessionNoAF045023)。结论:该VH基因为抗人卵巢癌单抗COC183B2功能性重链可变区基因。

 
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