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functional gene
相关语句
  功能基因
    In recent years, with the development of molecular genetics and molecular biology, more and more approaches were currently applied to the study of fungal functional gene during fungi interaction with host.
    近年来随着分子生物学和基因组学的迅猛发展,推动了诸如条锈菌等以前难以深入研究的病原菌及其与寄主互作过程中功能基因的分析和鉴定。
短句来源
  “functional gene”译为未确定词的双语例句
    The functional gene of Activator Protein was cloned from M. griesea and a strain was acquired, in which the soluble Activator Protein with bioactivity was expressed stably. This research laid the foundation for further study of the structure and property of Activator Protein.
    克隆了稻瘟菌激活蛋白基因,首次获得了稳定表达可溶性稻瘟菌激活蛋白的菌株,并确定了表达蛋白的活性,该研究为确定激活蛋白的结构与功能及理化性质奠定基础。
短句来源
    The functional gene of Activator Protein was cloned and expressed in E.
    从稻瘟菌中克隆了激活蛋白基因并在E.
短句来源
    A mutagenesis technique system of B.subtilis NCD-2 was generated by transposon Tn917 mutageneisis,and the functional gene encoding the antipeptide was knocked off by the transponson. In this study,B.subtilis NCD-2 was transformed with a plasmid pTV1 carrying transponson Tn917 by protoplast methods. Twenty tranformed NCD-2 strains were screened resistant against chloromycetin,erythromycin and lincomycin.
    本研究着重通过原生质体法与诱导转座方法,建立了携带转座子Tn917质粒pTV1对枯草芽孢杆菌NCD-2野生菌株的转化体系与转座子突变技术,获得1500多个转座子插入突变子。
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  functional gene
With the example of yeast genes, context organization was compared for functional gene regions (promoter, 5"-UTR, 3"-UTR) and tested for association with the level of gene expression.
      
Alternative ways take advantage of new extrachromosomal vector systems (pETE, pETR) and the functional gene inactivation test.
      
A full-size functional gene encoding the human ribosomal protein S21 was cloned and characterized.
      
The fertilized sea urchin eggs and dissociated embryonic cells at the blastula stage were treated with plasmids containing both the functional gene gal4 and the gene devoid of the regions encoding the activator domain.
      
The transfection of embryonic sea urchin eggs with the functional gene led to cell dedifferentiation and formation of tumor-like structures in the embryos or increased number of embryonic cells in culture.
      
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Avirulence genes are functional genes in pathogens that determine expression of the race-specific resistance in hosts. By using the technique of random amplified polymorphic DNAs(RAPD), this study identified a new DNA marker OPEl21400 that was closely linked to the avirulence gene, AVR-Pikm, in rice blast pathogen Magnaporthe grisea. Based on the nucleotide sequence of OPE121400 a pair of 17 nt primers was designed and used for PCR detection of DNA polymorphism in a mapping population, which...

Avirulence genes are functional genes in pathogens that determine expression of the race-specific resistance in hosts. By using the technique of random amplified polymorphic DNAs(RAPD), this study identified a new DNA marker OPEl21400 that was closely linked to the avirulence gene, AVR-Pikm, in rice blast pathogen Magnaporthe grisea. Based on the nucleotide sequence of OPE121400 a pair of 17 nt primers was designed and used for PCR detection of DNA polymorphism in a mapping population, which was generated by crossing an avirulent strain S1522 with a virulent strain S159. A single DNA band with similar length to the RAPD marker OPE121400 was generated by the PCR in all the avirulent progenies but not in the virulent ones except for two recombinants. The genetic distance from the marker to AVR-Pikm was calculated as 1. 89 cM, which was 2. 86 cM closer to the gene than OPO121000, a RAPD marker previously identified by our group. Although lied in the same side with OPO121000, OPE121400 will be useful for locating the position of AVR-Pikm in the chromosome and orientating contigs for further screening of linked SSR markers that lie in the opposite side.

无毒基因是病原物中决定寄主抗病性表达的功能基因,其功能的丧失导致毒性小种的产生。本研究利用随机引物扩增DNA多态性技术,从稻瘟病菌菌株S1522中新筛选到1个与无毒基因AVR-Pikm紧密连锁的DNA标记OPE121400。根据OPE121400的核苷酸序列,设计了1对含有17个核苷酸的特异性SCAR引物,并利用该引物对无毒表型亲本S1522和毒性表型亲本S159及其有性杂交后代的108个菌株进行了PCR扩增。结果表明:所有无毒表型的菌株均能特异性扩增出1条与OPE121400大小相近的DNA片段,而毒性表型的菌株除2个重组体外,均不能扩增出此片段。根据计算,这一SCAR标记与目标无毒基因AVR-Pikm之间的遗传距离为1.89 cM,与本研究小组先前报道的另一个标记OPO121000位于目标基因的同一侧,但与OPO121000相比,距目标基因近了2.86 cM。本标记的获得将有助于确定AVR-Pikm在染色体上的位置,有助于确定用于进一步筛选位于相反一侧的连锁标记的重叠群区域。

Rice blast disease, caused by heterothallic ascomycete Magnaporthe grisea, is one of the most serious (fungal) diseases of rice throughout the world. The disease attacks rice plants throughout the season and causes severe yield losses. The pathogenesis of M.grisea is due to a complex process that spans the entire life cycle of the (pathogen.) The process including germination of conidia, formation of appressoria, differentiation of penetration pegs and proliferation of infectious hyphae is controlled by many...

Rice blast disease, caused by heterothallic ascomycete Magnaporthe grisea, is one of the most serious (fungal) diseases of rice throughout the world. The disease attacks rice plants throughout the season and causes severe yield losses. The pathogenesis of M.grisea is due to a complex process that spans the entire life cycle of the (pathogen.) The process including germination of conidia, formation of appressoria, differentiation of penetration pegs and proliferation of infectious hyphae is controlled by many genes. The interaction between M.grisea and rice is based on the gene-for-gene hypothesis and the defense responses are often activated by the action of the pathogen avirulence (Avr) gene and the host resistance (R) gene. The studies on molecular biology and genetic mechanism of (pathogenicity) of M.grisea have occupied pathologists and mycologists for several decades. The present paper reviewed the (research) progress concerning molecular genetics of pathogenicity of the fungus and its genetic diversity and variation, and summarized research methods for the functional genes.

由稻瘟病菌引起的稻瘟病是水稻生产上危害最为严重的真菌病害,对世界粮食生产造成巨大损失。稻瘟病菌成功侵染寄主包括分生孢子萌发、附着胞形成、侵染钉分化和侵染性菌丝扩展等一系列错综复杂的过程,其中每一环节都是由特定基因控制的。稻瘟病菌与水稻的互作符合经典的基因对基因学说,二者的不亲和互作是无毒基因与抗病基因相互作用的结果。近几十年来,世界各国科学家对稻瘟病菌致病性的生物学及其遗传的分子机制进行了深入的研究。文章就稻瘟病菌致病性的分子遗传学及其遗传变异机制研究进行了综述,同时对功能基因的研究方法进行了总结。

Avirulence genes,which determine if the specific resistance is expressed in host cultivars,are functional genes in pathogens.Lose of the gene function usually results in generation of virulent races in the pathogen population.In previous studies,two SCAR Sequence-characterized amplified region makers SCO12_ 946 and SCE12_ 1406 linked with avirulence gene AVR-Pik~m in M.grisea were isolated.In this investigation,the two SCAR markers were mapped on the chromosome I.On the basis of this...

Avirulence genes,which determine if the specific resistance is expressed in host cultivars,are functional genes in pathogens.Lose of the gene function usually results in generation of virulent races in the pathogen population.In previous studies,two SCAR Sequence-characterized amplified region makers SCO12_ 946 and SCE12_ 1406 linked with avirulence gene AVR-Pik~m in M.grisea were isolated.In this investigation,the two SCAR markers were mapped on the chromosome I.On the basis of this result,four more DNA markers,SSR47T34,SSR50CA24,SSR52TAGG18 and SSR56A28 linked with AVR-Pik~m,were identified by utilizing the whole genome draft sequence of M.grisea 70-15 and SSR Simple sequence repeat technology.Further analysis indicated that the four SSR markers lay on the side opposite to the two SCAR markers;the genetic distance between the four SSR markers and AVR-Pik~m is 4.90,7.01,19.12,21.94 cM,respectively.This mapping will facilitate the isolation of AVR-Pik~m by chromosome walking.

无毒基因是病原物中决定寄主抗病性表达与否的功能基因,其功能的丧失导致毒性小种的产生。在先前的研究中,本研究小组从稻瘟病菌中分离了与无毒基因AVR-P ikm连锁的2个SCAR标记SCO12946和SCE121406。在本研究中,作者首先将这2个标记定位到稻瘟菌第1号染色体上;然后,利用稻瘟菌70-15全基因组草图序列和SSR技术,又分离了与无毒基因AVR-P ikm连锁的4个SSR标记:SSR47T34、SSR50CA24、SSR52TAGG18和SSR56A28。进一步分析表明:上述4个SSR标记位于与SCO12946和SCE121406相反的一侧,与AVR-P ikm位点的遗传距离分别为4.90、7.01、19.12和21.94 cM,无毒基因AVR-P ikm位于SCE121406和SSR47T34之间。本研究获得的稻瘟菌无毒基因AVR-P ikm的精细定位为通过染色体步移法克隆该基因奠定了基础。

 
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