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functional gene
相关语句
  功能基因
    Evaluation of Rice Germplasm Using SSR Markers of Functional Gene in Rice
    利用水稻功能基因SSR标记鉴定水稻种质资源
短句来源
    Progress in Functional Gene Cloning of Camellia sinensis
    茶树重要功能基因克隆研究进展
短句来源
    Functional gene fragments were chosen in wild and cultivated soybean with geographical representative for the study of the genetic patterns and the rule of its change during domestication process on DNA sequence level to learn the molecular process of soybean domestication and the molecular mechanism of soybean origin and evolution.
    本文选择有代表性的野生大豆和栽培大豆为材料,以大豆功能基因片段为研究对象,在DNA序列水平上研究野生大豆和栽培大豆的群体遗传结构及其在栽培驯化过程中的演变规律。 旨在进一步了解大豆栽培驯化的分子过程,为揭示大豆起源与进化的分子机理提供必要的研究基础。
短句来源
    Progress in the Functional Gene Research of Medicinal Plants
    药用植物功能基因
短句来源
    Detection at molecular level indicated that segregation ratios of selective gene npt II and functional gene Rip were consistent with Mendel' s law, but functional gene Bel was not in Ti generation.
    对转基因植株分析,发现转基因植株中外源基因在T_1多为单拷贝,在稳定遗传的T_2代株系中多为双拷贝。 根据分子检测检测,对转基因植株进行了遗传分析,发现在T_1代选择标记基因npt Ⅱ和功能基因Rip的分离符合孟德尔遗传规律;
短句来源
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  “functional gene”译为未确定词的双语例句
    Detection at molecular level and anti-disease identification of the T1, T2 and T3 transgenic wheats with Bcl and Rip genes indicated that segregation ratios of nptⅡ selective gene and functional gene Rip were consistent with Mendel’s law,but functional gene Bcl was not in T1 generation.
    对转Bcl、Rip基因小麦的T1、T2和T3代植株进行了分子检测和抗病性鉴定,并根据检测结果对其遗传行为做了探讨与分析。 T1代检测结果表明,选择标记基因nptⅡ的分离符合孟德尔遗传规律;
短句来源
    Screening the T-DNA-inserted Rice Mutant Pool and Cloning a Functional Gene, DH1, Which is Required for the Formation of the Floral Glumes in Rice
    水稻T-DNA插入突变体库的筛选及与水稻颖花颖壳发育相关基因的克隆与功能分析
短句来源
    Growth status and survival rate of yeast cells were improved by over-expressing TaNHX2 gene. All the above results indicated that TaNHX2 may be a functional gene in the stress tolerance of crops. .
    在酵母中过表达TaNHX2基因能有效改进酵母突变体在各种胁迫下的生长状况和存活率,表明TaNHX2有望作为一种新的基因资源用于改善作物耐逆性。
短句来源
    Furthermore, for a degenerated hull mutant, we cloned and validated its corresponding functional gene, DH1, and assayed its expression pattern.
    对获得的一个颖花颖壳缺失的突变体,进行了对应基因的克隆、功能验证、表达模式分析;
短句来源
    Through the study of data integration of proteome and gene, we can get plenty of data of the functional gene.
    蛋白质组数据与基因组数据的整合,将会在后基因组研究——基因组功能研究上取得十分丰富的数据资料。
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  functional gene
With the example of yeast genes, context organization was compared for functional gene regions (promoter, 5"-UTR, 3"-UTR) and tested for association with the level of gene expression.
      
Alternative ways take advantage of new extrachromosomal vector systems (pETE, pETR) and the functional gene inactivation test.
      
A full-size functional gene encoding the human ribosomal protein S21 was cloned and characterized.
      
The fertilized sea urchin eggs and dissociated embryonic cells at the blastula stage were treated with plasmids containing both the functional gene gal4 and the gene devoid of the regions encoding the activator domain.
      
The transfection of embryonic sea urchin eggs with the functional gene led to cell dedifferentiation and formation of tumor-like structures in the embryos or increased number of embryonic cells in culture.
      
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In silico cloning was a new strategy of gene cloning developed with the development of genome, EST projects and bioinformatics. Using wheat glucose 6 phosphate dehydrogenase cDNA (clone:Tagpdl) sequence as a querying probe, one highly homologous BAC clone sequence was obtained from rice sequence database of GenBank and the putative cDNA sequence of rice glucose 6 phosphate dehydrogenase was assembled according to the wheat clone. Futhermore, the full length cDNA of rice glucose 6 phosphate dehydrogenase...

In silico cloning was a new strategy of gene cloning developed with the development of genome, EST projects and bioinformatics. Using wheat glucose 6 phosphate dehydrogenase cDNA (clone:Tagpdl) sequence as a querying probe, one highly homologous BAC clone sequence was obtained from rice sequence database of GenBank and the putative cDNA sequence of rice glucose 6 phosphate dehydrogenase was assembled according to the wheat clone. Futhermore, the full length cDNA of rice glucose 6 phosphate dehydrogenase was cloned by RT PCR with two primers designed based on this assembled cDNA sequence. Since this fragment contained a complete ORF of 1515bp with a stop codon in its upstream and poly(A) signal in its downsteam, it could be concluded that a full length gene (GenBank accession number AY078072), which was named as OsG6PDH . Homology analysis of OsG6PDH showed a 88% identity with wheat and the deduced amino acid showed 89%, 79% and 80% omology with G6PDH from wheat, tomato and tobacco respectively. OsG6PDH was expressed in inflorescence, embryo, root and leaf of rice, with a slightly higher in inflorescence and root. It was also discussed in this paper that the application of in silico cloning in the isolation of functional genes from rice.

电子克隆是基因克隆的新策略。以小麦胞质葡萄糖 6 磷酸脱氢酶cDNA (Tagpdl克隆 )序列为信息探针 ,在GenBank水稻nr数据库中找到高度同源的水稻基因组序列 ,通过人工序列拼接及RT PCR确认得到了水稻该基因的全长cDNA序列 ,命名为OsG6PDH。OsG6PDH与小麦Tagpdl克隆的DNA一致率为 88% ,推导的氨基酸序列与小麦、番茄、烟草的胞质葡萄糖 6 磷酸脱氢酶基因的一致率分别为 89%、79%、80 %。经RT PCR表达谱分析 ,OsG6PDH在水稻幼穗、胚、根、叶中都有表达 ,在幼穗与根中表达略高。另外 ,讨论了利用水稻基因组信息的电子克隆方法克隆水稻功能基因的可行性

In silico cloning was a new way of gene cloning by bioinfomatics, which developed with genome enforcement and EST project. Since the rice draft sequence of genome was published in public, it was possible to isolate the functional genes of rice by in silico cloning. The principle, application, related resource of bioinformatics, problems and the prospect of in silico cloning of functional genes in rice were reviewed.

电子克隆是随着基因组计划和 EST计划实施发展起来的 ,利用生物信息学手段进行基因克隆的新方法。已经公布的水稻基因组序列框架图 ,使得水稻的电子克隆路线得以实施。介绍了水稻电子克隆的基本原另、应用实例、相关的生物信息资源、存在问题和展望

Sixteen SSR (simple sequence repeats) primers of functional genes in rice were used to detect genetic diversity among 23 rice germplasms from 5 countries in the world. The average number of alleles per SSR locus was 5.2 with a range from 2 to 10. Genetic similarities among the 23 rice accessions ranged from 0.13 to 0.64. UPGMA cluster analysis showed that the 23 rice accessions could be classified into two distinct classes at similarities coefficient 0.13. The Japonica rice from Brazil, Japan and China...

Sixteen SSR (simple sequence repeats) primers of functional genes in rice were used to detect genetic diversity among 23 rice germplasms from 5 countries in the world. The average number of alleles per SSR locus was 5.2 with a range from 2 to 10. Genetic similarities among the 23 rice accessions ranged from 0.13 to 0.64. UPGMA cluster analysis showed that the 23 rice accessions could be classified into two distinct classes at similarities coefficient 0.13. The Japonica rice from Brazil, Japan and China was classed into class Ⅰ and upland rice accessions from Brazil were classed into the same class. The Indica rice from Pakistan and Korea was classed into class Ⅱ. Consequently, function of gene's SSR markers could be used as a useful tool for measuring genetic diversity, assigning rice to geographical distribution, and analysing its ecotype and pedigree relationship.

利用 16对水稻功能基因的SSR引物研究了 2 3份世界 5个国家不同来源的水稻种质资源的遗传多样性 ,共检测出 78个等位基因变异 ,每对引物可检测 2~ 10个等位基因变异 ,平均为 5 .2个 ,品种间遗传相似系数在0 .13~ 0 .6 4之间。UPGMA聚类分析表明 ,在相似系数 0 .13处可以将材料分为 2大类 ,来自巴西、日本和中国的粳稻全部聚为第Ⅰ类 ,巴西陆稻和原产科特迪瓦的陆稻聚在第Ⅰ类的第三小群 ;第Ⅱ类全为籼稻 ,来源于巴基斯坦的籼稻和 1个来源于韩国的籼稻分布在第Ⅱ类 ,说明水稻功能基因在不同亚种、不同产地来源和不同生态类型的水稻之间存在差异 ,也进一步证实水稻功能基因的SSR标记是研究水稻种质资源分类、地理分布、生态类型和系谱分析的有效工具。

 
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