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functional gene
相关语句
  功能基因
    Progress on The functional gene of cell-cell signal transduction in self-incompatibility of Brassica oleracea L.
    甘蓝自交不亲和细胞信号转导的功能基因研究进展
短句来源
    THE FUNCTIONAL GENE CLONING DEVELOPMENT OF MICROFUNGUS
    大型真菌功能基因克隆研究进展
    With the diploid and a set of primary trisomics self-established as materials, in this research, cytological identification of genetic stability of the diploid and different trisomics via asexual multi-generation was made with chromosomal karyotype analysis, two rDNA repeat sequence and several functional gene fragments obtained by PCR and cloning were physically localized the specific chromosomes of Chinese cabbage with FISH technique.
    本研究以大白菜二倍体和本课题组创制的一套大白菜初级三体为材料,采用染色体核型分析的方法对继代繁殖多代的不同三体和二倍体的遗传稳定性进行了细胞学鉴定,采用FISH技术对两个rDNA重复序列基因和通过PCR扩增、克隆获得的几个功能基因片段进行了物理定位。 主要研究结果如下:
短句来源
  “functional gene”译为未确定词的双语例句
    The data are helpful to researches involving introducing a non-plant gene to citrus or vice versa and designing primers for functional gene cloning.
    分析结果对动物或微生物的基因在柑橘中的表达或柑橘基因在微生物中的表达以及基因克隆时设计引物具有一定指导意义。
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  functional gene
With the example of yeast genes, context organization was compared for functional gene regions (promoter, 5"-UTR, 3"-UTR) and tested for association with the level of gene expression.
      
Alternative ways take advantage of new extrachromosomal vector systems (pETE, pETR) and the functional gene inactivation test.
      
A full-size functional gene encoding the human ribosomal protein S21 was cloned and characterized.
      
The fertilized sea urchin eggs and dissociated embryonic cells at the blastula stage were treated with plasmids containing both the functional gene gal4 and the gene devoid of the regions encoding the activator domain.
      
The transfection of embryonic sea urchin eggs with the functional gene led to cell dedifferentiation and formation of tumor-like structures in the embryos or increased number of embryonic cells in culture.
      
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Fruit quality and pollen morphology from electron microscope scanning of‘Red Sun’kiwifruit and its red-fleshed sport (86-3) were studied. The results were as follows: The content of anthocyanin in 86-3 increased significantly and stayed stable in the consecutive two years, while other qualities were not so good as those of‘Red Sun’. The polar views, equatorial views and exine ornaments of pollen grains were similar between 86-3 and‘Red Sun’, while the length ratios of polar axis and equatorial axis(P/E) were...

Fruit quality and pollen morphology from electron microscope scanning of‘Red Sun’kiwifruit and its red-fleshed sport (86-3) were studied. The results were as follows: The content of anthocyanin in 86-3 increased significantly and stayed stable in the consecutive two years, while other qualities were not so good as those of‘Red Sun’. The polar views, equatorial views and exine ornaments of pollen grains were similar between 86-3 and‘Red Sun’, while the length ratios of polar axis and equatorial axis(P/E) were distinctively different. The study of pollen morphology indicated that there was a little difference in functional genes between 86-3 and its maternal plant.

对‘红阳’猕猴桃全红型芽变(863)及对照‘红阳’进行了品质及花粉电镜扫描方面的研究。结果表明,与‘红阳’相比,863果实中花青素含量极显著增加,变异性状稳定,但其它品质不及母株果实。两者花粉粒极面观、赤道面观及表面纹饰相似,而极轴长与赤道轴长之比(P/E)差异显著。花粉形态研究表明亲缘关系极近的芽变株及其母株的功能基因有所不同。

It is one important approach to clone functional genes.Vprdrf4,a cDNA fragment of the expression gene that differentially displays itself between non-inoculated leaves and the leaves inoculated with powdery mildew,was obtained from Baihe35-1 belonging to Vitis pseudoreticulata W.T.Wang.The fragment was verified by Northern hybridization to an up-regulated one and proved by Blast(Basic Local Alignment Search Tool) to be homogenous to the ribosomal RNA genes in eukaryotes at more than 90%,and...

It is one important approach to clone functional genes.Vprdrf4,a cDNA fragment of the expression gene that differentially displays itself between non-inoculated leaves and the leaves inoculated with powdery mildew,was obtained from Baihe35-1 belonging to Vitis pseudoreticulata W.T.Wang.The fragment was verified by Northern hybridization to an up-regulated one and proved by Blast(Basic Local Alignment Search Tool) to be homogenous to the ribosomal RNA genes in eukaryotes at more than 90%,and its GenBank accession code was CD347664.

克隆功能基因是研究植物基因结构及功能的重要途径.利用mRNA差异显示技术,筛选获得了中国葡萄属野生种华东葡萄白河-35-1在人工接种白粉菌前后差异表达基因的cDNA片段V prdrf4.经N orthern杂交验证,该片段为正调控片段,B last检索表明与真核生物核糖体RNA基因的同源性高达90%以上,G enB ank登录号为CD 347664.

177 coding DNA sequences (CDS) were used to analyze the relative frequency of synonymous codon (RFSC), and 7 high-frequency codons including TAA, GCT, GAT, CTT, AGG, AGA and GTT were revealed by high-frequency codon analysis. When the frequency of codon usage of citrus was compared to Homo sapiens, Drosophila melanogaster, Saccharomyces cerevisiae and Escherichia coli, we found that the codon preference is considerably different between citrus and non-plant species. While compared to Arabidopsis thaliana, Lycopersicon...

177 coding DNA sequences (CDS) were used to analyze the relative frequency of synonymous codon (RFSC), and 7 high-frequency codons including TAA, GCT, GAT, CTT, AGG, AGA and GTT were revealed by high-frequency codon analysis. When the frequency of codon usage of citrus was compared to Homo sapiens, Drosophila melanogaster, Saccharomyces cerevisiae and Escherichia coli, we found that the codon preference is considerably different between citrus and non-plant species. While compared to Arabidopsis thaliana, Lycopersicon esculentum, Oryza sativa and Musa acuminata, we found that the codon preference was identical in dicotyledons, but was different between citrus and monocotyledon, Oryza sativa and Musa acuminate. The data are helpful to researches involving introducing a non-plant gene to citrus or vice versa and designing primers for functional gene cloning.

采用高频密码子分析法,对柑橘的177个蛋白质编码基因序列(codingDNAsequence,CDS)进行了分析,计算出同义密码子相对使用频率(relativefrequencyofsynonymouscodon,RFSC),确定了TAA、GCT、GAT、CTT、AGG、AGA和GTT等7个高频密码子。将柑橘的密码子使用频率与人、果蝇、酵母和大肠杆菌等不同种类模式生物比较后发现,柑橘密码子的偏爱性与不同种类生物有不同程度的差异;但将柑橘的密码子使用频率与拟南芥、番茄、水稻和尖叶蕉等不同种类的植物相比,发现柑橘密码子的偏爱性与同为双子叶植物的拟南芥、番茄完全一样,而与水稻、尖叶蕉这2种单子叶植物均有较大的差异。分析结果对动物或微生物的基因在柑橘中的表达或柑橘基因在微生物中的表达以及基因克隆时设计引物具有一定指导意义。

 
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