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functional gene
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  功能基因
    Screening the functional gene cDc42 - like protein from adult clonorchis sinensis with bio - information methods.
    应用生物信息学方法筛选华支睾吸虫功能基因-Cdc42样蛋白
短句来源
    The Research Progress on Stage-difference Expression of Functional Gene in Schistosome
    血吸虫期别差异表达功能基因的研究进展
短句来源
    Screening of Calcineurin B-like Protein Gene,The Functional Gene of Adult Clo norchis sinensis by Bioinformatical Method
    生物信息学方法筛选华支睾吸虫成虫功能基因——钙调神经磷酸酶B亚基样蛋白
短句来源
    The results showed that the primary variations were obtained in profile of some functional gene expressions of bone marrow cells in irradiated mice.
    初步得到辐射损伤后骨髓细胞某些功能基因的差异表达变化谱 ;
短句来源
    Aim: To explore the molecular mechanism of heat stress by isolating diff erentially expressed genes occurred during the course of heat stress and filtrat ing functional gene segments concerned with heat stress with Suppression Subtrac tive Hybridization technique.
    目的 :应用抑制性消减杂交技术 ,分离热应激过程中出现的差异表达基因 ,筛选与热应激相关的功能基因片断 ,探索热应激的分子机理。
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  “functional gene”译为未确定词的双语例句
    Site-directed mutagenesis of functional gene fragment producing CTX-M-14-type extended-spectrum β-lactamase
    CTX-M-14型超广谱β-内酰胺酶基因的定点突变研究
短句来源
    Conclusion:It was suggested that obtained VH gene was potentially functional gene of the McAb COC183B2 against human ovarian carcinoma
    结论:该VH基因为抗人卵巢癌单抗COC183B2功能性重链可变区基因。
短句来源
    [Conclusion] The obtained V L gene was a potentially functional gene of anti idiotypic monoclonal antibody NP30 of Schistosoma japonicum .
    [结论 ]该VL 基因为日本血吸虫单克隆抗独特型抗体NP30轻链可变区基因。
短句来源
    Conclusion: The authors cloned the MHC Ⅰ moleculur antigen recognizing gene (H-2K+k ) of 615 mice successfully and got the functional gene of MHC Ⅰ.
    结论 :成功克隆了 6 15小鼠MHCⅠ分子抗原识别基因 ,获得了表达MHCⅠ功能的目的基因
短句来源
    Study on Variation and Clinic of Functional Gene of HBV of the Diverse-Race Patients with Chronic Hepatitis B from Ethnic Minority Areas in Yunnan
    云南少数民族地区乙型肝炎病毒功能基因变异的研究
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  functional gene
With the example of yeast genes, context organization was compared for functional gene regions (promoter, 5"-UTR, 3"-UTR) and tested for association with the level of gene expression.
      
Alternative ways take advantage of new extrachromosomal vector systems (pETE, pETR) and the functional gene inactivation test.
      
A full-size functional gene encoding the human ribosomal protein S21 was cloned and characterized.
      
The fertilized sea urchin eggs and dissociated embryonic cells at the blastula stage were treated with plasmids containing both the functional gene gal4 and the gene devoid of the regions encoding the activator domain.
      
The transfection of embryonic sea urchin eggs with the functional gene led to cell dedifferentiation and formation of tumor-like structures in the embryos or increased number of embryonic cells in culture.
      
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Objective: To amplify and sequence the heavy chain variable region (VH ) gene of monoclonal antibody (McAb) COC183B2 against human ovarian carcinoma. Methods: The VH gene of mouse McAb COC183B2 against human ovarian carcinoma was amplified by RTPCR . The PCR product was then cloned into PUC19 vector. The recombinants were sequenced by Sanger's method. The VH gene was compared with Gene Bank and published mouse VH genes. Results: It was proved that a fulllength VH gene was 354 bp, the VH gene was...

Objective: To amplify and sequence the heavy chain variable region (VH ) gene of monoclonal antibody (McAb) COC183B2 against human ovarian carcinoma. Methods: The VH gene of mouse McAb COC183B2 against human ovarian carcinoma was amplified by RTPCR . The PCR product was then cloned into PUC19 vector. The recombinants were sequenced by Sanger's method. The VH gene was compared with Gene Bank and published mouse VH genes. Results: It was proved that a fulllength VH gene was 354 bp, the VH gene was a member of mouse Ig heavy chain subgroup miscellaneous and originated from rearrangement of VH、 Dsp2.5 and MUSJH4 gene. The VH gene sequence was registered by Gene Bank(accession No. AF045023). Conclusion:It was suggested that obtained VH gene was potentially functional gene of the McAb COC183B2 against human ovarian carcinoma

目的:分离抗人卵巢癌单克隆抗体COC183B2重链可变区(VH)基因并测定其序列。方法:用反转录PCR扩增抗人卵巢癌单抗COC183B2VH基因,将其克隆入PUC19载体,重组子用Sanger’s双脱氧链终止法测定序列,将序列与GeneBank及已发表的抗体序列比较。结果:VH基因全长354bp,属鼠免疫球蛋白重链混杂亚类(subgroupmiscelaneous),由种系基因的VH,Dsp2.5及MUSJH4重排而来。该VH基因序列已被GeneBank收录(accessionNoAF045023)。结论:该VH基因为抗人卵巢癌单抗COC183B2功能性重链可变区基因。

Aim To clone and analyze variable region genes from an anti transferrin receptor(anti TfR) monoclonal antibody (line 7579) Methods From a secreting mouse hybridoma cell line 7579, total RNA was prepared and was reverse transcribed into the first strand of cDNAs The V H and V L genes were amplified by PCR with family specific primers, and then cloned into the pGEM T vectors in the competent cell JM109 respectively With the dye terminator cycle sequencing method, the V H and V L...

Aim To clone and analyze variable region genes from an anti transferrin receptor(anti TfR) monoclonal antibody (line 7579) Methods From a secreting mouse hybridoma cell line 7579, total RNA was prepared and was reverse transcribed into the first strand of cDNAs The V H and V L genes were amplified by PCR with family specific primers, and then cloned into the pGEM T vectors in the competent cell JM109 respectively With the dye terminator cycle sequencing method, the V H and V L were sequenced The results were analyzed with DNASIS7, and also were compared with the NIH′s GenBank Results: The V H and V L were respectively consisted of 139 and 128 amino acid residues with signal peptides, CDRs and FRs Conclusion: The V H and V L were the full length and potentially functional genes from the monoclonal anti TfR antibody secreting hybridoma

目的:制备基因工程抗体,减少或消除鼠源性单克隆抗体(mAb)在人体内的免疫原性,保留其对人体抗原配体的高度特异性,发展临床导向诊断和治疗。方法:从体外分泌抗转铁蛋白受体mAb杂交瘤细胞系7579中,对其mAb可变区基因进行克隆和序列分析。利用逆转录PCR技术扩增轻、重链可变区基因,再分别与pGEMT载体连接,并克隆于JM109受体菌之中。利用荧光染色链终止法测定其序列,采用DNASIS7分析软件和与NIH基因库比较分析。结果:轻、重链可变区分别由139和128氨基酸残基组成,包括信号肽、互补决定区和骨架氨基酸残基等功能序列。分别属于鼠免疫球蛋白重链Ⅱc和κ链Ⅵ家族。结论:来自mAb可变区的基因是完整的和具有潜在功能性的,为体外获得抗转铁蛋白受体基因工程抗体奠定了基础。

Objective To determine whether an exogenous gene carried by lipofectin can be introduced into the primary human lens epithelial cells. Methods Plasmid DNA with betagalactosidase gene carried by lipofectin was applied to primary cultured human lens epithelial cells.Gene expression was detected by enzymatic color reaction using Xgal as a substrate in the 2 and 6 days of expression after 12, 24, 36 hours of transfection respectively. The gene transfer positive rate of the lens epithelial cells was counted....

Objective To determine whether an exogenous gene carried by lipofectin can be introduced into the primary human lens epithelial cells. Methods Plasmid DNA with betagalactosidase gene carried by lipofectin was applied to primary cultured human lens epithelial cells.Gene expression was detected by enzymatic color reaction using Xgal as a substrate in the 2 and 6 days of expression after 12, 24, 36 hours of transfection respectively. The gene transfer positive rate of the lens epithelial cells was counted. Results The foreign gene could be transferred into primary human lens epithelial cells by lipofectin. In the expression of 2 days, transfer positive rate was up to 48% after 24 hours of transfection. Conclusions Efficient and stable transfer of the functional gene can be achieved by lipofectin into the lens epithelial cells. Lipofectin is an available and a promising vehicle for delivering aim gene and studying on the mechanism of physiology and pathology of lens epithelial cells.

目的确定外源基因能否由脂质体携带进入人原代晶体上皮细胞(primaryhumanlensepithelialcels,PHLECs)。方法体外培养人原代晶体上皮细胞,用阳离子脂质体(lipofectinreagent,LR)携带重组质粒报道基因(β半乳糖苷酶),向人原代晶体上皮细胞转移,在转移12、24、36小时,分别表达2天和6天时,用以Xgal为底物的酶显色方法,检测报道基因对人原代晶体上皮细胞的转移率。结果脂质体(lipofectin)介导的外源基因可以转入人原代晶体上皮细胞,在转移24小时,表达2天时转移率可达48%。结论稳定的外源基因可由脂质体介导转入生长中人原代晶体上皮细胞,作为晶体上皮细胞基因类药物介入的基础研究和应用研究的介导体,脂质体显示出有希望的前景。借此方法也可以从外源基因入手,对晶体上皮细胞的生理和病理活动,进行机制研究。

 
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