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functional gene
相关语句
  功能基因
    Conclusion: ZTF was the effective composition of LCD, and its mechanism was associated with the regulation of several functional gene and protein.
    结论:ZTF为珍珠菜抗肿瘤有效部位,其抗肿瘤作用机制与其对多种功能基因及蛋白的调控并导致细胞凋亡相关。
短句来源
    The altered gene profile of human osteosarcoma cell after knock-down of double functional gene apurinic/apyrimidinic endonuclease
    双功能基因APE1敲低后人体骨肉瘤细胞基因表达谱变化的研究
短句来源
    Following the finish of the plan of human genome and the biginning of the study of the functional gene, more and more genes were used to treat human disease.
    随着人类基因组计划的完成和功能基因研究的开展,可用于基因治疗的功能基因越来越多。
短句来源
    The gene therapy must solve three key questions to obtain the breakthrough: the highly effective gene delivery system, the controllability of gene expression and the better functional gene.
    同时,利用此载体平台还可将本实验的检测基因(luciferase)换为任意的功能基因,以便使增加了靶向性的腺病毒载体能更好的应用于肿瘤的基因治疗。
短句来源
    Conclusion The fusion protein H1s-EGFc binds functional gene efficiently and targets it into specific cells. It can be used as non-viral vector in target cancer gene therapy.
    而对表皮生长因子受体不表达的细胞无杀伤作用。 结论 融合蛋白H1s EGFc能够有效地用于功能基因的包装及转运 ,可以作为非病毒载体应用于恶性肿瘤的靶向性基因治疗。
短句来源
  “functional gene”译为未确定词的双语例句
    Meanwhile Bioinformatics has played an important role in the study of functional gene identification, illness diagnosis, gene expression profiling and protein function.
    同时,随着人类基因组计划进一步的快速发展,生物信息学在人类疾病与功能基因的发现与识别、基因与蛋白质的表达与功能研究方面都发挥着关键的作用。
短句来源
    Conclusion The obtained VL gene is potentially the functional gene of the McAb COC183-B2 against human ovarian carcinoma. 
    结论该VL基因为抗人卵巢癌单抗COC183-B2功能性轻链可变区基因。
短句来源
    CONCLUSION The obtained VL gene is potentially the functional gene of the McAb against human laryngeal carcinoma.
    结论该基因为抗人喉癌单克隆抗体LC9-G5轻链可变区基因。
短句来源
    Both of samples were labeled with fluorescent Cy5 or Cy3, and then hybridized to the gene chip which it includes 14 112 human functional gene fragments.
    方法:分别用Cy3和Cy5两种荧光染料标记两组组织的总mRNA,与含有14112个基因位点的芯片进行杂交。
短句来源
    Being the kernel of functional gene group,the field of protein group becomes the focal point of life science research in 21th century.
    蛋白质组学是21世纪生命科学研究领域的热点。
短句来源
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  functional gene
With the example of yeast genes, context organization was compared for functional gene regions (promoter, 5"-UTR, 3"-UTR) and tested for association with the level of gene expression.
      
Alternative ways take advantage of new extrachromosomal vector systems (pETE, pETR) and the functional gene inactivation test.
      
A full-size functional gene encoding the human ribosomal protein S21 was cloned and characterized.
      
The fertilized sea urchin eggs and dissociated embryonic cells at the blastula stage were treated with plasmids containing both the functional gene gal4 and the gene devoid of the regions encoding the activator domain.
      
The transfection of embryonic sea urchin eggs with the functional gene led to cell dedifferentiation and formation of tumor-like structures in the embryos or increased number of embryonic cells in culture.
      
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Objective:To analyze the variable region sequences of an antiidiotypic monoclonal antibody to human chronic B cell leukemia (BCLL). Methods:The variable region sequences of light and heavy chains (VL、VH) of antiidiotypic McAb SM6 to BCLL were analyzed by their cDNA cloning and sequencing. Total RNA was extracted from a hybridoma cell line producing SM6. The VH and VL genes were amplified by RTPCR with specific primers. The PCR products were cloned into pGEMT vectors, then transfected into JM109....

Objective:To analyze the variable region sequences of an antiidiotypic monoclonal antibody to human chronic B cell leukemia (BCLL). Methods:The variable region sequences of light and heavy chains (VL、VH) of antiidiotypic McAb SM6 to BCLL were analyzed by their cDNA cloning and sequencing. Total RNA was extracted from a hybridoma cell line producing SM6. The VH and VL genes were amplified by RTPCR with specific primers. The PCR products were cloned into pGEMT vectors, then transfected into JM109. Results and Conclusion: It was confirmed by DNASIS7 sequence analysis that the fulllength and potentially functional genes were successfully cloned. The VH gene expressed in SM6 belongs to Q52, the JH region using the JH3. The VL gene belongs to VK19, the JK region using JK2.

目的:对独特型抗体可变区基因进行序列分析,为其基因工程抗体的构建打下基础。方法:采用TRIzolR试剂从分泌抗独特型抗体SM6杂交瘤细胞株中提取mRNA,以特异性引物采用PCR技术扩增并克隆出独特型单抗SM6重、轻链可变区(VH、VL)基因。将VH和VL基因分别克隆到pGEMT载体中。采用链末端终止法荧光染色测定VH和VL基因序列,应用DNASIS7分析软件并与美国国立卫生研究院基因库比较分析。结果:5个克隆的重链基因序列一致,3个克隆的轻链基因中有2个克隆基因一致。结论:VH和VL基因分别属Q52、JH3和VK19、JK2。

Objective To amplify and sequence the light chain variable region (VL) gene of monoclonal antibody (McAb) COC183-B2 against human ovarian carcinoma.Methods The VL gene of mouse McAb COC182-B2 against human ovarian cracinoma was amplified by PCR. The PCR product was then cloned into PUC19 vector. The recombinants were sequenced by Sanger's method. The VL gene was compared with published mouse VL genes of Gene Bank, EMBL, DDBJ and PDB via Internet. Results It was proved that a full-length VL gene...

Objective To amplify and sequence the light chain variable region (VL) gene of monoclonal antibody (McAb) COC183-B2 against human ovarian carcinoma.Methods The VL gene of mouse McAb COC182-B2 against human ovarian cracinoma was amplified by PCR. The PCR product was then cloned into PUC19 vector. The recombinants were sequenced by Sanger's method. The VL gene was compared with published mouse VL genes of Gene Bank, EMBL, DDBJ and PDB via Internet. Results It was proved that a full-length VL gene was 336 bp, the VL gene was a member of mouse immunoglobulin (Ig) light chain subgroup Ⅱ and originated from rearrangement of VK and MUS JK2 gene. The VL gene sequence was registered by Gene Bank (accession No.gb AF 044077).Conclusion The obtained VL gene is potentially the functional gene of the McAb COC183-B2 against human ovarian carcinoma.

目的分离抗人卵巢癌单克隆抗体COC183-B2轻链可变区(VL)基因并测定其核苷酸序列。方法用PCR方法体外扩增单抗COC183-B2VL基因,将其克隆入PUC19载体,重组子用Sanger’s双脱氧链终止法测定其核苷酸序列,将其序列通过Internet与GeneBank、EMBL、DDBJ和PDB已发表的抗体序列进行比较分析。结果VL基因全长336bp,属小鼠免疫球蛋白轻链第Ⅱ亚类(SubgroupⅡ),由种系基因的VK及J基因的MUSJK2重排而成。该VL基因序列已被GeneBank收录(acesionNo.gbAF044077)。结论该VL基因为抗人卵巢癌单抗COC183-B2功能性轻链可变区基因。

Objective This experiment aims at enhancing the efficiency of cationic liposome-mediated gene transfer to cultured lung cancer cells. Methods Reporter gene expressing E.coli β-galactosidase were used in order to evaluate transfection efficiency by histochemical staining. Optimization of conditions for cationic liposome-mediated transfection of cultured lung cancer cells include: charge ratios of cationic liposome: pCMVβ(+/-),doses of transferred pCMVβ, and the types of liposomes. Results When...

Objective This experiment aims at enhancing the efficiency of cationic liposome-mediated gene transfer to cultured lung cancer cells. Methods Reporter gene expressing E.coli β-galactosidase were used in order to evaluate transfection efficiency by histochemical staining. Optimization of conditions for cationic liposome-mediated transfection of cultured lung cancer cells include: charge ratios of cationic liposome: pCMVβ(+/-),doses of transferred pCMVβ, and the types of liposomes. Results When the cationic liposome: pCMVβ charge ratio is 3∶1 and dose D(Per well of 24-well plate)/ μg of pCMVβ is 4, the highest efficiency of DOSPER mediated gene transfer into SPC-A-1 cell reachs(11 9±0 9)%. Conclusions Optimising the conditions of transfection, cationic liposomes can effectively transfer the gene into cultured lung cancer cells.The results lay the fundations of functional gene transfer to lung cancer cells and further to the gene therapy of lung cancer.

【目的】提高阳离子脂质体介导的肺癌细胞基因转染效率。【方法】采用表达E .coliβ 半乳糖苷酶的 pCMVβ报告基因质粒及组化染色评价阳离子脂质体的转染效率。优化脂质体介导肺癌细胞基因转染效率的条件包括 :脂质体∶DNA电荷比率 ,DNA转染剂量 ,阳离子脂质体的类型。【结果】当阳离子脂质体∶DNA电荷比率 (+ / - )为 3∶1时 ,pCMVβ 的剂量为4μg/孔 (2 4孔板 )时 ,阳离子脂质体DOSPER介导肺癌细胞株SPC A 1转染效率最高 ,达 (11 9± 0 9) %。【结论】优化转染条件 ,脂质体可有效地将外源基因体外导入肺癌细胞。本实验为阳离子脂质体介导肺癌细胞功能性基因的转移及进一步的肺癌基因治疗打下了基础。

 
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