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anti insulin
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  抗胰岛素
     Finally,we used anti insulin antibody to react with the encapsulated insulin,and this led to capture of the nanoparticles as well,which could be detected by scanning electron microscope (SEM).
     用抗胰岛素抗体与包裹胰岛素的颗粒反应,可以在电镜下观察到包裹颗粒被抗体捕获。
短句来源
     Conclusion The effects of hyperglycemia on calcium load and cyclic AMP content in skeletal muscle may accord with its anti insulin action,which may be involved in the mechanism of glucose toxicity.
     结论 高血糖对大鼠骨骼肌钙负载和 c AMP含量的影响与其抗胰岛素作用相一致 ,可能与葡萄糖毒性的发生有关
短句来源
     All ICA positive sera were classified according to the patterns of ICA staining on islet. Double immunostaining of patient's serum and anti glucagon antibody or anti insulin antibody were applied for identification of islet cell types stained by different ICA subsets.
     根据ICA染色形态对全部ICA阳性血清进行亚型划分 ,再用患者血清和抗胰高血糖素抗体或抗胰岛素抗体进行免疫双重染色 ,鉴定不同ICA亚型着染的胰岛细胞类型。
短句来源
  “anti insulin”译为未确定词的双语例句
     Anti insulin sera are obtained from male guinea pigs immunized with porcine insulin and are used for preparation of affinity chromatographic column.
     介绍了亲和层析法制备胰岛素零值血清和胰岛素放射免疫分析质控血清研制的过程。
短句来源
     The column is packed with CNBr activated Sepharose 4B coupling with anti insulin sera. When normal human sera are passing through the affinity chromatographic column, insulin is adsorbed on the column.
     胰岛素为免疫原对豚鼠进行免疫获得抗血清,将此抗血清直接偶联到溴化氰活化Sepharose4B凝胶上,制成亲和层析柱,使正常人血清通过此柱,胰岛素被特异地吸附在柱中而从血清中除掉。
短句来源
     Reverse phase evaporation (REV)method to prepare large unilamellar vesicles(LUVs)encapsulated sulforhodamine B,and obtained immunoliposome by using 1 ethy1 3(3 dimethylaminopropy1)carbodiimide(EDCI)which made insulin couple the LUVs. Competing inhibition assay could be achieved upon the addition of free insulin and anti insulin antibody in the presence of complement.
     应用逆相蒸发法(REV)制备包裹有丽丝胺罗丹明B的大单层脂质体(LUVs),通过碳二亚胺(EDCI)法偶联胰岛素抗原,当加入胰岛素抗原、一定量抗体,在补体参与下,建立竞争抑制测定模式。
短句来源
     Methods:By checking up the blood sugar and the insulin for the menopauce patients,who took the oral glucose tolerance test(OGTT) and insulin release test in fast state and two hours after the meal,who were divided into two groups,one group including 27 cases(B group) and the other 14 cases(A group,control group),the index of anti insulin(IAI) and the index of anti insulin resistance(HOMAIR) were counted and compared with each other.
     方法 :通过对绝经后妇女 (B组 ) 2 7例及对照组 (A组 ) 1 4例进行口服葡萄糖耐量试验 (OGTT)及胰岛素释放试验 ,测定其空腹、餐后两小时的血糖及胰岛素值 ,计算胰岛素敏感性指标 (IAI)及胰岛素抵抗指数 (HOMA IR)并进行比较。
短句来源
     The assay results were expressed by IAA index and compared with those of an international standardized laboratory and a domestic anti insulin antibody kit. Sera of 32 recent onset type 1 diabetic patients and 120 healthy controls were screened for IAA and the consistency, sensitivity, specificity and the diagnostic value of IAA were evaluated.
     通过与国际标准化实验室及国内商品化IAA放射免疫试剂盒检测结果比较 ,并检测 1 2 0例健康对照和 32例初发T1DM患者IAA水平 ,评价其一致性、灵敏度和特异性 ,以及临床应用价值。
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  相似匹配句对
     Anti-S.
     体外模拟试验发现抗S.
短句来源
     Anti-H.
     治疗结果显示H.
短句来源
     insulin ;
     insulin ;
短句来源
     ANTI-INSULIN ANTIBODY DETERMINATION AND ITS CLINICAL APPLICATION
     胰岛素抗体的测定和临床应用
短句来源
     THE INTERACTION OF INSULIN FRAGMENTS WITH ANTI-INSULIN SERUM
     胰岛素片段与胰岛素抗体的相互作用
短句来源
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  anti insulin
The fabricated micro sensor was tested by detecting the mussel gluing protein, the insulin-anti insulin binding protein and the poly T-sequence DNA.
      
The anti insulin effects of pituitary extracts and cortisone have been subject to wide study.
      
The characteristics ofthis monoclonal anti-insulin receptorantibody are the subject ofthis report.
      
There has been little work on the effect of anti-insulin antibodies in immunometric assays.
      
Theinsulin response wasapparent after30 min and was completely inhibited by the addition of guinea pig anti-insulin antiserum to the incubationflasks.
      
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A valid method for the determination of specific radioactivity of A14-[~(125)I] monoiodoinsulin with self-displacement technique was described.The importance of antibody in this method was stressed in that, the radio-iodinated tracer and the native antigen must have a same binding affinityto the antibody used .From 28 species of anti-insulin antiserum screened,six of them fulfil this condition .The slope of standard curves on semilo-garithmic basis was used as parameter to compare the binding affinitiesof...

A valid method for the determination of specific radioactivity of A14-[~(125)I] monoiodoinsulin with self-displacement technique was described.The importance of antibody in this method was stressed in that, the radio-iodinated tracer and the native antigen must have a same binding affinityto the antibody used .From 28 species of anti-insulin antiserum screened,six of them fulfil this condition .The slope of standard curves on semilo-garithmic basis was used as parameter to compare the binding affinitiesof a antiserum versus noniodinated and A14 iodinated insulin standards. Some practical problems concerning the application of self-displacementmethod have also been studied.

本文报告了一种筛选合适抗血清的简便方法。用此法对28株豚鼠抗胰岛素抗血清进行了检测,将其中典型的两株,以自身置换法测定了两批A14—[~(125)碘]单碘原子碘化胰岛素的比放射活性。本文着重探讨了抗血清的重要性,并对自身置换法应用中的若干要点进行了讨论。

Using two different guinea pig anti-insulin antisera with different an-tigen determinants versus two insulin analogues, native insulin and A14-(~(125)I) monoiodoinsulin,it was able to demonstrate in a RIA system thatthe iodination of A14 tyrosine residue of insulin molecule has decreasedthe binding affinity of one of the antisera (D7), but not the other one(GP12).Furthermore, this effect, as evidenced by Scatchard analysis andcomparing the slope values of different parts of standard...

Using two different guinea pig anti-insulin antisera with different an-tigen determinants versus two insulin analogues, native insulin and A14-(~(125)I) monoiodoinsulin,it was able to demonstrate in a RIA system thatthe iodination of A14 tyrosine residue of insulin molecule has decreasedthe binding affinity of one of the antisera (D7), but not the other one(GP12).Furthermore, this effect, as evidenced by Scatchard analysis andcomparing the slope values of different parts of standard curves, presentedonly in the first half of the standard curves using D7.This suggests thatonly the predominating functioning populations of antibodies of D7 withhigh binding affinity were involved, but not those with low binding af-finity. Accordingly, A19 iodination of insulin molecule might lower theinherent immunoreactivity of the predominating antibody populations ofGP12 with low binding affinity. The significance of this phenomenon wasstressed that a radioiodinated tracer might have different bindingaffinity compared to native antigen against certain specific antisera aswell as specific receptors.Therefore, it is not likely that every antiserumwould be suitable for a RIA system designated for special purposes likeself-displacement. To solve this problem the author suggested a simplemethod to select applicable antisera by comparing the slope values of stand-ard curves using native antigen and"cold"iodinated antigen which is chem-ically similar to the tracer used.

本文用原型胰岛素和A14“冷”单碘原子碘化胰岛素作标准品,同时用两株不同的豚鼠抗胰岛素抗血清作放射免疫分析。其结果经Scatchard分析和比较标准曲线斜率的方法证明,胰岛素分子A链第14位酪氨酸的碘标记,干扰其中一株抗血清(D7)固有的抗原抗体结合,降低其免疫活性,而对另一株抗血清(GP12)没有影响。此现象的意义在于进行自身置换分析等特殊的放射免疫分析时,必须考虑到~(125)碘标记对免疫活性或生物活性的干扰问题。

Using anti-porcine proinsulin guineapig serum and(~(126)I )-porcine proinsulin,a radioimmunoassay for determinationof proinsulin in insulin preparationshas been set up and evaluated. Themethod involves the saturation of anti-insulin antibodies with surplus insulinin the assay system and use of 25%polyethylene glycol as the separatingreagent Tracers were prepared in thislaboratory by LPO or ChT iodination,followed by polyacrylamide long geldisk electrophoretic purification. Theantiserum...

Using anti-porcine proinsulin guineapig serum and(~(126)I )-porcine proinsulin,a radioimmunoassay for determinationof proinsulin in insulin preparationshas been set up and evaluated. Themethod involves the saturation of anti-insulin antibodies with surplus insulinin the assay system and use of 25%polyethylene glycol as the separatingreagent Tracers were prepared in thislaboratory by LPO or ChT iodination,followed by polyacrylamide long geldisk electrophoretic purification. Theantiserum tised, G8304, was selectedfrom ten species of antisera immunizedwith porcine proinsulin (Novo). The Kaof G8304 was 3.05× 10~9 LM~(-1) at1: 40, 000 dilution, which is comparablewith that of GP114 (9.02×10~9 LM~(-1) ),another antiserum obtained from Hage-dorn Research Laboratory, Gentofte,Denmark. The standard ranges from 1to 256 ng/ml. The quality parametres for the RIAwere: within-assay variance RER 3.3%to 4.9%, recovery 93.4± 0.07% at 15ng/ml or 92.5±0.17% at 5.4ng/ml andsensitivity 0.5ng/ml for GP114 or 1.0ng/ml for G8304. Variances of deter-mination during sample dilution werealso tested. Some marketed domestic and import-ed insulin preparations were assayed.The results showed. fhat the proinsulincontent for domestic insulins was muchhigher than that of the imported insulinby some 300-fold. The results for someof the laboratorymade insulin prepara-tions prepared by the School ofPharmacy of this College haveconfirmed the observation that thecontaminating proinsulin in insulinpreparation could be satisfactorilyeliminated from insulin by gel chroma-tography to such an extent that theremaining content of proinsulin wasnot much higher than that in importedpreparation.

本文报道一种测定胰岛素制剂中猪胰岛素原含量的放射免疫分析法。本法以[~(125)I]猪胰岛素原为示踪物,用过量胰岛素封闭抗血清中的胰岛素抗体,以聚乙二醇作分离剂,建立了特异性猪胰岛素原放免分析系统。经检验其精确性、准确性、灵敏度和特异性,均符合科研要求。

 
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