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   bovine viral 的翻译结果: 查询用时:0.01秒
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bovine viral
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  牛病毒性
     The P20 and P14 protein genes of bovine viral diarrhea virus(BVDV) were subcloned into two prokaryotic expression vector,pGEX-6p-1 and pPROEX-HTb,respectively,to construct recombinant expression plasmids. The recombinant plasmids were transformed into competent BL21(DE3)pLysS and DH5α accordingly for expression.
     将牛病毒性腹泻病毒(bovine viral diarrhea virus,BVDV)p20和p14基因分别亚克隆至原核表达载体pGEX-6p-1和pPROEX-HTb,并转化至相应的宿主菌大肠杆菌BL21(DE3)pLysS和DH5α中表达。
短句来源
     Cloning and sequence analysis of the P20 and P14 gene of bovine viral diarrhea virus
     牛病毒性腹泻病毒P20和P14基因的克隆及序列分析
短句来源
     The E_0 gene of bovine viral diarrhea virus in Sika deer was amplified with RT-PCR,cloned into Cloning vector pMD18-T and prokaryotic expression vector pET28a,sequenced and expressed.
     利用RT-PCR技术扩增了牛病毒性腹泻病毒(BVDV)梅花鹿分离株E0基因,并将其克隆到pMD18-T克隆载体和pET28a原核表达载体中,构建了pMD18-T/E0和pET28a/E0重组子,并进行了测序和表达。
短句来源
     In order to study the biological functions of Erns gene of bovine viral diarrhea virus(BVDV), pMD18-T-Erns was digested with Bam H I and HindⅢ,and Erns gene fragment was obtained. The Erns gene was subcloned into the baculovirus transfer vector pBlueBacHis2A,and the recombinant plasmid pBlueBacHis2A-Erns was constructed.
     为研究牛病毒性腹泻病毒(BVDV)Erns基因的生物学功能,将含有牛病毒性腹泻病毒 Erns基因的质粒pMD18-T-Erns经BamH I/HindⅢ双酶切,获得了Erns片段,再与杆状病毒转移载体pBlueBacHis2A连接,构建成重组质粒。
短句来源
     Cloning of E2 Gene of Bovine Viral Diarrhea Virus Strain of China and Study on Antigenic Specificity of Recombinant E2 Protein
     牛病毒性腹泻病病毒中国毒株E2基因的鉴定及表达E2蛋白的抗原性研究
短句来源
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  “bovine viral”译为未确定词的双语例句
     Cloning and prokaryotic expression of the E1 gene of bovine viral diarrhea virus
     牛粘膜病病毒E1基因的克隆及原核表达
短句来源
     Study on the prevention of bovine viral diarrhoea-mucosal disease of the yak
     牦牛病毒性腹泻/粘膜病的防制研究
短句来源
     The major antigenic coding gene E2 of the earliest Chinese isolates of bovine viral diarrhea virus,bovine isolate Changchun184(CC_184) was obtained by use of reverse transcription polymerase chain reaction (RT_PCR)and nest_PCR. Sequencing result showed that the E2 gene of CC_184 has 1 122 nucleotides in length,which encode 374 amino acid residues.
     根据GenBank已发表的多个BVDV_1序列的比较分析结果设计引物 ,应用RT_PCR及套式PCR克隆得到包含Changchun184 (CC_184 )株E2基因的片段F2 /R2 ,克隆、测序分析结果表明该片段大小为 1391bp ,软件分析结果表明CC_184株E2基因长度为 112 2bp(GenBankaccessionnumber:AF5 2 6 380 )。
短句来源
     Bovine viral diarrhoea-Mucosal disease virus ( BVD-MDV ) antigens were detected with a double antibody sandwich enzyme-linked immunosorbent assay(ELISA). The results revealed that Oregon C24V P/N:3.141,the isolated strain P/N :3.012,P/N>2,it was identical with the standard strain of Oregon C24V. Observed by negative staining electron microscope, the virus could be seen with typical MDV virions.
     双抗体夹心ELISA检测病毒抗原结果BVDVOregonC24VP/N为3.141,分离毒P/N为3.012,P/N>2,与BVDVOregonC24V结果一致;
短句来源
     Alignment of with E2 sequences of pestivirus CSFV,BVDV(bovine viral diarrhea virus) and BDV(border disease virus) pointed A11 core sequence to 28-35 amino acid location of E2 protein although sequence identity is low. Chemically synthesized peptide containing 30 to 42 amino acid region of E2 could react with A11 further indicating the 28-35 amino acid region is a novel epitope of CSFV.
     多序列比较发现,该核心序列与猪瘟病毒E蛋白的28~35位氨基酸TTW KEY SH有一定的同源性,人工合成的含有部分核心序列氨基酸的多肽可以与单抗A 11反应,表明单抗A 11所针对的抗原表位位于CSFV E 2蛋白的28~35位氨基酸。
短句来源
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  相似匹配句对
     Bovine Viral Dianrhoea—Mucosal Disease
     牛病毒性腹泻—粘膜病的诊断,病原分离和予防研究
短句来源
     Maturation and Release of Bovine Viral Diarrhea Virus
     牛病毒性腹泻病毒的成熟和释放
短句来源
     THE CHROMOSOMES OF BOVINE LEUKEMIA
     白血病牛姊妹染色单体交换(SCE)与染色体畸变的观察
短句来源
     Study on Bovine Endometritis
     黄牛子宫内膜炎的研究
短句来源
     STUDIES ON VIRAL ARTHRITIS
     病毒性关节炎的研究
短句来源
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  bovine viral
Cloning and sequence analysis of genetic variation on NS2-3 of bovine viral diarrhea virus (HB-DCZ) strain in Hebei Province, Ch
      
The objective of this research is to analyze the genetic characterization of a bovine viral diarrhea virus (BVDV) strain (HB-DCZ strain) isolated from China and describe its relationship with other BVDV strains.
      
The correlation between microscopic changes with cellular localization of viral antigens was studied in the ileum of 16 cases infected with bovine viral diarrhea virus (BVDV).
      
Ten lots of specially procured fetal calf serum collected under sterile conditions and not filtered and 16 lots of commercial fetal calf serum were tested for both human and bovine viral contamination.
      
Approach for Qualitative Validation Using Aggregated Data for a Stochastic Simulation Model of the Spread of the Bovine Viral-Di
      
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A strain of bovine viral diarrhea virus (BVDV) was isolated from epidemic area of lamb in Inner Mongol in recent years During 1983-1985, we have deteted 229 shares of the fecal samples suffering from this illness in Inner Mongol, the shares of the viral samples account for 59 pereent.These virions appeared spherical and measured 40-80nm in diameter. Their surfaces have envelope structures. The virus can infect the lambs.22 passages virus have been acquired. The results of the complementary fixation...

A strain of bovine viral diarrhea virus (BVDV) was isolated from epidemic area of lamb in Inner Mongol in recent years During 1983-1985, we have deteted 229 shares of the fecal samples suffering from this illness in Inner Mongol, the shares of the viral samples account for 59 pereent.These virions appeared spherical and measured 40-80nm in diameter. Their surfaces have envelope structures. The virus can infect the lambs.22 passages virus have been acquired. The results of the complementary fixation test of virus showed that its titre have increased 6 times. The examine of pathoanatomy of the lambs infected by the virus showed that viral enteritis symptoms appeared in the lambs. The lamb's serum in recovery of its disease is positive by using serological assay.As the results described above, we preliminarily consider that this virus is a new strain of BVDV. called the InL strain of bovine viral diarrhea virus.

近年来,我们在内蒙羔羊下痢病流行区采集到一株牛病毒性腹泻病毒。83年到85年间,对内蒙三盟,二市,六旗(县),二个良种场的229份病粪样做了病毒检测,其阳性率达59%。该病毒粒子为直径40—80nm,具有囊膜的球形颗料。且与BVDV(牛病毒性腹泻病毒)的NADL株及Danish株有共同抗源。该病毒感染羔羊已传至22代,经补体结合反应测定,其效价提高了6倍。病羔羊的解剖病变观察,说明有病毒性肠炎的病变。病羊的恢复期血清测定为阳性。根据上述结果,我们初步鉴定该病毒为BVDV的一个株,暂命为BVDV的1nL株。

Nine hybridoma cell lines secreting monoclonal antibody(McAb) against lapinized Chinese strain of swine fever virus (SFV-C)were established by means of the fusion between mouse myeloma SP2/0cell and spleen cell from BALB/C mouse which were immunized withSFV-C antigen partly purified by PEG precipitation. The McAbsreacted only with SFV-C strain, but did not with SFV Shimen strainand bovine viral diarrhoea virus Oregon strain. All the McAbs arespecific for different antigen determinants, respectively, and...

Nine hybridoma cell lines secreting monoclonal antibody(McAb) against lapinized Chinese strain of swine fever virus (SFV-C)were established by means of the fusion between mouse myeloma SP2/0cell and spleen cell from BALB/C mouse which were immunized withSFV-C antigen partly purified by PEG precipitation. The McAbsreacted only with SFV-C strain, but did not with SFV Shimen strainand bovine viral diarrhoea virus Oregon strain. All the McAbs arespecific for different antigen determinants, respectively, and haveno precipitation character.

以聚乙二醇(PEG,MW6000)沉淀法部分纯化的猪瘟兔化弱毒中国株(SFV-C)免疫BALB/C小鼠,取其脾脏制备脾细胞与SP2/0骨髓瘤细胞融合,经ELISA检测和有限稀释法克隆化筛选出9株对SFV特异的单克隆抗体(McAb)杂交瘤细胞。它们产生的McAb仅对SFV-C株发生特异性反应,而与SFV-S及BV DV Oregon株不发生反应.相加试验表明,9个McAb分别针对不同的抗原决定簇。所有的McAb均不具有沉淀反应特性。

A bovine epidemical diarrhea of the yaks in Guoluo state is confirmed the diagnosis as the bovine viral diarrhea-mucosal disease by means of an epidemiological investigation and a seralogical diagnosis. A positive rate of BVD agar gel diffusion test of yak sera is from 3.17% to 14.81%.

对果洛州牦牛中流行的牛拉稀病,通过流行病学调查和血清学诊断确诊为牛病毒性腹泻/粘膜病。BVD血清琼脂扩散阳性率为3.17%—14.81%。

 
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