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cartilage cavities
相关语句
  软骨陷窝
     There are gap junctions in the cells in the same cartilage cavities in the articular cartilage of the human femoral head, while no gap junctions in the isolated chondrocytes in the cartilage.
     缝隙连接只出现在球形软骨细胞之间 ,与软骨细胞的特有表型同时出现 ; 股骨头人关节软骨陷窝内的软骨细胞间有缝隙连接存在 ,可以进行活体组织原位缝隙连接研究
短句来源
     histological results showed that cartilage cavities formed and that cartilage matrix was stained positively for toluidine blue .
     组织学切片上可见软骨陷窝形成 ,并分泌甲苯胺蓝异染的软骨基质。
短句来源
     In TE- C group, 12 weeks after operation, cartilage cavities and capsule were apparent in the defect and chondrocytes were in cell condensation.
     组织工程软骨组术后12周,缺损处可见软骨陷窝及软骨囊,软骨细胞成团状。
短句来源
  “cartilage cavities”译为未确定词的双语例句
     Objective To study the gap junction and the phenotype of the cultured chondrocyte of rabbit and the gap junction between the chonduocyres in the same cartilage cavities in human femoral head articular cartilage.
     目的 探讨体外培养状态下新生兔软骨细胞的缝隙连接(gap junction)与软骨细胞形态、表型的关系。
短句来源
     3, Record the fluorescent after blanching of chondrocyte in the cartilage cavities in the proliferative zone of articular cartilage of adult human femoral head.
     3.将因股骨颈骨折而行人工关节置换的人股骨头关节软骨切成20um薄片,同样加入荧光染料后测定淬灭前后荧光强度。
短句来源
     2, There are gap junctions in the cells in the same cartilage cavities in the articular cartilage of the human femoral head.
     2.缝隙连接只出现在球形软骨细胞之间,它与软骨细胞的特有表型同时出现。
短句来源
     Objective To study the gap junction and phenotype of cultured chondrocyte of rabbit ,and the gap junction between the chondrocytes in the same cartilage cavities in human femoral head articular cartilage.
     目的 探讨体外培养下新生兔软骨细胞的缝隙连接与软骨细胞形态、表型的关系。
短句来源
     And the fluorescent after blanching of chondrocyte in the cartilage cavities in the proliferative zone of articular cartilage of adult human femoral head was recorded, too.
     将因股骨颈骨折行人工关节置换的人股骨头关节软骨切成 2 0μm薄片 ,同样加入荧光染料后测定激光淬灭前后荧光强度。
短句来源
  相似匹配句对
     2, There are gap junctions in the cells in the same cartilage cavities in the articular cartilage of the human femoral head.
     2.缝隙连接只出现在球形软骨细胞之间,它与软骨细胞的特有表型同时出现。
短句来源
     histological results showed that cartilage cavities formed and that cartilage matrix was stained positively for toluidine blue .
     组织学切片上可见软骨陷窝形成 ,并分泌甲苯胺蓝异染的软骨基质。
短句来源
     ②Cartilage injury.
     ②软骨损伤检查结果。
短句来源
     On Casts With Complex Cavities
     复杂内腔铸件的反求建模
短句来源
     ③epiphyseal cartilage;
     ③骨骺软骨 ;
短句来源
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Objective To study the gap junction and the phenotype of the cultured chondrocyte of rabbit and the gap junction between the chonduocyres in the same cartilage cavities in human femoral head articular cartilage. Methods 1, Add CFDA - AM into the medium of the fifth generation of chondrocyte of rabbit in the 96 - well plate, and detect the fluorescent in spherical and fibroblast - like chongdrocytes separately. 2, Blanch the fluorescent with laser and record the recurrence of the fluorescent in accordant...

Objective To study the gap junction and the phenotype of the cultured chondrocyte of rabbit and the gap junction between the chonduocyres in the same cartilage cavities in human femoral head articular cartilage. Methods 1, Add CFDA - AM into the medium of the fifth generation of chondrocyte of rabbit in the 96 - well plate, and detect the fluorescent in spherical and fibroblast - like chongdrocytes separately. 2, Blanch the fluorescent with laser and record the recurrence of the fluorescent in accordant with time in 16 minutes. 3, Record the fluorescent after blanching of chondrocyte in the cartilage cavities in the proliferative zone of articular cartilage of adult human femoral head. Results The average fluorescent of the single layer of the fibroblast - like chondrocyte is 83 (range 1 -274 n = 30), the highest is found in the spherical shaped cell(average2057 range 340 - 3538 n = 30 ). 2, The recurrence of the fluorescent after the blanching is appeared only in the spherical chondrocyte, shown the gap junctions reappear only in the spherical chondrocytes, as will as in the cells in the cartilage cavities in the articular cartilage of the human femoral head. Conclusions 1, The appearance of the gap junction corresponding with the spherical shape of the chondrocyte. 2, There are gap junctions in the cells in the same cartilage cavities in the articular cartilage of the human femoral head.

目的 探讨体外培养状态下新生兔软骨细胞的缝隙连接(gap junction)与软骨细胞形态、表型的关系。方法 1.向形成集落的兔膝关节软骨细胞(第5代)培养基内加入荧光染料CFDA-AM,经激光共聚焦显微镜测量不同形态的单个软骨细胞内的荧光强度。2.以激光淬灭软骨细胞内的荧光后定时测量其荧光的恢复曲线。3.将因股骨颈骨折而行人工关节置换的人股骨头关节软骨切成20um薄片,同样加入荧光染料后测定淬灭前后荧光强度。结果 1.各种形态的扁平状软骨细胞摄入CFDA-AM后平均荧光强度差异很大(范围1-274,n=30),平均值明显低于单个球形软骨细胞者(平均2057,范围340-3538,n=30)。2.只有集落中央的球形软骨细胞才有逐渐的荧光恢复。3.人股骨头关节软骨陷窝内软骨细胞内的荧光经激光淬灭后可以恢复。结论 1.软骨细胞的形态影响其荧光染料的摄入量。2.缝隙连接只出现在球形软骨细胞之间,它与软骨细胞的特有表型同时出现。

Objective To study the gap junction and phenotype of cultured chondrocyte of rabbit ,and the gap junction between the chondrocytes in the same cartilage cavities in human femoral head articular cartilage. Methods CFDA AM was added into the medium of the fifth passage of chondrocyte of rabbit in the 96 well plate. The fluorescent in spherical and fibroblast like chondrocytes was detected separately. The recurrence of the fluorescent in accordant with time in 16 minutes was recorded after blanching...

Objective To study the gap junction and phenotype of cultured chondrocyte of rabbit ,and the gap junction between the chondrocytes in the same cartilage cavities in human femoral head articular cartilage. Methods CFDA AM was added into the medium of the fifth passage of chondrocyte of rabbit in the 96 well plate. The fluorescent in spherical and fibroblast like chondrocytes was detected separately. The recurrence of the fluorescent in accordant with time in 16 minutes was recorded after blanching the fluorescent with laser. And the fluorescent after blanching of chondrocyte in the cartilage cavities in the proliferative zone of articular cartilage of adult human femoral head was recorded, too. Results The average fluorescent of the single layer of the fibroblast like chondrocyte was 83(ranged from 1 to 274), the highest was found in the spherical shaped cell (averaged 2 057, ranged from 340 to 3 538). The recurrence of the fluorescent after the blanching appeared only in the spherical chondrocyte, the gap junctions reappeared only in the spherical chondrocytes, as well as in the cells in the cartilage cavities in the articular cartilage of the human femoral head. Conclusion The appearance of the gap junction is corresponded with the spherical shape, secretion of the cartilage matrix of the chondrocyte. There are gap junctions in the cells in the same cartilage cavities in the articular cartilage of the human femoral head, while no gap junctions in the isolated chondrocytes in the cartilage.

目的 探讨体外培养下新生兔软骨细胞的缝隙连接与软骨细胞形态、表型的关系。方法 向 96孔板内的第 5代新生兔膝关节软骨细胞培养基内加入荧光染料 CFDA- AM,激光共聚焦显微镜测量不同形态的单个软骨细胞内的荧光强度 ;以激光淬灭软骨细胞内的荧光后定时测量荧光恢复曲线。将因股骨颈骨折行人工关节置换的人股骨头关节软骨切成 2 0μm薄片 ,同样加入荧光染料后测定激光淬灭前后荧光强度。结果 各种形态的扁平状软骨细胞摄入CFDA- AM后平均荧光强度差异很大平均 83(1~ 2 74 ) ,平均值明显低于单个球形软骨细胞者平均 2 0 5 7(340~ 35 38) ;只有集落中央的球形软骨细胞才有逐渐的荧光恢复 ;但关节软骨陷窝内软骨细胞内的荧光经激光淬灭后可以恢复。结论 软骨细胞的形态影响其荧光染料的摄入量 ;缝隙连接只出现在球形软骨细胞之间 ,与软骨细胞的特有表型同时出现 ;股骨头人关节软骨陷窝内的软骨细胞间有缝隙连接存在 ,可以进行活体组织原位缝隙连接研究

Objective To investigate the feasibility of repairing the whole layer defects of tibial plateau by implanting tissue-engineering cartilage. Methods The chondrocytes of 2-week-old rabbits were cultured and transferred to the 3rd generation, and mixed with human placenta collagenl-sponge. The whole layer defects of tibial plateau in adult rabbits were repaired by the tissue-engineering cartilage in the experimental group; the defects were left un-repaired in control group. The repair results of defects were observed...

Objective To investigate the feasibility of repairing the whole layer defects of tibial plateau by implanting tissue-engineering cartilage. Methods The chondrocytes of 2-week-old rabbits were cultured and transferred to the 3rd generation, and mixed with human placenta collagenl-sponge. The whole layer defects of tibial plateau in adult rabbits were repaired by the tissue-engineering cartilage in the experimental group; the defects were left un-repaired in control group. The repair results of defects were observed after 4, 12 and 24 weeks. Results In experimental group, no obvious new cartilage formation was seen 4 weeks after operation; some new cartilage formation was found after 12 weeks. Histological observation showed that chondrocytes had irregular edge, honeycombing structure and that cartilage cavities formed around the chondrocytes. After 24 weeks, obvious new cartilage formation was found with smooth surface, and linked with the tissues around it, but the defect was not repaired completely; histological results showed that cartilage cavities formed and that cartilage matrix was stained positively for toluidine blue . In control group, the defect was not repaired. Conclusion The tissue-engineering cartilage can repair the defects of the whole layer cartilage of tibial plateau in rabbits, it is feasible to repair the whole layer cartilage defects of tibial plateau by this method.

目的 探讨运用组织工程软骨植入修复兔胫骨平台外侧髁全关节软骨浅层缺损的可行性。 方法 取 2周龄乳兔软骨细胞体外培养传代至第 3代后 ,与人胎盘 型胶原蛋白海绵复合后植入成年兔的胫骨体平台外侧髁浅层完全软骨缺损区 ,并设立空白对照组 ,分别于术后 4、12和 2 4周取材 ,观察修复效果。 结果 实验组术后 4周缺损表面未见明显的新生软骨形成 ;12周 ,缺损表面有少量的新生软骨形成 ,组织学切片上可见软骨细胞呈边缘不规则的 ,小蜂窝状结构 ,软骨细胞周围有软骨陷窝形成 ;2 4周 ,缺损表面的新生软骨较 4、12周的新生软骨明显 ,表面光滑 ,且与周围组织结合紧密 ,但仍存在部分缺损尚未修复。组织学切片上可见软骨陷窝形成 ,并分泌甲苯胺蓝异染的软骨基质。而对照组则均未见明显的修复。 结论 制成的组织工程软骨对兔胫骨平台软骨浅层完全缺损有修复作用 ,但不能完全修复缺损。

 
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