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growing tip
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  生长锥
     It was same the developmental order of calyx, corolla and stamen of six species plants,their growing tip of floral buds formed in turn 5 calyx primordia, 5 corolla primordia and 5 staminate primordia.
     其中6种植物的花萼、花冠及雄蕊的发育过程基本相同,均由花芽生长锥顶端依次向心分化出5个花萼原基、5个花冠原基及5个雄蕊原基发育而成。
短句来源
     2. To the millet, the sensitive stage during which male fertility and sterility would be converted into each other was from the stage of growing tip lengthening to the stage of stamen and pistil differentiation.
     2.幼穗生长锥伸长期至雌雄蕊分化期为光敏核不育谷子育性转换敏感期。
短句来源
     It displayed the sum-temperature effect in theperiod from the emergence of seedling to the elongation of growing tip andthe low temperature effect in the period from the elongation of growing tipto the appearance of spikelet primordium.
     温度作为春化阶段的必要条件,从出苗到生长锥伸长表现为积温效应,从生长锥伸长到小穗原基出现表现为低温效应。
短句来源
     The differentiation process of inflorescence was divided to 5 period: growing tip extention, flower peduncle differentiation, floret differentiation, primary flower flour appea-rence and ovary forming and flower flour maturity etc.
     将蓖麻花序分化过程划分为生长锥伸长膨大、花梗分化、小花分化、花粉粒和子房形成、花粉粒充实完成等五个时期。
短句来源
     Olny was the calyx development of Lycium barbarum L. special, at first, the outer cycle of the growing tip of floral bud formed cyclocalyx primordia, then growed and formed calyx with 2 lobes;
     唯宁夏枸杞的花萼发育特殊,由花芽生长锥顶端外周分化出一个环状花萼原基发育而成具2裂片的花萼;
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  “growing tip”译为未确定词的双语例句
     Growing tip of allooctoploid Triticale‘h739’plants and its pollen plants at the glume dif- ferentiation stage were cultured on MS medium supplemented with 2 mg/l 2,4-D. The callus began to be formed after 20 days.
     取异源八倍体小黑麦“h739”的植株和花粉植株护颖分化期的幼穗,培养在附加2mg/l2,4-D 的 MS 培养基上,接种后20天形成愈伤组织。
短句来源
     The results showed that the effect is the best for using growing tip in bud as explant to induce callus when callus is cultured through induction but the optimal induction medium was MS+1. 15 mg/L 6—BA+2.5% sucrose.
     MS+1.15 mg/L 6-BA+2.5%蔗糖为最佳诱导培养基。
短句来源
     Tissue Culture of the Growing Tip of Lilium hybridum SOLAIA
     索拉亚百合茎尖组织培养研究
短句来源
     2. The breadth of growing tip was positively relative to the sugarcane stem.
     2)甘蔗生长锥原生分生组织具有明显的细胞—组织学区域
短句来源
     The proced- ure and corresponding conditions obtained in the study were as follows: the shoot-tips were precultured on MS medium with 0.5M sucrose for 2 days and detached growing tip tissue with the size of 2~3mm was dehyrated with a vitrification solution (100%PVS2 Solu tion) for 20 minutes at 0℃. After new vitrification solution was replaced. Then plunge into liquid nitrogen.
     本研究得出百合茎尖超低温保存的程序和基本条件为:切取百合茎尖2~3mm,在0.5mol·L-1蔗糖的MS培养基上预培养1~2d后,0℃下用100%PVS2脱水处理20min后,换用新鲜的100%PVS2,然后将材料直接投入液氮。
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  相似匹配句对
     ELASTIC-PLASTIC FIELDS NEAR THE GROWING CRACK TIP
     扩展裂纹尖端弹塑性场
短句来源
     There are 2 lagers of mantle cells at the tip of growing point.
     在生长锥顶端有二层外套细胞。
短句来源
     Growing with China
     和中国一起成长
短句来源
     Growing Sculpture
     生长的雕塑
短句来源
     Tip story
     小费的故事
短句来源
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  growing tip
It was found that the stacking-fault substructure exists just in the growing tip of fresh bainitic plate and so does the shear stress field in the matrix around the tip.
      
The growth of twinned crystals of aluminium on solidification is discussed with reference to the structure of the growing tip.
      
Thus growth rate may be limited by food rather than by there being only one growing tip of the hydrorhizae.
      
When colchicine was applied directly to the apical growing tip of cotyledon-stage seedlings, leaf and stem growth was temporarily affected but the plants eventually recovered.
      
Cylindrical growth of fungal hyphae requires spatial organization of secretion to the growing tip.
      
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Material of Fokienia hodginsii was collected in 1964 from Fengyangshan (alt.1000—1400 M)in Lungchuan county,Chekiang province. This paper deals with the fertilization in Fokienia.It includs the structure of male and female gametes as wall as the process of fusion of their nuclei and cytoplasm re- spectively. The division of the spermatogenous cell of Fokienia occurred by the end of June (1964)and two sperms similar in shape and size were formed when pollen tube rea- ched the top of archegonia.Two equalsperms...

Material of Fokienia hodginsii was collected in 1964 from Fengyangshan (alt.1000—1400 M)in Lungchuan county,Chekiang province. This paper deals with the fertilization in Fokienia.It includs the structure of male and female gametes as wall as the process of fusion of their nuclei and cytoplasm re- spectively. The division of the spermatogenous cell of Fokienia occurred by the end of June (1964)and two sperms similar in shape and size were formed when pollen tube rea- ched the top of archegonia.Two equalsperms look like two hemispherical bodies conjoin- ed togather.The sperm possesses cell wall and is about 65 μ in diameter.Its nucleus is rather large and about 45—50 μ in diameter.There is a nucleolus in the nucleus. Outside the nucleus the dense cytoplasm forms the deep colored zone,some 10 μ in thickness.This zone is separated from the nucleus by a narrow perinuclear zone,and from the plasmalemma by a marginal zone.The perinuelear zone is about 2 μ thick, and the mariginal zone is from 3 to 4 μ thick.Both zones have transparent cytoplasm. When the archegonium is formed,the central cell has a small nucleus which is located below the neck cells.At the middle of June(1964),the central cell divides to form the ventral nucleus and the egg nucleus.The egg nucleus sites primarily at the upper part of archegonium and has only one nucleolus.Then the egg nucleus in- creases gradually in size and moves to the central part of the archegonium.In ma- ture archegonium there are usually 4—5,rarely 6—7 nucleoli in the egg nucleus,each of them is about 15 μ in diameter.The egg cell in Fokienia hodginsii is about 500 μ in length.The female nucleus is larger than the male one.After egg cell matures, its cytoplasm increases gradually,while the central vacuole decreases gradually and almost disappears completely after fertilization.It is interesting to note that there are 1—2 dense cytoplasm masses at the upper or lower part of egg nucleus.The shape of the mass is similar to that of the egg nucleus but no membrane is formed.These cy- toplasm masses are about 50—70 μ in dianmter in some cases. The fertilization of Fokienia took place at the end of June when the growing tip of pollen tube had reached the top of the archegonium.Then the neck cells become disorganized and degenerated.It is possible that all the cytoplasmic contents of pollen tubes are released into the archegonium.Before fertilization,the cytoplasm around the sperms and sterile cell and tube nucleus are in front of these two sperms.Then the sperms separate from each other and come down into the cytoplasm of the egg. When the male nucleus contacts with the egg nucleus,both become flattened along their contaet surface.Then the nuclear membranes of both sperm and egg nuclei be- eome ultimately disintegrated.Thus the fusion process is complete.However,it is usually seen that the entire sperm nucleus does not sink into the egg nucleus in Fokie- nia,though the opposite is the case in an exceptional example.When the sperm nuc- leus passes into the cytoplasm of egg cell,its cytopasm is released inside the arche- gonium along with it.During the course of fusion of the male and female nuclei,the fertilized nucleus is surrounded by both female and male cytoplasm.Thus the male cytoplasm along with the peripheral cytoplasm of the egg cell invests the two nuclei lying in contact and forms a dense neocytoplasm.When the zygote divides,the neocy- toplasm is full of the starch grains and a dense cytoplasm sheath is formed. After fertilization,the fused nucleus moves toward the base of the egg cell.It seems that the movement of the fused nucleus is not a simple mechanical movement but turned over repeatedly toward the base of the archegonium.Sometimes the position of the sperm and egg nuclei makes a turn of 180°.At the same time the track of the fertilized egg nucleus with vacuoles in the archegonium may be traced. After zygote moves into basal part of the archegonium,first intranuclear mitosis occurs.The nuclear envelop of zygote disappears gradually at the telophase of the first mitosis.Then division of the free nuclei of proembryo follows. From fertilization to the stage of proembryo formation,the second sperm may sometines enter into the cytoplasm of the egg cell.Mitosis of the second sperm nucleus may take place(fig.17—18)in the upper part of the archegonium.In addition,there are often several supernumerary nuclei(as many as 7—8 in number)in the same egg cell.These nuclei are also surrounded by dense cytoplasm.They may persist for some time and be recognizable at somewhat later stages of the proembryo or even after the elongated suspensors are formed.In some cases,there are some cell groups above the upper tier of proembryo.These cell groups are also surrounded by dense cytoplasm. Either the supernumerary nuclei or cells are surrounded by the dense cytoplasm.Pro- bably they are derived from the mitosis or amitosis of the second sperm. Investigations on submicroscopic structures of sperm and egg in relation to the fer- tilization of Cupressaceae have been carried out extensively during the last decade.The fate of male cytoplasm has been debated for a long time and this problem attracted attention again in the nineteen seventies.At last the concept of neocytoplasm has been established soundly based upon the information from observation of electron microp- hotographs.The neocytoplasm is also visible under the light microscope though the components are not recognizable.The sperms of Fokienia are similar to those of Cupressus funebris,Juniperus communis,Sabina virginiana,Tetraclinis articulata,Cha- maecyparis pisifera as well as the genus Thujopsis and others.Two sperms are all effective in fertilization and this is the common phenomenon of the family Cupres- saceae.

福建柏精原细胞分裂后,产生两个形态和大小都相同的雄配子——精子;精子形成时,犹如两个相连的半球体,各具细胞壁。中央细胞分裂产生腹沟核和卵核,腹沟核形成后逐渐退化;卵核却迅速增大。6月下旬,精核在颈卵器中部或中上部与卵核相遇,进行受精作用。在精卵融合过程中,进入卵细胞的雄细胞质,逐渐包围受精卵,并与部分卵细胞质结合形成新细胞质。受精后,在新细胞质中,又呈现大量淀粉粒。当受精卵移到颈卵器底部时,合子开始进行第一次有丝分裂。在原胚和幼胚发育阶段,多余精核还可进入卵细胞,并进行有丝分裂,以致形成7—8个游离核。

Potato plants of four varieties growing in field and showing symptoms of epinasty and stunt were tested for the presence of PSTV by polyacrylamide gel electrophoresis according to Morris and Smith (1977). More than half of such plants have given PSTV-RNA bands in polyacrylamide gels. These positive results have been improved by tomato bioassay and infectivity test of PSTV-RNA eluted from the gels. The test of quantities of terminal growing tips showed, 2—4g of infected tissue often gives more stable...

Potato plants of four varieties growing in field and showing symptoms of epinasty and stunt were tested for the presence of PSTV by polyacrylamide gel electrophoresis according to Morris and Smith (1977). More than half of such plants have given PSTV-RNA bands in polyacrylamide gels. These positive results have been improved by tomato bioassay and infectivity test of PSTV-RNA eluted from the gels. The test of quantities of terminal growing tips showed, 2—4g of infected tissue often gives more stable results. Diagnosis of PSTV by acrylamide gel electrophoresis is more repid and reliable than the tomato bioassay for certification of disease-free seed potatoes and eliminating this disease from seed breeding lines.

用Morris和Smith(1977)的聚丙烯酰胺凝胶电泳方法,对五个马铃薯品种“冰岛”3号,“燕子”,“米拉”“克疫”和国外引种Y—18等45株地上部分出现“束顶”、矮缩、上部叶片直立、卷曲的田间感病植株进行鉴定。在其中半数以上的植株中发现了PSTV。取电泳测定阳性的病株茎尖组织汁液摩擦接种Rutgers番茄,进行生物测定。证明和电泳测定结果基本一致。为证实凝胶电泳分离的PSTV—RNA的侵染性,从凝胶柱的PSTV—RNA区带中提取核酸,感染Rutgers番茄,结果表明侵染性和PSTV—RNA区带是一致的。每次鉴定所需病株茎尖组织样品量的试验表明,取2—4克组织进行凝胶电泳,测定的重复性较好。实验证明用聚丙烯酰胺凝胶电泳鉴定马铃薯田间植株中的PSTV,迅速、可靠、两天内可完成12个样品的鉴定,适于在无病毒种薯生产、抗病毒育种及无病亲本选育中常规应用。

The discovery of the nerve growth factor(NGF),a specific protein material,by Rita Levi-Montalcini and her colleagues(1954)led to the observation that ifNGF is added to the culture medium,sympathetic and sensory neurons grow well inculture,for it is a potent and specific maintenance factor for these two types of neu-rons.Therefore,we chose the superior cervical ganglion(SCG)as a model systemfor nerve tissue culture in order to study the development,differentiation and rege-neration of nervous tissue in vitro.This...

The discovery of the nerve growth factor(NGF),a specific protein material,by Rita Levi-Montalcini and her colleagues(1954)led to the observation that ifNGF is added to the culture medium,sympathetic and sensory neurons grow well inculture,for it is a potent and specific maintenance factor for these two types of neu-rons.Therefore,we chose the superior cervical ganglion(SCG)as a model systemfor nerve tissue culture in order to study the development,differentiation and rege-neration of nervous tissue in vitro.This report presents what we have observed onthe cultures of SCG that have been successfully established in our laboratory bymeans of explant technique.SCG from newborn rats were explanted onto collagen or plasma-coated coverslipsand maintained in Maximow depression slide assemblies at 37℃.The fluid me-dium was replaced twice a week and consisted of 1/3 calf serum,1/3 synthetic medium199 and 1/3 Hanks' BSS,or of equal parts of calf serum and Hanks' BSS,supplementedwith 600 mg% glucose.NGF(a crude extract of mouse salivary gland)was added tothe medium in a proportion of 1:20;for control,no NGF was added to the medium in some cultures.The cultures were examined microscopically every day,Nissl's stai-ned,Bodian's protargol and ammonium silver impregnated preparations were madeperiodically.Time lapse microcinematographic records were also made.We have planted altogether 209 SCG from newborn rats.All of them grew suc-cessfully in culture with NGF or without NGF,but the SCG with NGF grew muchmore rapidly than those without NGF.NGF strongly stimulates the outgrowth ofneurites and maintains the survival of the sympathetic neurons.Neurites grew outindividually or,more frequently,as bundles extending radially toward the peripheryor forming a meshwork.The tips of elongating neurites expanded to form growthcones from which are projected long slender microspikes(filopodia).These microspi-kes continually waved about,extending,and retracting as the growth cone movedover a substratum.The behavior of the growing tip of neurite is best analyzed bytime lapse microcinematography.The Schwann cells emerged from the explant andproliferate to accompany the neurites.These cells,fusiform or filiform in shape,were usually arranged in alignment along the neurites.They could be easily distingui-shed from fihroblasts.Mitosis of Schwann cells had been observed and recorded bytime lapse microcinematography.The bodies of the sympathetic neurons did not mig-rate away from the explant.

应用 Maximow 双盖片法培养新生大白鼠颈上节植块。一部分培养物的培养液添加神经生长因子(NGF)粗制剂,另一部分未用 NGF,两者均获得生长。但应用 NGF 的培养物比不用 NGF 的培养物生长快速。NGF 对神经突起的生长和维持神经元的生存有极显著的促进作用。神经突起可单条或集合成束呈辐射状生长,或交织成网,或象树枝样向四周扩展。神经突起末端的生长锥是生长最活跃的部分。雪旺细胞呈梭形或线形,与成纤维细胞容易鉴别,它们常贴附神经突起生长。本研究并藉相差显微缩时电影摄影,记录了神经突起及其末端生长锥的生长活动状况和雪旺细胞的有丝分裂过程。交感神经元的胞体停留在植块内,未见外移。

 
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