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Differential extraction of axonally transported proteoglycans


Two RNA fractions, one enriched in rRNA and the other in "polydisperse" RNA, were obtained by differential extraction with phenol.


Similar responses were elicited by the hexane and chloroformmethanol fractions after differential extraction of boll weevil feces.


We also used a differential extraction technique to identify the major cuticular proteins of the adults.


We also used a differential extraction technique to identify the major cuticular proteins of the adults.

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 Objective\ To study the relationship between intracellular actin and scar contracture. Methods\ Fibroblasts from 10 cases of hypertrophic scar and 5 cases of keloid were cultured in vitro . Total actin, filamentous actin(F actin), globular actin (G actin) and the ratio of F to G actin(F/G) were measured by densitometry after differential extraction and seperation by polyacrylamide gel electrophoresis in the presence of sodium sulfate. Results\ Total actin, F actin, G actin and F/G in hypertrophic scar... Objective\ To study the relationship between intracellular actin and scar contracture. Methods\ Fibroblasts from 10 cases of hypertrophic scar and 5 cases of keloid were cultured in vitro . Total actin, filamentous actin(F actin), globular actin (G actin) and the ratio of F to G actin(F/G) were measured by densitometry after differential extraction and seperation by polyacrylamide gel electrophoresis in the presence of sodium sulfate. Results\ Total actin, F actin, G actin and F/G in hypertrophic scar fibroblasts were 2.38 ng/10 4 cells, 0.98 ng/10 4 cells, 1.42 ng/10 4 cells and 0.68 respectively, while in keloid fibroblasts were 1.68 ng/10 4 cells. 0.46 ng/10 4 cells, 1.26 ng/10 4 cells, and 0.36 respectively. There was significant differences between two tissues fibroblasts in the items of total actin, F actin, G actin, and F/G ( P<0.01) , while no significant difference in G actin( P>0.05) . Conclusion\ Total intracellular actin, F actin, and F/G may play an important role in the scar contracture. The hypertrophic scar and keloid can be distinguished by the contents of total intracellular actin, F actin and F/G.  目的 探讨增生性瘢痕、瘢痕疙瘩成纤维细胞中肌动蛋白 (actin)与瘢痕挛缩的关系。方法 取增生性瘢痕 10例 ,瘢痕疙瘩 5例 ,对成纤维细胞进行培养 ,并抽提细胞内蛋白成份后用 SDS PAGE电泳 ,然后通过凝胶扫描仪测定两种组织来源的成纤维细胞内的总肌动蛋白、纤维状肌动蛋白 (F肌动蛋白 )、球形肌动蛋白 (G肌动蛋白 )的量 ,并计算 F/ G肌动蛋白的比值。结果 增生性瘢痕细胞每 10 4个细胞内含总肌动蛋白、F肌动蛋白、G肌动蛋白分别为 2 .38ng、0 .98ng、1.42 ng,F/ G肌动蛋白的比值为 0 .6 8,而瘢痕疙瘩分别为 1.6 8ng、0 .46 ng、1.2 6 ng及 0 .36。统计学分析 ,在总肌动蛋白、F肌动蛋白及 F/ G肌动蛋白的比值上两种瘢痕有非常显著性差异(P<0 .0 1) ,而 G肌动蛋白在两种瘢痕中无显著性差异 (P>0 .0 5 )。结论 瘢痕挛缩与细胞内总肌动蛋白、F肌动蛋白及 F/ G肌动蛋白的值密切相关 ,细胞内总肌动蛋白、F肌动蛋白及 F/ G肌动蛋白的比值不同可作为区分增生性瘢痕和瘢痕疙瘩的依据之一。  Objective To investigate the different expression of intracellular actin and calcium in the fibroblasts from hypertrophic scars and keloid.Methods Ten cases of either hypertrophic scar or keloid were chosen randomly.The total intracellular actin,filamentous actin (F actin),globular actin (G actin) and the ratio of F/G actin were determined by densitometry after differential extraction and seperation by polyacrylamide gel electrophoresis.Fura 2/AM was applied as fluorescent Ca 2+ indicator to determine... Objective To investigate the different expression of intracellular actin and calcium in the fibroblasts from hypertrophic scars and keloid.Methods Ten cases of either hypertrophic scar or keloid were chosen randomly.The total intracellular actin,filamentous actin (F actin),globular actin (G actin) and the ratio of F/G actin were determined by densitometry after differential extraction and seperation by polyacrylamide gel electrophoresis.Fura 2/AM was applied as fluorescent Ca 2+ indicator to determine the intracellular concentration of free Ca 2+ for imaging analysis.Results In the fibroblasts from hypertrophic scars,the content of intracellular actin,the F actin,the ratio of F/G actin and intracellular calcium (2.38±0.35)?ng/10 4 cells,(0.98±0.19)?ng/10 4 cells,(0.68±0.07) and (82.64±10.00) respectively was much higher than those (1.68±0.25)?ng/10 4 cells,(0.46±0.10)?ng/10 4 cells, (0.36±0.05) ,(73.26±2.55) respectively in keloid (P<0.01).There was no significant difference in the G actin contents (P>0.05) between these two kinds of scar.Conclusion The content and polymerization of intracellular actin combined with the Ca 2+ played an important role in the scar contracture.  目的 探讨增生性瘢痕、瘢痕疙瘩成纤维细胞中肌动蛋白 (actin)和钙离子 (Ca2 + )含量与瘢痕挛缩的关系。方法 取两类瘢痕各 10例 ,蛋白质电泳及凝胶扫描测定成纤维细胞内总肌动蛋白 ,聚合 (F肌动蛋白 )、球 (G肌动蛋白 )肌动蛋白及F/G肌动蛋白比值。Fura 2荧光探剂标记、图像分析胞内Ca2 + 浓度。结果 增生性瘢痕总肌动蛋白 [(2 .3 8± 0 .3 5 )ng/10 4细胞 ]、F肌动蛋白 [(0 .98± 0 .19)ng/10 4细胞 ]、F/G肌动蛋白比值 (0 .68± 0 .0 7)、Ca2 + 浓度 (82 .64± 10 .0 0 )均明显高于瘢痕疙瘩总肌动蛋白 [(1.68± 0 .2 5 )ng/10 4细胞 ]、F肌动蛋白 [(0 .46± 0 .10 )ng/10 4细胞 ]、F/G肌动蛋白比值 (0 .3 6± 0 .0 5 )及Ca2 + 浓度 (73 .2 6± 2 .5 5 ) (P <0 .0 1)。G 肌动蛋白含量差异无显著性 (P >0 .0 5 )。结论 成纤维细胞内肌动蛋白与钙离子浓度的改变 ,是造成两类瘢痕不同挛缩特性的重要原因。  The extraction of oil from soybean with supercritical CO 2 was studied in a continuousflow extraction apparatus at a pressure of 2030MPa and a temperature of 308323K.The results showed that the oil in soybean could be extracted out completely in this way. A mathematical model based on the hypothesis of fixed bed,plugflow in integral run and mixedflow in differential run is set up.Using this model we can calculate the results of integral extraction on the base of differential extraction which uses... The extraction of oil from soybean with supercritical CO 2 was studied in a continuousflow extraction apparatus at a pressure of 2030MPa and a temperature of 308323K.The results showed that the oil in soybean could be extracted out completely in this way. A mathematical model based on the hypothesis of fixed bed,plugflow in integral run and mixedflow in differential run is set up.Using this model we can calculate the results of integral extraction on the base of differential extraction which uses small materiel and occupies less time.The solving method of this model is simple.Under the condition in this paper,the deviation is less than 6%.  本文建立了一套超临界流体萃取的实验装置 ,在压力为 2 0MPa～ 30MPa ,温度为30 8K～ 32 3K的条件下 ,研究了用超临界二氧化碳萃取大豆油。试验证明用超临界流萃取的方法可以较为完全地得到大豆中的油分。基于固定床、积分柱塞流与微分混合流的假设建立了理论计算模型 ,使用这个模型可以根据装料量少耗时短的微分萃取试验结果来较为准确地计算出相同条件下积分萃取的过程。经本试验结果比较证明该方法简便可行 ,在本文条件下误差小于 6 % ,是对超临界流体萃取放大研究的一种探索  
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