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xinjiang melon
相关语句
  新疆甜瓜
    The attractive action of BTH on Xinjiang melon peroxidase
    BTH对新疆甜瓜过氧化物酶的系统诱导作用
短句来源
    A High Quality and Disease-resistant New Line of Xinjiang Melon Obtained by Agrobacterium Tumefacien-mediated Transformation
    通过根癌农杆菌介导法获得新疆甜瓜抗病优质新品系
短句来源
    Disease Resistance Transfering Breeding of Xinjiang Melon and its Effect
    新疆甜瓜的抗病性转育及其效果
短句来源
    Effect of BTH on Attractive Action of Xinjiang Melon's Plants Peroxidase and Its Isoenzymes
    BTH诱导处理对新疆甜瓜植株过氧化物酶的诱导作用及对其同工酶的影响
短句来源
    Chitin enzyme gene and β- 1,3- carbohydrase gene were transformed into XinJiang melon. Visuses infecting melons were detected with the aid of technology of enzyme linked im- munosorbent assay (ELISA) and immunosorbent electron microsc (ISEM).
    将几丁质酶基因和β-1,3-葡聚糖酶基因导入新疆甜瓜,并借助于酶联免疫分析(enzymelinkedimmunosorbentassay,ELISA)、免疫电镜分析(immunosorbentelectronmicroscope,ISEM)技术从感病甜瓜检测病毒粒子;
短句来源
  新疆甜瓜
    The attractive action of BTH on Xinjiang melon peroxidase
    BTH对新疆甜瓜过氧化物酶的系统诱导作用
短句来源
    A High Quality and Disease-resistant New Line of Xinjiang Melon Obtained by Agrobacterium Tumefacien-mediated Transformation
    通过根癌农杆菌介导法获得新疆甜瓜抗病优质新品系
短句来源
    Disease Resistance Transfering Breeding of Xinjiang Melon and its Effect
    新疆甜瓜的抗病性转育及其效果
短句来源
    Effect of BTH on Attractive Action of Xinjiang Melon's Plants Peroxidase and Its Isoenzymes
    BTH诱导处理对新疆甜瓜植株过氧化物酶的诱导作用及对其同工酶的影响
短句来源
    Chitin enzyme gene and β- 1,3- carbohydrase gene were transformed into XinJiang melon. Visuses infecting melons were detected with the aid of technology of enzyme linked im- munosorbent assay (ELISA) and immunosorbent electron microsc (ISEM).
    将几丁质酶基因和β-1,3-葡聚糖酶基因导入新疆甜瓜,并借助于酶联免疫分析(enzymelinkedimmunosorbentassay,ELISA)、免疫电镜分析(immunosorbentelectronmicroscope,ISEM)技术从感病甜瓜检测病毒粒子;
短句来源
  新疆甜瓜
    The attractive action of BTH on Xinjiang melon peroxidase
    BTH对新疆甜瓜过氧化物酶的系统诱导作用
短句来源
    A High Quality and Disease-resistant New Line of Xinjiang Melon Obtained by Agrobacterium Tumefacien-mediated Transformation
    通过根癌农杆菌介导法获得新疆甜瓜抗病优质新品系
短句来源
    Disease Resistance Transfering Breeding of Xinjiang Melon and its Effect
    新疆甜瓜的抗病性转育及其效果
短句来源
    Effect of BTH on Attractive Action of Xinjiang Melon's Plants Peroxidase and Its Isoenzymes
    BTH诱导处理对新疆甜瓜植株过氧化物酶的诱导作用及对其同工酶的影响
短句来源
    Chitin enzyme gene and β- 1,3- carbohydrase gene were transformed into XinJiang melon. Visuses infecting melons were detected with the aid of technology of enzyme linked im- munosorbent assay (ELISA) and immunosorbent electron microsc (ISEM).
    将几丁质酶基因和β-1,3-葡聚糖酶基因导入新疆甜瓜,并借助于酶联免疫分析(enzymelinkedimmunosorbentassay,ELISA)、免疫电镜分析(immunosorbentelectronmicroscope,ISEM)技术从感病甜瓜检测病毒粒子;
短句来源
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he cotyloden strips of six Xinjiang melon varieties (lines)" Xin Hongxincui" etc. were transformed with Cucumber Mosaic Virus Coat Protein (CMV - CP) gene by A. tumefacien-mediated method, and large amo unt of anti-kanamycin (kan) plant were obtainted. After test by Luis method,some anti-kan plants showed the activity of nopalin synthase. Further tests, such as: PCR, dot blot,western blot, etc,indicated that the CMV-CP gene had integrated in the genomic DNA of the anti-kan plants, and the expression of...

he cotyloden strips of six Xinjiang melon varieties (lines)" Xin Hongxincui" etc. were transformed with Cucumber Mosaic Virus Coat Protein (CMV - CP) gene by A. tumefacien-mediated method, and large amo unt of anti-kanamycin (kan) plant were obtainted. After test by Luis method,some anti-kan plants showed the activity of nopalin synthase. Further tests, such as: PCR, dot blot,western blot, etc,indicated that the CMV-CP gene had integrated in the genomic DNA of the anti-kan plants, and the expression of this 1 kb gene in plant produced a protein of 24 kDa, an expected size for CMV-CP. The expression amount was about 5% of total plant protein. After traditional breeding of 9generations, the new melon line K3 - 19 of high quality and disease-resistance of stable heridity was obtained from these anti-kan plants.

用新疆甜瓜无菌苗子叶块,通过根癌农杆菌介导法,将黄瓜花叶病毒外壳蛋白cDNA基因导入“新红心脆”等6个品种(系),经卡那霉素(Kanamycin,Km)筛选.获得大量Km抗性植株,其中部分Km抗性植株经Luis法检测,表达了胭脂碱合成酶活性。进一步经SDS-PAGE,DOT-Elisa,PCR特异扩增,Western-Blot等方法检测,结呆证明了Km抗性植株基因组中整合了CMV—CP基因,长度为1kb,并能有效表达.表达产物分子量为24kD,确为CMV的外壳蛋白,其表达量占细胞总蛋白的5%。Km抗性植株经常规育种选育9代,获得了稳定过传的抗病、优质甜瓜品系K3-19。

In order to select disease resistant Xinjiang melon breeding materials, two Xinjiang melon lines(highly susceptible to powdery and downy mildew) and one American cantaloupe melon variety (highly resistant to powdery and downy mildew) were took as the primary parents.Using the method of seedling inoculation screen and plant resistance field screen combined with fruit quality screen. Two Xinjiang melon lines which have high resistance to powdery and downy mildew were obtained after 1~2...

In order to select disease resistant Xinjiang melon breeding materials, two Xinjiang melon lines(highly susceptible to powdery and downy mildew) and one American cantaloupe melon variety (highly resistant to powdery and downy mildew) were took as the primary parents.Using the method of seedling inoculation screen and plant resistance field screen combined with fruit quality screen. Two Xinjiang melon lines which have high resistance to powdery and downy mildew were obtained after 1~2 backcrosses and 7~8 selfings in three experimental spots which differ greatly in ecological environment.

为选育新疆甜瓜类型抗病育种材料,以2个高感白粉病和霜霉病的新疆甜瓜材料和一个高抗白粉病和霜霉病的美国Cantaloupe类型甜瓜品种为原始亲本,分别进行杂交,采用苗期接种抗性鉴定,植株成株期田间自然抗性筛选结合果实品质鉴定的方法,在3个生态环境差异较大的试验点经1~2代回交和7~8代自交,转育出2个高抗白粉病和霜霉病的新疆甜瓜类型单系材料。

In this thesis, the attractive action of BTH on Xinjiang melon peroxidase was studied. Three concentrations of BTH are 25 μg·ml~(-1),50 μg·ml~(-1),100 μg·ml~(-1), which treated Early Golden Queen and Queen. The first and second leaf of melon were assayed by the peroxidase activity after the treatment with BTH. It was confirmed that perixidase activity has direct relation to melon' strain. At the same time, BTH can induce higher peroxidase activity and this conductivity can transmit from the...

In this thesis, the attractive action of BTH on Xinjiang melon peroxidase was studied. Three concentrations of BTH are 25 μg·ml~(-1),50 μg·ml~(-1),100 μg·ml~(-1), which treated Early Golden Queen and Queen. The first and second leaf of melon were assayed by the peroxidase activity after the treatment with BTH. It was confirmed that perixidase activity has direct relation to melon' strain. At the same time, BTH can induce higher peroxidase activity and this conductivity can transmit from the first leaf to the second leaf after 6 days of the first melon enzyme perk on the Queen, which was affected by significantly, and the best concentration is (25 μg·ml~(-1).)At the same time, the same result was got on the Early Golden Queen, but this effect was not significant. But the time of conductivity was different from different resistance cultivars. This experiment showed that the peroxidase activity in the tissue of melon which did not spray inducer was induced, the domino effect can be transferred by transfer tissue.

 采用3种浓度(25,50,100μg·ml-1)的BTH对耐病品种皇后和感病品种早金的第一片真叶进行诱导处理后,经诱导后不同时间测定第一片和第二片真叶的过氧化物酶(POD)活性,结果表明:BTH的三种不同的浓度处理均能诱导出耐病品种皇后第一片和第二片真叶的过氧化物酶(POD)活性的升高,与空白差异显著,以25μg·ml-1BTH处理的过氧化物酶(POD)活性最高,空白的为最低。第一片真叶的酶活性高峰为诱导处理后的第7天和第16天,第二片真叶的酶活性高峰为诱导处理后的第16天。BTH的三种不同的浓度处理也能诱导出感病品种早金第一片和第二片真叶的过氧化物酶(POD)活性的升高,但与空白差异不显著。以25μg·ml-1BTH处理的过氧化物酶(POD)活性最高,空白的最低。证明了甜瓜抗病性与甜瓜植株过氧化物酶活力呈正相关。BTH诱导酶活性的效应可以通过植物的输导组织,传导给其它未经处理的甜瓜组织,诱导其它组织中的过氧化物酶(POD)活性的升高。以25μg·ml-1BTH诱导效果最好。

 
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