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scar marker
相关语句
  scar标记
     Cloning of RAPD marker and studying of translating to SCAR marker on F gene of peach (Prunus persica(L.) Batsch)
     桃(Prunus persica (L.) Batsch) F基因RAPD标记的克隆及SCAR标记的转化研究
短句来源
     Conversion OPW03-900 of RAPD which linked to pollen sterility gene to SCW03-906 of SCAR marker.
     把与桃花粉育性基因连锁的RAPD标记OPW03-900转化为SCAR标记SCW03-906,该序列已被Genebank收录,登录号为DQ659676。
短句来源
     SCAR marker linked to seedless genes in grapes and southern blot analysis
     葡萄无核基因的SCAR标记及Southern blot分析
短句来源
     The E-ACG/M-CAG-180 marker had transferred into a co-dominante SCAR marker(SCAR3-109), and the genetic distances was 11 cM.
     将E-ACG/M-CAG-180特异片段转化成共显性的SCAR标记SCAR3-109,与ZYMV-CH的抗性基因遗传连锁距离为11cM。
短句来源
     Based on the sequence of the polymorphic DNA fragment, one specific primer pair was designed with the software of Primer Premier 5.0(SC1:5'-ACAAGTTGGAGTCAGGAG -3'; SC2:5'- ATCGTATGGTTTCGTCTT -3')- The AFLP marker was transformed to a sequence characterized amplified region(SCAR) marker, and all isolates were tested with the SCAR marker.
     根据该特异性DNA片段测序结果,用Primer Premier 5.0软件分析其序列,并设计出一对特定引物(SC_1:5′-ACAAGTTGGAGTCAGGAG-3′;SC_2:5′-ATCGTATGGTTTCGTCTT-3′),用此引物对可定性扩增出小麦光腥黑粉菌DNA特异性区段,将AFLP分子标记转化为SCAR标记
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  “scar marker”译为未确定词的双语例句
     They were ISSR-AW44123, ISSR-AW44116, ISSR-AW44117, ISSR-AW44133, ISSR-AW44137 and RAPD-C27932, RAPD-C27925.6^ We verified the result of SCAR marker, but only SCAR -ISSR-AW44123 reached over 70%and SCAR—RAPD-C27932 reached over 80%, oters was only about 20
     其中SCAR—ISSR—AW44123转换成功率达到70%,SCAR—RAPD—C27932转换成功率达到80%,从而为白桦长纤维标记辅助育种奠定了基础。
短句来源
     SCS13 L/SCS13R (SCAR marker of S13_2800) could be used to identify N and S cytoplasm, and its optimal annealing temperature was 59℃.
     其中SCS13L/SCS13R可成功地区分N、S两类胞质,适宜退火温度为59℃。
短句来源
     Application of SCAR Marker for Pm21 in Marker-assisted Selection for Powdery Mildew Resistance in Wheat
     小麦抗白粉病基因Pm21分子标记辅助选择的应用
短句来源
     The CBJ312 had amplification with template of the positive RAPD_S312 F2 individuals and opposite DNA pool, did not have amplification with the template of the negative RAPD_S312 F2 individuals and alternative DNA pool. So did the SCAR marker CBJ360.
     在对生池和F_2的RAPD_S360阳性单株中CBJ360的阳性率为100%,F_2的RAPD S360阴性单株没有检测到CBJ312扩增。
短句来源
     After the linkage analysis between these PCR markers and the resistance gene among F 2 population plants of HW642/Zhong8601, the results indicated that gwm37 -450 and SC W37 450 were closely linked to the BYDV resistance gene and the SCAR marker SC W37 450 was more reliable and easily scored.
     利用 HW6 4 2 /中 86 0 1的 F2 群体 ,对 gwm 37- 450 和 SC- W374 50 进行分析 ,结果表明 ,二者均与抗黄矮病基因紧密连锁 ,后者较前者稳定。
短句来源
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  相似匹配句对
     SCAR marker of Bph from rice
     水稻抗褐飞虱基因SCAR标记的获得
短句来源
     The Scar
     疤痕
短句来源
     Study on the SCAR Molecular Marker Research of Kava
     卡瓦胡椒SCAR标记的研究
短句来源
     Myofibroblast and scar
     肌成纤维细胞与瘢痕
短句来源
     CEO’S Marker
     CEO的标签
短句来源
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  scar marker
Polymorphism of one SCAR marker differed from that revealed with random primers.
      
The transition from complex RAPD spectra to amplification of a particular SCAR marker substantially facilitates analysis of large samples for the presence or absence of the examined fragment.
      
Molecular characterization of RAPD and SCAR marker linked to the frog-eye leaf spot resistance gene in soybean
      
A co-dominant SCAR marker SCS3620 >amp;amp; 580 has been developed based on the sequences.
      
The segregation of SCS3620 >amp;amp; 580 is similar to that of RAPD marker OPS03620 >amp;amp; 580-Significant polymorphism has been shown between resistant and susceptible genotypes when 62 soybean genotypes were surveyed for the SCAR marker.
      
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Seeds of mung bean Zhong Lü 1 were carried into space in 1994 by a recoverable satellite. After three years ground cultivation and selection, a long pod mutant line was obtained. The pod length of this mutant line ranged from 13 to 17cm and averaged 16cm. The seed setting number in each pod ranged from 15 to 19,averaged 17. By RAPD (Randomly Amplified Polymorphic DNA) analysis on this mutant line and its original CK line, 3 of total 100 10 mer Operon primers amplified polymorphic products were selected....

Seeds of mung bean Zhong Lü 1 were carried into space in 1994 by a recoverable satellite. After three years ground cultivation and selection, a long pod mutant line was obtained. The pod length of this mutant line ranged from 13 to 17cm and averaged 16cm. The seed setting number in each pod ranged from 15 to 19,averaged 17. By RAPD (Randomly Amplified Polymorphic DNA) analysis on this mutant line and its original CK line, 3 of total 100 10 mer Operon primers amplified polymorphic products were selected. The molecular weight of the specific amplified products was 1.4 kb, 1.0 kb, 2.0 kb respectively, therefore they were named as OPZ 13 1400 , OPY 07 1000 and OPY 04 2000 respectively. So far, the cloning of OPZ 13 1400 and OPY 07 1000 has completed. One of them, OPY 07 1000 was transferred further into stable SCAR marker. Three samples with a same phenotype and with about the same pod length selected from this mutant line showed no polymorphism in their RAPD pattern. But they differed from the original CK line with the same polymorphism. These results indicated that the long pod mutant line may be close to be stable in genetics.

中绿1号绿豆种子于1994年经返回式卫星搭载后,经过3年的地面种植和筛选得到了基本稳定的长荚型突变系,该突变系荚长16cm左右,每荚种子粒数为15~19粒。通过对3个形态和荚长都基本稳定的突变系和原始对照品系之间的RAPD分析,从100个10-merOperon引物中筛选出3个能产生稳定的遗传多态性的引物,其特异扩增产物分子量依次为1.4kb、1kb、2kb,因此分别命名为OPZ-131400、OPY-071000、OPY-042000。OPZ-131400和OPY-071000的克隆和鉴定工作已经完成,其中OPY-071000转换成了稳定的SCAR标记。3个突变系进行RAPD分析,彼此之间没有发现扩增产物的差异,它们与原始对照之间却有着共同的差异,表明长荚型突变品系在DNA分子水平上已接近同源。

Based on sequencing analyses of a codominant SCAR marker SCW 05 660 tightly linked to SMV resistance gene Rsa, two pairs of specific SCAR primers were designed and synthesized to amplify genomic DNA from 30 cultivated soybean accessions. Fingerprinting results showed that shorter fragments S 5 600 and S 05 660 were typical bands in disease resistance varieties, while longer fragments S 5 1000 and S 5 1600 were typical bands in susceptible accessions. Southern blot hybridization...

Based on sequencing analyses of a codominant SCAR marker SCW 05 660 tightly linked to SMV resistance gene Rsa, two pairs of specific SCAR primers were designed and synthesized to amplify genomic DNA from 30 cultivated soybean accessions. Fingerprinting results showed that shorter fragments S 5 600 and S 05 660 were typical bands in disease resistance varieties, while longer fragments S 5 1000 and S 5 1600 were typical bands in susceptible accessions. Southern blot hybridization results showed that these fragments generated by the two pairs of SCAR primers were homologous.

依据与SMV(SoybeanMosaicVirus)抗性基因紧密连锁的共显性SCAR标记SCW—05660的序列测定结果,合成了两对SCAR(SequenceCharacterizedAmplifiedRe-gions)标记特异引物,对30个栽培大豆品种进行了指纹图谱分析。结果表明,较短的片段S—5600和S—05660是抗病品种的特征性条带;而较长的片段S—51000和S—51600是感病品种的特征性条带。Southern杂交结果表明,这两对引物扩增出的条带具有同源性。

RAPD marker (OPH17(1400)) and SCARmarkers (SCAR1400 and SCAR(1265)) as well as fluorescence in situ hybridization (FISH) technique were used to detect wheat powdery mildew resistance gene Pm21 derived from Haynaldia villosa (6AL/ 6VS) in different genetic background of wheat breeding program. Among a total of 372 RAPD amplification reactions, 28 reastions (7.53%) yield no ampliflcation products, and the amplification products of 21 reactions (5.64%) were difficult to interpret the presence...

RAPD marker (OPH17(1400)) and SCARmarkers (SCAR1400 and SCAR(1265)) as well as fluorescence in situ hybridization (FISH) technique were used to detect wheat powdery mildew resistance gene Pm21 derived from Haynaldia villosa (6AL/ 6VS) in different genetic background of wheat breeding program. Among a total of 372 RAPD amplification reactions, 28 reastions (7.53%) yield no ampliflcation products, and the amplification products of 21 reactions (5.64%) were difficult to interpret the presence or absence of the OPH17(1400), indicating that RAPD marker OPH17(1400) is less reproducible and reliable, so that its usage in breeding program is limited. However, SCAR markers, SCAR(1400) and SCAR(1265), were amplified in all 488 PCR reactions, suggesting that SCAR markers are highly reproducible and reliable, and can be used in breeding Program. The specific SCAR(1265) made it possible to screen a large number of genotypes reliably and rapidly in practical breeding programs for the presence/absence detection between the resistant and susceptible plants. After backcrossed with common wheat lines for several bines, no recombination between the 6VS chromosome of Haynaldia villosa and any of the wheat chromosomes was detected by using FISH.

利用小麦抗白粉病基因Pm21的RAPD标记(OPH17(1400))、SCAR标记(SCAR(1400)和SCAR(1265))和荧光原位杂交技术(FISH)对小麦抗病育种材料中的抗白粉病Pm21基因进行了分子鉴定和标记辅助选择。利用随机引物OPH17进行RAPD分析结果表明,在3~5次重复共372次RAPD扩增中,有28次(7.53%)未获得扩增产物,有21次(5.64%)扩增结果难以判断目标片段OPH17(1400)的有无,说明RAPD标记检测结果的可靠性和重现性较差,在育种中应用有一定局限性。而在利用SCAR标记共488次PCR扩增中,均可以扩增出与Pm21基因连锁的多态性SCAR(1400)或SCAR(1265)目标片段,说明SCAR标记是稳定、准确、可靠的DNA分子标记,可应用于育种群体中Pm21基因的分子鉴定和标记辅助选择。利用RAPD和SCAR标记对具有不同遗传背景的"滚动式加代回交转育"抗白粉病育种群体中抗病基因Pm21分子标记检测结果表明,DNA分子标记与植株的白粉病抗性表现一致,并在此基础上进行了标记辅助的向农艺亲本的回交转育。荧光原位杂交结果表明,经多代回文改良,未发现簇毛麦6VS染色体...

利用小麦抗白粉病基因Pm21的RAPD标记(OPH17(1400))、SCAR标记(SCAR(1400)和SCAR(1265))和荧光原位杂交技术(FISH)对小麦抗病育种材料中的抗白粉病Pm21基因进行了分子鉴定和标记辅助选择。利用随机引物OPH17进行RAPD分析结果表明,在3~5次重复共372次RAPD扩增中,有28次(7.53%)未获得扩增产物,有21次(5.64%)扩增结果难以判断目标片段OPH17(1400)的有无,说明RAPD标记检测结果的可靠性和重现性较差,在育种中应用有一定局限性。而在利用SCAR标记共488次PCR扩增中,均可以扩增出与Pm21基因连锁的多态性SCAR(1400)或SCAR(1265)目标片段,说明SCAR标记是稳定、准确、可靠的DNA分子标记,可应用于育种群体中Pm21基因的分子鉴定和标记辅助选择。利用RAPD和SCAR标记对具有不同遗传背景的"滚动式加代回交转育"抗白粉病育种群体中抗病基因Pm21分子标记检测结果表明,DNA分子标记与植株的白粉病抗性表现一致,并在此基础上进行了标记辅助的向农艺亲本的回交转育。荧光原位杂交结果表明,经多代回文改良,未发现簇毛麦6VS染色体臂与普通小麦染色体的重组。

 
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