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   ribosome binding site 的翻译结果: 查询用时:0.01秒
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ribosome binding site
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  “ribosome binding site”译为未确定词的双语例句
     Sequencing of the DNA fragment revealed an open reading frame encoding 461 amino acids, homologous to known cbbM genes, with a ribosome binding site (RBS) upstream of cbbM and a terminator downstream of cbbM, without promoter.
     结果表明该DNA片段存在一个完整的阅读框架(ORF),即formⅡ RubisCO基因(cbbM),长为1386个核苷酸,共编码461个氨基酸。
短句来源
     The other ORF of 384 amino acids named ORF 1155 was related to outer membrane protein, but the ribosome binding site was not find from its upstream before initial start codon.
     ORF1155读框则与外膜蛋白相关,但起始密码子上游未发现RBS(可能与起始密码子上游序列长度太短有关),因此无法判断是否是一个真正的ORF;
短句来源
     The 5' untranslated leader region is 194bp, the putative ribosome binding site in this region is GGAGG, located immediately upstream of the start codon.
     5’前导区长194bp,其中SD序列为GGAGG,紧邻起始密码子上游.
短句来源
     According to the nucleotide sequence, the CS3 subunit gene has a typical ribosome binding site(rbs). and -10 as well as -35 sequences of the CS3 promoter upstream from the translation start sue.
     CS3亚基结构基因的核苷酸序列分析表明,在其翻译起始位点的上游存在着rbs位点及原核启动子的—10区和—35区DNA序列。
短句来源
     One of the fragments (called SP2) contains both sequences of promoter and ribosome binding site (RBS),while the another fragment (called SP1) contains not only promoter and RBS but also the signal peptide coding sequence.
     第二种不仅含有第一种DNA片段的全部序列,而且还有贮藏蛋白的信号肽编码序列(SP1)。
短句来源
  相似匹配句对
     protein binding;
     蛋白结合;
短句来源
     Effect of Ribosome Binding Site Sequence on the Expression Level of HBcAg in E. colt
     核糖体结合位点序列对大肠杆菌中HBcAg重组质粒表达的影响
短句来源
     EF1α·GTP catalyzes the binding of aminoacyl-tRNA to the A-site of the ribosome.
     GTP催化氨酰tRNA结合到核糖体的A位点。
短句来源
     On Binding And Layout of Periodicals
     新与美的交融——浅谈期刊的装帧设计
短句来源
     The quantity of ribosome was reduced.
     核糖体数量明显减少等。
短句来源
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  ribosome binding site
coli, in spite of there being no obvious ribosome binding site upstream.
      
The lyase structural gene follows a non-coding region with an inverted repeat and a ribosome binding site.
      
The two genes are transcribed on a 1.25 kb dicistronic transcript, and each coding region is preceded by a prokaryotic ribosome binding site consensus sequence.
      
It is flanked by a ribosome binding site on the 5' end and a potential transcription terminator on the 3' end.
      
A potential ribosome binding site is located 6 nucleotides upstream from the initiation codon and there are two sets of putative promotor sequences in the 5' flanking region.
      
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Sequence structure of Hepatitis B virus (HBV adrNC-l) DNA. was analyzed by computer to compare its sequence homology with other related DNA and search for restriction enzyme sites, reading frame, feature sequence of eukaryotic organism, hairpin loop and eukaryotic ribosome binding sites,etc. It proved that using computer to analyze nucleic acid sequence is one of the important technics in molecular biology research, since these data were obtained with VAX-11/730 computer in ou institute.

以乙肝病毒DNA序列分析为例,较详细地介绍了电子计算机在分析DNA序列的同源性,寻找内切酶识别位点,开放读码框架,真核生物的特征顺序,发卡环,真核核糖体RNA结合位点等方面所进行的工作,这些工作是在我所VAX-11/730计算机上完成的。

The conserved regions in promoters, ribosome-binding sites, teminator sequence and splice sites for each category of sequences are obtained by statistical analysis. On account of the possible important rule of these conserved sites on the regulation of gene expression we have calculated the lecal deviations of double helix structures of DNAs and compared them with conserved sites.

统计分析了12类核酸序列在起始密码子邻近,终止密码子邻近以及内含子两端的保守区,得到了保守区随序列类别变化的经验规律,鉴于这些保守位点对基因表达的调控可能具有重要作用,本文利用Tung-Harvey模型,计算了核酸双螺旋结构的局部偏差,企图发现局部偏差相对较大的位点与保守位点之间的关系。

The recombinant (pKL series) Plasmids showing different levels of HBcAg antigenicity in ELISA were constructed by inserting the HBcAg gene which was randomly deleted at the non-coding region by Ba/-31 exonuclease into plasmid vector pKK223-3 containing tac promoter and SD sequence. SDS-PAGE and Western blot experiments indicated that molecular weight of the HBcAg protein generated by these positive clones was 21000D. Three plasmids with high, moderate and low HBcAg expression level were sequenced and the distance...

The recombinant (pKL series) Plasmids showing different levels of HBcAg antigenicity in ELISA were constructed by inserting the HBcAg gene which was randomly deleted at the non-coding region by Ba/-31 exonuclease into plasmid vector pKK223-3 containing tac promoter and SD sequence. SDS-PAGE and Western blot experiments indicated that molecular weight of the HBcAg protein generated by these positive clones was 21000D. Three plasmids with high, moderate and low HBcAg expression level were sequenced and the distance between SD sequence and HBcAg gene ATG codon was 12, 13 and 19bp respectively. Computer analysis of secondary structure of the ribosome binding sites on RNAtranscripts also revealed energy and structure differences between the low and high level expression plasmids, suggesting the importance of this distance and the mRNA structure to gene expression.

用Bal-31外切酶调整乙肝病毒核心抗原(HBcAg)基因非编码区长度并克隆到质粒pKK 223-3中,获得了不同水平地表达HBcAg的重组质粒pKL系统。该系统所表达的HBcAg蛋白分子量为21000D。DNA序列分析发现高、中、低表达水平的3个重组质粒的SD序列到HBcAg基因的ATG之间的距离分别为12bp、13bp和19bp,高、低表达质粒的mRNA核糖体结合位点序列的二级结构分析显示,二者的自由能相差约3倍,且低表达质粒的mRNA的SD序列中的3个碱基及AUG中的3个碱基都参与配对,而高表达质粒只有SD序列中的2个碱基参与配对,表明SD序列到ATG的距离及mRNA的二级结构均在HBcAg的表达调控中起重要作用。

 
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