Twenty rabbits were injected with 12% monobasic sodium phosphate (5ml/kg body weight) through vein in ear edge. The changes of the electrocardiogram and electrolyte (K~+, Cl~-, Ca~(++)), before injection and 20, 60, 120 minutes and 4—5 hours after injection, were observed.
(2) availabilities of phosphorus: 90.3% in monobasic sodium phosphate, 90.3% in monobasic calcium phosphate, 60.3% in dibasic calcium phosphate, and 2.8% in tribasic calcium phosphate.
Method: Hypericin content in Yufulai capsulaes was determined by HPLC. The lichrospher C 18 column was used, The mobile phase consisted of 1.56% monobasic sodium phosphate (pH was adjusted to 2.1 with phosphoric acid) methanol acetonitrile(1∶7∶2). The flow rate was 1.0 ml·min -1 . The detecting wavelength was at 590 nm.
Method The column:Hypersil Phenyl 2(5μ)(250 mm×4.6 mm). The mobile phase:0.5% monobasic sodium phosphate in water(pH 4.0)-acetonitrile(60 ∶ 40),Add 2 ml of triethylamine per liter, and mix.
The mobile phase consisted of methanol-sodium phosphate buffer [monobasic sodium phosphate (MW=119.98) 1.7997 g and phosphoric acid (85%) 1.02 mL, combined and brought the total volume of 1 000 mL with water] (89:11); the mobile phase was maintained at a flow-rate of 0. 8 mL per minute; the column was maintained at 40℃;
In living animals this structure can be induced by the addition of sodium cacodylate, monobasic sodium phosphate, dibasic sodium phosphate, sucrose, calcium chloride, or formaldehyde to the sea water.
Twenty rabbits were injected with 12% monobasic sodium phosphate (5ml/kg body weight) through vein in ear edge. The changes of the electrocardiogram and electrolyte (K~+, Cl~-, Ca~(++)), before injection and 20, 60, 120 minutes and 4—5 hours after injection, were observed. The results showed as follows: the alteration of P wave, Q—T interval, S—T section and T wave are more evident, especially the descent of S—T section and high point of T wave are very notable; by blood-gas analysis, the ...
Availabilities of calcium and phosphorus in nutritive salts for grass carp, as determined by the chromic oxide indicator method, were as follows: (Ⅰ) availabilities of calcium: 81.3% in calcium lactate, 26.4% in calcium carbonate, 69.7% in monobasic calcium phosphate, 39.9% in dibasic calcium phosphate, and O in tribasic calcium phosphate; (2) availabilities of phosphorus: 90.3% in monobasic sodium phosphate, 90.3% in monobasic calcium phosphate, 60.3% i...
Objective:A RP-HPLC method was developed for the determination of Amphotericin B in Amphotec Method:In the method,Hypersil BDS C 8 as the stationary phase,and 0 01 mol·L -1 monobasic sodium phosphate-acetonitrile(67∶33)as mobile phase,detected at 405 nm Results:Within 5~50 μg·mL -1 ,Amphotericin B had a good linearity( r =0 999 9) The average recovery of Amphotericin B was 100 3%,with RSD was 0 83%( n =4) Conclusion:The method is simple,accurate and easy to do