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cellulose-binding domains
相关语句
  纤维素结合结构域
     This paper reviewed and discussed the recent advance in structure-function of cellulase from bacteria by structure biology and protein engineering, including cellulase compose, the structure-function of catalytic domains and cellulose-binding domains and linker, molecular folding, and the research prospects.
     主要探讨了近年来利用结构生物学和蛋白质工程技术在细菌纤维素酶分子结构和功能方面研究的进展,包括细菌纤维素酶的组成、催化域、纤维素结合结构域和连接桥的结构和功能以及分子折叠,并展望了该领域的研究前景。
短句来源
  相似匹配句对
     They are DNA binding domains.
     它们的C末端分别含有SAP和SANTDNA结合结构域。
短句来源
     Progress in Research on DNA-Binding Protein Domains
     DNA结合蛋白结构域的研究进展
短句来源
     Advances on cellulose binding domain of microbiological cellulase
     纤维素酶分子的纤维素吸附区的研究进展
短句来源
     Cellulose binding domains improve the binding and facilitate the activity of catalytic domains on the insoluble substrate,but not on soluble substrate.
     纤维素吸附区促进酶与底物的结合 ,有利于催化区对不溶性底物的作用 ,但对可溶性底物的催化作用无影响。
短句来源
     Starch-binding domains(SBD)are present in lots of amylases,e.
     很多淀粉酶中都具有淀粉结合区域(starch-bindingdomain,SBD),如!
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  cellulose-binding domains
A method for the gram-scale production of cellulose-binding domains (CBD) through the proteolytic digestion of a commercial enzymatic preparation (Celluclast) was developed.
      
Large-scale production of cellulose-binding domains.
      
Two cellulose-binding domains from Clostriium cellulovorans were fused to form a cellulose crosslinking protein (CCP).
      
Cellulose-binding domains have been isolated from various cellulases, and proteins, which lack hydrolytic activity.
      
Isolated cellulose-binding domains bear most of the binding properties of cellulases (quasi-irreversibility and dispersive effect) but do not hydrolyse cellulose.
      
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This review presents the structure and function of cellulose-binding domains,xylan-binding domains linker sequence and repeated sequences of xylanases. It also in-troduce sorne active site amino acids, three-dimensional structure of xylanases and site-directed mutagenesis to xylanases.

本文详细叙述了木聚糖酶分子的催化区域、纤维素结合区域、木聚糖结合区域、连接序列与重复序列的结构和功能方面的研究进展,并介绍了木聚糖酶分子中常见的活性位点氨基酸以及该酶的定点诱变和三级结构。

The enzymatic degradation of cellulose, the maior organic carbon source in the biosphere, is essential for ednwining the global carbon cycle. Most of cellulases have catalytic doedns and celluose binding dornains, which can bind to cellulose and are conserved in amino acid Sequences. With comparison of each cellulose binding domain, their structure, function and binding mechanism are clearer, with the utilization of small angle X-ray scattering, nuclear magnetic are and hydrophobic cluster analysis.This...

The enzymatic degradation of cellulose, the maior organic carbon source in the biosphere, is essential for ednwining the global carbon cycle. Most of cellulases have catalytic doedns and celluose binding dornains, which can bind to cellulose and are conserved in amino acid Sequences. With comparison of each cellulose binding domain, their structure, function and binding mechanism are clearer, with the utilization of small angle X-ray scattering, nuclear magnetic are and hydrophobic cluster analysis.This review outlined the recen advnces of this area.

纤维素是生物界中最重要的碳源物质,其生物降解对维持地球上的碳素循环至关重要。其降解涉及到一系列的纤维素酶,是一个多酶协同的过程。近年来相继发现纤维素酶大都含有催化区和可与纤维素结合且氨基酸序列较为保守的功能区,即纤维素吸附区(cellulosebindingdomain,CBD)。通过序列之间的比较,用小角度X射线衍射、核磁共振及流水基簇分析等方法,在CBD的结构、功能及与纤维素的吸附机制等方面有了较深人的了解。本文为近十年来此领域研究进展的简述。

Bacteriophages capable of binding cellulose matrix were screened from a 15mer phage peptides library. In the deduced amino acid sequences of the screened phages, a characteristic region containing a conserved aromatic residue [tyrosine (Y) or phenylalanine (F)] was found, which is similar to the normal cellulose binding domains found in fungal and bacterial cellulose catalysase. DotELISA showed that the phage containing sequence as SWYL has higher affinity to cellulose fibre than other phages, such as...

Bacteriophages capable of binding cellulose matrix were screened from a 15mer phage peptides library. In the deduced amino acid sequences of the screened phages, a characteristic region containing a conserved aromatic residue [tyrosine (Y) or phenylalanine (F)] was found, which is similar to the normal cellulose binding domains found in fungal and bacterial cellulose catalysase. DotELISA showed that the phage containing sequence as SWYL has higher affinity to cellulose fibre than other phages, such as those containing sequences as CWYGNC, CWYGEC and XSWYDXXSWFSX. Results indicated that SWYL may be a good candidate for cellulose binding motif. This work laid basis for further study on cellulose binding motif.

从编码随机15肽序列的噬菌体表面展示文库中,筛选得到了与纤维素有特异亲和能力的噬菌体克隆。序列分析结果表明,这些噬菌体克隆编码的氨基酸序列中芳香族氨基酸残基高度保守,与天然纤维素结合结构域的结构有一定的类似性。利用重组噬菌体研究的结果表明,包含SWYL序列的噬菌体克隆与纤维素的结合能力最强,而其他包含类似结构的噬菌体克隆,包括有两个半胱氨酸的噬菌体克隆(XCWYGNCX、XCWYGECX)及含两个类似结构的噬菌体克隆(XSWYDXXSWFSX)与纤维素的特异亲和能力较低。初步的实验结果显示SWYL序列具有一定的纤维素结合能力,为纤维素结合结构基元的进一步研究打下基础。

 
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