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hepatitis delta antigens
相关语句
  相似匹配句对
     STUDIES ON CELLULAR IMMUNITY OF DELTA HEPATITIS
     HDV感染者细胞免疫状态的研究
短句来源
     The Molecular Biology of Hepatitis delta virus
     丁型肝炎病毒的分子生物学研究进展
短句来源
     Die Hepatitis G
     庚型肝炎
短句来源
     THE FAULT DELTA
     断块型三角洲
短句来源
     Hepatitis C and Detection for it
     丙型肝炎及其检测
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A 27-peptide,a fragment of hepatitis delta antigen(HDAg),was synthesized and used to develop an ELISA method for ihe detection of anti-HD.It was found that positive anti-HD reaction occurred between the coated 27-peptide and a stored sample of serum which was known anti-HD positive.Absorption test revealed that the synthetic peptide competed with natural HDAg for anti-HD,suggesting that the peptide possessed the antigenicity similar to that of natural HDAg.The antigenicity of the synthetic peptide was...

A 27-peptide,a fragment of hepatitis delta antigen(HDAg),was synthesized and used to develop an ELISA method for ihe detection of anti-HD.It was found that positive anti-HD reaction occurred between the coated 27-peptide and a stored sample of serum which was known anti-HD positive.Absorption test revealed that the synthetic peptide competed with natural HDAg for anti-HD,suggesting that the peptide possessed the antigenicity similar to that of natural HDAg.The antigenicity of the synthetic peptide was quite specific wihtout cross reaction with normal human and mouse sera and with anti-HA.anti-HB and anti-HC sera.Among 300 blood donors,there was only 1 case(0.33%)anti-HD positive with an ALT level 2 times higher than normal.In 41 cases of non-B hepatitis and 52 cases of HAV hepatitis,none was anti-HD positive.In 211 cases of various types of HBV hepatitis,21 were(9.95%)anti-HD positive,among whom 2/82(2.5%)werehealthy HBV carriers,6/43(13.95%)were patients with a-cute icteric hepatitis,6/60(10.00%)were patients of chronic active hepatitis,4/18(22.20%)were patients of severe hepatitis,and 3/8(37.50%)were those with liver cirrhosis.These results were consistent with those in our previous reports.

本文选择、设计并合成了含天然HDAg上的部分片段的27肽,用于建立检测抗HD的ELISA方法。试验结果表明,包被的合成肽抗原与已知抗HD阳性冻存血清呈抗HD阳性反应;它能与天然HDAg竞争抗HD,具天然HDAg上相应片段的抗原活性;该合成肽抗原专一性强,与正常人血清,正常小鼠血清,单纯甲型、乙型、丙型肝炎抗体阳性血清无免疫交叉反应。用建立的ELISA法,检测1992年1~5月的部分献血员及住本科各型肝炎患者血清标本的抗HD。300例献血员中1例抗HD阳性(0.33%),其ALT增高2倍以上。非乙型肝炎41例,甲型肝炎52例,抗HD均阴性。各型乙型肝炎211例,抗HD阳性21例(9.95%)。其中,携带者2/82例(2.5%);急黄肝6/43例(13.95%);慢活肝6/60例(10.00%);重肝4/18例(22.20%);肝炎肝硬化3/8例(37.50%),结果与本科已往报告基本一致。

The antibody to hepatitis delta virus (Anti-HD) from the serum positive for HBsAg and Anti-HD was fractionated with ion-exchange chromatography of DE-52 and labelled with horseradish peroxidase (HRP). Hepatitis delta antigen (HDAg) from the liver of the woodchuck hepatitis virus (WHV) carrier (woodchuck infected with HDV) was purified through Sepharose 4B column and affinity chromatography column. Its titre was 10~(-6) determined with double antibody sandwich method. Anti-HD in serum...

The antibody to hepatitis delta virus (Anti-HD) from the serum positive for HBsAg and Anti-HD was fractionated with ion-exchange chromatography of DE-52 and labelled with horseradish peroxidase (HRP). Hepatitis delta antigen (HDAg) from the liver of the woodchuck hepatitis virus (WHV) carrier (woodchuck infected with HDV) was purified through Sepharose 4B column and affinity chromatography column. Its titre was 10~(-6) determined with double antibody sandwich method. Anti-HD in serum could be detected with ELISA competitive method. The results of specific, sensitive and repeating tests of the reagent were identical with those of Delta assay Ab (Noctech Co., Ltd. Ireland)

取乙型肝炎患者Delta抗体(抗—HD)阳性血清,经硫酸铵盐析和DE—52柱层析,得到纯化的抗—HD抗体,用辣根过氧化物酶标记。用丁型肝炎病毒(HDV)感染慢性携带土拨鼠肝炎病毒的东方土拨鼠(Woodchuck)后,取其肝组织制成匀浆,离心后经Sepharose 4B柱,免疫亲和层析柱,获得高丰度的HDAg。双抗体夹心法测定其滴度高达1∶10~6。制备成ELISA竞争抑制法检测抗HD试剂盒,其特异性、敏感性、重复性与爱尔兰同种试剂比较结果一致。

Chinese hepatitis delta antigen-coding cDNA was characterized by cloning and sequencing four fragments with size of 707bp, 414bp, 376bp and 274bp respectively, from a HBsAg carrier positive for HDAg and HDV RNA from Western China Sichuan Province, using reverse trans-cription and polymerase chain reaction. Comparison of homology of the Chinese HDAg-coding sequence with those of other known isolates: America ( Makino ) , Italia ( Wang ) , United Kindom ( Saldanha ) , Taiw-an(Chao)is 99.4%, 92.1%, 94.3%,...

Chinese hepatitis delta antigen-coding cDNA was characterized by cloning and sequencing four fragments with size of 707bp, 414bp, 376bp and 274bp respectively, from a HBsAg carrier positive for HDAg and HDV RNA from Western China Sichuan Province, using reverse trans-cription and polymerase chain reaction. Comparison of homology of the Chinese HDAg-coding sequence with those of other known isolates: America ( Makino ) , Italia ( Wang ) , United Kindom ( Saldanha ) , Taiw-an(Chao)is 99.4%, 92.1%, 94.3%, 91.4% for nucleotide and 99.1%, 87.9%, 88.2%, 89.3% for amino acid respectively, and four conserved re靑ons were located.

从我国四川一丁型肝炎病毒抗原(HDAg)、HDV RNA双阳性的HBsAg携带者血清中提取RNA,采用人工合成的引物进行4次逆转录和聚合酶链反应(PCR),获得了长度分别为707bp、414bp、876bp和274bp的4个HDV cDNA片段,并对其进行了克隆和序列分析。其抗原编码区核苷酸序列与美国(Makino)、意大利(Wang)、英国(Saldanha)和我国台湾株(Chao)等株比较同源性,分别为99.4%、92.1%、94.3%和91.4%,由它推导的氨基酸序列的同源性分别为99.1%、87.9%、88.2%和89.3%,并发现了4个集中保守的区域。

 
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