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primary trophoblast
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  “primary trophoblast”译为未确定词的双语例句
     Results: None of the three monoclonal antibodies (CD133-1, CD133-2, CD133-2/AC141) to CD133 can react with primary trophoblast and JAR cells before methanol fixation. After fixed with methanol, only CD133-2 mAb (clone number AC141) can react with human trophoblastic cells.
     固定后 ,抗CD133 1(AC133 1 APC)、抗CD133 2 (2 93C3 PE)仍不能与滋养细胞反应 ,而抗CD133 2 (AC14 1 PE)与滋养细胞胞内抗原结合。
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     So the experiments on cultured trophoblast cells should be performed. In the present study , we were about to verify evidences of HBV infection in the cells by morphological and pathological methods and fumble about a feasible method of HBV infection in vitro by experiment on a hepatical cell line namely HepG2 cells,then tested the virus infection in cultured primary trophoblast cells in vitro.
     因此必须进行HBV感染离体培养滋养层细胞的实验,本项研究先对在体情况下HBV感染人胎盘滋养层细胞的情况作验证性研究,再以文献报道的实验方法为依据,用肝源性的HopG2细胞为靶细胞,摸索较为可行的HBV体外感染细胞实验方法。 以此方法进行滋养层细胞的HBV感染实第。
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     (3)The experiment of HBV infectionin cultured primary trophoblast cells in vitro were performed by the same methods, moreover transmission electron microscopy and immunoelectron microscopic method were used to confirm the results.
     ③用HBV体外感染HePGZ细胞同样的方法进行HBV体外感染人胎盘滋养层细胞的实验与检测。
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     (3)In the experiment of HBV infection in cultured primary trophoblast cells in vitro, the results was similar to that of the experiment on HepG2 cells.
     ③ELISA检测人胎盘滋养层细胞感染实验各组培养液上清HBSAg、免疫组化法检测细胞内 HBSAg、细胞裂解后 PCR法检测细胞内 HBV DNA的实验结果都涕。
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     Cultured primary trophoblast cells might also be infected by HBV if the cells were incubated with HBV positive serum, 2%PEG and 1%DMSO or with HBV positive serum and 2%PEG in
     而在离体培养的情况下,滋养层细胞在乙型肝炎患者血清、2剁EG、1汕(或不加入DMSO)加入完全培养基中组成的培养系统中培养24小时也可能形成能够检测到的感染。 但本项研究仅为一初步研究,由于人胎盘滋养层细胞为非肝源性细胞,所以对其与HBV的相互作用情况尚需进一步验证并加以深入研究。
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  相似匹配句对
     THE PRIMARY OF GALLBLADDEY
     原发性胆囊癌——附8例报告并文献复习
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     primary prevention.
     初级预防。
短句来源
     In vitro primary cell culture of human trophoblast from first trimester pregnancy
     一种简便人绒毛滋养细胞体外原代培养方法的建立
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     Syncytin and fusion of trophoblast
     Syncytin与滋养细胞融合
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  primary trophoblast
This is a novel technique for purifying primary trophoblast cultures and may have wider applicability in cell culture of other cell types.
      
It is suggested that cell binuclearity at the morula stage is a possible way to polyploidization of nuclei, resulting in the formation of primary trophoblast giant cells.
      
When blastocysts interacted with the uterine epithelial cells, P4 promoted the attachment and invasion of the primary trophoblast giant cells.
      
The accepted course of action dictates that you use primary trophoblast cell cultures derived from human placentas.
      
Time-course of apoptosis-inducing activities of human anti-annexin V or negative control antibodies on primary trophoblast cells.
      
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Implantation of the mouse embryo is dependent on the interactions between the trophoblast cells and the surrounding uterine environment. The initial invasion by primary trophoblast stimulates the uterine stromal fibroblasts differentiate into deci-dual cells,which deposit a peri cellular matrix consisting of LN, FN and Col Ⅳ. The secondary trophoblast giant cells (TGCs) from ectoplacental cone (EPC) invade the decidua to form the fetal portion of the placenta. We used synthetic peptides cyclic YIGSR...

Implantation of the mouse embryo is dependent on the interactions between the trophoblast cells and the surrounding uterine environment. The initial invasion by primary trophoblast stimulates the uterine stromal fibroblasts differentiate into deci-dual cells,which deposit a peri cellular matrix consisting of LN, FN and Col Ⅳ. The secondary trophoblast giant cells (TGCs) from ectoplacental cone (EPC) invade the decidua to form the fetal portion of the placenta. We used synthetic peptides cyclic YIGSR (cYIGSR) and RGDS to study the mechanisms of EPC cells interaction with LN. The results indicated that cYI-

本文用LN及含其活性位点序列的合成肽段cYIGSR和RGDS对小鼠EPC细胞与LN的相互作用机制进行研究。结果表明:合成肽段cYIGSR和RGDS能促进EPC粘附并具协同效应。cYIGSR还能促进EPC扩展与次生TGCs迁移。LNA链上RGD和B_1链上YIGSR两个活性位点协同地参与了LN对EPC的粘附、扩展以及次生TGCs的迁移的促进作用。cY+R合用不能完全竞争性抑制EPC与LN的结合,说明还有其他作用位点存在。

Objective: To provide a new intracellular marker to characterize human trophoblastic cell.Methods: Human first-trimester trophoblastic cells were isolated by trypsin/DNase I digestion and discontinuous Percoll density gradient centrifugation. Identified by immunocytochemistry and FACS with monoclonal antibody (mAb) to cytokeratin, the trophoblastic cells were more than 95% pure. The CD133 expression in the trophoblast and JAR cells was analyzed by FACS after methanol fixation. Results: None of the three monoclonal...

Objective: To provide a new intracellular marker to characterize human trophoblastic cell.Methods: Human first-trimester trophoblastic cells were isolated by trypsin/DNase I digestion and discontinuous Percoll density gradient centrifugation. Identified by immunocytochemistry and FACS with monoclonal antibody (mAb) to cytokeratin, the trophoblastic cells were more than 95% pure. The CD133 expression in the trophoblast and JAR cells was analyzed by FACS after methanol fixation. Results: None of the three monoclonal antibodies (CD133-1, CD133-2, CD133-2/AC141) to CD133 can react with primary trophoblast and JAR cells before methanol fixation. After fixed with methanol, only CD133-2 mAb (clone number AC141) can react with human trophoblastic cells. The AC141-positive cells are also cytokeratin7- positive. Conclusion: The CD133-2/AC141 can be used as a useful marker to identify the purity of primary trophoblastic cells.

目的 了解CD133在滋养细胞的表达情况 ,为鉴定滋养细胞株及原代分离的滋养细胞提供新的细胞内标志。 方法 通过胰蛋白酶 /DNaseⅠ分步消化 ,Percoll密度梯度离心分离纯化滋养细胞 ,经抗角蛋白 (CK 7)、波形蛋白 (vimentin)单克隆抗体行免疫细胞化学和流式细胞仪 (FACS)鉴定 ,纯度达 95 %以上。采用三种不同的CD133荧光标记单克隆抗体在70 %的甲醇固定前后对原代分离的滋养细胞和绒毛膜上皮癌细胞株 (JAR)行FACS分析。 结果  70 %甲醇固定前 ,三种CD133单克隆抗体均不能与滋养细胞反应 ;固定后 ,抗CD133 1(AC133 1 APC)、抗CD133 2 (2 93C3 PE)仍不能与滋养细胞反应 ,而抗CD133 2 (AC14 1 PE)与滋养细胞胞内抗原结合。而且AC14 1阳性的细胞也是CK 7阳性的细胞。 结论 CD133 2 /AC14 1可作为滋养细胞纯度鉴定的一种有用标志 ,但应用时应选择合适的抗体。

 
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