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fractional cultivation
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  “fractional cultivation”译为未确定词的双语例句
     The 9 randomly selected hip liquid were proceeded fractional cultivation,1 share was segregated coxsacklie virus,and confirmed was CoxB 3 in adopted neutralization tests,recognized through electron microscope.
     随机抽样 9例作病毒分离培养 ,1例分离出柯萨奇病毒 ,采用中和试验证实为柯萨奇B组 3型病毒 ,并经电子显微镜检查确认。
短句来源
     METHODS: The experiment was conducted in Department of Clinical Laboratory, Anyang People's Hospital and Clinical Laboratory, Anyang Tumor Hospital from March to October 2004. The fresh femoral bone marrow of 13-18 weeks conceptus age embryo by induction of labor with water bag were fractional cultivation by holo-marrow direct inoculation (the sample used in this experiment with the knowledge and agreement of provider).
     方法:实验于2004-03/10在安阳市人民医院检验科和安阳肿瘤医院临床实验室完成。 采用全髓直接接种法分离培养13~18周胎龄水囊引产新鲜胎儿股骨骨髓(获提供者知情同意标本用于此实验)。
短句来源
     Objective:To explore the methods of fractional cultivation and purification of olfactory ensheathing cells (OECs) from human embryo with immune adsorption in vitro.
     目的:探索分离培养人胚嗅鞘细胞以及纯化的方法。
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     角朊细胞培养技术最新进展
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     FRACTIONAL(g,f)-FACTORS IN GRAPHS
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     FRACTIONAL k-FACTORS OF GRAPHS
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Objective:To discuss the effect and significance of coxsacklie virus B3 in the occurrence and development of acute transient synovitis of the hip in children.Methods:100 cases of acute transient synovitis of the hip,in these cases,79 were detected with sero immunity method(ELISA),21 were detected by virology in hip liquid,who were suspected coxsacklie virus infected,randomly selected 9 cases to segregate virus from hip liquid.Results:79 Children acute transient synovitis of the hip had 11 positive reaction...

Objective:To discuss the effect and significance of coxsacklie virus B3 in the occurrence and development of acute transient synovitis of the hip in children.Methods:100 cases of acute transient synovitis of the hip,in these cases,79 were detected with sero immunity method(ELISA),21 were detected by virology in hip liquid,who were suspected coxsacklie virus infected,randomly selected 9 cases to segregate virus from hip liquid.Results:79 Children acute transient synovitis of the hip had 11 positive reaction of CoxB lgM through seroimmunity method,14 positive reaction of AdV lgM,8 positive reaction of CMV lgM,8 positive reaction of RSV lgM,38 negative reaction.21 cases of hip liquid had 7 positive reaction of CoxB,5 positive reaction of AdV,2 positive reaction of CMV,7 negative reaction.1 case both in blood and hip liquid had CoxB positive reaction,and 2 case Adv positive reaction.The 9 randomly selected hip liquid were proceeded fractional cultivation,1 share was segregated coxsacklie virus,and confirmed was CoxB 3 in adopted neutralization tests,recognized through electron microscope.Conclusion:CoxB 3 have the possibility to infect children and causing acute transient synovitis of the hip.Provide etiological methods for antiviral therapy.

目的 :探讨儿童急性暂时性髋关节炎与柯萨奇B组 3型病毒的相关性。方法 :采用ELISA法检测 10 0例急性暂时性髋关节滑膜炎患儿血清及髋关节液中柯萨奇B组病毒 (CoXB)、腺病毒 (AdV )、巨细胞病毒 (CMV)、呼吸道合胞病毒 (RSV)抗体 ,其中 79例作病毒血清免疫学检测 ,2 1例作髋关节病毒学检测 ,怀疑柯萨奇病毒感染者 ,随机抽样 9例作髋关节液中的病毒分离。结果 :急性暂时性髋关节滑膜炎患儿 79例血清免疫学检测查出Coxb lgM阳性 11例 ,AdV lgM阳性 14例 ,CMV lgM阳性 8例 ,RSV lgM阳性 8例 ,3 8例阴性。 2 1例髋关节液中查出CoXB阳性 7例 ,AdV阳性 5例 ,CMV阳性 2例 ,7例阴性。同时在血液和髋关节液中查出CoXB阳性 1例 ,AdV阳性 2例。随机抽样 9例作病毒分离培养 ,1例分离出柯萨奇病毒 ,采用中和试验证实为柯萨奇B组 3型病毒 ,并经电子显微镜检查确认。结论 :柯萨奇B组 3型病毒具有感染儿童导致急性暂时性髋关节滑膜炎的可能性。为采用抗病毒治疗提供了病原学依据

AIM: To observe the capability of fetus bone marrow mesenchyme stem cells in distinct transfer of culture power orientation inductive differentiation into osteoblast, which provides part of test parameter for the application of bone marrow mesenchyme stem cells in the bone tissue engineering.METHODS: The experiment was conducted in Department of Clinical Laboratory, Anyang People's Hospital and Clinical Laboratory, Anyang Tumor Hospital from March to October 2004. The fresh femoral bone marrow of 13-18 weeks...

AIM: To observe the capability of fetus bone marrow mesenchyme stem cells in distinct transfer of culture power orientation inductive differentiation into osteoblast, which provides part of test parameter for the application of bone marrow mesenchyme stem cells in the bone tissue engineering.METHODS: The experiment was conducted in Department of Clinical Laboratory, Anyang People's Hospital and Clinical Laboratory, Anyang Tumor Hospital from March to October 2004. The fresh femoral bone marrow of 13-18 weeks conceptus age embryo by induction of labor with water bag were fractional cultivation by holo-marrow direct inoculation (the sample used in this experiment with the knowledge and agreement of provider).When the adherence cells were confluence above 90%,then those were digested and went down to posterity.All passage cells were separated into 1×109 L-1 cell density, and then were inoculated in L-DMEM culture contained 0.1-volume fraction of fetus ox blood serum (FBS) to go on cultivation and passage. The osteogenic inducer desamethasone,β-sodium glycerophosphate and ascorbic acid were added in part of the culture medium, which were used to induce differentiation into osteoblast.Ten generations were passed on in this way. The formation of cells was observed under the invert microscope The expression of alkaline phosphatase (ALP), the marker enzyme of mature osteoblast, was detected by the cytochemistry staining The activity of ALP was ascertained by automatic biochemistry analyzer, by means of evaluating the capability of osteogenic differentiation from aniso-generation times cultivation cells.RESULTS: ① The positive percentage of ALP staining: In 7 generation times the capability of osteogenic differentiation from fetal bone marrow mesenchyme stem cells had insignificant difference, all over 87.5% (P > 0.05) It was 93.4% of the 3rd generation that was the most powerful. It was 72.7-51.3% from the 8th generation which reduced gradually. ② The activity of ALP: There was insignificant difference within the 7th generation (P > 0.05) It was (2 307.1±15.0) nkat/L of the 3rd generation that was the highest From the 8th generation, it decreased gradually. It was (905.2 ±10.0) nkat/L only at the 10th generation.CONCLUSION: The capability of osteogenic differentiation from bone marrow mesenchyme stem cells is different in different generation times, and it reduces significantly from the 8th generation. It is indicated that the bone marrow mesenchyme stem cells are ageing gradually after detaching the body in vivo environment. As the seed cell of the tissue engineering, the culture of bone marrow mesenchyme stem cells in vitro is not suitable over 7 generations.

目的:观察不同传代倍数胎儿骨髓间充质干细胞定向诱导分化为成骨细胞的能力,为骨髓间充质干细胞在骨组织工程中的应用提供部分实验参数。方法:实验于2004-03/10在安阳市人民医院检验科和安阳肿瘤医院临床实验室完成。采用全髓直接接种法分离培养13~18周胎龄水囊引产新鲜胎儿股骨骨髓(获提供者知情同意标本用于此实验)。贴壁细胞达90%以上融合时消化传代。传代细胞部分以1×109L-1的细胞密度接种于含体积分数为0.1的胎牛血清的L-DMEM的培养基中继续培养,传代;部分在培养基中添加成骨诱导剂地塞米松,β-甘油磷酸钠及抗坏血酸向成骨细胞诱导分化。如此连续传10代。倒置显微镜下观察细胞形态、细胞化学染色检测成熟成骨细胞的标志酶碱性磷酸酶的表达、全自动生化分析仪测定碱性磷酸酶活性,鉴定不同代次培养细胞成骨分化能力。结果:①碱性磷酸酶染色阳性百分率:传代7代以内胎儿骨髓间充质干细胞成骨分化能力无显著差异,均在87.5%以上(P>0.05);以第3代最强达93.4%。第8代后逐渐减弱,为72.7%~51.3%。②碱性磷酸酶活性:传代7代以内无差异(P>0.05),以第3代最高,为(2307.1±15.0)nkat/L,第8...

目的:观察不同传代倍数胎儿骨髓间充质干细胞定向诱导分化为成骨细胞的能力,为骨髓间充质干细胞在骨组织工程中的应用提供部分实验参数。方法:实验于2004-03/10在安阳市人民医院检验科和安阳肿瘤医院临床实验室完成。采用全髓直接接种法分离培养13~18周胎龄水囊引产新鲜胎儿股骨骨髓(获提供者知情同意标本用于此实验)。贴壁细胞达90%以上融合时消化传代。传代细胞部分以1×109L-1的细胞密度接种于含体积分数为0.1的胎牛血清的L-DMEM的培养基中继续培养,传代;部分在培养基中添加成骨诱导剂地塞米松,β-甘油磷酸钠及抗坏血酸向成骨细胞诱导分化。如此连续传10代。倒置显微镜下观察细胞形态、细胞化学染色检测成熟成骨细胞的标志酶碱性磷酸酶的表达、全自动生化分析仪测定碱性磷酸酶活性,鉴定不同代次培养细胞成骨分化能力。结果:①碱性磷酸酶染色阳性百分率:传代7代以内胎儿骨髓间充质干细胞成骨分化能力无显著差异,均在87.5%以上(P>0.05);以第3代最强达93.4%。第8代后逐渐减弱,为72.7%~51.3%。②碱性磷酸酶活性:传代7代以内无差异(P>0.05),以第3代最高,为(2307.1±15.0)nkat/L,第8代后逐渐降低,至第10代仅为(905.2±10.0)nkat/L。结论:不同代次的骨髓间充质干细胞诱导分化为成骨细胞的能力不同,8代以后明显减弱。提示脱离了体内环境后,骨髓间充质干细胞逐渐老化。做为组织工程的种子细胞,骨髓间充质干细胞体外培养不宜超过7代。

Objective:To explore the methods of fractional cultivation and purification of olfactory ensheathing cells (OECs) from human embryo with immune adsorption in vitro. Method:The olfactory bulb was separated from human embryo and monoplast suspension was harvested after trypsinization.Then the cells were seed in the culture dish custodited by p75 with DMEM/F12 medium containing Forskolin and BPE. Two weeks later, the cells were characterized by immunohistochemical staining. Result:The shape of the cultured...

Objective:To explore the methods of fractional cultivation and purification of olfactory ensheathing cells (OECs) from human embryo with immune adsorption in vitro. Method:The olfactory bulb was separated from human embryo and monoplast suspension was harvested after trypsinization.Then the cells were seed in the culture dish custodited by p75 with DMEM/F12 medium containing Forskolin and BPE. Two weeks later, the cells were characterized by immunohistochemical staining. Result:The shape of the cultured cells was mostly for 2~4 poles and there were few fibroblasts in them.The purity coefficient of OECs in these cultured cells was very high and the marker of OECs, p75 was positive in immunohistochemical staining. Conclusion:The condition of fractional cultivation of OECs was relatively harsh.The effect of purification the of cultured OECs with the method of immune adsorption by p75 was fine.

目的:探索分离培养人胚嗅鞘细胞以及纯化的方法。方法:分离人胚嗅球,采用胰酶消化获取单细胞悬液,应用含Forskolin、BPE的DMEM/F12培养基,接种于p75包被的培养皿,培养2周后细胞行免疫组化染色鉴定。结果:培养细胞形态多为2 ̄4极细胞,成纤维细胞少,嗅鞘细胞纯度非常高,嗅鞘细胞标志物p75阳性。结论:嗅鞘细胞体外分离培养条件要求极高,应用p75免疫吸附纯化培养的嗅鞘细胞效果好。

 
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