助手标题  
全文文献 工具书 数字 学术定义 翻译助手 学术趋势 更多
查询帮助
意见反馈
   cell checkpoint regulation 的翻译结果: 查询用时:0.009秒
图标索引 在分类学科中查询
所有学科
外科学
肿瘤学
更多类别查询

图标索引 历史查询
 

cell checkpoint regulation
相关语句
  “cell checkpoint regulation”译为未确定词的双语例句
     The study should shed light on eukaryotic cell checkpoint regulation and the origin of cancer.
     本研究有助于加深对真核细胞检查点调控的了解,为研究肿瘤的发生机制提供线索。
短句来源
     BRCA1 (breast cancer susceptibility gene 1) plays important roles in DNA damage repair,cell checkpoint regulation,gene transcription,chromosome stability,and apoptosis. At the C terminus of BRCA1 is the activation domain with a number of acidic amino acid residues that includes two tandem repeats of BRCT(BRCT1 and BRCT2).
     乳腺癌易感基因 (breastcancersusceptibilitygene 1,BRCA1)在DNA损伤修复、细胞周期调控、染色质的稳定、基因转录激活以及细胞凋亡等方面起着重要作用。
短句来源
  相似匹配句对
     The Checkpoint Regulation of Budding Yeast Cell Cycle
     芽殖酵母细胞周期的检查点调控
短句来源
     Micro Mechanisms of Cell Cycle Regulation and Checkpoint Control
     细胞周期调控的分子机制与关卡调控
短句来源
     The Role of Spindle Checkpoint Mechanism in the Regulation of Cell Cycle
     纺锤体检测点机制对细胞周期的调控
短句来源
     Replication and Regulation of Cell Centrosome
     细胞中心体的复制与调控
短句来源
     Cell Cycle and Regulation Factor.
     细胞周期和调控因子
短句来源
查询“cell checkpoint regulation”译词为用户自定义的双语例句

    我想查看译文中含有:的双语例句
例句
没有找到相关例句


WEB2 gene encodes a DNA helicase. A WEB2 mutant was treated with Hydroxyurea. Facs analysis, spindle microtubule staining by indirect immunofluorescence and % survial indicate that the mutant is defect in S phase checkpoint. We propose that WEB2 has not only helicase activity, but a role in S phase checkpoint regulation. And WEB2 gene locates in S phase checkpoint signal transduction pathway in S. cerevisiae. The study should shed light on eukaryotic cell checkpoint regulation and the origin of cancer.

WEB2基因编码产物为一种DNA解链酶。WEB2突变株经hydroxyurea阻断DNA合成后,经流式细胞仪检测DNA含量、纺锤体微管间接免疫 荧光染色及存活率测定,结果显示WEB2突变株表现S期检查点缺失。推测WEB2除了具有解链酶作用外,还参与酿酒酵母S期检查点调控机制,位于已建立的S期检查点信号传导通路模型上。本研究有助于加深对真核细胞检查点调控的了解,为研究肿瘤的发生机制提供线索。

BRCA1 (breast cancer susceptibility gene 1) plays important roles in DNA damage repair,cell checkpoint regulation,gene transcription,chromosome stability,and apoptosis.At the C terminus of BRCA1 is the activation domain with a number of acidic amino acid residues that includes two tandem repeats of BRCT(BRCT1 and BRCT2).In this study,to identify proteins that interact with the BRCT2 domain of BRCA1,the standard yeast two hybird screen was performed.FHL2 was isolated from a human ovary library,with...

BRCA1 (breast cancer susceptibility gene 1) plays important roles in DNA damage repair,cell checkpoint regulation,gene transcription,chromosome stability,and apoptosis.At the C terminus of BRCA1 is the activation domain with a number of acidic amino acid residues that includes two tandem repeats of BRCT(BRCT1 and BRCT2).In this study,to identify proteins that interact with the BRCT2 domain of BRCA1,the standard yeast two hybird screen was performed.FHL2 was isolated from a human ovary library,with the BRCT2 domain of BRCA1 as bait.Furthermore,the specific interaction of FHL2 with the BRCT2 domain of BRCA1,but not with the BRCT1 domain of BRCA1 and the BRCT domain of Rap1,was verified by yeast mating.To confirm the interaction between BRCA1 and FHL2 in vitro ,the GST pull down assay was performed,the coding sequences of BRCT1 and BRCT2 domains were fused in frame with the coding region of GST in the pGEX 2T vector,generating the pGST BRCT1 and pGST BRCT2 recombinant plasmids the fusion proteins GST BRCT1 and GST BRCT2 were expressed in E.coli DH5α.The purified fusion proteins were obtained by GST Sepharose 4B affinity chromatography.The purified fusion proteins were incubated with in vitro translated 35 S methinine labeled FHL2.Consistent with the two hybird results,FHL2 could specifically bind to the BRCT2 domain,but not BRCT1 in vitro. To further assess the binding specificity of FHL2 to the BRCT2 domain of BRCA1 in vivo ,pFLAG FHL2 and pHA BRCT1/ pHA BRCT2 recombinant plasmids were cotransfected into 293T cells.Then the coimmunoprecipitation assay were performed.The results also showed that FHL2 specifically interacted with the BRCT2 domain in vivo. Furthermore,the coimmunoprecipitation assay demonstrated that FHL2 could interact with endogenous BRCA1 in vivo. These findings lay solid foundations for study on the function of BRCA1 and FHL2 in cancer development and progression.

乳腺癌易感基因 (breastcancersusceptibilitygene 1,BRCA1)在DNA损伤修复、细胞周期调控、染色质的稳定、基因转录激活以及细胞凋亡等方面起着重要作用。BRCA1C 末端是富含酸性氨基酸的转录激活结构域 (AD) ,AD核心结构为两个串联的BRCT结构域 (BRCT1和BRCT2 )。应用酵母双杂交技术 ,以BRCT2为诱饵蛋白 ,从卵巢文库中筛选到了与BRCT2结构域相互作用蛋白FHL2 (fourandhalfLIMdomains)。利用酵母交配的方法证明FHL2与BRCA1BRCT2特异结合 ,而不与BRCA1BRCT1、Rap1BRCT结构域结合。GST沉淀实验表明 ,FHL2在体外特异地与BRCT2结构域相结合 ;免疫共沉淀实验表明 ,FHL2在体内特异地与BRCT2结构域结合 ;FHL2可与全长BRCA1结合。BRCA1与FHL2相互作用的发现为研究BRCA1以及FHL2在肿瘤发生、发展中的作用打下了坚实的基础。

 
图标索引 相关查询

 


 
CNKI小工具
在英文学术搜索中查有关cell checkpoint regulation的内容
在知识搜索中查有关cell checkpoint regulation的内容
在数字搜索中查有关cell checkpoint regulation的内容
在概念知识元中查有关cell checkpoint regulation的内容
在学术趋势中查有关cell checkpoint regulation的内容
 
 

CNKI主页设CNKI翻译助手为主页 | 收藏CNKI翻译助手 | 广告服务 | 英文学术搜索
版权图标  2008 CNKI-中国知网
京ICP证040431号 互联网出版许可证 新出网证(京)字008号
北京市公安局海淀分局 备案号:110 1081725
版权图标 2008中国知网(cnki) 中国学术期刊(光盘版)电子杂志社