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aer toxin
相关语句
  aer毒素
     Expression and Antigenicity Analysis of Aer Toxin of Aeromonas Hydrophila
     嗜水气单胞菌Aer毒素的表达与抗原性分析
短句来源
     Purification of Recombinant Aer Toxin and Antigenicity Analysis
     嗜水气单胞菌重组Aer毒素的纯化和抗原性分析
短句来源
     Cloning and sequence analysis of an aer toxin gene of Aeromonas hydrophila
     嗜水气单胞菌aer毒素基因的克隆及核苷酸序列分析
短句来源
     The nucleotide sequence showed 92% homology to the sequence of M16495.The nucleotide sequence was predicted to encode a 443-aa(amino acid) protein with the molecular weight of 53.9 ku and encode the mature aer toxin protein.
     分析推导其核苷酸编码的氨基酸序列显示:其编码443个氨基酸,为aer毒素成熟蛋白的基因,与M 16495相比,氨基酸差异主要集中在422氨基酸残基至436氨基酸残基的位置上,在这个位置上共有8个氨基酸残基发生变化,其中在435和437氨基酸残基之间有一个氨基酸密码子丢失,氨基酸的同源性达95.5%。 证明aer毒素基因的5′端保守,3′端变异较大。
短句来源
     First,the inclusion body of recombinant Aer toxin fusion protein by prokaryotic expression was purified by SDS-PAGE and electrophoresis elution. The natural Aer toxin of Aeromonas hydrophlia strain AHCS02 was purified by ammonium sulfate pricipatition.
     将原核表达的重组Aer毒素经SDS-PAGE后,切下目的蛋白,利用电洗脱法将蛋白洗脱出来,后经透析、PEG8000浓缩、定量,对獭兔进行免疫,采集血清,提纯IgG,在W estern b lotting试验中可与天然Aer毒素起反应,说明,体外表达的重组Aer毒素保留了天然Aer毒素所具有的抗原性。
短句来源
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  “aer toxin”译为未确定词的双语例句
     In this study, the Aer toxin gene was cloned and expressed in prokaryotic system and the antigenicity of its recombinant protein was analyzed.
     该领域国外研究热点在嗜水气单胞菌毒素的基因组结构、相关毒力因子基因克隆和序列比较以及毒素蛋白的表达和致病机理等方面。
短句来源
  相似匹配句对
     Purification of Recombinant Aer Toxin and Antigenicity Analysis
     嗜水气单胞菌重组Aer毒素的纯化和抗原性分析
短句来源
     Expression and Antigenicity Analysis of Aer Toxin of Aeromonas Hydrophila
     嗜水气单胞菌Aer毒素的表达与抗原性分析
短句来源
     Phytopathogenic Toxin
     植物病原毒素
短句来源
     2.toxin.
     2疫毒学说;
短句来源
     DR is more serious,AER is more serious.
     AER与DR的眼底改变呈显著的正相关性。
短句来源
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Antigencity of natural and recombinant Aer toxins of Aermonas hydrophila was analyzed by Western blotting.First,the inclusion body of recombinant Aer toxin fusion protein by prokaryotic expression was purified by SDS-PAGE and electrophoresis elution.The natural Aer toxin of Aeromonas hydrophlia strain AHCS02 was purified by ammonium sulfate pricipatition.Then both recombinant and natural Aer toxins were used to immunize rabbits to prepare anti-serum.Western blotting showed that the rabbit...

Antigencity of natural and recombinant Aer toxins of Aermonas hydrophila was analyzed by Western blotting.First,the inclusion body of recombinant Aer toxin fusion protein by prokaryotic expression was purified by SDS-PAGE and electrophoresis elution.The natural Aer toxin of Aeromonas hydrophlia strain AHCS02 was purified by ammonium sulfate pricipatition.Then both recombinant and natural Aer toxins were used to immunize rabbits to prepare anti-serum.Western blotting showed that the rabbit antibodies raised against the recombinant fusion protein could recognize the natural Aer toxin and the antibodies raised against natural Aer toxin could recognize the recombinant Aer toxin.The results indicated that natural and recombinant Aer toxins had similar antigenicity.

对原核表达的重组嗜水气单胞菌Aer毒素进行初步纯化,比较了它与天然Aer毒素的抗原性,为建立检测该毒素的免疫学方法奠定了基础。将原核表达的重组Aer毒素经SDS-PAGE后,切下目的蛋白,利用电洗脱法将蛋白洗脱出来,后经透析、PEG8000浓缩、定量,对獭兔进行免疫,采集血清,提纯IgG,在W estern b lotting试验中可与天然Aer毒素起反应,说明,体外表达的重组Aer毒素保留了天然Aer毒素所具有的抗原性。同时,将嗜水气单胞菌AHCS02菌接种于兔鲜血平板,30℃、培养15 h后,将具有β-溶血的单菌落于LB液体培养基中培养后,用饱和硫酸铵法提取上清液中的天然Aer毒素,用甲醛脱毒后,以同样免疫方法免疫獭兔,免疫结束后,采集血清提取IgG,在W estern b lotting试验中,可以检测到重组Aer毒素。试验说明:原核表达的重组Aer毒素和天然Aer毒素诱导獭兔产生的血清IgG抗体可以互相识别重组Aer毒素和天然Aer毒素,重组Aer毒素和天然Aer毒素有相似的抗原性。

A pair of primers was designed according the aer gene sequences in Genbank(accession No.M16495).With the specific primers,one target fragment about 1 332 bp was amplified from Aermonas hydrophila strain AHCS02 genomic DNA via PCR,which was isolated in Sijiazi reservoir,Jilin,China.The PCR products were cloned into pMD18-T vector,the amplified aer gene was sequenced and analyzed.The sequence was sent to the Genbank(accession No.AY136943) and compared with the corresponding regions of the M16495 by DNA tools software.The...

A pair of primers was designed according the aer gene sequences in Genbank(accession No.M16495).With the specific primers,one target fragment about 1 332 bp was amplified from Aermonas hydrophila strain AHCS02 genomic DNA via PCR,which was isolated in Sijiazi reservoir,Jilin,China.The PCR products were cloned into pMD18-T vector,the amplified aer gene was sequenced and analyzed.The sequence was sent to the Genbank(accession No.AY136943) and compared with the corresponding regions of the M16495 by DNA tools software.The nucleotide sequence showed 92% homology to the sequence of M16495.The nucleotide sequence was predicted to encode a 443-aa(amino acid) protein with the molecular weight of 53.9 ku and encode the mature aer toxin protein.

以自行分离的嗜水单胞菌分离株AHCS02为研究对象,分析其产生的aer毒素基因结构。根据G enB ank中aer毒素的基因序列(M 16495),设计扩增编码aer毒素成熟蛋白基因的引物,以AHCS02的基因组DNA为模板,PCR扩增出长度为1 332 bp的核苷酸片段,进行pM D 18-T载体克隆。酶切鉴定后进行序列测定和分析,结果表明扩增的片段与M 16495的同源性达92%,将测得的序列登录G enB ank数据库,所得的序列编号为AY 136943。分析推导其核苷酸编码的氨基酸序列显示:其编码443个氨基酸,为aer毒素成熟蛋白的基因,与M 16495相比,氨基酸差异主要集中在422氨基酸残基至436氨基酸残基的位置上,在这个位置上共有8个氨基酸残基发生变化,其中在435和437氨基酸残基之间有一个氨基酸密码子丢失,氨基酸的同源性达95.5%。证明aer毒素基因的5′端保守,3′端变异较大。

 
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