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dual target antisense rna
相关语句
  双靶区反义rna
     Objective: To study the effects of dual target antisense RNA against HBV infection.
     目的 :研究双靶区反义RNA的逆转录病毒载体的转染、表达及其抗乙肝病毒 (HBV)的作用。
短句来源
     Therefore the dual target antisense RNA may be potentially useful for anti HBV gene therapy than the single target antisense RNA.
     结论 :细胞内表达HBV反义RNA有明显抗乙肝病毒复制和表达的作用 ,而且双靶区反义RNA抗乙肝病毒的作用较单靶区反义RNA更明显。
短句来源
  “dual target antisense rna”译为未确定词的双语例句
     Construction of recombinant retrovirus vector carrying dual target antisense RNA of hepatitis B virus
     逆转录病毒载体介导双靶区乙型肝炎病毒反义RNA模型的构建
短句来源
     Methods:Recombinant retroviral vector expressing dual target antisense RNA complementary to HBV X(1400 1430), P(2375 2405) was used to infect the 2.2.15 cells. ELISA method was used to test the expression of HBV antigens.
     方法 :合成互补于HBVX区 (140 0 1430 )的X片段、互补于HBVP区 (2 375 2 40 5 )的P片段 ,分别构建表达单靶区反义X或P的重组逆转录病毒载体质粒和表达双靶区正义、反义X和P重组逆转录病毒载体质粒 ,转染PA317细胞 ,NIH3T3细胞扩增法测假病毒颗粒的滴度。
短句来源
     To probe the ways by which the antisense gene of HBV can be transcribed in eukaryotic cell to inhibit HBV replication and expression,the recombinant retrovirus vector carrying dual target antisense RNA of hepatitis B virus was constructed.
     构建在真核细胞内转录表达乙型肝炎病毒(HBV)双靶区反义核酸重组载体用以抗HBV基因治疗的研究。
短句来源
     The recombinant retrovirus vector carrying dual target antisense RNA of hepatitis B virus will be a good foundation for studying the inhibition of variable HBV in eukaryotic cell and for the antivirus treatment of polygenic disease.
     为探索在真核细胞内转录表达HBV反义RNA的方法及研究多基因区抗病毒治疗和抗变异病毒打下基础。
短句来源
  相似匹配句对
     N TARGET
     命中目标
短句来源
     The economic research target that ethics always is dual.
     经济伦理学的研究目标始终是双重的。
短句来源
     Location of acoustic target with dual right-triangles array
     用双直角三角形阵对声目标定位的研究
短句来源
     principle and target;
     原则和目标;
短句来源
     Dual Hypergroups
     对偶超群
短句来源
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Objective: To study the effects of dual target antisense RNA against HBV infection. Methods:Recombinant retroviral vector expressing dual target antisense RNA complementary to HBV X(1400 1430), P(2375 2405) was used to infect the 2.2.15 cells. ELISA method was used to test the expression of HBV antigens. Results: The results showed that the inhibitory rates of HBsAg in the 2.2.15 cells when incubated at 3, 5, 7, and 9 days after infected...

Objective: To study the effects of dual target antisense RNA against HBV infection. Methods:Recombinant retroviral vector expressing dual target antisense RNA complementary to HBV X(1400 1430), P(2375 2405) was used to infect the 2.2.15 cells. ELISA method was used to test the expression of HBV antigens. Results: The results showed that the inhibitory rates of HBsAg in the 2.2.15 cells when incubated at 3, 5, 7, and 9 days after infected by pLXSN+Xpos/Ppos group were 1%, 5%, 5%, and 11% respectively; by pLXSN+X group were 16%, 45%, 44%, and 38% respectively, pLXSN+P group were 34%, 59%, 55%, 51% respectively, pLXSN+X/P group were 73%, 83%, 81%, 79% respectively, and by the control group (pLXSN) were 5%, 4%, 6%, and 4% respectively .The inhibitory rates of HBeAg in the 2.2.15 cells when incubated 3, 5, 7, and 9 days after infected by pLXSN+Xpos/Ppos group were 6%,0,0,0 respectively, by pLXSN+X group were 13%, 53%, 52%, and 45% respectively, by pLXSN+P group were 21%, 57%, 56%, and 49% respectively; by pLXSN+X/P group were 62%, 87%, 84%, and 79% respectively. The inhibitory effects of all antisense RNA groups were higher than those of other groups and there was significant difference( P <0.01),especially in dual antisense RNA group (pLXSN+X/P) and no inhibitory effect between pLXSN and dual target sense RNA(pLXSN+Xpos/Ppos )groups. The viability of transduced 2.2.15 cells was not affected as indicated by MTT assays. Conclusion: The inhibitory effects of dual target antisense RNA group was higher than those of the single target antisense RNA group. Therefore the dual target antisense RNA may be potentially useful for anti HBV gene therapy than the single target antisense RNA.

目的 :研究双靶区反义RNA的逆转录病毒载体的转染、表达及其抗乙肝病毒 (HBV)的作用。方法 :合成互补于HBVX区 (140 0 1430 )的X片段、互补于HBVP区 (2 375 2 40 5 )的P片段 ,分别构建表达单靶区反义X或P的重组逆转录病毒载体质粒和表达双靶区正义、反义X和P重组逆转录病毒载体质粒 ,转染PA317细胞 ,NIH3T3细胞扩增法测假病毒颗粒的滴度。用假病毒颗粒感染 2 .2 .15细胞 ,用酶标法检测 2 .2 .15细胞上清HB sAg和HBeAg含量。 结果 :对HBsAg的抑制率在感染后第 3、5、7、9天时分别为 pLXSN :5 % ,4% ,6 % ,4% ;pLXSN +Xpos/Ppos:1% ,5 % ,5 % ,11% ;pLXSN +X :16 % ,45 % ,44 % ,38% ;pLXSN +P :34 % ,5 9% ,5 5 % ,5 1% ;pLXSN +X/P :73% ,83% ,81% ,79%。对HBeAg的抑制率在感染后第 3、5、7、9天时分别为pLXSN :2 % ,6 % ,4% ,3% ;pLXSN +Xpos/Ppo...

目的 :研究双靶区反义RNA的逆转录病毒载体的转染、表达及其抗乙肝病毒 (HBV)的作用。方法 :合成互补于HBVX区 (140 0 1430 )的X片段、互补于HBVP区 (2 375 2 40 5 )的P片段 ,分别构建表达单靶区反义X或P的重组逆转录病毒载体质粒和表达双靶区正义、反义X和P重组逆转录病毒载体质粒 ,转染PA317细胞 ,NIH3T3细胞扩增法测假病毒颗粒的滴度。用假病毒颗粒感染 2 .2 .15细胞 ,用酶标法检测 2 .2 .15细胞上清HB sAg和HBeAg含量。 结果 :对HBsAg的抑制率在感染后第 3、5、7、9天时分别为 pLXSN :5 % ,4% ,6 % ,4% ;pLXSN +Xpos/Ppos:1% ,5 % ,5 % ,11% ;pLXSN +X :16 % ,45 % ,44 % ,38% ;pLXSN +P :34 % ,5 9% ,5 5 % ,5 1% ;pLXSN +X/P :73% ,83% ,81% ,79%。对HBeAg的抑制率在感染后第 3、5、7、9天时分别为pLXSN :2 % ,6 % ,4% ,3% ;pLXSN +Xpos/Ppos :6 % ,0 ,0 ,0 ;pLXSN +X :13% ,5 3% ,5 2 % ,45 % ;pLXSN +P :2 1% ,5 7% ,5 6 % ,49% ;pLXSN +X/P :6 2 % ,87% ,84% ,79%。 结论 :细胞内表达HBV反义RNA有明显抗乙肝病毒复制和表达的作用 ,而且双靶区反义RNA抗乙肝病毒的作用较单靶区反义RNA更明显。

Objective To investigate the effect of ratroviral vector expressing the dual target antisense RNA of hepatitis B virus (HBV) on the replication and expression of HBV.Methods Recombinant retroviral vector plasmids expressing dual target antisense RNA complementary to HBV X (1400-1430) and P(2375-2405) were constructed and transduced into 2.2.15 cells. The experimental cells were divided into PLXSN+X, PLXSN+P, PLXSN+X/P, and PLXSN+Xpos/P pos groups. The HBV...

Objective To investigate the effect of ratroviral vector expressing the dual target antisense RNA of hepatitis B virus (HBV) on the replication and expression of HBV.Methods Recombinant retroviral vector plasmids expressing dual target antisense RNA complementary to HBV X (1400-1430) and P(2375-2405) were constructed and transduced into 2.2.15 cells. The experimental cells were divided into PLXSN+X, PLXSN+P, PLXSN+X/P, and PLXSN+Xpos/P pos groups. The HBV expression was tested by ELISA method. HBV DNA and RNA were tested by FQ PCR. Results The inhibition rates of 2.2.15 cells transduced with recombinant vector plasmids (PLXSN+X group, PLXSN+P group, especially the PLXSN+X/P group) on expression of HBV DNA and RNA were higher than those of blank control group, PLXSN group, and PLXSN+Xpos/Ppos group. The expression of the three ORFs, S, C, and P were significantly inhibited. Conclusion The expression of HBV antisense RNA in 2.2.15 cells can inhibit the replciation and expression of HBV. The inhibitory effect of dual antisense RNA group is higher than that of single antisense RNA group. Dual antisense RNA has the potentiality in anti HBV gene therapy.

目的 探讨表达乙型肝炎病毒 (HBV)双靶区反义RNA的逆转录病毒载体对HBV复制和表达的影响。方法 用分子克隆法构建表达HBVX、P双靶区正、反义RNA的重组载体质粒 ,转染PA317细胞 ,用NIH3T3细胞扩增法检测假病毒颗粒滴度 ,以假病毒颗粒感染 2 2 15细胞 ,用酶标法检测其上清HBsAg和HBeAg,并以荧光聚合酶链反应定量方法检测 2 2 15细胞内DNA和RNA含量。结果 HBVX、P双靶区反义RNA抗HBV的作用较单靶区反义RNA更明显 ,且对HBVS、C、P三个开放读码区的DNA和RNA抑制作用大 ,正义RNA无明显抑制作用。结论 细胞内表达HBV反义RNA具有明显抗HBV复制和表达的作用 ,双靶区反义RNA作用更明显。

Objective To investigate the curative effects of dual-target antisense RNA targeting the X and P regions in the genome of hepatitis B virus (HBV). Methods Retrovirus vector pLXSN was used to construct 4 kinds of recombinant vector plasmids expressing dual-target antisense RNA complementary to the X and P regions in the genome of HBV, namely, pLXSN-asX, pLXSN-asP, pLXSN-asXP, and pLXSN-seX. 48 HBV transgenic mice were randomly divided into 6 equal groups: pLXSN-asX...

Objective To investigate the curative effects of dual-target antisense RNA targeting the X and P regions in the genome of hepatitis B virus (HBV). Methods Retrovirus vector pLXSN was used to construct 4 kinds of recombinant vector plasmids expressing dual-target antisense RNA complementary to the X and P regions in the genome of HBV, namely, pLXSN-asX, pLXSN-asP, pLXSN-asXP, and pLXSN-seX. 48 HBV transgenic mice were randomly divided into 6 equal groups: pLXSN-asX group, pLXSN-asX group, pLXSN-asX group, pLXSN-asX group, and blank plasmid blank (pLXSN) group, to be injected into the caudal vein with corresponding plasmids trice for every other day, and blank control group. Venous blood samples were collected before, 1 day and 3 days, and 2, 4, and 8 weeks after the injection to undergo detection of serum HBV DNA and HBsAg. Eight weeks later the mice were killed and immunohistochemistry was used t examine the HBsAg and HBcAg in the tissues. Pathological examination of the tissues was performed. Results The serum HBsAg concentrations 4 and 8 weeks after injection were significantly lower than that before injection in the pLXSN-asX and pLXSN-asXP groups (all P<0.05) with an inhibition rate of 24% and 27% respectively. In comparison with that before injection, the HBV DNA expression level 2 weeks after injection was significantly lower in the pLXSN-asX group (P<0.05) with an inhibition rate of 58%. In comparison with that before injection, the HBV DNA expression level 8 weeks after injection was significantly lower in the pLXSN-asP group (P<0.05) with an inhibition rate of 58%. In comparison with that before injection, the HBV DNA expression level in the pLXSN-asXP group showed 2 times of significant decrease 1 week and 8 weeks after injection (both P<0.05) with the inhibition rates of 66% and 77% respectively. HBsAg and HBcAg were expressed in liver were significantly lower in the pLXSN-asX, pLXSN-asP, and pLXSN-asXP groups than in other groups (P<0.05). No significant abnormality was found in the tissues in all groups. Conclusion Dual-target antisense RNA targeting the X and P regions in the genome of HBV inhibits the replication and expression of HBV, significantly stronger than single-target antisense-RNA.

目的探讨针对乙型肝炎病毒(HBV)X、P双靶区反义RNA对乙型肝炎转基因小鼠HBV复制和表达的影响。方法构建表达HBV X、P双靶区正、反义RNA的重组载体质粒,与脂质体混合,经尾静脉注入小鼠体内,用酶联免疫法检测血清HBsAg;用荧光聚合酶链反应定量法检测血清HBV DNA含量;用免疫组化法检测肝细胞HBsAg、HBcAg的表达;小鼠心脏、肝脏、脾脏、肺脏、肾脏常规病理切片,观察反义RNA对小鼠脏器的影响。结果小鼠血清HBsAg表达,pLXSN-asX组和pLXSN-asXP组均于第四周明显低于给药前,最高抑制率分别达24%、27%;其余组未出现明显变化。与给药前相比,小鼠血清HBV DNA的复制,在pLXSN-asX组和pLXSN-asP组分别于第2周、第8周达到抑制高峰,抑制率均为58%;在pLXSN-asXP组于第1周、第8周出现两次抑制高峰,抑制率分别为66%、77%;其余组未出现明显变化。8周后,pLXSN-asX组、pLXSN-asP组和pLXSN-asXP组小鼠肝细胞HBsAg、HBcAg的表达显著低于其他组。小鼠脏器组织学观察未见异常。结论HBV X、P双靶区反义RNA对乙型肝炎病毒转基因鼠...

目的探讨针对乙型肝炎病毒(HBV)X、P双靶区反义RNA对乙型肝炎转基因小鼠HBV复制和表达的影响。方法构建表达HBV X、P双靶区正、反义RNA的重组载体质粒,与脂质体混合,经尾静脉注入小鼠体内,用酶联免疫法检测血清HBsAg;用荧光聚合酶链反应定量法检测血清HBV DNA含量;用免疫组化法检测肝细胞HBsAg、HBcAg的表达;小鼠心脏、肝脏、脾脏、肺脏、肾脏常规病理切片,观察反义RNA对小鼠脏器的影响。结果小鼠血清HBsAg表达,pLXSN-asX组和pLXSN-asXP组均于第四周明显低于给药前,最高抑制率分别达24%、27%;其余组未出现明显变化。与给药前相比,小鼠血清HBV DNA的复制,在pLXSN-asX组和pLXSN-asP组分别于第2周、第8周达到抑制高峰,抑制率均为58%;在pLXSN-asXP组于第1周、第8周出现两次抑制高峰,抑制率分别为66%、77%;其余组未出现明显变化。8周后,pLXSN-asX组、pLXSN-asP组和pLXSN-asXP组小鼠肝细胞HBsAg、HBcAg的表达显著低于其他组。小鼠脏器组织学观察未见异常。结论HBV X、P双靶区反义RNA对乙型肝炎病毒转基因鼠有显著抗HBV作用,且在作用效率和作用时间上优于单靶区反义RNA。

 
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