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culture and characterization
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  培养与鉴定
     Primary Culture and Characterization of Human Peritoneal Mesothelial Cells
     人腹膜间皮细胞的原代培养与鉴定卵巢癌细胞腹膜侵袭与转移机制的研究
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     Isolation, culture and characterization of porcine primordial germ cells
     猪原始生殖细胞的分离、培养与鉴定
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     Culture and characterization of human retinal capillary endothelial cell
     人类视网膜血管内皮细胞的培养与鉴定
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     In vitro culture and characterization of mouse spleen dendritic cells
     小鼠树突状细胞的体外培养与鉴定
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     Culture and characterization of endothelial progenitor cells from peripheral blood of rabbits
     兔外周血内皮祖细胞的培养与鉴定
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  “culture and characterization”译为未确定词的双语例句
     Method Neural stem cells were transfected by recombined rAAV2/eGFP and rAAV2/NGF virus after separa tion,culture and characterization,then we detected the expression of GFP and NGF by fluorescence microscopy and immunocytochemistry in vitro.
     方法 分离、培养和鉴定神经干细胞后,用重组rAAV2 /eGFP和rAAV2 /NGF病毒转染神经干细胞,用荧光显微镜和免疫细胞化学检测GFP和NGF在体外的表达。
     Method Neural stem cells were transfected by recombined rAAV2/eGFP and rAAV2/NGF virus after separa tion,culture and characterization,then we detected the expression of GFP and NGF by fluorescence microscopy and immunocytochemistry in vitro.
     方法 分离、培养和鉴定神经干细胞后 ,用重组rAAV2 /eGFP和rAAV2 /NGF病毒转染神经干细胞 ,用荧光显微镜和免疫细胞化学检测GFP和NGF在体外的表达。
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     Culture and Characterization of Infectious Bursal Disease Virus Variant BK912 Strain
     传染性囊病变异株病毒BK912的培育及鉴定
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     Culture and Characterization of Normal Rabbit Bladder Transitional Epithelial Cells in Vitro
     兔正常膀胱移行上皮细胞的体外培养和鉴定
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     Isolation,culture and characterization of Kuppffer cells from rat liver
     鼠肝枯否细胞的分离、培养和鉴定
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     Culture
     文化(英文)
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     Culture
     文化艺术
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     On Culture Process of Marxism Chinese-Characterization
     论马克思主义中国化的文化过程
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     Culture and characterization of human fetal hepatocytes
     人胚胎肝细胞的体外培养及表型观察
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     Culture and characterization of human peritoneal mesothelial cells
     人腹膜间皮细胞的培养及特征
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  culture and characterization
Isolation, culture and characterization of a primary fibroblast cell line from channel catfish
      
Culture and characterization of juvenile rabbit tenocytes
      
Culture and characterization of fetal human atrial and ventricular cardiac muscle cells
      
Culture and characterization of bovine mesenteric lymphatic endothelium
      
In this report, we present the isolation, culture and characterization of a new epithelial-like liver cell line (AKN-1) with a heterogeneous cell population and many characteristics of the biliary epithelium.
      
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This paper reports the in vitro culture and characterization of the morphology and growth of M. brassicae larval hemocytes. The in vitro culture was conducted with M-M media added with 0.02% TC-199, 0.06% tryptose and 20% FCS. The primary culture became confluent state in 35 days and 8 subcultures were performed. The cell colony was morphologically heterogenous. Most of the cells were spherical and spindle shaped. The spherical cells were 20-30μm in diameter, while the spindle cells were 18-20μm...

This paper reports the in vitro culture and characterization of the morphology and growth of M. brassicae larval hemocytes. The in vitro culture was conducted with M-M media added with 0.02% TC-199, 0.06% tryptose and 20% FCS. The primary culture became confluent state in 35 days and 8 subcultures were performed. The cell colony was morphologically heterogenous. Most of the cells were spherical and spindle shaped. The spherical cells were 20-30μm in diameter, while the spindle cells were 18-20μm wide by 35-50μm long. The multiplying time of a cell colony was about 60 hours at 27℃in the logarithmic period. The mode of chromosome was approximately spherical. The chromosome number varied considerably. The esterase isozyme exhibited 12 isozyme bands by PAGE with 6 major bands. The cultured cells could be directly freezed at 4℃and be stored for 70 days.

本文对甘蓝夜蛾血球细胞的离体培养,以及细胞系的形态特征和生长特性进行了初步研究。利用M—M培养液,辅之以0.02%TC-199、0.06%胰蛋白(月示)和20%胎牛血清,采用单层培养法培养甘蓝夜蛾老熟幼虫的血细胞。结果原代培养仅历时35天就开始了第一次传代,共继代培养8次。细胞形态以圆形和梭形为主,圆形细胞直径为20~31μm,梭形细胞大小为18~20μm×35~50μm。利用第三代培养的细胞悬液,以3.5×10~5cells/ml浓度培养,其群体倍增时间约60小时。核型分析表明,细胞的染色体约呈圆球形,数目变化较大。经聚丙烯酰胺凝胶电泳分析,细胞的酯酶同功酶带为12条,其中有6条主带。培养的细胞于4℃直接冷冻保存可长达70天。

The isolation,culture and characterization of embryonic stem(ES)cells in farm animals are very important for its research and usage.This article briefly reviewed the recent progress on various methods of isolation,culture and characterization of ES cells in farm animals.

家畜胚胎干细胞的分离培养和鉴定工作对于研究和利用家畜胚胎干细胞具有重大意义。本文对文献中报道的各种分离、培养和鉴定方法进行了综述 ,并介绍了几种主要家畜的胚胎干细胞在近十余年间的研究进展情况。

ObjectiveTo study the isolation, culture and characterization of neural stem cells from newborn rat spinal cords. Methods\ Cell culture technique and indirect immunoflurenscence cytochemistry were used. Results\ The cells were proliferated vitally. Neurospheres achieved by single cell cloning were positive with incoporation of BrdU and Nestin immunoflurescence staining. The cells had survived and grown for 8 months in vitro. The neurospheres could be induced to differentiate into neurons and astrocytes...

ObjectiveTo study the isolation, culture and characterization of neural stem cells from newborn rat spinal cords. Methods\ Cell culture technique and indirect immunoflurenscence cytochemistry were used. Results\ The cells were proliferated vitally. Neurospheres achieved by single cell cloning were positive with incoporation of BrdU and Nestin immunoflurescence staining. The cells had survived and grown for 8 months in vitro. The neurospheres could be induced to differentiate into neurons and astrocytes by 1% calf serum. Conclusions\ Neural stem cells from newborn rat spinal cords were cultured successfully.

目的 从新生大鼠的脊髓中分离培养神经干细胞并观察其增殖和分化能力。方法 采用细胞培养技术结合间接免疫荧光细胞化学法。结果 分离的细胞生长旺盛 ,单克隆化生成的细胞团 ,BrdU掺入呈强阳性。分离培养获得的细胞团呈Nestin强阳性 ,至今已在体外连续传代 8个月。培养的细胞团经 1%小牛血清诱导可分化为神经元和星形胶质细胞。结论 成功分离培养了新生大鼠脊髓神经干细胞

 
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